Updated on 2024/08/13

写真a

 
Aoki Yoshinao
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Life and Environmental Sciences Bioagronomy (Local Produce and Food Sciences, The Institute of Enology and Viticulture) Assistant Professor
Title
Assistant Professor
Profile
■植物分子・植物病理分野の基礎研究  「植物と病害菌との相互関係の解明」 ■園芸学分野の応用研究  「病害菌の防除など農業現場へ還元できる研究」 <主な研究テーマ> 【1】ブドウ病害菌を防除する有効物質の探索 【2】ブドウ樹の微生物叢の調査 【3】日本独自のブドウ用台木の作成 醸造用ブドウを対象に、上記の研究を行っています

Research History

  • University of Yamanashi   Assistant Professor

    2022.4

  • University of Yamanashi   Department of Research Interdisciplinary Graduate School of Medicine and Engineering Institute of Enology and Viticulture   Assistant Professor

    2021.4

      More details

    Country:Japan

  • University of Yamanashi

    2016.4 - 2021.3

  • University of Yamanashi   Department of Research Interdisciplinary Graduate School of Medicine and Engineering Institute of Enology and Viticulture

    2016.4 - 2021.3

      More details

    Country:Japan

  • University of Yamanashi

    2015.4 - 2016.3

▼display all

Education

  • University of Yamanashi

    2012.4 - 2015.3

      More details

    Country: Japan

    Course: Doctor course

  • University of Yamanashi   Department of Education Interdisciplinary Graduate School of Medicine and Engineering

    2012.4 - 2015.3

      More details

    Country: Japan

  • University of Yamanashi

    2010.4 - 2012.3

      More details

    Country: Japan

    Course: Master course

  • University of Yamanashi   Department of Education Interdisciplinary Graduate School of Medicine and Engineering

    2010.4 - 2012.3

      More details

    Country: Japan

  • Teikyo University

    2006.4 - 2010.3

      More details

    Country: Japan

    Course: Others

▼display all

Degree

  • 博士(工学) ( 2015.3   山梨大学 )

Research Areas

  • Environmental Science/Agriculture Science / Plant protection science

  • Environmental Science/Agriculture Science / Horticultural science

  • Life Science / Molecular biology

  • Environmental Science/Agriculture Science / Plant protection science  / 遺伝子診断

  • Environmental Science/Agriculture Science / Horticultural science  / microbial pesticides

Research Interests

  • 微生物農薬

  • 植物ウイルス

  • 薬剤耐性

  • 植物病原菌

  • 醸造用ブドウ

  • gene diagnosis

  • Plant pathology

  • Plant protection

  • Microbial pesticides

Subject of research

  • 日本独自のブドウ用台木の作成

  • ブドウ樹の微生物叢の調査

  • ブドウ病害菌を防除する有効物質の探索

Research Projects

  • 日本独自のブドウ根頭がんしゅ病耐性台木の育種

    2023.4 - 2024.3

    公益財団法人東洋食品研究所  2022年度 一般研究助成  育種

    青木是直

      More details

    Authorship:Principal investigator  Type of fund::Science research expense

  • 山梨オーガニックワイン推進コンソーシアムが目指す減農薬ブドウ栽培支援 システム -’超’早期病原菌検出の試み -

    2022.7 - 2023.3

    山梨大学  令和4年度 地域貢献促進プロジェクト  植物保護

    青木是直、鈴木俊二、金成 葉子、伊東 和彦、井戸 隆一

      More details

    Authorship:Principal investigator  Type of fund::Science research expense

  • テロワールを構成する内的要因:ブドウ樹内マイクロバイオームの品種および地域間解析

    Grant number:22K14977  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業  若手研究

    青木 是直

  • テロワールを構成する内的要因:ブドウ樹内マイクロバイオームの品種および地域間解析

    2022.4 - 2024.3

    日本学術振興会  科学研究費助成事業 若手研究  2022年度 若手研究

    青木是直

      More details

    Authorship:Principal investigator  Type of fund::Science research expense

  • Microflora in grape

    2020.8 - 2021.3

    The Institute of Enology and Viticulture, University of Yamanashi 

▼display all

Papers

  • Identification of a new gibberellin receptor agonist, diphegaractin, by a cell-free chemical screening system. Reviewed

