Updated on 2024/06/30

写真a

 
Miki Takeo
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Life and Environmental Sciences Environmental Science (Local Produce and Food Sciences) Associate Professor
Title
Associate Professor
Contact information
メールアドレス

Research History

  • 医学工学総合研究部 准教授

    2012.4

  • 医学工学総合研究部へ配置換

    2005.4

  • 山梨大学工学部附属ワイン科学研究センター転任

    2002.10

  • 山梨大学助手工学部

    1999.4

Education

  • Kyoto University

    - 1999.3

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    Country: Japan

  • Hokkaido University

    - 1993.3

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    Country: Japan

Degree

  • Doctor of Philosophy ( 2000.3   Kyoto University )

Research Areas

  • Life Science / Food sciences

  • Life Science / Applied microbiology  / Molecular Biology of the yeast

  • Life Science / Molecular biology

Research Interests

  • Genetics

  • Yeast

  • Cell biology

  • Environment

Subject of research

  • Molecular breeding of yeast

Research Projects

  • 高発酵性ワイン酵母を用いたバイオ発電に関する研究

    2005.4 - 2006.2

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    Authorship:Principal investigator  Type of fund::Donation

  • 産膜酵母の皮膜形成及び活性酸素消去機構制御に関する研究

    2002.9 - 2003.9

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    Authorship:Principal investigator  Type of fund::Others

  • キラー酵母の迅速な検出方法の構築

    2002.4 - 2003.2

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    Authorship:Principal investigator  Type of fund::Others

Papers

  • Wine Yeast Cells Acquire Resistance to Severe Ethanol Stress and Suppress Insoluble Protein Accumulation during Alcoholic Fermentation Reviewed

    Masashi Yoshida, Noboru Furutani, Futsuki Imai, Takeo Miki, Shingo Izawa

    Microbiology Spectrum   2022.8( ISSN:2165-0497 )

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    Language:English   Publishing type:(MISC) Research paper  

    DOI: https://doi.org/10.1128/spectrum.00901-22

  • Estimates in several industrial yeast strains ploidy by flow cytometer with rapamycin Reviewed Major achievement

    Journal of Brewing Society   110 ( 2 )   105 - 109   2015.2

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    Language:Japanese  

  • Construction of URA3 gene deletion mutant in Japanese wine yeast Saccharomyces cerevisiae OC-2 and investigation of its fermentation ability Reviewed Major achievement

    Journal of Brewing Society   108 ( 3 )   181 - 187   2013.3

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    Language:Japanese  

  • Vacuolar morphology of Saccharomyces cerevisiae during the process of wine making and Japanese sake brewing. Reviewed Major achievement

    Izawa S,Ikeda K,Miki T,Wakai Y,Inoue Y.

    Applied Microbiology and Biotechnology   88 ( 1 )   277 - 282   2010.9

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    Language:English  

  • PUT1遺伝子導入酵母株による甲州ブドウ果醪のプロリン資化 Reviewed Major achievement

    三木健夫,添川一寛,矢崎七瀬,石神裕康,猪狩信次,林幹雄

    日本醸造学会誌   105 ( 8 )   604 - 608   2010.5

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    Language:Japanese  

  • 日本産ブドウのアスコルビン酸含有量及びワイン発酵と貯蔵過程におけるアスコルビン酸濃度の変化 Reviewed Major achievement

    三木健夫・岩田祐輔・柳田藤寿・篠原 隆

    日本ブドウ・ワイン学会誌   19 ( 3 )   2008.12

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    Language:Japanese  

  • 甲州ワインおよびシャルドネワインに含まれるアミノ酸の分析と比較 Reviewed Major achievement

    三木健夫,篠原隆

    日本醸造協会誌   103 ( 12 )   954 - 958   2008.10

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    Language:Japanese  

  • Potentil of yeasts isolated in botrytized grape juice to be new wine yeasts Reviewed Major achievement

