Faculty Profiles - Otsuki Takashi

写真a

Otsuki Takashi

Organization

Graduate Faculty of Interdisciplinary Research Faculty of Life and Environmental Sciences Bioagronomy (Biotechnology) Associate Professor

Contact information

Homepage

http://www.ccn.yamanashi.ac.jp/~tohtsuki/bt4/index.html

Research History

University of Yamanashi Graduate Faculty of Interdisciplinary Research Faculty of Education, Education (Childhood developmental education) Associate Professor

2014.11
University of Yamanashi Department of Research Interdisciplinary Graduate School of Medicine and Engineering Associate Professor

2011.4 - 2014.10
University of Yamanashi Department of Research Interdisciplinary Graduate School of Medicine and Engineering Assistant Professor

2007.4 - 2011.9
University of Yamanashi Department of Research Interdisciplinary Graduate School of Medicine and Engineering Research Associate

2003.4 - 2007.3
University of Yamanashi Faculty of Engineering Research Associate

1999.4 - 2003.3
The University of Tokyo Visiting Researcher

1997.10 - 1999.3
The University of Tokyo The Institute of Medical Science

1997.10
The University of Tokyo

1997 - 1999

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Education

Shinshu University

1994.4 - 1997.9

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Country： Japan

Course： Doctor later
Shinshu University

1992.4 - 1994.3

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Country： Japan
Shinshu University

1988.4 - 1992.3

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Country： Japan

Degree

Ph.D. ( 1997.9 Shinshu University )
M.Agr. ( 1994.3 Shinshu University )
B.Agr. ( 1992.3 Shinshu University )

Research Areas

Life Science / Molecular biology
Environmental Science/Agriculture Science / Environmental agriculture
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
Life Science / Applied microbiology

Research Interests

Environmental-friendry supply of biofuel
Biotechnology responsible to zero-emmission society
Production of valuable compounds
Effective utilization of microbial community

Subject of research

Production of useful compounds including chemicals, bioactive compounds and biofuels
Utilization of various functions of microorganisms
Utilization of network in microbial community
Utilization of biomass resources

Research Projects

「富⼠の国やまなし微⽣物ライブラリー」の構築と「微⽣物ジェネラリスト」の育成・教育

2023.4 - 2026.3

発酵研究所発酵研究所研究室助成

田中靖浩、山村英樹、大槻隆司、乙黒美彩、片岡良太

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Authorship：Coinvestigator(s) Grant type：Competitive Type of fund：：Donation
刈草特化型メタン発酵プロセス開発のための基礎検討

Grant number：23K05489 2023.4 - 2026.3

日本学術振興会科学研究費助成事業基盤研究(C)（一般）

大槻隆司

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Authorship：Principal investigator Grant type：Competitive Type of fund：：Science research expense
Biohydrogen production from wheat straw enhanced by starch addition

Grant number：25450370 2013.4 - 2016.3

Japan Society for the Promotion of Science University of Yamanashi Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)

OHTSUKI Takashi

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Cells of Clostridium butyricum, C. cellulovorans, C. perfringens were grown on GS medium containing 1% wheat straw powder, and the enhancing effect of starch addition on hydrogen production was investigated. Hydrogen production by all Closrtidia tested, derived from wheat straw, were reached 4-fold volume compared to the culture without starch addition.
Activities of amylases and cellulases from C. cellulovorans culture with starch addition were increased up to 19.2-fold than the culture without starch addition, however, degradation of holocellulose in wheat straw was not enhanced. These results strongly suggested that sugar metabolism of C. cellulovorans would be altered to high hydrogen-yielding state.
Direct conversion of plant biomass to isoprene by microorganisms expressing cell-wall degrading enzymes from parasitic plants

Grant number：23656527 2011 - 2012

Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research

OKAZAWA Atsushi, OHTSUKI Takashi

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Enzymes which can degrade cell-wall polysaccharides of plants are useful for effective conversion of plant biomass. In this project, we focused on a parasitic plant, Orobanche minor, which can degrade the cell-wall of roots of their host plants. From RNAseq data, a beta-glucosidase homologous gene was obtained. The enzyme expressed in E. coli showed beta-mannosidase activity when cell-wall polysaccharides from O. minor seeds were used as a substrate. This is the first report that indicates beta-mannosidase from plants showed activity toward plant cell-wall components.
Analysis and Utilization of Function of Microbial Community

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Grant type：Competitive
Biological conversion of Waste Biomass to Valuable Products

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Grant type：Competitive
Biological Degradation of Artificial Chemicals

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Grant type：Competitive
Production of useful compounds

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Grant type：Competitive
Utilization of biomass resources

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Grant type：Competitive
Utilization of network in microbial community

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Grant type：Competitive
Utilization of various functions of microorganisms

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Grant type：Competitive
バイオマスの有効利用

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Grant type：Competitive
代謝工学･遺伝子工学手法を用いた有用物質生産

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Grant type：Competitive
微生物機能の高度利用

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Grant type：Competitive
複合微生物系構造・機能の解明と応用

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Grant type：Competitive

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Papers

A simple method for the preparation of single cells and regeneration of colonies of Botryococcus braunii NIES836 Reviewed Major achievement

Kengo Murayama, Takashi Ohtsuki

Journal of Microbiological Methods 216 106859 2023.11

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1016/j.mimet.2023.106859
世界に例をみない「刈草特化型」メタン発酵プロセスの開発をめざす Invited Major achievement

大槻隆司

アグリバイオ 7 ( 7 ) 627 - 629 2023.7

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Authorship：Lead author,　Last author,　Corresponding author Language：Japanese Publishing type：(MISC) Introduction and explanation (commerce magazine)
バイオマスは化石資源を代替できるか　〜バイオマス利用の現状と課題〜 Invited

大槻隆司

日本技術士会山梨県支部報 ( 11 ) 5 2023.1

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Authorship：Lead author,　Last author,　Corresponding author Language：Japanese Publishing type：(MISC) Introduction and explanation (others)
輸入依存からの脱却を目指して　薬用作物ミシマサイコの高効率発芽法の開発 Invited Major achievement

大槻隆司

農耕と園芸 77 ( 1 ) 30 - 33 2022.2

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Authorship：Lead author,　Last author,　Corresponding author Language：Japanese Publishing type：(MISC) Introduction and explanation (commerce magazine)
Effective methane production from the Japanese weed Gyougi-shiba (Cynodon dactylon) is accomplished by colonization of microbial communities that assimilate water-soluble and -insoluble fractions Reviewed Major achievement

Shuhei Matsuda, Takashi Ohtsuki

FEMS MICROBIOLOGY LETTERS 386 ( 4 ) fnab015 2021.2( ISSN：0378-1097 eISSN：1574-6968 )

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press

ABSTRACT

Weed, an abundant biomass, is considered unsuitable as a raw material for methane production. There are few reports on the anaerobic digestion of weeds without the addition of other organic wastes. To solve this problem, a methane-producing microbial community with weed as a sole feedstock was established. This study mainly focused on the degree of contribution between water-soluble and -insoluble fractions of the weed to methane production; thus, methane production from both fractions was tested separately. Methane production after 80-day batch cultures with whole weed, water-soluble and water-insoluble fractions was 184.5, 96.8 and 26.5 NmL g−1 dry matter (DM), respectively. The results of 16S rRNA gene amplicon sequence analysis revealed that Proteiniphilum saccharofermentans and several Methanobacterium species commonly dominated all cultures, whereas the population dynamics of minor species differed in every culture. Moreover, the remixed culture of microbial communities adapted to water-soluble and -insoluble fractions recovered methane production (252.4 NmL g−1 DM). Based on these results, it can be strongly inferred that colocalizing the minor species in water-soluble and -insoluble fractions is important for effective methane production.

DOI： 10.1093/femsle/fnab015

Other Link： http://academic.oup.com/femsle/article-pdf/368/4/fnab015/36450867/fnab015.pdf
Batch-mode analysis of thermophilic methanogenic microbial community changes in the overacidification stage in beverage waste treatment Reviewed Major achievement

Shuhei Matsuda, Takahiro Yamato, Yoshiyuki Mochizuki, Yoshinori Sekiguchi, Takashi Ohtsuki

International Journal of Environmental Research and Public Health 17 ( 20 ) 7514 - 7514 2020.10( ISSN：1661-7827 eISSN：1660-4601 )

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal) Publisher：MDPI AG

Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus, Defluviitoga tunisiensis, Acetomicrobium mobile, and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification.

DOI： 10.3390/ijerph17207514
薬用作物ミシマサイコの国内における高品質・高収量生産に向けた技術開発 Invited Major achievement

大槻隆司

ケミカル・エンジニヤリング 65 ( 7 ) 410 - 414 2020.7

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Authorship：Lead author,　Last author,　Corresponding author Language：Japanese Publishing type：(MISC) Introduction and explanation (commerce magazine) Publisher：化学工業社
Establishment and analysis of microbial communities capable of producing methane from grass waste at extremely high C/N ratio Reviewed Major achievement

Matsuda S, Ohtsuki T

International Journal of New Technology and Research 2 ( 9 ) 81 - 86 2016.9

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Authorship：Last author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)
Establishment and analysis of microbial communities capable of producing methane from grass waste at extremely high C/N ratio. Reviewed

Matsuda S, Ohtsuki T

International Journal of New Technology and Research 2 ( 9 ) 81 - 86 2016.9

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Language：English Publishing type：Research paper (scientific journal)
Ion detection by intramolecular charge-transfer absorption of benzocrown-bipyridinium conjugate appending octadecyl chain Reviewed

Kuwabara T, Satake R, Guo H, Katsumata M, Takahashi M, Suzuki Y, Ohtsuki T, Sato T, Kurokawa H

Journal of Ion Exchange 27 ( 2 ) 27 - 32 2016.5

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Language：English Publishing type：Research paper (scientific journal)
Ion detection by intramolecular charge-transfer absorption of benzocrown-bipyridinium conjugate appending octadecyl chain. Reviewed

Kuwabara T, Satake R, Guo H, Katsumata M, Takahashi M, Suzuki Y, Ohtsuki T, Sato T, Kurokawa H

Journal of Ion Exchange 27 ( 2 ) 27 - 32 2016.5

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.5182/jaie.27.27
Acceleration of removal of high concentration-phenol by activated sludge with nitrate-oxidized lignite Reviewed Major achievement

Ohtsuki T, Hanagata M, Masuda T, Ui S

Japanese Journal of Water Treatment Biology 51 ( 2 ) 37 - 47 2015.6

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Authorship：Lead author,　Corresponding author Language：English Publishing type：Research paper (scientific journal)
Acceleration of removal of high concentration-phenol by activated sludge with nitrate-oxidized lignite. Reviewed

Ohtsuki T, Hanagata M, Masuda T, Ui S

Japanese Journal of Water Treatment Biology 51 ( 2 ) 37 - 47 2015.6( ISSN：0910-6758 )

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Language：English Publishing type：Research paper (scientific journal)

DOI： 10.2521/jswtb.51.37

CiNii Books
Modification of chimeric (2S,3S)-butanediol dehydrogenase based on structural information Reviewed Major achievement

Shimegi T, Mochizuki K, Ooyama T, Ohtsuki T, Kusunoki M, Ui S

Protein and Peptide Letters 22 ( 3 ) 226 - 233 2015.3

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Language：English Publishing type：Research paper (scientific journal)
Modification of Chimeric (2S, 3S)-butanediol Dehydrogenase Based on Structural Information Reviewed

Tomohito Shimegi, Kaito Mochizuki, Takuji Oyama, Takashi Ohtsuki, Masami Kusunoki, Sadaharu Ui

PROTEIN AND PEPTIDE LETTERS 22 ( 3 ) 226 - 233 2015( ISSN：0929-8665 eISSN：1875-5305 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：BENTHAM SCIENCE PUBL LTD

A chimeric (2S, 3S)-butanediol dehydrogenase (cLBDH) was engineered to have the strict (S)-configuration specificity of the (2S, 3S)-BDH (BsLBDH) derived from Brevibacterium saccharolyticum as well as the enzymatic stability of the (2R, 3S)-BDH (KpMBDH) from Klebsiella pneumonia by swapping the domains of two native BDHs. However, while cLBDH possesses the stability, it lacks the specificity. In order to assist in the design a BDH having strict substrate specificity, an X-ray structural analysis of a cLBDH crystal was conducted at 1.58 angstrom. The results obtained show some readily apparent differences around the active sites of cLBDH and BsLBDH. Based on this structural information, a novel (2S, 3S)-BDH having a preferred specificity was developed by introducing a V254L mutation into cLBDH. The influence of this mutation on the stability of cLBDH was not evaluated. Nevertheless, the technique described herein is an effective method for the production of a tailor-made BDH.

Web of Science
Streptomyces hokutonensis sp. nov., a novel actinomycete isolated from the strawberry root rhizosphere Reviewed

Hideki Yamamura, Haruna Ashizawa, Moriyuki Hamada, Akira Hosoyama, Hisayuki Komaki, Misa Otoguro, Tomohiko Tamura, Yukikazu Hayashi, Youji Nakagawa, Takashi Ohtsuki, Nobuyuki Fujita, Sadaharu Ui, Masayuki Hayakawa

JOURNAL OF ANTIBIOTICS 67 ( 6 ) 465 - 470 2014.6( ISSN：0021-8820 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：JAPAN ANTIBIOTICS RESEARCH ASSOC

A polyphasic approach was used to determine the taxonomic position of actinomycete strain R1-NS-10(T), which was isolated from a sample of strawberry root rhizosphere obtained from Hokuto, Yamanashi, Japan. Strain R1-NS-10(T) was Gram-staining-positive and aerobic, and formed brownish-white aerial mycelia and grayish-brown substrate mycelia on ISP-2 medium. The strain grew in the presence of 0-5% (w/v) NaCl and optimally grew without NaCl. The strain grew at pH 5-8, and the optimum for growth was pH 7. The optimal growth temperature was 30 degrees C, but the strain grew at 5-37 degrees C. Whole-cell hydrolysates of strain R1-NS-10(T) contained A(2)pm, galactose, mannose and rhamnose. The predominant menaquinones were MK-9(H-6) and MK-9(H-8). The major cellular fatty acids were anteiso-C-15:0 and iso-C-16:0. Comparative 16S rRNA gene sequence analysis revealed that strain R1-NS-10(T) was most closely related to Streptomyces prunicolor NBRC 13075(T) (99.4%). The draft genome sequences of both strains were determined for characterization of genome sequence-related parameters such as average nucleotide identity (ANI) and the diversity of secondary metabolite biosynthetic gene clusters. DNA-DNA hybridization (DDH) and ANI values for both strains were below the species delineation cutoff, and differences in physiological and biochemical characteristics differentiated strain R1-NS-10T from its closest phylogenetic relative. On the basis of these data, we propose that strain R1-NS-10(T) (= NBRC 108812(T) = KCTC 29186(T)) should be classified as the type strain of a novel Streptomyces species named Streptomyces hokutonensis sp. nov.