    Akira Nozawa, Ryoko Miyazaki, Yoshinao Aoki, Reina Hirose, Ryosuke Hori, Chihiro Muramatsu, Yukinori Shigematsu, Keiichirou Nemoto, Yoshinori Hasegawa, Keiko Fujita, Takuya Miyakawa, Masaru Tanokura, Shunji Suzuki, Tatsuya Sawasaki

    Communications biology   6 ( 1 )   448 - 448   2023.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Gibberellin (GA) is a phytohormone that regulates various developmental processes during the plant life cycle. In this study, we identify a new GA agonist, diphegaractin, using a wheat cell-free based drug screening system with grape GA receptor. A GA-dependent interaction assay system using GA receptors and DELLA proteins from Vitis vinifera was constructed using AlphaScreen technology and cell-free produced proteins. From the chemical compound library, diphegaractin was found to enhance the interactions between GA receptors and DELLA proteins from grape in vitro. In grapes, we found that diphegaractin induces elongation of the bunch and increases the sugar concentration of grape berries. Furthermore, diphegaractin shows GA-like activity, including promotion of root elongation in lettuce and Arabidopsis, as well as reducing peel pigmentation and suppressing peel puffing in citrus fruit. To the best of our knowledge, this study is the first to successfully identify a GA receptor agonist showing GA-like activity in agricultural plants using an in vitro molecular-targeted drug screening system.

    DOI: 10.1038/s42003-023-04760-y

    PubMed

  • Diketopiperazine cyclo (-l-Leu-l-Phe) with plant elicitation activity and anti-oomycete activity against Plasmopara viticola. Reviewed

    Aoki, Y., Kunitomi, M., Mori, A., Watanabe, G., Nojiri, M., Suzuki, S.

    LETTERS IN APPLIED MICROBIOLOGY   2022.12( ISSN:0266-8254 )

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    The aim of this study was to contribute to the reduction of synthetic chemical fungicide application in viticulture by using cyclo(-l-Leu-l-Phe) (cLF) produced by Bacillus subtilis KS1, a candidate for biological control agent. cLF is a diketopiperazine and belongs to the family of 2,5-diketopiperazines. KS1 secreted micromolar levels of cLF into culture medium. Micromolar concentrations of cLF structure-dependently decreased by ∼90% both disease severity and lesion density of downy mildew in grapevine cultivated in a growth chamber. Microscopic observation demonstrated that cLF inhibited Plasmopara viticola haustorium formation by 80% but not zoospore germination on leas disks. Interestingly, millimolar concentrations of cLF induced plant defense response, such as expression of genes encoding chitinase and β-1,3-glucanase, in grapevine leaves through the salicylic acid and jasmonate signaling pathways. We demonstrated that cLF was a weapon against P. viticola infection. Further evaluation of cLF in field trials is required to uncover its inherent characteristics.

  • Diketopiperazine cyclo(-l-Leu-l-Phe) with plant elicitation activity and anti-oomycete activity against Plasmopara viticola Reviewed

    Aoki, Y, Kunitomi, M, Mori, A, Watanabe, G, Nojiri, M, Suzuki, S

    2022.12

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/lambio/ovac039

  • Diversity of endophytic bacterial microbiota in grapevine shoot xylems varies depending on wine grape-growing region, cultivar, and shoot growth stage. Reviewed

    Hamaoka, K., Aoki, Y., Takahashi, S., Enoki, S., Yamamoto, K., Tanaka, K., Suzuki, S.

    Scientific Reports   2022.9( ISSN:2045-2322 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

  • Diversity of endophytic bacterial microbiota in grapevine shoot xylems varies depending on wine grape-growing region, cultivar, and shoot growth stage.