    Takeo Miki その他5名

    Food Science Technology Research   14 ( 4 )   345 - 350   2008.8

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    Language:English  

  • Formation of cytoplasmic P-bodies in Sake yeast during Japanese Sake brewing and wine making Reviewed Major achievement

    Shingo Izawa, Takeomi Kita, Kayo Ikeda, Takeo Miki, and Yoshiharu Inoue

    Bioscience Biotechnology Biochemistry   71 ( 11 )   2800 - 2807   2007.11

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    Language:English  

  • Study of L-proline consumption of PUT1 gene transformed yeast in Koshu grape juice Major achievement

    Takeo Miki, Nanase Yazaki, Kenjun Seki and Takashi Shinohara

    Journal of American Society Enology and Viticulture Japan   17 ( 3 )   184 - 185   2007.7

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    Language:Japanese  

  • 甲州ロゼワインの試験醸造について

    篠原 隆,時友裕紀子,三木健夫,柳田藤寿

    日本ワインブドウ学会誌   16 ( 3 )   116 - 117   2005.8

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    Language:Japanese  

  • Characterization of the export of bulk poly (A)+ mRNA in Saccharomyces cerevisiae during wine-making process Reviewed Major achievement

    Reiko Takemura,Takeo Miki,Yoshiharu Inoue,Shingo Izawa

    Applied and Environmental Microbiology   71 ( 4 )   2179 - 2182   2005.3

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    Language:English  

  • Ecological distribution and phenotypic diversity of Saccharomyces cerevisiae strains from wine-producing area in yamanashi, Japan Reviewed Major achievement

    Takashi Shinohara,HIrokazu Furuya,Fujitoshi Yanagida,Takeo Miki

    Microbiolgy Culture Collection   19   69 - 80   2003.12

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    Language:English  

  • Effect of tRCN1 gene on film formation of yeast Torulaspora delbrueckii Major achievement

    Takeo Miki, ,Kazuhiro Soekawa, ,Takashi Shinohara,Takafumi Naganuma

    Journal of ASEV JAPAN   15 ( 3 )   151 - 152   2003.10

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    Language:English  

  • Exploring film-forming genes of sherry yeast jerez-5. Major achievement

    K.Soekawa,T.Miki,T.Shinohara

    Journal of ASEV JAPAN   11 ( 3 )   165 - 166   2000.11

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    Language:English  

  • Effect of Pantothenic Acid Deficiency on Plasma Free Amino Acid Profile in Rainbow Trout Oncorhynchus mykiss. Reviewed Major achievement

    T. Masumoto, T. Miki, H. Hosokawa and S. Shimeno

    Fisheries Science   65   749 - 795   1999.6

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    Language:English  

  • Importance of glucose-6-phosphate dehydrogenase in the adaptive response to hydrogen peroxide in Saccharomyces cerevisiae Reviewed Major achievement

    Shingo Izawa,Keiko Maeda,Takeo Miki,Junichi Mano,Yoshiharu Inoue,Akira Kimura

    Biochem. J.   330   811 - 817   1998.6

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    Language:English  

  • Oxidative stress response in yeast: Purification and some properties of oxidative stress-inducible glucose-6-phosphate dehydrogenase from Hansenula mrakii Reviewed Major achievement

    Takeo Miki,Yoshiyuki Tsujimoto,Shinji Miyabe,Kei-ichi Sugiyama,Shingo Izawa,Yoshiharu Inoue,Akira Kimura

    Biosci.Biotech.Biochem.   60 ( 6 )   966 - 970   1996.8

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    Language:English  

  • Oxidative Stress Response in Yeast: Glutathione peroxidase of Hansenula mrakii is bound to the membrane of both mitochondria and cytoplasm Reviewed Major achievement

    Yoshiharu Inoue, Linh.-Thuoc Tran, ,Masaki Kamakura, ,Shingo Izawa, ,Takeo Miki, ,Yoshiyuki Tsujimoto and Akira Kimura