DOI： 10.1038/ja.2014.20

Web of Science
Streptomyces hokutoensis sp. Nov., a novel actinomycete isolated from the strawberry root rhizosphere Reviewed Major achievement

Yamamura H, Ashizawa H, Hamada M, Hosoyama A, Komaki H, Otoguro M, Tamura T, Hayashi Y, Nakagawa Y, Ohtsuki T, Fujita N, Ui S, Hayakawa M

The Journal of Antibiotics 67 ( 6 ) 465 - 470 2014.4

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Language：English Publishing type：Research paper (scientific journal)
Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol dehydrogenase Reviewed Major achievement

Shimegi T, Ooyama T, Ohtsuki T, Kurisu G, Kusunoki M, Ui S

Acta Crystallographica Section F, Structural Biology Communications 70 ( 4 ) 461 - 463 2014.4

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Language：English Publishing type：Research paper (scientific journal)
Crystallization and preliminary X-ray diffraction analysis of domain-chimeric L-(2S,3S)-butanediol dehydrogenase Reviewed

Tomohito Shimegi, Takuji Ooyama, Takashi Ohtsuki, Genji Kurisu, Masami Kusunoki, Sadaharu Ui

ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS 70 ( 4 ) 461 - 463 2014.4( ISSN：1744-3091 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

A domain-chimeric L-2,3-butanediol dehydrogenase (chimera L-BDH), which was designed to possess both the S-configuration specificity of L-BDH and the stability of meso-BDH, was constructed by exchanging the respective domains of these two BDHs. However, chimera L-BDH possessed a lower enzymatic function than expected based on the two original enzymes. To elucidate the causes of the decreased stability and substrate specificity, crystallization of the protein was performed. Chimera L-BDH was purified to homogeneity via ammonium sulfate fractionation and three column-chromatography steps, and was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group C222(1), diffracted synchrotron radiation to 1.58 angstrom resolution and were most likely to contain two molecules in the asymmetric unit.

DOI： 10.1107/S2053230X13032755

Web of Science
2P-127 Isolation of the commensal bacterium enhancing growth and oil production of Botryococcus braunii

Ohtsuki Takashi, Sugauchi Yuichiro, Ui Sadaharu

66 138 - 138 2014

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Language：Japanese Publishing type：(MISC) Summary of the papers read (national conference and other science council)

CiNii Books
Crystallization and preliminary X-ray diffraction analysis of domain-chimeric l-(2S,3S)-butanediol dehydrogenase Reviewed

Tomohito Shimegi, Takuji Ooyama, Takashi Ohtsuki, Genji Kurisu, Masami Kusunoki, Sadaharu Ui

Acta Crystallographica Section F:Structural Biology Communications 70 ( 4 ) 461 - 463 2014( ISSN：2053-230X )

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Language：English Publishing type：Research paper (scientific journal) Publisher：International Union of Crystallography

A domain-chimeric l-2,3-butanediol dehydrogenase (chimera l-BDH), which was designed to possess both the S-configuration specificity of l-BDH and the stability of meso-BDH, was constructed by exchanging the respective domains of these two BDHs. However, chimera l-BDH possessed a lower enzymatic function than expected based on the two original enzymes. To elucidate the causes of the decreased stability and substrate specificity, crystallization of the protein was performed. Chimera l-BDH was purified to homogeneity via ammonium sulfate fractionation and three column-chromatography steps, and was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group C2221, diffracted synchrotron radiation to 1.58 Å resolution and were most likely to contain two molecules in the asymmetric unit. © 2014 International Union of Crystallography All rights reserved.

DOI： 10.1107/S2053230X13032755

Scopus

PubMed
3P-174 Progression of wood biodegradability for hydrogen-/methane fermentation by retreatment of mushroom culture substrate with edible mushrooms

Osada Moyuru, Ohtsuki Takashi, Ui Sadaharu

66 238 - 238 2014

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Language：Japanese Publishing type：(MISC) Summary of the papers read (national conference and other science council)

CiNii Books
3P-172 Agrobacterium tumefaciens-mediated transformation of white-rot fungus Ceriporiopsis subvermispora

Suzuki Ami, Ohtsuki Takashi, Ui Sadaharu

66 237 - 237 2014

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Language：Japanese Publishing type：(MISC) Summary of the papers read (national conference and other science council)

CiNii Books
X-ray crystallographic analysis of domain-chimeric L-(2S,3S)-butanediol dehydroggenase

T. SHimegi, T. Ooyama, T. Ohtsuki, M. Kusunoki, S. Ui

Photon Factory Activity Report 2013 #31B 2013

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Language：English Publishing type：Research paper (bulletin of university, research institution)
バイオマスの有効利用を妨げる渋滞問題 Invited

大槻隆司

日本生物工学会誌 90 ( 2 ) 93 - 93 2012( ISSN：0919-3758 )

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Language：Japanese Publishing type：(MISC) Introduction and explanation (scientific journal) Publisher：日本生物工学会

CiNii Books

Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=304725
Chitin nanowhiskers mediate transformation of Escherichia coli by exogenous plasmid DNA Reviewed Major achievement

Mera A, Araki J, Ohtsuki T, Shimosaka M, Yoshida N

Journal of Biotechnology and Biomaterials 1 ( 6 ) 114 2011.9

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Language：English Publishing type：Research paper (scientific journal)
Construction of a tailor-made L(2S,3S)-butanediol dehydrogenase by exchanging domains between native structural analogs Reviewed Major achievement

Shimegi T, Takusagawa Y, Ohtsuki T, Noda S, Kurisu G, Kusunoki M, Ui S

Protein and Peptide Letters 18 825 - 830 2011.8

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Language：English Publishing type：Research paper (scientific journal)
Construction of a Tailor-Made L (2S, 3S)-Butanediol Dehydrogenase by Exchanging Domains Between Native Structural Analogs Reviewed

Tomohito Shimegi, Yuhsuke Takusagawa, Takashi Ohtsuki, Satoko Noda, Genji Kurisu, Masami Kusunoki, Sadaharu Ui

PROTEIN AND PEPTIDE LETTERS 18 ( 8 ) 825 - 830 2011.8( ISSN：0929-8665 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：BENTHAM SCIENCE PUBL LTD

The development of a stable L-BDH chimera was attempted by exchanging whole domains between two native structural analogs, L-BDH and meso-BDH, because the S-configuration specificity of L-BDH is valuable from the standpoint of its application but its activity is unstable, whereas meso-BDH is stable. The domain chimeras obtained indicated that the leaf-like structures constituting three domains were likely to be mainly associated with chiral recognition, and the fourth domain, the basic domain, is likely to be mainly associated with enzyme stability. A combination of the leaf domains of L-BDH and the basic domain of meso-BDH attained a sufficient level of practical use as an artificial L-BDH chimera, because the resulting enzyme had both stability and S-configuration specificity. However, the levels of stability and specificity were slightly lower than those of the respective enzymes from which they were derived.

Web of Science
Improvement of bioconversion suitability of Japanese cypress wood by combination of UV radiation, ozonization and decay treatment with white-rot and brown-rot fungi Reviewed Major achievement

Ohtsuki T, Noda S, Ui S

Canadian Journal of Pure and Applied Sciences 5 ( 1 ) 1333 - 1343 2011.2

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Chitin nanowhiskers mediate transformation of Escherichia coli by exogenous plasmid DNA Reviewed

Mera A, Araki J, Ohtsuki T

Journal of Biotechnology and Biomaterials 1 ( 6 ) 114 2011

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Language：English Publishing type：Research paper (scientific journal)
Improvemnet of bioconversion suitability of Japanese cypress wood by combination of UV radiation, ozonization and decay treatment with white-rot and brown-rot fungi Reviewed

Ohtsuki T, Noda S, Ui S

Canadian Journal of Pure and Applied Sciences 5 ( 1 ) 1333 - 1343 2011

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Language：English Publishing type：Research paper (scientific journal)
Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases Reviewed Major achievement

Otagiri M, Ui S, Takusagawa Y, Ohtsuki T, Kurisu G, Kusunoki M

FEBS Letters 584 ( 1 ) 219 - 223 2010.1

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Language：English Publishing type：Research paper (scientific journal)
Structural basis for chiral substrate recognition by two 2,3-butanediol dehydrogenases Reviewed

Masato Otagiri, Sadaharu Ui, Yuhsuke Takusagawa, Takashi Ohtsuki, Genji Kurisu, Masami Kusunoki

FEBS LETTERS 584 ( 1 ) 219 - 223 2010.1( ISSN：0014-5793 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

2,3-Butanediol dehydrogenase (BDH) catalyzes the NAD-dependent redox reaction between acetoin and 2,3-butanediol. There are three types of homologous BDH, each stereospecific for both substrate and product. To establish how these homologous enzymes possess differential stereospecificities, we determined the crystal structure of L-BDH with a bound inhibitor at 2.0 angstrom. Comparison with the inhibitor binding mode of meso-BDH highlights the role of a hydrogen-bond from a conserved Trp residue(192). Site-directed mutagenesis of three active site residues of meso-BDH, including Trp(190), which corresponds to Trp(192) of L-BDH, converted its stereospecificity to that of L-BDH. This result confirms the importance of conserved residues in modifying the stereospeci. city of homologous enzymes. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

DOI： 10.1016/j.febslet.2009.11.068

Web of Science

PubMed
ブランチスピリット・東日本支部の生物工学教育活動(1) 〜学生発表討論会〜 Invited

大槻隆司

日本生物工学会誌 88 ( 1 ) 28 - 29 2010( ISSN：0919-3758 )

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Language：Japanese Publishing type：(MISC) Other article Publisher：日本生物工学会

CiNii Books

Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=303835
Development of activated sludge adapted to high concentration of phenol and enhancement of its phenol removal ability by addition of a processed lignite Reviewed Major achievement

Ohtsuki T, Sakurai K, Noda S, Ichihara J, Masuda T, Ui S

Indian Journal of Science and Technology 2 ( 10 ) 1 - 4 2009.10

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
High-yield Production of Scutellaria Radix Flavonoids (Baicalein, Baicalin and Wogonin) by Liquid-culture of Scutellaria baicalensis Root-derived Cells Reviewed

Takashi Ohtsuki, Mika Himeji, Harumi Fukazawa, Miho Tanaka, Hisako Yamamoto, Akio Mimura

BRAZILIAN ARCHIVES OF BIOLOGY AND TECHNOLOGY 52 ( 2 ) 291 - 298 2009.3( ISSN：1516-8913 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：INST TECNOLOGIA PARANA

Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the extremely high production rate (339 mg/L.week) was obtained. In the comparison of total antioxidative activities among baicalein, baicalin and wogonin, evaluated by thiocyanate method, it was suggested that the location of hydroxyl groups both at 5- and 6-position contributed to enhancement of radical scavenging activity, and/or methoxylation at 8-position diminished the activity. The possibility of utilizing these flavonoids for natural antioxidants and medicine is also discussed.

DOI： 10.1590/S1516-89132009000200005

Web of Science
High-yield production of Scutellaria Radix flavonoids (baicalein, baicalin and wogonin) by liquid-culture of Scutellaria baicalensis-derived cells Reviewed Major achievement

Ohtsuki T, Himeji M, Fukazawa H, Tanaka M, Yamamoto H, Mimura A

Brazilian Archives of Biology and Technology 52 ( 2 ) 291 - 298 2009.1

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Development of activated sludge adapted to high concentrations of phenol and enhancement of its phenol removal ability by addition of a processed lignite Reviewed

Takashi Ohtsuki, Kazuki Sakurai, Satoko Noda, Joji Ichihara, Takahito Masuda, Sadaharu Ui

Indian Journal of Science and Technology 2 ( 10 ) 1 - 4 2009( ISSN：0974-5645 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：Indian Society for Education and Environment

In this study, we successfully developed activated sludge (AS) adapted to a very high concentration (3 g/L) of phenol. A careful stepwiseadaptation process was considered important in the development of this AS. In addition, we found that processed lignite (PL) accelerated the removal of phenol which might lead to change in the dominant members of the microbial community. Phenol is an abundant industrial toxic contaminant in wastewater treatment processes.

DOI： 10.17485/ijst/2009/v2i10/30708

Scopus
水素利用インフラ整備は微生物界でも大変!? Invited

大槻隆司

日本生物工学会誌 87 ( 3 ) 135 - 135 2009( ISSN：0919-3758 )

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Language：Japanese Publishing type：(MISC) Introduction and explanation (scientific journal) Publisher：日本生物工学会

CiNii Books

Other Link： https://projects.repo.nii.ac.jp/?action=repository_uri&item_id=303489
Difference of growth-inhibitory effect of Scutellaria baicalensis-producing flavonoid wogonin among human cancer cells and normal diploid cell Reviewed Major achievement

Himeji M, Ohtsuki T, Fukazawa H, Tanaka M, Yazaki S, Ui S, Nishio K, Yamamoto H, Tasaka K, Mimura A

Cancer Letters 245 ( 1-2 ) 269 - 274 2007.1

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Difference of growth-inhibitory effect of Scutellaria baicalensis-producing flavonoid wogonin among human cancer cells and normal diploid cell Reviewed

Mika Himeji, Takashi Ohtsuki, Harumi Fukazawa, Miho Tanaka, Shin-ichi Yazaki, Sadaharu Ui, Koji Nishio, Hisako Yamamoto, Kachio Tasaka, Akio Mimura

CANCER LETTERS 245 ( 1-2 ) 269 - 274 2007.1( ISSN：0304-3835 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER IRELAND LTD

Methanol extract from cultured Scutellaria baicalensis cells inhibited the proliferation of human monocytic leukemia cell line THP-1 and human osteogenic sarcoma cell line HOS. The inhibitory effects of baicalin, baicalein and wogonin, the three major flavonoids contained in the extract, were studied. It should be noted that wogonin did not show the inhibitory effect on human fetal lung normal diploid cell line TIG-1, as compared to the inhibition observed in cancer cells. Physiological analyses in THP-1 cells showed that wogonin induced cell cycle arrest at G(2)/M phase and apoptosis. This is the first report discovering a cancer-specific apoptosis-inducing activity of wogonin. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.canlet.2006.01.011

Web of Science
”自由研究は面白い”に誘う私の工夫ー生徒の興味との接点を探すー Invited

大槻隆司

楽しい理科授業 38 ( 7 ) 7 2006

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Language：Japanese Publishing type：(MISC) Introduction and explanation (others)
Production of large multienzyme complex by aerobic thermophilic fungus Chaetomium sp. nov. MS-017 grown on palm oil mill fibre Reviewed Major achievement

Ohtsuki T, Suyanto, Yazaki S, Ui S, Mimura A

Letters in Applied Microbiology 40 ( 2 ) 111 - 116 2005.1

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Production of large multienzyme complex by aerobic thermophilic fungus Chaetomium sp nov MS-017 grown on palm oil mill fibre Reviewed

T Ohtsuki, Suyanto, S Yazaki, S Ui, A Mimura

LETTERS IN APPLIED MICROBIOLOGY 40 ( 2 ) 111 - 116 2005( ISSN：0266-8254 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL PUBLISHING LTD

Aims: A novel xylanolytic multienzyme complex of the aerobic thermophilic fungus Chaetomium sp. nov. MS-017 was produced on palm oil mill fibre (POMF) and partially characterized.
Methods and Results: The assay of the extracellular enzymes of Chaetomium sp. nov. MS-017 on POMF in solid-state fermentation revealed cellulolytic, pectinolytic and extremely high xylanolytic activities. The protein was purified by Sephadex G-200 column chromatography. The SDS-PAGE demonstrated that the purified protein is a complex with at least five xylanolytic, four cellulolytic and eight pectinolytic components. The characterization of the complex at various temperatures showed that the reactivity and stability of the complex are not lost up to 60degreesC. In addition, the complex was very stable in a wide range of pH (3-9) and at high concentrations (10 mM) of cations and EDTA. The major products of xylan hydrolysis by the purified complex were determined to be xylobiose and xylotriose by thin-layer chromatography.
Conclusion: Chaetomium sp. nov. MS-017 preferentially produces a xylanolytic multienzyme complex on POMF in solid-state fermentation.
Significance and Impact of the Study: This is the first report on the xylanolytic multienzyme complex produced by an aerobic thermophilic fungus.