    Kazuhiro Hamaoka, Yoshinao Aoki, Sayuri Takahashi, Shinichi Enoki, Kosuke Yamamoto, Keisuke Tanaka, Shunji Suzuki

    Scientific reports   12 ( 1 )   15772 - 15772   2022.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Next-generation sequencing technology may clarify microbiota that are as yet poorly understood in the soil, the rhizosphere, and the phyllosphere of vineyards. To provide new information on the interaction between grapevine and microorganisms, we focused on the endophytic microbiota in grapevine. We performed endophytic microbiome analysis of the shoot xylems of four cultivars, Vitis vinifera cvs. Chardonnay, Pinot Noir, Cabernet Sauvignon, and Vitis sp. cv. Koshu, grown in eleven vineyards in Japan. The number of endophytic fungal species was small in the grapevine shoot xylems and could not be analyzed further, whereas a total of 7,019,600 amplicon sequences (46,642-285,003 per shoot xylem) and 1305 bacterial operational taxonomic units were obtained by analysis of the V3-V4 region of the bacterial 16S rRNA gene. Gammaproteobacteria was predominant in the shoot xylems at the shoot elongation stage irrespective of the cultivar, whereas Alphaproteobacteria and Oxyphotobacteria were predominant at véraison. Actinobacteria, Bacteroidia, Bacilli, and Clostridia were also detected in the shoot xylems. The endophytic bacterial microbiota in Koshu and Pinot Noir shoot xylems were similar irrespective of the grapevine-growing region. In contrast, the endophytic bacterial microbiota in Chardonnay and Cabernet Sauvignon showed diversity and complexity among grapevine-growing regions. Alpha diversity analysis revealed that Koshu shoot xylems had a higher diversity of endophytic bacterial microbiota than Pinot Noir, Chardonnay, and Cabernet Sauvignon shoot xylems, and that grapevine shoot xylems at the shoot elongation stage had a higher diversity of endophytic bacterial microbiota than those at véraison. Principal coordinate analysis (PCoA) demonstrated that the profiles of the endophytic bacterial microbiota in grapevine shoot xylems at véraison were relatively uniform compared with those at the shoot elongation stage. Multidimensional scaling analysis showed that the plots of all cultivars were generally apart from each other at the shoot elongation stage and then became close to each other at véraison. The plots of all grapevine-growing regions cultivating Koshu were close to each other, whereas those of grapevine-growing regions cultivating Chardonnay and Cabernet Sauvignon were apart from each other. The findings of this study suggest that the endophytic bacterial microbiota in grapevine shoot xylems varied depending on the cultivar and the grapevine-growing region even for the same cultivars, and that the microbiota fluctuated depending on the shoot growth stage.

    DOI: 10.1038/s41598-022-20221-8

    PubMed

▼display all

Presentations

  • 遺伝子診断法を用いた薬剤耐性ブドウべと病菌のモニタリング

    青木是直, 鈴木俊二

    平成29年度植物病理学会関東部会  2017.9 

     More details

    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 減農薬栽培に向けたカルボン酸アミド系薬剤耐性ブドウべと病菌のモニタリング

    青木是直, 河越由美, 田中紗弓, 鈴木俊二

    平成26年度園芸学会秋季大会  2014.9 

     More details

    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Multiplex ASP-PCR法によるQoIおよびCAA殺菌剤耐性ブドウべと病菌簡易判定法の確立

    青木是直, 羽田洋祐, 望月真衣, 鈴木俊二

    平成24年度日本植物病理学会大会  2012.3 

     More details

    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation(general)  

Teaching Experience (On-campus)

  • Vineyard Practice

    2023Year

  • Viticultural Science Major achievement

    2023Year

  • Research on Food and Wine Science A

    2023Year

  • Seminar on Food and Wine Science A

    2023Year

  • Topics of Food Science

    2023Year

▼display all

Guidance results

  • 2023

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :4people 

  • 2023

    Type:Achievement of student guidance (Undergraduate)

    Number of people receiving guidance :4people 

  • 2022

    Type:Achievement of student guidance (Undergraduate)

    Number of people receiving guidance :3people 

  • 2022

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :3people 

Social Activities

  • 醸造用ブドウの栽培方法と圃場での実例

    Role(s): Lecturer

    2024.8

  • 農作物の病気について

    Role(s): Lecturer

    2024.8

  • 山梨オーガニックワイン推進コンソーシアムが目指す減農薬ブドウ栽培支援システム -’超’早期病原菌検出の試み -

    Role(s): Investigater

    2022.7 - 2023.3

Professional Memberships

  • American society for enology & viticulture Japan chapter

    2014.4

  • American society for enology and viticulture (ASEV)

    2014.4

  • The Phytopathological Society of Japan

    2010.8

  • 日本植物病理学会

  • 日本ブドウ・ワイン学会(ASEV Japan)