    Biosci.Biotech.Biochem.   60 ( 6 )   966 - 970   1996.6

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    Language:English  

  • Oxidative stress response in Yeast: Purification and characterization of glutathione reductase from Hansenula mrakii Reviewed Major achievement

    Takeo Miki,Yoshiyuki Tsujimoto,Shinji Miyabe,Kei-ichi Sugiyama,Shingo Izawa,Yoshiharu Inoue,Akira Kimura

    Biosci.Biotech.Biochem.   60 ( 7 )   1207 - 1209   1996

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    Language:English  

  • Oxidative stress response in yeast: Induction of glucose-6-phosphate dehydrogenase by lipidhydroperoxide in Hansenula mrakii Reviewed Major achievement

    Linh.-Thuoc Tran, ,Takeo Miki, ,Masaki Kamakura, Shingo Izawa, ,Yoshiyuki Tsujimoto, ,Shinji Miyabe, ,Yoshiharu Inoue and Akira Kimura

    J. Ferment. Bioeng.   80   606 - 609   1995.10

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    Language:English  

  • Oxidative Stress Response in Yeast: Glutathione peroxidase of Hansenula mrakii is bound to the membrane of both mitochondria and cytoplasm Reviewed Major achievement

    Yoshiharu Inoue,Linh.-Thuoc Tran,Masaki Kamakura,Shingo Izawa,Takeo Miki,Yoshiyuki Tsujimoto,Akira Kimura

    Biochem. Biophys. Acta.   1245   325 - 330   1995.6

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    Language:English  

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Books and Other Publications

  • ワイン酵母と遺伝子 Major achievement

    三木健夫(-)

    山梨日日新聞  2008.8 

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    Responsible for pages:-   Language:Japanese  

  • 海外渡航報告書(第八回国際ワイン醸造学会への参加とポスター発表) Major achievement

    三木健夫(-)

    山梨工業会  2008.3 

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    Total pages:1   Responsible for pages:17-17   Language:Japanese  

Presentations

  • 酵母細胞の高温ストレスによる活性酸素発生量に関する研究

    佐野絢子、三木健夫

    日本生物工学会年次大会(オンライン)  2022.10 

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 高浸透圧条件における酵母OCM-2株の脂肪酸組成の変化

    三木健夫、佐野絢子

    日本生物工学会年次大会(オンライン)  2022.10  日本生物工学会

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ソルビトールストレスによる GPD遺伝子破壊株の脂肪酸組成変化 Major achievement

    三木健夫、佐野絢子、井沢真吾

    日本生物工学会  2021.10 

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ソルビトールストレスが酵母OC-2株の脂肪酸構成に与える影響

    三木健夫

    2019.9  日本生物工学会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:岡山  

  • 酵母Saccharomyces cerevisiae YM41株の保持するキラー遺伝子の取得 ~第二報~

    三木 健夫、篠笥 光慶

    日本生物工学会  2018.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:大阪  

  • Effect of osmotic stress on fatty acid component in wine yeast S. cerevisiae OC-2 Major achievement

    2015.10 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Estimation in several industrial yeast strains ploidy by flow cytometer with rapamycin Major achievement

    2014.9 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Making a low proline level Koshu grape wine by PUT genes transformed wine yeast. Major achievement

    2012.3 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ワイン酵母Saccharomyces cerevisiae OC-2株におけるPYC2遺伝子産物の細胞内局在性について Major achievement