DOI： 10.1111/j.1472-765X.2004.01644.x

Web of Science

PubMed
Induction of apoptosis in human leukemia cells by naturally fermented sugar cane vinegar (kibizu) of Amami Ohshima Island Reviewed Major achievement

Mimura A, Suzuki Y, Toshima Y, Yazaki S, Ohtsuki T, Ui S, Hyodoh F

BioFactors 22 ( 1-4 ) 93 - 97 2004.12

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Production of L-2,3-butanediol by a new pathway constructed in Escherichia coli Reviewed Major achievement

Ui S, Takusagawa Y, Sato T, Ohtsuki T, Mimura A, Ohkuma M, Kudo T

Letters in Applied Microbiology 39 ( 6 ) 533 - 537 2004.11

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2G11-5 Development of biological control system for repressing basal stem cell rotting mushroom Ganoderma spp. on oil palm trees

NAKANO Masako, AKIZUMI Hironobu, AHKAM Subroto, YAZAKI Shinichi, OHTSUKI Takashi, UI Sadaharu, MIMURA Akio

16 200 - 200 2004

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Induction of apoptosis in human leukemia cells by naturally fermented sugar cane vinegar (kibizu) of Amami Ohshima Island Reviewed

A Mimura, Y Suzuki, Y Toshima, S Yazaki, T Ohtsuki, S Ui, F Hyodoh

BIOFACTORS 22 ( 1-4 ) 93 - 97 2004( ISSN：0951-6433 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

Naturally fermented vinegar such as Kibizu (sugar cane vinegar in Amami Ohshima, Japan), Kurozu (black rice vinegar in Kagoshima. Japan), Kouzu (black rice vinegar in China) and red wine vinegar in Italy had potent radical-scavenging activity analyzed by DPPH method. For the elucidation of food factor for cancer prevention contained in naturally fermented vinegar. the induction of apoptosis in human leukemia cell HL-60 was investigated with sugar cane vinegar Kibizu.
Fraction eluted by 40% methanol from Amberlite XAD 2 chromatography of sugar cane vinegar showed potent radical scavenging activity. The fraction also showed the activity repressing growth of typical human leukemia cells such as HL-60, THP-1 Molt-4, U-937, Jurkat, Raji and K-562. On the other hand, the fraction did not have any growth inhibition activity against human fetal lung cell TIG-1. The most potent radical-scavenging activity and the growth repression activity of the leukemia cell were observed in the same chromatographic fraction of methanol 40%.
From cell sorting FACS analyses, electron microscopic observations and cytochemical staining of chromatin and nuclear segments in human leukemia cell HL-60 treated with the active fraction, it was concluded that apoptosis was induced in the leukemia cell by the fraction of sugar cane vinegar and resulted in the repression of growth of the human leukemia cells.
Chromatographic fraction of sugar cane juice eluted by 20% methanol showed potent activities of radical-scavenging and growth repression of HL-60. These results led us the consideration that active components in sugar cane juice could be converted to more lipophilic compounds with activity to induce apoptosis in HL-60 by microbial fermentation with yeast and acetic acid bacteria.

Web of Science
Production of L-2,3-butanediol by a new pathway constructed in Escherichia coli Reviewed

S Ui, Y Takusagawa, T Sato, T Ohtsuki, A Mimura, M Ohkuma, T Kudo

LETTERS IN APPLIED MICROBIOLOGY 39 ( 6 ) 533 - 537 2004( ISSN：0266-8254 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：BLACKWELL PUBLISHING LTD

Aims: A metabolic pathway for L-2,3-butanediol ( BD) as the main product has not yet been found. To rectify this situation, we attempted to produce L-BD from diacetyl (DA) by producing simultaneous expression of diacetyl reductase (DAR) and L-2,3-butanediol dehydrogenase (BDH) using transgenic bacteria, Escherichia coli JM109/ pBUD-comb.
Methods and Results: The meso-BDH of Klebsiella pneumoniae was used for its DAR activity to convert DA to L-acetoin (AC) and the L-BDH of Brevibacterium saccharolyticum was used to reduce L-AC to L-BD. The respective gene coding each enzyme was connected in tandem to the MCS of pFLAG-CTC (pBUD-comb). The divided addition of DA as a source, addition of 2% glucose, and the combination of static and shaking culture was effective for the production.
Conclusions: L-BD ( 2200 mg l(-1)) was generated from 3000 mg l(-1) added of DA, which corresponded to a 73% conversion rate. Meso-BD as a by-product was mixed by 2% at most.
Significance and Impact of the Study: An enzyme system for converting DA to L- BD was constructed with a view to using DA-producing bacteria in the future.

DOI： 10.1111/j.1472-765X.2004.01622.x

Web of Science

PubMed
Increased production of antioxidative sesaminol glucosides from sesame oil cake through fermentation by Bacillus circulans strain YUS-2 Reviewed Major achievement

Ohtsuki T, Akiyama J, Shimoyama T, Yazaki S, Ui S, Hirose Y, Mimura A

Bioscience, Biotechnology, and Biochemistry 67 ( 10 ) 2304 - 2306 2003.10

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Increased production of antioxidative sesaminol glucosides from sesame oil cake through fermentation by Bacillus circulans strain YUS-2 Reviewed

T Ohtsuki, J Akiyama, T Shimoyama, S Yazaki, S Ui, Y Hirose, A Mimura

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 67 ( 10 ) 2304 - 2306 2003.10( ISSN：0916-8451 eISSN：1347-6947 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD

Bacillus circulans strain YUS-2 was isolated as the strongest antioxidant-producer in fermentation of sesame oil cake (SOC, defatted residue yielded from sesame seed oil production). Two major strong antioxidants from fermented SOC were purified and identified as known sesaminol triglucoside and sesaminol diglucoside, however, our results demonstrated that the fermentation process with B. circulans YUS-2 was highly effective to gain the extraction efficiency of the sesaminol glucosides.

DOI： 10.1271/bbb.67.2304

Web of Science

PubMed
Possibility of efficient composting on palm oil mill fiber by optimized solid state fermentation using thermophilic fungus Chaetomium sp. nov. MS-017 Reviewed Major achievement

Suyanto, Ohtsuki T, Yazaki S, Ui S, Subroto MA, Koesnandar, Mimura A

Jurnal Mikrobiologi Indonesia (Indonesian Journal for Microbiology) 8 ( 2 ) 57 - 62 2003.9

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Isolation of a novel thermophilic fungus Chaetomium sp. nov. MS-017 and description of its palm oil mill fiber-decomposing properties Reviewed Major achievement

Suyanto, Ohtsuki T, Yazaki S, Ui S, Mimura A

Applied Microbiology and Biotechnology 60 ( 5 ) 581 - 587 2003.1

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Isolation of a novel thermophilic fungus Chaetomium sp nov MS-017 and description of its palm-oil mill fiber-decomposing properties Reviewed

Suyanto, T Ohtsuki, S Yazaki, S Ui, A Mimura

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY 60 ( 5 ) 581 - 587 2003.1( ISSN：0175-7598 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：SPRINGER-VERLAG

Palm-oil mill fiber (POMF) is a fibrous, natural hard material discharged in enormous amounts from palm-oil mills in tropical plantations: therefore, research to find microorganisms that decompose POMF was conducted. As the result of screening, a new thermophilic fungus, Chaetomium sp. nov. MS-017, exhibiting rapid growth on POMF was isolated from rotted wood. Based on partial characterization of the decomposition of POMF, it was shown that MS-017 preferentially assimilates poly saccharides, especially hemicelluloses such as xylan. A preliminary composting study indicated that MS-017 produced 855 g of decomposed product from 1,000 g of intact POMF in 12 days under optimized solid-culture conditions. The decomposition rate of POMF was 23% (w/w), and the cell yield calculated from consumed POMF was as high as 36% (w/w). These results indicate that MS-017 has a very high potential to decompose POMF and that it is suitable for economical production of compost to recycle by-product biomass from oil-palm plantations.

DOI： 10.1007/s00253-002-1125-2

Web of Science
Characterization of xylanosome produced by Chaetomium sp. nov. MS-017 on solid state fermentation of palm oil mill fiber

Ohtsuki Takashi, Suyanto, Yazaki Shinichi, Ui Sadaharu, Mimura Akio

15 113 - 113 2003

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3K10-1 Utilization of palm oil mill fiber in situ bioactivator process by Chaetomium sp. nov. MS-017 :

Suyanto, Ohtsuki Takashi, Yazaki Shinichi, Ui Sadaharu, Mimura Akio

15 227 - 227 2003

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CiNii Books
Possibility of efficient composting on palm oil mill fiber by optimized solid state fermentation using thermophilic fungus Chaetomium sp. nov. MS-017 Reviewed

Suyanto,Takashi Ohtsuki

Jurnal Mikrobiologi Indonesia (Indonesian Journal for Microbiology) 8 ( 2 ) 57 - 62 2003

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Language：English Publishing type：Research paper (scientific journal)
ゴマ脱脂粕に含まれるセサミノール配糖体の微生物発酵による遊離促進効果

大槻隆司, 三村精男

Sesame Newsletter ( 17 ) 4 - 5 2003

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Acetylacetoin synthase as a marker enzyme for detecting the 2,3-butanediol cycle Reviewed Major achievement

Ui S, Hosaka T, Mizutani K, Ohtsuki T, Mimura A

Journal of Bioscience and Bioengineering 93 ( 2 ) 248 - 251 2002.2

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Acetylacetoin synthase as a marker enzyme for detecting the 2,3-butanediol cycle Reviewed

S Ui, T Hosaka, K Mizutani, T Ohtsuki, A Mimura

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 93 ( 2 ) 248 - 251 2002.2( ISSN：1389-1723 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN

Acetylacetoin synthase (AACSase) and acetylacetoin reductase (AACRase) are representative enzymes of the 2,3-butanediol cycle. After examining their induction conditions in various bacteria, the former was induced by acetoin and the latter by glucose. All strains carrying AACSase also had AACRase, but the reverse was not true. Therefore, AACSase indicates the existence of the cycle. Acetylacetoin (AAC) accumulation or the ratio of 2,3-butanediol isomer formed also indicated the presence of the cycle in bacteria. This cycle is present in some strains and not in others even for those belonging to the same species. The cycle was not always associated with the representative 2,3-butanediol-producing bacteria or bacterial sporogenesis as reported previously.

DOI： 10.1263/jbb.93.248

Web of Science
Purification and characterization of L-2,3-butanediol dehydrogenase of Brevibacterium saccharolyticum C-1012 expressed in Escherichia coli Reviewed Major achievement

Takusagawa Y, Otagiri M, Ui S, Ohtsuki T, Mimura A, Ohkuma M, Kudo T

Bioscience, Biotechnology and Biochemistry 65 ( 8 ) 1876 - 1878 2001.8

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Purification and characterization of L-2,3-butanediol dehydrogenase of Brevibacterium saccharolyticum C-1012 expressed in Escherichia coli Reviewed

Y Takusagawa, M Otagiri, S Ui, T Ohtsuki, A Mimura, M Ohkuma, T Kudo

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 65 ( 8 ) 1876 - 1878 2001.8( ISSN：0916-8451 eISSN：1347-6947 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD

The L-2,3-butanediol dehydrogenase produced in E coti JM109/pLBD2-CTC was purified by 5 steps. The molecular mass of this enzyme was estimated at 110 kDa and the subunit was measured to be 30 kDa. The L-BDH had some differences from the BDHs from other sources in substrate specificity, pI value, pH stability, effects of divalent cations, and organic acids.

DOI： 10.1271/bbb.65.1876

Web of Science

PubMed
Characterization of the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase gene from Bacillus cereus YUF-4 Reviewed Major achievement

Hosaka T, Ui S, Ohtsuki T, Mimura A, Ohkuma M, Kudo T

Journal of Bioscience and Bioengineering 91 ( 6 ) 539 - 544 2001.6

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Characterization of the NADH-Linked acetylacetoin reductase/2,3-butanediol dehydrogenase gene from Bacillus cereus YUF-4 Reviewed

T Hosaka, S Ui, T Ohtsuki, A Mimura, M Ohkuma

JOURNAL OF BIOSCIENCE AND BIOENGINEERING 91 ( 6 ) 539 - 544 2001.6( ISSN：1389-1723 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN

A 1.4-kbp DNA fragment, including the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase (AACRII/BDH) gene from the chromosomal DNA of Bacillus cereus YUF-4, was cloned in Escherichia coli DH5 alpha after its insertion into pUC119, and the resulting plasmid was named pAACRII119. The AACRII/BDH gene had an open reading frame consisting of 1047 bp encoding 349 amino acids. The enzyme exhibited not only AACR activity, but also BDH activity. However, the gene was not located in a 2,3-butanediol (BD) operon, as is the case in the BDH gene of Klebsiella pneumoniae and that of K. terrigena. In addition, there was no BD-cycle-related enzyme gene in the region surrounding the AACRII/BDH gene. The AACR and BDH activities in E. coli DH5 alpha /pAACRII119 were 200-fold higher than those in the original B. cereus YUF-4. The characteristics of the AACRII/BDH from E. coli DH 5 alpha /pAACRII119 are similar to those of the AACRH/BDH from B. cereus YUF-4. The AACRH/BDH was considered to belong to the NAD(P)- and zinc-dependent long-chain alcohol dehydrogenase (group I ADH) family on the basis of the following distinctive characteristics: it possessed 14 strictly conserved residues of microbial group I ADH and consisted of about 350 amino acids. The enzymatic and genetic characteristics of AACRII/BDH were completely different from those of BDHs belonging to the short-chain dehydrogenase/reductase family. These findings indicated that the AACRII/BDH could be considered a new type of BDH.

DOI： 10.1263/jbb.91.539

Web of Science
Stereochemical applications of the expression of the L-2,3 butanediol dehydrogenase gene in Escherichia coli Reviewed Major achievement

Ui S, Takusagawa Y, Ohtsuki T, Mimura A, Ohkuma M, Kudo T

Letters in Applied Microbiology 32 ( 2 ) 93 - 98 2001.2

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Stereochemical applications of the expression of the L-2,3-butanediol dehydrogenase gene in Escherichia coli Reviewed

S Ui, Y Takusagawa, T Ohtsuki, A Mimura, M Ohkuma, T Kudo

LETTERS IN APPLIED MICROBIOLOGY 32 ( 2 ) 93 - 98 2001.2( ISSN：0266-8254 eISSN：1472-765X )

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Language：English Publishing type：Research paper (scientific journal) Publisher：WILEY-BLACKWELL

The L-2,3-butanediol dehydrogenase (L-BDH) gene of Brevibacterium saccharolyticum was strongly expressed in Escherichia coli using the tac promoter. However, the stereospecificity of the resulting L-BDH was reduced. The region upstream of the meso-BDH gene of Klebsiella pneumoniae was also involved in the expression of the B. saccharolyticum gene. However, in this case, the resulting L-BDH exhibited more stable stereospecificity. A stereospecificity recognition region was located within the rear sequence (Hpa I site, carboxy terminal) of the BDH open reading frame. Using a transformed strain of E. coli, the conversion of L-acetoin (L-AC), in the commercially available racemic mixture of AC, to L-2,3-butanediol (L-BD) was attempted. As a result, 0.37% L-BD was formed from 1% AC added to the culture.

DOI： 10.1046/j.1472-765X.2001.00869.x

Web of Science

PubMed
314 Enzymes decomposing palm oil mill fiber in fungus Ms-017 under higher temperature :

Suyanto, Yazaki Shin-ichi, Ohtsuki Takashi, Ui Sadaharu, Mimura Akio

13 168 - 168 2001

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Enzyme production from rice straw using fungus under higher temperature.

Ohtsuki Takashi, Suyanto, Yazaki Shin-ichi, Ui Sadaharu, Mimura Akio

13 168 - 168 2001

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Good or evil: CD26 and HIV infection Reviewed Major achievement

Ohtsuki T, Tsuda H, Morimoto C

Journal of Dermatological Science 22 ( 3 ) 152 - 160 2000.4

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Authorship：Lead author Language：English Publishing type：(MISC) Introduction and explanation (scientific journal)
Good or evil: CD26 and HIV infection Invited

T Ohtsuki, H Tsuda, C Morimoto

JOURNAL OF DERMATOLOGICAL SCIENCE 22 ( 3 ) 152 - 160 2000.4( ISSN：0923-1811 )

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Authorship：Lead author Language：English Publishing type：(MISC) Introduction and explanation (scientific journal) Publisher：ELSEVIER SCI IRELAND LTD

Acquired immune deficiency syndrome (AIDS:) is an incurable disease at present and so many efforts to conquer this disease are being made around the world. In studies of human immunodeficiency virus (HIV) infection and the disease progression. it has been reported that T cells expressing CD26 are preferentially infected and depleted in HIV-infected individuals. CD26 is a widely distributed 110 kDa cell-surface glycoprotein with known dipeptidyl peptidase IV (DPPIV) activity in its extracellular domain. This ectoenzyme is capable of cleaving N-terminal dipeptides from polypeptides with either proline or alanine residues in the penultimate position. On human T cells. CD26 exhibits the co-stimulatory function and plays an important role in immune response via its ability to bind adenosine deaminase (ADA) and association with CD45. Recent studies have been stripping the veil from over the relationship between CD26 and HIV infection. Susceptibility of cells to HIV infection is correlated with CD26 expression. and HIV transactivator Tat and envelope protein gp120 are reported to interact with CD26. These observations indicate that CD26 is closely involved in HIV cell entry and that CD26-mediatod T cell immune response is suppressed. In addition. it has been demonstrated that the anti-HIV and chemotactic activities of RANTES (regulated on activation. normal T cell expressed and secreted) and stromal cell-derived factor-1 (SDF-1) are controlled with the DPPIV activity of CD26. Thus. the regulation of the function of chemokines by CD26/DPPIV appears to be essential fbr lymphocyte trafficking and infectivity of HIV strains. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

DOI： 10.1016/S0923-1811(99)00081-X

Web of Science
1027 Isolation and cultivation of fungi on palm oil mill fiber under higher temperature :

Suyanto, Yazaki Shin-ichi, Ohtsuki Takashi, Ui Sadaharu, Mimura Akio

12 246 - 246 2000

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Fungous decomposition of rice straw under higher temperature

Ohtsuki Takashi, Suyanto, Yazaki Shin-ichi, Ui Sasaharu, Mimura Akio

12 246 - 246 2000

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Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4T cells Reviewed Major achievement

Mukasa R, Homma T, Ohtsuki T, Hosono O, Souta A, Kitamura T, Fukuda M, Watanabe S, Morimoto C

International Immunology 11 ( 2 ) 259 - 268 1999.2

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Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells

R Mukasa, T Homma, T Ohtsuki, O Hosono, A Souta, T Kitamura, M Fukuda, S Watanabe, C Morimoto

INTERNATIONAL IMMUNOLOGY 11 ( 2 ) 259 - 268 1999.2( ISSN：0953-8178 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO(+) memory subset and a CD45RA(+) naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4, The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral Ca4 T cells and 1D4(+) CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1D4 antigen is human CD43, Transfection of CHO-leu cells, which stably express human CD43, with core 2 beta-1,6-N-acetylslucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only In 1D4(+) T cells but not in 1D4(-) T cells, implying that the 1D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1D4 was completely abolished when cells were treated with neuraminidase, while there remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans, Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT; whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305, Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO(+) memory subset of CD4 T cells.

DOI： 10.1093/intimm/11.2.259

Web of Science

PubMed
Core 2-containing O-glycans on CD43 are preferentially expressed in the memory subset of human CD4 T cells Reviewed

R Mukasa, T Homma, T Ohtsuki, O Hosono, A Souta, T Kitamura, M Fukuda, S Watanabe, C Morimoto

INTERNATIONAL IMMUNOLOGY 11 ( 2 ) 259 - 268 1999.2( ISSN：0953-8178 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：OXFORD UNIV PRESS

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO(+) memory subset and a CD45RA(+) naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4, The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral Ca4 T cells and 1D4(+) CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1D4 antigen is human CD43, Transfection of CHO-leu cells, which stably express human CD43, with core 2 beta-1,6-N-acetylslucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only In 1D4(+) T cells but not in 1D4(-) T cells, implying that the 1D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1D4 was completely abolished when cells were treated with neuraminidase, while there remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans, Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT; whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305, Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO(+) memory subset of CD4 T cells.

DOI： 10.1093/intimm/11.2.259

Web of Science

PubMed
Negative regulation of the anti-human immunodeficiency virus and chemotactic activity of human stromal cell-derived factor1alpha by CD26/dipeptidyl peptidase IV Reviewed Major achievement

Ohtsuki T, Hosono O, Kobayashi H, Munakata Y, Souta A, Shioda T, Morimoto C

FEBS Letters 431 ( 2 ) 236 - 240 1998.7

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Negative regulation of the anti-human immunodeficiency virus and chemotactic activity of human stromal cell-derived factor 1 alpha by CD26/dipeptidyl peptidase IV Reviewed

T Ohtsuki, O Hosono, H Kobayashi, Y Munakata, A Souta, T Shioda, C Morimoto

FEBS LETTERS 431 ( 2 ) 236 - 240 1998.7( ISSN：0014-5793 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Stromal cell-derived factor 1 alpha (SDF-1 alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this studg, we show that SDF-1 alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory activity of SDF-1 alpha against HIV infection disappeared, Moreover, the chemotactic activity of SDF-1 alpha also disappeared specifically by DPPIV activity of recombinant soluble CD26, These results suggested that dissemination of T-tropic HIV strains in vivo may be facilitated by CD26/DPPIV via inactivation of functional SDF-1 alpha. (C) 1998 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(98)00763-7

Web of Science

PubMed
Negative regulation of the anti-human immunodeficiency virus and chemotactic activity of human stromal cell-derived factor 1 alpha by CD26/dipeptidyl peptidase IV Reviewed

T Ohtsuki, O Hosono, H Kobayashi, Y Munakata, A Souta, T Shioda, C Morimoto

FEBS LETTERS 431 ( 2 ) 236 - 240 1998.7( ISSN：0014-5793 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

Stromal cell-derived factor 1 alpha (SDF-1 alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this studg, we show that SDF-1 alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory activity of SDF-1 alpha against HIV infection disappeared, Moreover, the chemotactic activity of SDF-1 alpha also disappeared specifically by DPPIV activity of recombinant soluble CD26, These results suggested that dissemination of T-tropic HIV strains in vivo may be facilitated by CD26/DPPIV via inactivation of functional SDF-1 alpha. (C) 1998 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(98)00763-7

Web of Science

PubMed
Expression of genes encoding membrane-bound hydrogenase in Pseudomonas hydrogenovora under autotrophic condition is dependent on two different promoters Reviewed Major achievement

Ohtsuki T, Shimosaka M, Okazaki M

Bioscience, Biotechnology, and Biochemistry 61 ( 12 ) 1986 - 1990 1997.12

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Expression of genes encoding membrane-bound hydrogenase in Pseudomonas hydrogenovora under autotrophic condition is dependent on two different promoters

T Ohtsuki, M Shimosaka, M Okazaki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 61 ( 12 ) 1986 - 1990 1997.12( ISSN：0916-8451 eISSN：1347-6947 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD

Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative sigma(54)-type promoter sequences (P-hup1, P-hup2, and P-hup3). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that P-hup1 and P-hup3 would be essential for a transcriptional initiation. It was also found that the region upstream of P-hup sequences was concerned with regulation of transcription.

DOI： 10.1271/bbb.61.1986

Web of Science

PubMed
Expression of genes encoding membrane-bound hydrogenase in Pseudomonas hydrogenovora under autotrophic condition is dependent on two different promoters Reviewed

T Ohtsuki, M Shimosaka, M Okazaki

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY 61 ( 12 ) 1986 - 1990 1997.12( ISSN：0916-8451 eISSN：1347-6947 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：TAYLOR & FRANCIS LTD

Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative sigma(54)-type promoter sequences (P-hup1, P-hup2, and P-hup3). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that P-hup1 and P-hup3 would be essential for a transcriptional initiation. It was also found that the region upstream of P-hup sequences was concerned with regulation of transcription.

DOI： 10.1271/bbb.61.1986

Web of Science

PubMed
水素細菌Pseudomonas hydrogenovoraのヒドロゲナーゼ遺伝子群の構造と発現 Reviewed Major achievement

大槻隆司

信州大学 1997.9

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Language：Japanese Publishing type：Doctoral Thesis
The hupC gene product is a component of the electron transport system for hydrogen oxidation in Pseudomonas hydrogenovora Major achievement

T Ohtsuki, Y Kita, T Fujioka, D Hashimoto, M Shimosaka, M Okazaki

FEMS MICROBIOLOGY LETTERS 150 ( 1 ) 127 - 133 1997.5( ISSN：0378-1097 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

The hydrogenase gene cluster containing nine genes (hupSLCDFGHIJ) was identified by sequencing of an 8.8-kb DNA region from Pseudomonas hydrogenovora. To investigate the function of the hupC gene product, we isolated a hupC-null mutant (HID3) of P. hydrogenovora by introducing an in-frame deletion into the hupC. The mutant, HID3, could not grow autotrophically but retained half the level of hydrogenase activity of the wild-type strain. Results of the oxygen consumption test and Western blot analysis revealed that the hupC gene product is a b-type cytochrome but not involved in the hydrogenase maturation process.

DOI： 10.1016/S0378-1097(97)00108-0

Web of Science
The hupC gene product is a component of the electron transport system for hydrogen oxidation in Pseudomonas hydrogenovora Reviewed Major achievement

T Ohtsuki, Y Kita, T Fujioka, D Hashimoto, M Shimosaka, M Okazaki

FEMS MICROBIOLOGY LETTERS 150 ( 1 ) 127 - 133 1997.5( ISSN：0378-1097 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

The hydrogenase gene cluster containing nine genes (hupSLCDFGHIJ) was identified by sequencing of an 8.8-kb DNA region from Pseudomonas hydrogenovora. To investigate the function of the hupC gene product, we isolated a hupC-null mutant (HID3) of P. hydrogenovora by introducing an in-frame deletion into the hupC. The mutant, HID3, could not grow autotrophically but retained half the level of hydrogenase activity of the wild-type strain. Results of the oxygen consumption test and Western blot analysis revealed that the hupC gene product is a b-type cytochrome but not involved in the hydrogenase maturation process.

DOI： 10.1016/S0378-1097(97)00108-0

Web of Science

PubMed
The hupC gene product is a component of electron transport system for hydrogen oxidation in Pseudomonas hydrogenovora Reviewed Major achievement

Ohtsuki T, Kita Y, Fujioka T, Hashimoto D, Shimosaka M, Okazaki M

FEMS Microbiology Letters 150 ( 1 ) 127 - 133 1997.3

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
Cloning and sequencing of the membrane-bound hydrogenase-encoding genes (hupS and hupL) from Pseudomonas hydrogenovora Reviewed Major achievement

Ohtsuki T, Okazaki T, Endo Y, Kita Y, Shimosaka M, Okazaki M

Gene 166 ( 1 ) 89 - 93 1995.12

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)
CLONING AND SEQUENCING OF THE MEMBRANE-BOUND HYDROGENASE-ENCODING GENES (HUPS AND HUPL) FROM PSEUDOMONAS-HYDROGENOVORA

T OHTSUKI, T OKAZAKI, Y ENDO, Y KITA, M SHIMOSAKA, M OKAZAKI

GENE 166 ( 1 ) 89 - 93 1995.12( ISSN：0378-1119 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

The membrane-bound hydrogenase (Hdg)-encoding structural genes were isolated from the hydrogen-utilizing bacterium Pseudomonas hydrogenovora (Ph). Nucleotide sequence analysis revealed two genes, hupS and hupL. The hupS gene encoded a 363-amino-acid (aa) polypeptide (40.4 kDa). The deduced aa sequence contained a putative 43-aa leader peptide sequence. The hupL gene started at 55 bp downstream from the stop codon of hupS and encoded a 622-aa polypeptide (69.3 kDa). The disruption of Ph hupS resulted in the loss of Hdg activity.

DOI： 10.1016/0378-1119(95)00580-4

Web of Science

PubMed
CLONING AND SEQUENCING OF THE MEMBRANE-BOUND HYDROGENASE-ENCODING GENES (HUPS AND HUPL) FROM PSEUDOMONAS-HYDROGENOVORA Reviewed

T OHTSUKI, T OKAZAKI, Y ENDO, Y KITA, M SHIMOSAKA, M OKAZAKI

GENE 166 ( 1 ) 89 - 93 1995.12( ISSN：0378-1119 )

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Language：English Publishing type：Research paper (scientific journal) Publisher：ELSEVIER SCIENCE BV

The membrane-bound hydrogenase (Hdg)-encoding structural genes were isolated from the hydrogen-utilizing bacterium Pseudomonas hydrogenovora (Ph). Nucleotide sequence analysis revealed two genes, hupS and hupL. The hupS gene encoded a 363-amino-acid (aa) polypeptide (40.4 kDa). The deduced aa sequence contained a putative 43-aa leader peptide sequence. The hupL gene started at 55 bp downstream from the stop codon of hupS and encoded a 622-aa polypeptide (69.3 kDa). The disruption of Ph hupS resulted in the loss of Hdg activity.