    三木健夫,大家尋俊

    日本農芸化学会誌  2010.3  日本農芸化学会

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:東京大学  

  • ワイン酵母S. cerevisiae OC-2株におけるPYC2遺伝子産物の細胞内局在性について Major achievement

    三木健夫,大矢尋俊

    日本ワイン・ブドウ学会誌  2009.11  日本ワイン・ブドウ学会

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    Event date: 2009.11

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:広島大学  

  • ワイン酵母のPYC2遺伝子産物の細胞内局在性の変化 Major achievement

    三木健夫,黒羽一誠,篠原隆

    2009.3  日本農芸化学会

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:福岡  

  • ワイン酵母株間でのプロリン消費の差異について Major achievement

    三木 健夫,篠原 隆

    日本ワイン・ブドウ学会  2008.7 

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    Event date: 2008.7

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:山形県 山形テルサ  

  • PUT1遺伝子導入株による甲州ワインのプロリン濃度の制御 Major achievement

    三木 健夫,矢崎 七瀬,石 研順,篠原 隆

    日本ブドウ・ワイン学会  2007.11 

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    Event date: 2007.11

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:山梨県甲府市  

  • The effect of ammonium addition on amino acid, ethanol and aromatic compound production by wine yeast strains (Saccharomyces cerevisiae) in fermentation of Koshu grape Major achievement

    Shinohara T., Nakane M. and Miki T.

    OENO2007  2007.6  ボルドー大学

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    Event date: 2007.6

    Language:English   Presentation type:Oral presentation(general)  

    Venue:フランス ボルドー大学  

  • PUT1遺伝子導入酵母株のプロリン消費に関する研究 Major achievement

    三木健夫,矢崎七瀬,篠原隆

    大会講演要旨集  2007.3  日本農芸化学会

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:東京  

  • 高浸透圧条件下でのワイン酵母PYC2遺伝子発現の変化 Major achievement

    黒羽一誠,三木健夫,篠原 隆

    日本農芸化学会誌  2006.3  日本農芸化学会

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:京都女子大学  

  • ワイン酵母のプロリン消費に関する研究 Major achievement

    三木健夫,黒羽一誠,篠原 隆

    日本農芸化学会  2006.3  日本農芸化学会

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    Event date: 2006.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:京都女子大学  

  • ワイン酵母KN-35,KN-94株のストレス耐性に関する研究 Major achievement

    三木健夫,伊藤義晃,黒羽一誠,望月舞雪,篠原隆

    日本農芸化学会大会(札幌)  2005.3 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ワイン酵母の高浸透圧条件下における糖代謝の変化 Major achievement

    黒羽一誠,望月舞雪,小田切正典,三木健夫,篠原隆,下飯仁

    日本農芸化学会大会(札幌)  2005.3 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ワイン酵母YM41株のキラータンパク質の作用機序に関する研究 Major achievement

    小田切正典,竹内美貴,黒羽一誠,望月舞雪,三木健夫,篠原隆

    日本農芸化学会大会(札幌)  2005.3 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 高発酵性ワイン酵母の選択および試験醸造 Major achievement

    篠原 隆,伊藤 義晃,三木 健夫,添川 一寛,猪狩 信次,林 幹雄,柳田 藤寿

    日本ワイン・ブドウ学会(東京)  2003.11 

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    Event date: 2003.11

    Language:English   Presentation type:Oral presentation(general)  

  • 産膜酵母のtRCN1遺伝子が皮膜形成に及ぼす影響 Major achievement

    三木 健夫,添川 一寛,篠原 隆,長沼 孝文

    日本ワイン・ブドウ学会(東京)  2003.11 

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    Event date: 2003.11

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 産膜酵母Torulaspora delbrueckiiのtRCN1遺伝子の機能解析 Major achievement

    三木 健夫,篠原 隆,長沼 孝文

    日本生物工学会(熊本)  2003.9 

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    Event date: 2003.9

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Relationship between high fermentation ability and environmental stress tolerance of wine yeast. Major achievement

    Shinohara T., Miki T. and Ito Y.