DOI： 10.1016/0378-1119(95)00580-4

Web of Science

PubMed
水素細菌Pseudomonas hydrogenovoraの有するヒドロゲナーゼ遺伝子の構造解析 Reviewed Major achievement

大槻隆司

信州大学 1994.3

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Language：Japanese Publishing type：Master Thesis

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Books and Other Publications

未培養微生物の循環型社会への有効活用 Major achievement

大槻隆司（ Role： Contributor第７章）

シーエムシー出版 2023.4

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Total pages：264 Responsible for pages：212-219 Language：Japanese Book type：Scholarly book
バイオ系のキャリアデザイン　〜　反省はするが後悔はしない

大槻隆司（ Role： Single Work）

2022.2

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Responsible for pages：100(2): 90-95 Language：Japanese
バイオマスの有効利用を妨げる渋滞問題 Major achievement

大槻隆司（90-2）

2012.2

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Responsible for pages：93- Language：Japanese
水素利用インフラ整備は微生物界でも大変!? Major achievement

大槻隆司（-）

2009.3

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Responsible for pages：135- Language：Japanese
オンサイトでの燃料電池発電のための水素生産ユニット～バイオによる家庭用燃料生産システムの現状と課題～ Major achievement

大槻隆司（-）

（株）エヌ・ティー・エス 2006.10

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Responsible for pages：651-655 Language：Japanese
ファイバー・スーパーバイオミメティックス

（ Role： Contributorオンサイトでの燃料電池発電のための水素生産ユニット－バイオによる家庭用燃料生産システムの現状と課題－）

（株）エヌ・ティー・エス 2006

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Language：Japanese
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. I. Biomass utilization of oil palm plantations in Indonesia: present status, problems and prospects

M. Ahkam Subroto,Takashi Ohtsuki,他４名（-）

2005

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Responsible for pages：364-370 Language：English
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. II. Development of in-field composting system by solid state fermentation process with thermophilic fungus for sustainable prosperity of oil palm plantations in Indonesia Major achievement

Takashi Ohtsuki,他５名（-）

2005

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Responsible for pages：371-381 Language：English
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. I. Biomass utilization of oil palm plantations in Indonesia: present status, problems and prospects

2005

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Language：English
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. II. Development of in-field composting system by solid state fermentation process with thermophilic fungus for sustainable prosperit・・・

2005

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Language：English

Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. II. Development of in-field composting system by solid state fermentation process with thermophilic fungus for sustainable prosperity of oil palm plantations in Indonesia
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. I. Biomass utilization of oil palm plantations in Indonesia: present status, problems and prospects Major achievement

Akio Mimura,Takashi Ohtsuki,他４名（-）

2004

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Responsible for pages：353-358 Language：English
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. II. Development of rapid decomposition process of palm oil mill wastes Major achievement

Akio Mimura,Takashi Ohtsuki,他４名（-）

2004

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Responsible for pages：347-352 Language：English
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. I. Biomass utilization of oil palm plantations in Indonesia: present status, problems and prospects

2004

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Language：English
Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. II. Development of rapid decomposition process of palm oil mill wastes

2004

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Language：English
Development of in situ bioactivator process on palm oil mill fiber by Chaetomium sp. nov. MS-017 Major achievement

Suyanto,Takashi Ohtsuki,他４名（-）

2003

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Responsible for pages：378-384 Language：English
Development of in situ bioactivator process on palm oil mill fiber by Chaetomium sp. nov. MS-017

2003

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Language：English
Integrated development of biomass plantations for energy, and commodity and fine chemicals Major achievement

Akio Mimura,Suyanto,Takashi Ohtsuki（-）

2002.8

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Responsible for pages：PDF-1045 Language：English
Integrated development of biomass plantations for energy, and commodity and fine chemicals

2002

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Language：English
Composting of palm oil mill fiber by fungi under higher temperature Major achievement

Akio Mimura,Suyanto,Shin-ichi Yazaki,Takashi Ohtsuki,Sadaharu Ui（-）

2001.1

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Responsible for pages：920-923 Language：English
Composting of palm oil mill fiber by fungi under higher temperature

2001

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Language：English

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Presentations

下水処理場の臭気に関与する微生物の臭気抑制手段の探索 Major achievement

本門慧史、武井良二、大槻隆司

第７５回日本生物工学会大会 2023.9

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Event date： 2023.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：名古屋
群体性藻類Botryococcus braunii単細胞培養条件の応答局面法による最適化 Major achievement

村山拳午、大槻隆司

第７５回日本生物工学会大会 2023.9

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Event date： 2023.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：名古屋
微細藻類Botryococcus brauniiの単細胞を迅速にコロニー再生させる培養条件の検討

村山拳午、大槻隆司

生物工学若手研究者の集い夏のセミナー2023 2023.6 日本生物工学会

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Event date： 2023.6

Language：Japanese Presentation type：Oral presentation(general)

Venue：富山県砺波青少年自然の家
メッキ工場排水処理汚泥由来細菌が亜鉛除去能を発揮する培養条件の検討

栁原隆志、大槻隆司

生物工学若手研究者の集い夏のセミナー2023 2023.6 日本生物工学会

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Event date： 2023.6

Language：Japanese Presentation type：Oral presentation(general)

Venue：富山県砺波青少年自然の家
道志川の 2-メチルイソボルネオ―ル生成に関与する微生物および環境要因の解析

奥野凌、大槻隆司

生物工学若手研究者の集い夏のセミナー2023 2023.6 日本生物工学会

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Event date： 2023.6

Language：Japanese Presentation type：Oral presentation(general)

Venue：富山県砺波青少年自然の家
Serratia marcescens の2,3-ブタンジオール生産性に関与する遺伝子の機能解析

橋間ゆきの、大槻隆司

生物工学若手研究者の集い夏のセミナー2023 2023.6 日本生物工学会

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Event date： 2023.6

Language：Japanese Presentation type：Oral presentation(general)

Venue：富山県砺波青少年自然の家
群体性藻類Botryococcus braunii の機能解析や分子育種に資する単細胞培養条件の確立 Major achievement

村山拳午、大槻隆司

第７４回日本生物工学会大会 2022.10

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Event date： 2022.10

Language：Japanese Presentation type：Oral presentation(general)

Venue：オンライン
道志川における2-メチルイソボルネオ―ル生成微生物と環境要因に関する解析 Major achievement

奥野凌、大槻隆司

第７４回日本生物工学会大会 2022.10

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Event date： 2022.10 - 2023.10

Language：Japanese Presentation type：Oral presentation(general)

Venue：オンライン
バイオマスは化石資源を代替できるか　〜バイオマス利用の現状と課題〜 Invited

大槻隆司

日本技術士会山梨支部例会 2022.5 日本技術士会山梨支部

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Event date： 2022.5

Language：Japanese Presentation type：Public discourse, seminar, tutorial, course, lecture and others

Venue：甲府
シロアリ腸内から分離した新規細菌の取得とゲノム解析

森浩佐, 雪真弘, 飯野隆夫, 野田悟子, 大槻隆司, 大熊盛也

日本微生物生態学会第34回大会 2021.11

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Event date： 2021.11

Language：Japanese Presentation type：Poster presentation
シロアリ腸内から分離したBacteroidetes門の新規性細菌のゲノム解析

森浩佐, 雪真弘, 飯野隆夫, 野田悟子, 大槻隆司, 大熊盛也

日本微生物資源学会第27回大会 2021.6

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Event date： 2021.6

Language：Japanese Presentation type：Oral presentation(general)
雑草中の水溶性成分および非水溶性成分をそれぞれ基質としたメタン発酵における微生物相解析 Major achievement

○松田修平、大槻隆司

第７１回(2019)日本生物工学会大会 2019.9

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Event date： 2019.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：岡山大学津島キャンパス
炭化水素生産微細藻類Botryococcus brauniiの共生細菌が生産する藻体生育促進物質の探索 Major achievement

○村山拳午、大槻隆司

第７１回(2019)日本生物工学会大会 2019.9

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Event date： 2019.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：岡山大学津島キャンパス
廃飲料メタン発酵における基質高負荷時の菌相変動解析 Major achievement

○松田修平、山登貴広、望月誉志幸、関口喜則、大槻隆司

日本微生物生態学会第３３回大会 2019.9

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Event date： 2019.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学甲府キャンパス
廃棄雑草のみを原料としたメタン発酵における雑草溶出成分のメタン生産への影響 Major achievement

松田修平、大槻隆司

日本農芸化学会2019年度大会 2019.3

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Event date： 2019.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：東京農業大学（東京）
雑草を原料とした高C/N比メタン発酵における微生物群構成種の単離

松田修平、大槻隆司

平成28年度日本生物工学会大会 2016.9

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Event date： 2016.9

Language：Japanese Presentation type：Poster presentation

Venue：富山国際会議場（富山）
Serratia marcescensにおいて2,3-ブタンジオール生産性を増大させる変異の解析 Major achievement

張琳、大槻隆司

平成28年度日本生物工学会大会 2016.9

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Event date： 2016.9

Language：Japanese Presentation type：Poster presentation

Venue：富山国際会議場（富山）
特異的にナノセルロース分解を行う微生物の単離 Major achievement

高橋仁志、大槻隆司

平成28年度日本生物工学会大会 2016.9

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Event date： 2016.9

Language：Japanese Presentation type：Poster presentation

Venue：富山国際会議場（富山）
雑草処理に特化した安定メタン発酵菌群の取得 Major achievement

松田修平,大槻隆司

平成27年度日本生物工学会大会 2015.10 平成２７年度日本生物工学会大会

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Event date： 2015.10

Language：Japanese Presentation type：Poster presentation

Venue：城山観光ホテル（鹿児島）
2,3-ブタンジオール高生産菌の選択と生産条件の最適化 Major achievement

大槻隆司,望月隆一,青木信雄,宇井定春

日本農芸化学会2015年度大会 2015.3 日本農芸化学会２０１５年度大会

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Event date： 2015.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：岡山大学津島キャンパス
食用キノコを用いた木質バイオマス易利用化処理の検討 Major achievement

長田萌,大槻隆司,宇井定春

平成26年度日本生物工学会大会 2014.9 平成２６年度日本生物工学会大会

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Event date： 2014.9

Language：Japanese Presentation type：Poster presentation

Venue：札幌コンベンションセンター
アグロバクテリウムを用いたCeriporiopsis subvermispora形質転換法の開発

鈴木亜美,大槻隆司,宇井定春

平成26年度日本生物工学会大会 2014.9 平成２６年度日本生物工学会大会

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Event date： 2014.9

Language：Japanese Presentation type：Poster presentation

Venue：札幌コンベンションセンター
Botryococcus brauniiの生育およびオイル生産性を促進する共存細菌の単離 Major achievement

大槻隆司,菅内雄一郎,宇井定春

平成26年度日本生物工学会大会 2014.9 平成２６年度日本生物工学会大会

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Event date： 2014.9

Language：Japanese Presentation type：Poster presentation

Venue：札幌コンベンションセンター
腐植酸資材による活性汚泥処理能力向上 Major achievement

大槻隆司,花形麻美,宇井定春

平成２５年度日本生物工学会大会 2013.9 日本生物工学会

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Event date： 2013.9

Language：Japanese Presentation type：Poster presentation

Venue：広島国際会議場
食材性昆虫に共生する原生生物の木質分解酵素遺伝子の多型解析

行仕圭佑,雪真弘,大槻隆司,大熊盛也,宇井定春,野田悟子

第28回日本微生物生態学会大会 2012.9 日本微生物生態学会

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Event date： 2012.9

Language：Japanese Presentation type：Poster presentation

Venue：豊橋技術科学大学
Relationship between preservation stability and salt concentration of L(2S,3S)-2,3-butanediol dehydrogenase

Shimegi T, Ohtsuki T, Kurisu G, Kusunoki M, Ui S

第12回日本蛋白質科学会年会 2012.6

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Event date： 2012.6

Language：English Presentation type：Poster presentation
活性汚泥処理能力に及ぼす腐植酸の影響 Major achievement

花形麻美,的場梢,大槻隆司,野田悟子,宇井定春

2011年度日本生物工学会若手研究者の集い夏のセミナー 2011.7 日本生物工学会若手研究者の集い

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Event date： 2011.7

Language：Japanese Presentation type：Poster presentation

Venue：春日居びゅーほてる
改変型2,3-butanediol dehydrogenaseの立体構造解析

松井義由貴,大槻隆司,野田悟子、楠木正巳、宇井定春

2011年度日本生物工学会若手研究者の集い夏のセミナー 2011.7 日本生物工学会若手研究者の集い

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Event date： 2011.7

Language：Japanese Presentation type：Poster presentation

Venue：春日居びゅーほてる
半硬質チーズの保存中における品質劣化と微生物相変化の解析

榊原唯起,大槻隆司,野田悟子,宇井定春

2011年度日本生物工学会若手研究者の集い夏のセミナー 2011.7 日本生物工学会若手研究者の集い

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Event date： 2011.7

Language：Japanese Presentation type：Poster presentation

Venue：春日居びゅーほてる
改変型2,3-butanediol dehydrogenaseの立体構造解析

2011年度日本生物工学会若手研究者の集い夏のセミナー 2011

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Language：Japanese Presentation type：Poster presentation
活性汚泥処理能力に及ぼす腐植酸の影響

2011年度日本生物工学会若手研究者の集い夏のセミナー 2011

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Language：Japanese Presentation type：Poster presentation
シロアリ類に共生するSpirotrichonymphida目原生生物の分子系統解析

高柳美希,栗崎倫江,大槻隆司,宇井定春,大熊盛也,野田悟子

第43回日本原生動物学会大会 2010.11

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Event date： 2010.11

Language：Japanese Presentation type：Poster presentation

Venue：茨城大学
微小化セルロースを資化する微生物群の菌相解析 Major achievement

大槻隆司,尾塩岳治,林田稔,下坂誠

平成22年度日本生物工学会大会 2010.10 日本生物工学会

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Event date： 2010.10

Language：Japanese Presentation type：Poster presentation

Venue：宮崎ワールドコンベンションセンターサミット
シロアリの共生Spirotrichonymphea綱原生生物の分子系統解析

高柳美希、北出理，大槻隆司、宇井定春、大熊盛也、野田悟子

第27回日本微生物生態学会 2010.10

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Event date： 2010.10

Language：Japanese Presentation type：Oral presentation(general)

Venue：京都大学
UV/オゾン処理と白色・褐色腐朽菌を用いた腐朽処理の組合せによるヒノキ材易利用化の検討 Major achievement

大槻隆司,野田悟子,宇井定春

2010年度日本農芸化学会関東支部大会 2010.10 日本農芸化学会関東支部

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Event date： 2010.10

Language：Japanese Presentation type：Poster presentation

Venue：千葉大学
木質資源の直接微生物変換効率化のための腐朽処理条件の検討 Major achievement

大槻隆司,野田悟子,宇井定春

2010年度日本農芸化学会大会 2010.3 日本農芸化学会

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Event date： 2010.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：東京大学
微小化セルロースを資化する微生物群の菌相解析

平成22年度日本生物工学会大会 2010

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Language：Japanese Presentation type：Poster presentation
UV/オゾン処理と白色・褐色腐朽菌を用いた腐朽処理の組合せによるヒノキ材易利用化の検討

2010年度日本農芸化学会関東支部大会 2010

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Language：Japanese Presentation type：Poster presentation
木質資源の直接微生物変換効率化のための腐朽処理条件の検討

2010年度日本農芸化学会大会 2010

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Language：Japanese Presentation type：Oral presentation(general)
高フェノール濃度馴化活性汚泥への腐植酸添加によるフェノール除去能の促進 Major achievement

大槻隆司,櫻井一樹,野田悟子,市原譲治,増田隆仁,宇井定春

2009年度日本農芸化学会関東支部大会 2009.10 日本農芸化学会関東支部

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Event date： 2009.10

Language：Japanese Presentation type：Poster presentation

Venue：玉川大学
木質バイオマスへの糖化酵素作用性を高める処理条件の検討 Major achievement

大槻隆司,早川正幸,宇井定春

2009年度日本農芸化学会大会 2009.3 日本農芸化学会

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Event date： 2009.3

Language：Japanese Presentation type：Poster presentation

Venue：マリンメッセ福岡
木質バイオマスへの糖化酵素作用性を高める処理条件の検討

2009年度日本農芸化学会大会 2009

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Language：Japanese Presentation type：Poster presentation
高フェノール濃度馴化活性汚泥への腐植酸添加によるフェノール除去能の促進

2009年度日本農芸化学会関東支部大会 2009

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Language：Japanese Presentation type：Poster presentation
Subdomain chimeraによる有用SDRの創製とその立体構造

大畑進,山下哲生,大槻隆司,楠木正巳,宇井定春

2008年度日本農芸化学会関東支部大会 2008.10 日本農芸化学会関東支部

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Event date： 2008.10

Language：Japanese Presentation type：Poster presentation

Venue：山梨大学工学部
セルロース分解微生物群制御をめざした機能微生物解析 Major achievement

三上篤,大槻隆司,矢崎伸一,宇井定春

2008年度日本農芸化学会大会 2008.3 日本農芸化学会

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Event date： 2008.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：名城大学
セルロース分解微生物群制御をめざした機能微生物解析

2008年度日本農芸化学会大会 2008

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Language：Japanese Presentation type：Oral presentation(general)
Subdomain chimeraによる有用SDRの創製とその立体構造

2008年度日本農芸化学会関東支部大会 2008

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Language：Japanese Presentation type：Poster presentation
水素生産複合系における機能微生物の解析 Major achievement

山村陽介,矢崎伸一,大槻隆司,宇井定春

2007年度日本農芸化学会大会 2007.3 日本農芸化学会

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Event date： 2007.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：東京農業大学
水素生産複合系における機能微生物の解析

2007年度日本農芸化学会大会 2007

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Language：Japanese Presentation type：Oral presentation(general)
オウゴン（黄金花根部抽出生薬）フラボノイド成分Wogoninの抗腫瘍活性の特性

三村精男、姫地実加、深沢晴美、田中美穂、大槻隆司、山本久子

第１３回日本がん予防学会 2006.7

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Event date： 2006.7

Language：Japanese Presentation type：Oral presentation(general)

Venue：京都
沖縄薬膳素材のヒト二倍体細胞を用いる抗紫外線障害活性

三村精男、下地純華、大槻隆司

第３会国際食品機能学会研究発表会 2006.5

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Event date： 2006.5

Language：Japanese Presentation type：Oral presentation(general)

Venue：東京ビックサイト
パーム油搾油粕の高温糸状菌固体醗酵による抗菌性物質の生産 Major achievement

板橋輝彦,矢崎伸一,大槻隆司,他３名

２００６年度日本農芸化学会大会 2006.3 日本農芸化学会

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Event date： 2006.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：京都女子大学
インドネシアジャムーによるヒトマクロファージ様細胞活性化 Major achievement

雨宮高嗣,清水麻里,大槻隆司，矢崎伸一、宇井定春、三村精男

２００６年度日本農芸化学会大会 2006.3 日本農芸化学会

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Event date： 2006.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：京都女子大学
拮抗微生物を活用したマンゴー炭疽病菌の予防的抑制 Major achievement

佐々木利奈,大槻隆司,矢崎伸一,宇井定春,三村精男

２００６年度日本農芸化学会大会 2006.3 日本農芸化学会

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Event date： 2006.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：京都女子大学
インドネシアジャムーによるヒトマクロファージ様細胞活性化

２００６年度日本農芸化学会大会 2006

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Language：Japanese Presentation type：Oral presentation(general)
拮抗微生物を活用したマンゴー炭疽病菌の予防的抑制

２００６年度日本農芸化学会大会 2006

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Language：Japanese Presentation type：Oral presentation(general)
パーム油搾油粕の高温糸状菌固体醗酵による抗菌性物質の生産

２００６年度日本農芸化学会大会 2006

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Language：Japanese Presentation type：Oral presentation(general)
Microbial biotechnology for sustainable agriculture of peat land International conference

Mimura A, Ohtsuki T, Koesnandar

9th National Congress of Indonesian Society for Microbiology & 3rd Asian Conference for Lactic Acid Bacteria 2005.8

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Event date： 2005.8

Language：English Presentation type：Oral presentation(general)
Modification of peat soil of Kalimantan by solid state fermentation of soil microorganisms and plant biomass

Mimura A, Sakata H, Nakano M, Inoue J, Yazaki S, Ohtsuki T, Wahjono E, Koesnandar

9th National Congress of Indonesian Society for Microbiology & 3rd Asian Conference for Lactic Acid Bacteria 2005.8

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Event date： 2005.8

Language：English Presentation type：Oral presentation(general)
ヒト二倍体細胞を用いる紫外線傷害評価法の構築と沖縄薬膳素材、抗酸化ビタミンへの応用

三村精男,下地純華,石原愛弓,大槻隆司,宇井定春、矢崎伸一

第１２回日本がん予防研究会・第２８回日本がん疫学研究会 2005.7

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Event date： 2005.7

Language：Japanese Presentation type：Poster presentation
Microbial biotechnology for sustainable agriculture of peat land International coauthorship International conference

9th National Congress of Indonesian Society for Microbiology & 3rd Asian Conference for Lactic Acid Bacteria 2005

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Language：English Presentation type：Oral presentation(general)
ヒト二倍体細胞を用いる紫外線傷害評価法の構築と沖縄薬膳素材、抗酸化ビタミンへの応用

第１２回日本がん予防研究会・第２８回日本がん疫学研究会 2005

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Language：Japanese Presentation type：Oral presentation(general)
Modification of peat soil of Kalimantan by solid state fermentation of soil microorganisms and plant biomass International coauthorship International conference

9th National Congress of Indonesian Society for Microbiology & 3rd Asian Conference for Lactic Acid Bacteria 2005

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Language：English Presentation type：Oral presentation(general)
Construction of hybrid microbial consortium for production of bio-hydrogen from tropical biomass International conference Major achievement

Ohtsuki T, Itoh K, Yazaki S, Ui S, Mimura A

The 3rd Indonesian Biotechnology Conference 2004.12

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Event date： 2004.12

Language：English Presentation type：Poster presentation
Development of bioactivator process on empty fruit bunches by indigenous microbial consortium International conference Major achievement

Suyanto, Ohtsuki T, Subroto MA, Haska N, Koesnandar, Mimura A

The 3rd Indonesian Biotechnology Conference 2004.12

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Event date： 2004.12

Language：English Presentation type：Poster presentation
Elucidation of tropical health food factors by analyzing radical scavenging activity for the prevention of life style-related diseases International conference

Mimura A, Mukouyama M, Kitai H, Yazaki S, Ohtsuki T, Ui S, Widjayanti RDE, Koesnandar

The 3rd Indonesian Biotechnology Conference 2004.12

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Event date： 2004.12

Language：English Presentation type：Poster presentation
Production of xylanase complex and antifungal compounds by solid state fermentation of palm oil mill fiber using Chaetomium sp. nov. MS-017 International conference Major achievement

Ohtsuki T, Suyanto, Itabashi T, Yazaki S, Ui S, Mimura A

The 3rd Indonesian Biotechnology Conference 2004.12

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Event date： 2004.12

Language：English Presentation type：Poster presentation
Evaluation of anti-UVB components of tropical medicinal plants by using human fetal lung diploid cell line TIG-1 International conference

Shimoji S, Himeji M, Yazaki S, Ohtsuki T, Ui S, Widjayanti RDE, Koesnandar, Mimura A

The 3rd Indonesian Biotechnology Conference 2004.12

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Event date： 2004.12

Language：English Presentation type：Poster presentation
Development of biological control system for repressing stem rotting mushroom Ganoderma spp. on oil palm trees and anthracrose fungi Collectorichum spp. on mango fruits International conference

Nakano M, Sasaki R, Ohtsuki T, Yazaki S, Ui S, Subroto MA< Mimura A

The 3rd Indonesian Biotechnology Conference 2004.12

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Event date： 2004.12

Language：English Presentation type：Poster presentation

Venue：Bali, Indonesia
役割の異なる微生物群の複合化による水素生産システムの構築 Major achievement

大槻隆司,伊藤王人,矢崎伸一,宇井定春,三村精男

平成１６年度日本生物工学会大会 2004.9 日本生物工学会

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Event date： 2004.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：名城大学
ヒト細胞系TIG-1を用いる沖縄薬膳素材の抗紫外線物質の探索

下地純華,姫地実加,大槻隆司,矢崎伸一、宇井定春、三村精男

平成１６年度日本生物工学会大会 2004.9 日本生物工学会

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Event date： 2004.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：名城大学
干潟複合微生物系モデルを指標とした森林、干潟の微生物生態関連性の解析

井上潤一,矢崎伸一,大槻隆司,宇井定春,三村精男

平成１６年度日本生物工学会大会 2004.9 日本生物工学会

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Event date： 2004.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：名城大学
アブラヤシ樹木腐朽性Ganoderma拮抗微生物による生物制御

中野理子,秋積宏伸,M. Ahkam Subroto,矢崎伸一,大槻隆司,宇井定春、三村精男

平成１６年度日本生物工学会大会 2004.9 日本生物工学会

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Event date： 2004.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：名城大学
サトウキビ食文化は奄美大島の長寿に関連するのか？

三村精男,鈴木賢洋,矢崎伸一,大槻隆司,宇井定春、兵藤文則

第１１回日本がん予防研究会 2004.7

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Event date： 2004.7

Language：Japanese Presentation type：Oral presentation(general)
干潟複合微生物系モデルによる森林・河川と干潟の微生物生態学的解析

井上潤一,矢崎伸一,大槻隆司,宇井定春,三村精男

第７回マリンバイオテクノロジー学会大会 2004.6

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Event date： 2004.6

Language：Japanese Presentation type：Oral presentation(general)

Venue：北海道大学
Elucidation of tropical health food factors by analyzing radical scavenging activity for the prevention of life style-related diseases International conference

The 3rd Indonesian Biotechnology Conference 2004

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Language：English Presentation type：Oral presentation(general)
Production of xylanase complex and antifungal compounds by solid state fermentation of palm oil mill fiber using Chaetomium sp. nov. MS-017

The 3rd Indonesian Biotechnology Conference 2004

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Language：English Presentation type：Poster presentation
Evaluation of anti-UVB components of tropical medicinal plants by using human fetal lung diploid cell line TIG-1

The 3rd Indonesian Biotechnology Conference 2004

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Language：English Presentation type：Poster presentation
Development of biological control system for repressing stem rotting mushroom Ganoderma spp. on oil palm trees and anthracrose fungi Collectorichum spp. on mango fruits

The 3rd Indonesian Biotechnology Conference 2004

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Language：English Presentation type：Poster presentation
Development of bioactivator process on empty fruit bunches by indigenous microbial consortium

The 3rd Indonesian Biotechnology Conference 2004

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Language：English Presentation type：Poster presentation
Construction of hybrid microbial consortium for production of bio-hydrogen from tropical biomass

The 3rd Indonesian Biotechnology Conference 2004

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Language：English Presentation type：Poster presentation
役割の異なる微生物群の複合化による水素生産システムの構築

平成１６年度日本生物工学会大会 2004

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Language：Japanese Presentation type：Oral presentation(general)
干潟複合微生物系モデルを指標とした森林、干潟の微生物生態関連性の解析

平成１６年度日本生物工学会大会 2004

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Language：Japanese Presentation type：Oral presentation(general)
干潟複合微生物系モデルによる森林・河川と干潟の微生物生態学的解析

第７回マリンバイオテクノロジー学会大会 2004

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Language：Japanese Presentation type：Oral presentation(general)
ヒト細胞系TIG-1を用いる沖縄薬膳素材の抗紫外線物質の探索

平成１６年度日本生物工学会大会 2004

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Language：Japanese Presentation type：Oral presentation(general)
サトウキビ食文化は奄美大島の長寿に関連するのか？

第１１回日本がん予防研究会 2004

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Language：Japanese Presentation type：Oral presentation(general)
アブラヤシ樹木腐朽性Ganoderma拮抗微生物による生物制御

平成１６年度日本生物工学会大会 2004

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Language：Japanese Presentation type：Oral presentation(general)
Induction of apoptosis in human leukemia cells by a natural fermented sugar cane vinegar (Kibizu) of Amami Ohshima island International conference

Mimura A, Toshima Y Suzuki Y, Yazaki S, Ohtsuki T, Ui S, Hyoudou F

The 3rd International Conference of Food Factors: Physiologic Functions and Disease Risk Reduction 2003.12

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Event date： 2003.12

Language：English Presentation type：Other
ゴマ脱脂粕に含まれるセサミノール配糖体の微生物発酵による遊離促進効果 Major achievement

大槻隆司,三村精男

第１８回日本ゴマ科学会大会 2003.11

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Event date： 2003.11

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
Development of novel water treatment system using bio-mat with microbial consortium of cyanobacteria and aerobic bacteria International conference

Mimura A, Takei R, Ohtsuki T, Ui S, Yazaki S

Marine Biotechnology Conference 2003 2003.9

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Event date： 2003.9

Language：English Presentation type：Oral presentation(general)
Utilization of palm oil mill fiber in situ bioactivator process by Chaetomium sp. nov. MS-017

Suyanto, Ohtsuki T, Yazaki S, Ui S, Mimura A

2003.9

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Event date： 2003.9

Language：English Presentation type：Oral presentation(general)
複合微生物系構築によるバイオマスからの微好気条件下水素生産 Major achievement

伊藤王人,大槻隆司,宇井定春,三村精男

平成１５年度日本生物工学会大会 2003.9

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Event date： 2003.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：熊本大学
白色腐朽菌によるフタロシアニン色素の脱色と分解浄化への応用