    VII th International Symposium of Oenology of Bordeaux 2003  2003.6 

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    Event date: 2003.6

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:フランス  

  • ワイン酵母S. cerevisiae YM41株のキラータンパク質に関する研究 Major achievement

    三木健夫,篠笥光慶,篠原 隆

    日本農芸化学会大会(東京)  2003.3 

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    Event date: 2003.3

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 産膜酵母Torulaspora delbrueckiiの皮膜形成に関する研究 Major achievement

    三木健夫,小森英美,添川一寛,篠原 隆

    日本農芸化学会大会(仙台)  2002 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ワイン酵母の生産するキラータンパク質の精製  Major achievement

    篠笥光慶,竹内美貴,三木健夫,篠原 隆

    日本農芸化学会大会(仙台)  2002 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 高発酵性ワイン酵母の評価とストレス耐性との関連性 Major achievement

    伊藤義晃,三木健夫,篠原 隆

    日本ワイン・ブドウ学会大会(甲府)  2001 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 産膜酵母Torulaspora delbrueckiiの皮膜形成に関わる遺伝子の探索 Major achievement

    三木健夫,添川一寛,篠原 隆

    日本農芸化学会大会(京都)  2001 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Green fluorescent proteinを用いたキラー酵母検出の試み  Major achievement

    三木健夫,青木康宏,篠原 隆

    日本生物工学会大会(札幌)  2000.8 

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    Event date: 2000.8

    Language:Japanese   Presentation type:Oral presentation(general)  

  • シェリー酵母Jerez-5の皮膜形成に関わる遺伝子の探索 Major achievement

    添川一寛,三木健夫,篠原 隆

    日本ワイン・ブドウ学会大会(千葉)  2000 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation(general)  

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Awards

  • 日本農芸化学会関東支部会若手研究奨励賞

    2008.10   日本農芸化学会  

    三木健夫,篠原隆

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    甲州ワインに含まれるプロリンの低減化に関する研究

Teaching Experience (On-campus)

  • Topics of Food Science

    2023Year

  • Environmental and Symbiotic Biology

    2023Year

  • Introduction to Symbiotic Science

    2023Year

  • Laboratory Training of Local Produce and Food Science II

    2023Year

  • Laboratory Training of Local Produce and Food Science I

    2023Year

  • Food Preservation Major achievement

    2023Year

  • Research on Regional Environment Management A

    2023Year

  • Seminar on Regional Environment Management A

    2023Year

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Guidance results

  • 2023

    Type:Undergraduate (Major A course)graduation thesis guidance  Period:1months  Year guidance time:360hours

    Number of people receiving guidance :2people  (Overseas students):0people

    Graduation / pass / number of people awarded degrees :2people 

    Number of teachers:1people

  • 2022

    Type:Undergraduate (Major A course)graduation thesis guidance  Period:11months  Year guidance time:360hours

    Number of people receiving guidance :2people 

    Graduation / pass / number of people awarded degrees :2people 

    Number of teachers:1people

  • 2022

    Type:Master's (Major B course)dissertations guidance  Period:11months  Year guidance time:360hours

    Number of people receiving guidance :1people 

    Graduation / pass / number of people awarded degrees :1people 

    Number of teachers:1people

  • 2021

    Type:Undergraduate (Major A course)graduation thesis guidance  Period:11months  Year guidance time:360hours

    Number of people receiving guidance :3people 

    Graduation / pass / number of people awarded degrees :3people 

    Number of teachers:1people

  • 2021

    Type:Master's (Major B course)dissertations guidance  Period:24months  Year guidance time:720hours

    Number of people receiving guidance :1people 

    Number of teachers:1people

  • 2020

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :3people 

    Graduation / pass / number of people awarded degrees :3people 

    Number of teachers:1people

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Review of master's and doctoral thesis

  • 2022

    Examiner classification:Chief examiner

    Master :1people 

Performance of external announcement guidance

  • 2022

    Japanese papers guidance - Oral presentation - Number of students:1people  (Guidance time):30hours

Social Activities

  • 令和3年度山梨県農業クラブ連盟プロジェクト発表大会 審査員

    Role(s): Advisor

    2022.3

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    Audience: High school students

    Type:Other

Professional Memberships

  • 日本食品科学工学会

    2010.5

  • 日本生物工学会

    1996.5

  • 日本農芸化学会

    1996.4