椙村佳奈,大槻隆司,宇井定春,三村精男

平成１５年度日本生物工学会大会 2003.9

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Event date： 2003.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：熊本大学
オイルパーム繊維の高温糸状菌培養による新規キシラナーゼ複合体の生産とその性質 Major achievement

大槻隆司,スヤント,矢崎伸一,宇井定春,三村精男

平成１５年度日本生物工学会大会 2003.9

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Event date： 2003.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：熊本大学
奄美サトウキビ、砂糖キビ酢のヒト骨髄性白血病細胞増殖抑制効果

鈴木賢洋,大槻隆司,矢崎伸一、宇井定春、兵藤文則、三村精男

平成１５年度日本生物工学会大会 2003.9

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Event date： 2003.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：熊本大学
新規酵素系構築大腸菌によるキラル体L-2,3-ブタンジオール生産

佐藤哲朗,田草川裕介,宇井定春,大槻隆司,三村精男、大熊盛也、工藤俊章

平成１５年度日本生物工学会大会 2003.9

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Event date： 2003.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：熊本大学
2,3-butanediol dehydrogenaseの立体基質特異性の変換

田草川裕介,宇井定春,大槻隆司,三村精男、栗栖源嗣、楠木正巳、大熊盛也、工藤俊章

平成１５年度日本生物工学会大会 2003.9

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Event date： 2003.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：熊本大学
Enhanced production of antioxidative sesaminol glucosides from sesame oil cake through fermentation by Bacillus circulans strain YUS-2 International conference

Ohtsuki T, Akiyama J, Shimoyama T, Yazaki S, Ui S, Mimura A

International Conference on Functional and Health Foods: Market, Technology and Health Benefit 2003.8

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Event date： 2003.8

Language：English Presentation type：Oral presentation(general)
Production of novel xylanosome by thermophilic fungus Chaetomium sp. nov. MS-017 on solid state fermentation of palm oil mill fiber International conference Major achievement

Ohtsuki T, Suyanto, Koesnandar, Ui S, Mimura A

2003 Annual Meeting of Indonesian Society for Microbiology 2003.8

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Event date： 2003.8

Language：English Presentation type：Oral presentation(general)
Development of in situ bioactivator process on aplm oil mill fiber by Chaetomium sp. nov. MS-017 International conference

Suyanto, Ohtsuki T, Subroto MA, Koesnandar, Ui S, Mimura A

2003 Annual Meeting of Indonesian Society for Microbiology 2003.8

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Event date： 2003.8

Language：English Presentation type：Oral presentation(general)
奄美サトウキビ、キビ酢、ソテツ発酵デンプン成分のヒト白血病細胞増殖抑制活性と活性酸素消去活性は、長寿食の根拠になるのか？

三村精男,加藤伸一,鈴木賢洋,矢崎伸一,大槻隆司,宇井定春、兵藤文則

第１０回日本がん予防研究会・第２６回日本がん疫学研究会 2003.6

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Event date： 2003.6

Language：Japanese Presentation type：Oral presentation(general)

Venue：北海道大学
担子菌が生産するマンガン非依存性ペルオキシダーゼ活性に対する不飽和脂肪酸の影響

溝口耕太郎,大槻隆司,宇井定春,三村精男

日本農芸化学会２００３年度大会 2003.4

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Event date： 2003.4

Language：Japanese Presentation type：Oral presentation(general)

Venue：日本大学生物資源学部
パームファイバーの固相発酵により生産される高温糸状菌キシラナーゼの性状 Major achievement

大槻隆司,スヤント,矢崎伸一,宇井定春,三村精男

日本農芸化学会２００３年度大会 2003.4

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Event date： 2003.4

Language：Japanese Presentation type：Oral presentation(general)

Venue：日本大学生物資源学部
奄美大島の蘇鉄発酵デンプンの白血病細胞増殖抑制効果

加藤伸一,矢崎伸一,大槻隆司,宇井定春、兵藤文則、三村精男

日本農芸化学会２００３年度大会 2003.4

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Event date： 2003.4

Language：Japanese Presentation type：Oral presentation(general)

Venue：日本大学生物資源学部
2,3-butanediol dehydrogenaseの立体基質特異性の変換

平成１５年度日本生物工学会大会 2003

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Language：Japanese
Utilization of palm oil mill fiber in situ bioactivator process by Chaetomium sp. nov. MS-017

平成１５年度日本生物工学会大会 2003

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Language：English Presentation type：Oral presentation(general)
Development of novel water treatment system using bio-mat with microbial consortium of cyanobacteria and aerobic bacteria

Marine Biotechnology Conference 2003 2003

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Language：English Presentation type：Oral presentation(general)
Development of in situ bioactivator process on aplm oil mill fiber by Chaetomium sp. nov. MS-017

2003 Annual Meeting of Indonesian Society for Microbiology 2003

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Language：English Presentation type：Oral presentation(general)
複合微生物系構築によるバイオマスからの微好気条件下水素生産

平成１５年度日本生物工学会大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
白色腐朽菌によるフタロシアニン色素の脱色と分解浄化への応用

平成１５年度日本生物工学会大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
新規酵素系構築大腸菌によるキラル体L-2,3-ブタンジオール生産

平成１５年度日本生物工学会大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
担子菌が生産するマンガン非依存性ペルオキシダーゼ活性に対する不飽和脂肪酸の影響

日本農芸化学会２００３年度大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
奄美大島の蘇鉄発酵デンプンの白血病細胞増殖抑制効果

日本農芸化学会２００３年度大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
奄美サトウキビ、砂糖キビ酢のヒト骨髄性白血病細胞増殖抑制効果

平成１５年度日本生物工学会大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
奄美サトウキビ、キビ酢、ソテツ発酵デンプン成分のヒト白血病細胞増殖抑制活性と活性酸素消去活性は、長寿食の根拠になるのか？

第１０回日本がん予防研究会・第２６回日本がん疫学研究会 2003

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Language：Japanese Presentation type：Oral presentation(general)
パームファイバーの固相発酵により生産される高温糸状菌キシラナーゼの性状

日本農芸化学会２００３年度大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
ゴマ脱脂粕に含まれるセサミノール配糖体の微生物発酵による遊離促進効果

第１８回日本ゴマ科学会大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
オイルパーム繊維の高温糸状菌培養による新規キシラナーゼ複合体の生産とその性質

平成１５年度日本生物工学会大会 2003

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Language：Japanese Presentation type：Oral presentation(general)
Production of novel xylanosome by thermophilic fungus Chaetomium sp. nov. MS-017 on solid state fermentation of palm oil mill fiber International coauthorship International conference

2003 Annual Meeting of Indonesian Society for Microbiology 2003

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Language：English Presentation type：Oral presentation(general)
Induction of apoptosis in human leukemia cells by a natural fermented sugar cane vinegar (Kibizu) of Amami Ohshima island International conference

The 3rd International Conference of Food Factors: Physiologic Functions and Disease Risk Reduction 2003

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Language：English Presentation type：Oral presentation(general)
Enhanced production of antioxidative sesaminol glucosides from sesame oil cake through fermentation by Bacillus circulans strain YUS-2 International conference

International Conference on Functional and Health Foods: Market, Technology and Health Benefit 2003

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Language：English Presentation type：Oral presentation(general)
Integrated development of biomass plantations for energy, and commodity and fine chemicals International conference

Mimura A, Suyanto, Ohtsuki T

15th International Congress of Chemical and Process Engineering 2002.8

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Event date： 2002.8

Language：English Presentation type：Oral presentation(general)
The integrated biomass utilization based on microbial biotechnology for sustainable development of oil palm plantations International conference

Suyanto, Ohtsuki T, Ui S< Koesnandar, Mimura A

2002 International Oil Palm Conference 2002.7

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Event date： 2002.7

Language：English Presentation type：Oral presentation(general)
海洋性ラン藻と微生物の共生系構築による海水浄化

武井良二,矢崎伸一,大槻隆司,宇井定春,三村精男

第６回マリンバイオテクノロジー学会大会 2002.5

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Event date： 2002.5

Language：Japanese Presentation type：Oral presentation(general)

Venue：東京農工大学
日向当帰（日本山人参）葉成分のヒト腫瘍細胞増殖抑制活性 Major achievement

姫地実加,大槻隆司,宇井定春,三村精男

日本農芸化学会2002年度大会 2002.3

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Event date： 2002.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：東北学院大学
奄美サトウキビ酢のヒト腫瘍細胞増殖抑制活性

鈴木賢洋,戸島洋平,姫地実加,矢崎伸一,保坂毅,大槻隆司,宇井定春、三村精男

日本農芸化学会2002年度大会 2002.3

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Event date： 2002.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：東北学院大学
The integrated biomass utilization based on microbial biotechnology for sustainable development of oil palm plantations International coauthorship International conference

2002 International Oil Palm Conference 2002

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Language：English Presentation type：Oral presentation(general)
Integrated development of biomass plantations for energy, and commodity and fine chemicals

15th International Congress of Chemical and Process Engineering 2002

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Language：English Presentation type：Oral presentation(general)
海洋性ラン藻と微生物の共生系構築による海水浄化

第６回マリンバイオテクノロジー学会大会 2002

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Language：Japanese Presentation type：Oral presentation(general)
日向当帰（日本山人参）葉成分のヒト腫瘍細胞増殖抑制活性

日本農芸化学会2002年度大会 2002

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Language：Japanese Presentation type：Oral presentation(general)
奄美サトウキビ酢のヒト腫瘍細胞増殖抑制活性

日本農芸化学会2002年度大会 2002

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Language：Japanese Presentation type：Oral presentation(general)
Decomposition of palm oil mill fiber by fungus MS-017 under higher temperature International conference

Mimura A, Ohtsuki T, Tsuchiya S, Ui S< Suyanto, Koesnandar

The Second Indonesian Biotechnology Conference 2001 2001.10

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Event date： 2001.10

Language：English Presentation type：Oral presentation(general)
コガネバナ培養細胞によるヒト骨肉腫及び白血病細胞致死物質の生成

姫地実加,田中美穂,矢崎伸一,大槻隆司,宇井定春、山本久子、田坂捷雄，三村精男

日本生物工学会平成１３年度大会 2001.9

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Event date： 2001.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
海洋性ラン藻とトリエタノールアミン分解微生物の共生系構築による海水浄化

武井良二,矢崎伸一,大槻隆司,宇井定春,三村精男

日本生物工学会平成１３年度大会 2001.9

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Event date： 2001.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
細菌における2,3-ブタンジオールサイクルの生理的意義

保坂毅,宇井定春,大槻隆司,三村精男

日本生物工学会平成１３年度大会 2001.9

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Event date： 2001.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
Enzymes decomposing palm oil mill fiber in fungus MS-017 under higher temperature

Suyanto, Yazaki S, Ohtsuki T, Ui S, Mimura A

2001.9

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Event date： 2001.9

Language：English Presentation type：Oral presentation(general)
稲わらを基質とした高温糸状菌による酵素生産の検討 Major achievement

大槻隆司,スヤント,矢崎伸一,宇井定春,三村精男

日本生物工学会平成１３年度大会 2001.9

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Event date： 2001.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
ゴマ油粕の微生物変換によるリグナン抗酸化性物質の生成

下山誉郎,兀下伸二,秋山純子,矢崎伸一,大槻隆司,広瀬裕子、宇井定春、三村精男

日本生物工学会平成１３年度大会 2001.9

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Event date： 2001.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
キメラ体2,3-ブタンジオールデヒドロゲナーゼの立体基質特異性

田草川裕介,宇井定春,大槻隆司,三村精男、栗栖源嗣，楠木正巳、大熊盛也、工藤俊章

日本生物工学会平成１３年度大会 2001.9

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Event date： 2001.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：山梨大学
The integrated biomass utilization based on microbial biotechnology for sustainable development of tropical plantations International conference

Mimura A, Ohtsuki T, Tsuchiya S, Ui S, Suyanto, Koesnandar

10th European Congress on Biotechnology 2001.7

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Event date： 2001.7

Language：English Presentation type：Oral presentation(general)
稲わらの固相発酵によるヘミセルロースの分解 Major achievement

大槻隆司,スヤント、土屋幸子、矢崎伸一、宇井定春、三村精男

日本農芸化学会2001年度大会 2001.3

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Event date： 2001.3

Language：Japanese Presentation type：Oral presentation(general)

Venue：立命館大学
The integrated biomass utilization based on microbial biotechnology for sustainable development of tropical plantations International coauthorship International conference

10th European Congress on Biotechnology 2001

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Language：English Presentation type：Oral presentation(general)
Enzymes decomposing palm oil mill fiber in fungus MS-017 under higher temperature

日本生物工学会平成１３年度大会 2001

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Language：English Presentation type：Oral presentation(general)
Decomposition of palm oil mill fiber by fungus MS-017 under higher temperature

The Second Indonesian Biotechnology Conference 2001 2001

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Language：English Presentation type：Oral presentation(general)
細菌における2,3-ブタンジオールサイクルの生理的意義

日本生物工学会平成１３年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
稲わらを基質とした高温糸状菌による酵素生産の検討

日本生物工学会平成１３年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
稲わらの固相発酵によるヘミセルロースの分解

日本農芸化学会2001年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
海洋性ラン藻とトリエタノールアミン分解微生物の共生系構築による海水浄化

日本生物工学会平成１３年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
ゴマ油粕の微生物変換によるリグナン抗酸化性物質の生成

日本生物工学会平成１３年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
コガネバナ培養細胞によるヒト骨肉腫及び白血病細胞致死物質の生成

日本生物工学会平成１３年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
キメラ体2,3-ブタンジオールデヒドロゲナーゼの立体基質特異性

日本生物工学会平成１３年度大会 2001

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Language：Japanese Presentation type：Oral presentation(general)
Novel process of water bioremediation with photosynthetic cyanobacterial consortium International conference

Mimura A, Kouno S, Iwai A, Yazaki S, Ohtsuki T, Ui S

Biotechnology 2000: The World Congress on Biotechnology 2000.9

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Event date： 2000.9

Language：English Presentation type：Oral presentation(general)

Venue：Berlin, Germany
Isolation and cultivation of fungi on palm oil mill fiber under higher temperature

Suyanto, Yazaki S, Ohtsuki T, Ui S, Mimura A

2000.8

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Event date： 2000.8

Language：English Presentation type：Oral presentation(general)
高温糸状菌による稲わらの効率的分解 Major achievement

大槻隆司、スヤント、土屋幸子、矢崎伸一、宇井定春、三村精男

日本生物工学会平成１２年度大会 2000.8

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Event date： 2000.8

Language：Japanese Presentation type：Oral presentation(general)

Venue：北海道大学
Composting of palm oil mill fiber by fungi under higher temperature International conference

Mimura A, Suyanto, Yazaki S, Ohtsuki T, Ui S

5th International Symposium on Environmental Biotechnology 2000.7

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Event date： 2000.7

Language：English Presentation type：Oral presentation(general)

Venue：Kyoto, Japan
海洋性ラン藻と微生物の共生系構築による海水浄化

三村精男、武井良二、米田聡、矢崎伸一、大槻隆司、宇井定春

第４回マリンバイオテクノロジー学会大会 2000.5

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Event date： 2000.5

Language：Japanese Presentation type：Poster presentation

Venue：香川県県民ホール
細菌における2,3-butanediol cycleの分布

保坂毅、宇井定春、水谷王称、大槻隆司、三村精男

日本農芸化学会2000年度大会 2000.4

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Event date： 2000.4

Language：Japanese Presentation type：Poster presentation

Venue：東京ビックサイト
温度感受性吸水性ハイドロゲルを用いたラン科幼植物の開放系培養

三村精男、三上綾子、大槻隆司、宇井定春、矢崎伸一、窪田真紀子、小保内康弘、向山武彦

日本農芸化学会2000年度大会 2000.4

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Event date： 2000.4

Language：Japanese Presentation type：Poster presentation

Venue：東京ビックサイト
形質転換大腸菌によるL(+)-2,3-ブタンジオール脱水素酵素の調製

田草川裕介、宇井定春、大槻隆司、三村精男、大熊盛也、工藤俊章

日本生物工学会平成１１年度大会 1999.9

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Event date： 1999.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：関西大学
ブドウプロトプラストの分裂阻害因子の分離同定

名取貴光、宇井定春、松原寿樹、小又理穂、奥田徹、大槻隆司、三村精男

日本生物工学会平成１１年度大会 1999.9

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Event date： 1999.9

Language：Japanese Presentation type：Oral presentation(general)

Venue：関西大学
Purification of flavonoid (wogonin) by suspension culture of Scutellaria baicalensis and its induction of apoptosis of human monocytic leukemia cells International conference

Mimura A, Fukasawa H, Tanaka M, Yazaki S, Ohtsuki T, Ui S

2000 Year Natural Products Research 1999.7

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Event date： 1999.7

Language：English Presentation type：Oral presentation(general)

Venue：Amsterdam, Netherlands
CD26/DPPIVによるSDF-1機能の負の制御 Major achievement

大槻隆司、細野治、小林弘、森本幾夫

第２８回日本免疫学会総会・学術集会 1998.12

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Event date： 1998.12

Language：Japanese Presentation type：Oral presentation(general)

Venue：神戸国際会議場・国際展示場
Pseudomonas hydrogenovoraのヒドロゲナーゼ遺伝子群の発現機構の解析 Major achievement

大槻隆司、藤岡豊文、高木美保、下坂誠、岡崎光雄

日本農芸化学会1997年度大会 1997.4

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Event date： 1997.4

Language：Japanese Presentation type：Oral presentation(general)

Venue：東京農業大学
Pseudomonas hydrogenovoraのヒドロゲナーゼ遺伝子群の構造と発現 Major achievement

大槻隆司、藤岡豊文、高木美保、下坂誠、岡崎光雄

日本生物工学会平成８年度大会 1996.10

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Event date： 1996.10

Language：Japanese Presentation type：Oral presentation(general)

Venue：名古屋大学
Pseudomonas hydrogenovoraのヒドロゲナーゼ遺伝子群の構造解析 Major achievement

大槻隆司、北幸弘、藤岡豊文、橋本大相、下坂誠、岡崎光雄

日本農芸化学会1996年度大会 1996

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Event date： 1996

Language：Japanese Presentation type：Oral presentation(general)
Pseudomonas hydrogenovoraのヒドロゲナーゼ構造遺伝子オペロンの解析 Major achievement

大槻隆司、北幸弘、藤岡豊文、下坂誠、岡崎光雄

日本農芸化学会1995年度大会 1995

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Event date： 1995

Language：Japanese Presentation type：Oral presentation(general)
Pseudomonas hydrogenovoraの膜結合型ヒドロゲナーゼ遺伝子の発現解析 Major achievement

大槻隆司、北幸弘、藤岡豊文、橋本大相、下坂誠、岡崎光雄

日本生物工学会平成７年度大会 1995

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Event date： 1995

Language：Japanese Presentation type：Oral presentation(general)
水素細菌Pseudomonas hydrogenovoraのヒドロゲナーゼ遺伝子の解析 Major achievement

大槻隆司、鈴木優子、北幸弘、下坂誠、岡崎光雄

日本農芸化学会1994年度大会 1994

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Event date： 1994

Language：Japanese Presentation type：Oral presentation(general)
水素細菌Pseudomonas hydrogenovoraの膜結合型ヒドロゲナーゼの発現調節遺伝子群の解析 Major achievement

大槻隆司、鈴木優子、北幸弘、藤岡豊文、下坂誠、岡崎光雄

日本生物工学会平成６年度大会 1994

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Event date： 1994

Language：Japanese Presentation type：Oral presentation(general)
水素細菌Pseudomonas hydrogenovoraのヒドロゲナーゼの遺伝子の解析 Major achievement

大槻隆司、鈴木優子、北幸弘、下坂誠、岡崎光雄

日本生物工学会平成５年度大会 1993

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Event date： 1993

Language：Japanese Presentation type：Oral presentation(general)

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Industrial Property Rights

セリ科植物の種子を高効率で発芽させる方法、そのためのキット及びその製造方法

大槻隆司

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Application no：特願2018-019985 Date applied：2018.2

Announcement no：特開2019-135937 Date announced：2019.8

Patent/Registration no：7076078 Date registered：2022.5

Country of applicant：Domestic
キノコ廃菌床を再利用した菌床

宇井定春,中島俊,大槻隆司,野田悟子

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Application no：2010-184318 Date applied：2010.8

Announcement no：2012-39948 Date announced：2012.3

Country of applicant：Domestic

Awards

The Best Poster Presentation Award of The 3rd Indonesian Biotechnology Conference

2004.12 Indonesia Society for Microbiology Production of xylanase complex and antifungal compounds by solid state fermentation on palm oil mill fiber using Chaetomium sp. nov. MS-017

Ohtsuki T, Suyanto, Itabashi T, Yazaki S, Ui S, Mimura A

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Award type：Award from international society, conference, symposium, etc. Country：Indonesia
Foundation, Oil and Fat Industry Kaikan Award for Technical Article

2004.2 Foundation, Oil and Fat Industry Kaikan

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Award type：Award from publisher, newspaper, foundation, etc. Country：Japan

Teaching Experience (On-campus)

Experiments in Biochemistry

2023Year
Bioengineering Experiment I

2023Year
Concepts of Integrated Applied Life Science

2023Year
Outline of Life Science Major achievement

2023Year
Outline of Biology Major achievement

2023Year
Research on Bioscience A

2023Year
Seminar on Bioscience A

2023Year
Environmental Microbiology and Bioresources Major achievement

2023Year
生命環境学演習Ａ Major achievement

2021Year Type of subject：Master's (Graduate School)
Basic Practice in Bioresources Management

2021Year
生命工学卒業論文 Major achievement

2021Year Type of subject：Professional education (undergraduate)
Basic Microbiology Major achievement

2021Year
生命工学研究室実習 Major achievement

2021Year Type of subject：Professional education (undergraduate)
Outline of Biology Major achievement

2021Year
Experiments in Molecular Biology Major achievement

2021Year Type of subject：Professional education (undergraduate)
Bioengineering Experiment II

2021Year Type of subject：Professional education (undergraduate)
Technical English

2021Year
Environmental Microbiology and Bioresources Major achievement

2021Year
統合応用生命科学特論

2021Year Type of subject：Dr. (Graduate School)
生命環境学研究Ａ Major achievement

2021Year Type of subject：Master's (Graduate School)
生命工学研究第二A Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学研究第一Ｂ Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学演習第一Ｂ Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学演習第一Ａ Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学演習第二A Major achievement

2017Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2017Year Type of subject：Master's (Graduate School)
Environmental microbiology andBioresources Major achievement

2016Year
生命工学演習第一Ａ Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学演習第一Ｂ Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学演習第二A Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学研究第一Ｂ Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学研究第二A Major achievement

2016Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2016Year Type of subject：Master's (Graduate School)
研究発表Ａ

2016Year
Utilization of MicrobialFunction

2016Year
Technical English II

2016Year
Bioengineering Experiment IV Major achievement

2016Year
Advanced Technology forUtilization of BiologicalFunction Major achievement

2016Year
Advanced Approach toBiological Informations Major achievement

2016Year
Biotechnology in PresentDaily Life

2016Year
Outline of Life Science Major achievement

2016Year
Bioengineering Experiment IV Major achievement

2015Year Type of subject：Professional education (undergraduate)
研究発表特論II Major achievement

2015Year Type of subject：Master's (Graduate School)
研究発表特論II Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学演習第一Ａ Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学演習第一Ｂ Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学演習第二A Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学研究第一B Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学研究第二A Major achievement

2015Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2015Year Type of subject：Master's (Graduate School)
生物利用工学特論 Major achievement

2015Year Type of subject：Dr. (Graduate School)
構造インフォマティクス特論 Major achievement

2015Year Type of subject：Master's (Graduate School)
生命環境基礎ゼミ Major achievement

2015Year Type of subject：Common education (undergraduate)
Graduation Thesis of Biotechnology Major achievement

2015Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise II Major achievement

2015Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise ⅠI Major achievement

2015Year Type of subject：Professional education (undergraduate)
Technical English II Major achievement

2015Year Type of subject：Professional education (undergraduate)
Introduction to Life Science Major achievement

2015Year Type of subject：Common education (undergraduate)
Bioengineering Experiment IV Major achievement

2014Year Type of subject：Professional education (undergraduate)
研究発表特論II Major achievement

2014Year Type of subject：Master's (Graduate School)
研究発表特論I Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学演習第一Ａ Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学演習第一Ｂ Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学演習第二A Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学研究第一B Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学研究第二A Major achievement

2014Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2014Year Type of subject：Master's (Graduate School)
生物利用工学特論 Major achievement

2014Year Type of subject：Dr. (Graduate School)
構造インフォマティクス特論 Major achievement

2014Year Type of subject：Master's (Graduate School)
生命環境基礎ゼミ Major achievement

2014Year Type of subject：Common education (undergraduate)
Graduation Thesis of Biotechnology Major achievement

2014Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise ⅠI Major achievement

2014Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise Ⅰ Major achievement

2014Year Type of subject：Professional education (undergraduate)
Technical English II Major achievement

2014Year Type of subject：Professional education (undergraduate)
Introduction to Life Science Major achievement

2014Year Type of subject：Common education (undergraduate)
Presentation II Major achievement

2013Year Type of subject：Master's (Graduate School)
Presentation I Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学演習第一B Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学演習第一A Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学演習第二A Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学研究第一B Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学研究第二Ａ Major achievement

2013Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2013Year Type of subject：Master's (Graduate School)
Informatics in Biostructure (Advanced) Major achievement

2013Year Type of subject：Master's (Graduate School)
Graduation Thesis of Biotechnology Major achievement

2013Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise ⅠI Major achievement

2013Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise Ⅰ Major achievement

2013Year Type of subject：Professional education (undergraduate)
Process Development in Bioengineering Major achievement

2013Year Type of subject：Professional education (undergraduate)
Technical English II Major achievement

2013Year Type of subject：Professional education (undergraduate)
Introduction to Life Science Major achievement

2013Year Type of subject：Common education (undergraduate)
Bioengineering Experiment IV Major achievement

2013Year Type of subject：Professional education (undergraduate)
生命環境基礎ゼミ Major achievement

2013Year Type of subject：Common education (undergraduate)
生命環境基礎ゼミ Major achievement

2012Year Type of subject：Common education (undergraduate)
Presentation I Major achievement

2012Year Type of subject：Master's (Graduate School)
Presentation II Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学演習第一Ａ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学演習第一Ｂ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学演習第二Ａ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学研究第一Ｂ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学研究第二Ａ Major achievement

2012Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2012Year Type of subject：Master's (Graduate School)
Informatics in Biostructure (Advanced) Major achievement

2012Year Type of subject：Master's (Graduate School)
Graduation Thesis of Biotechnology Major achievement

2012Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise ⅠI Major achievement

2012Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise Ⅰ Major achievement

2012Year Type of subject：Professional education (undergraduate)
Process Development in Bioengineering Major achievement

2012Year Type of subject：Professional education (undergraduate)
Technical English II Major achievement

2012Year Type of subject：Professional education (undergraduate)
現代生活とバイオテクノロジー Major achievement

2012Year Type of subject：Common education (undergraduate)
Exercise on Molecular Biology

2012Year Type of subject：Professional education (undergraduate)
Organic and Biological Chemistry II Major achievement

2012Year Type of subject：Professional education (undergraduate)
Bioengineering Experiment IV Major achievement

2012Year Type of subject：Professional education (undergraduate)
Utilization of Biological Functions (Advanced) Major achievement

2012Year Type of subject：Dr. (Graduate School)
Informatics in Biostructure (Advanced) Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学研究第二Ｂ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学研究第二Ａ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学研究第一Ｂ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学研究第一Ａ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学演習第二Ｂ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学演習第二Ａ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学演習第一Ｂ Major achievement

2011Year Type of subject：Master's (Graduate School)
生命工学演習第一Ａ Major achievement

2011Year Type of subject：Master's (Graduate School)
Presentation II Major achievement

2011Year Type of subject：Master's (Graduate School)
Presentation I Major achievement

2011Year Type of subject：Master's (Graduate School)
Graduation Thesis of Biotechnology Major achievement

2011Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise ⅠI Major achievement

2011Year Type of subject：Professional education (undergraduate)
Scientific Engilish Exercise Ⅰ Major achievement

2011Year Type of subject：Professional education (undergraduate)
Process Development in Bioengineering Major achievement

2011Year Type of subject：Professional education (undergraduate)
Technical English II Major achievement

2011Year Type of subject：Professional education (undergraduate)
現代生活とバイオテクノロジー Major achievement

2011Year Type of subject：Common education (undergraduate)
Exercise on Molecular Biology Major achievement

2011Year Type of subject：Professional education (undergraduate)
Organic and Biological Chemistry II Major achievement

2011Year Type of subject：Professional education (undergraduate)
Bioengineering Experiment IV Major achievement

2011Year Type of subject：Professional education (undergraduate)
生物有機化学第二 Major achievement

2010Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2010Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2010Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2009Year Type of subject：Professional education (undergraduate)
生物有機化学第二 Major achievement

2009Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2009Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2008Year Type of subject：Professional education (undergraduate)
生物工学実験Ⅳ Major achievement

2008Year Type of subject：Professional education (undergraduate)
生物有機化学第二 Major achievement

2008Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2007Year Type of subject：Professional education (undergraduate)
生物有機化学第二 Major achievement

2007Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2007Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2006Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2006Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2005Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2005Year Type of subject：Professional education (undergraduate)
分子生物学演習 Major achievement

2004Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2004Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2003Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2002Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2001Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

2000Year Type of subject：Professional education (undergraduate)
生物工学実験IV Major achievement

1999Year Type of subject：Professional education (undergraduate)

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Other educational achievements

紹介文：ブランチスピリット・東日本支部の生物工学教育活動 (1)　～学生発表討論会～. 日本生物工学会誌Vol.88, Issue 1, p.28-29

2010
著作：”自由研究は面白い”に誘う私の工夫～生徒の興味との接点を探す～、大槻隆司、日本語、教育雑誌「楽しい理科授業」、印刷中、明治図書出版（株）（東京）

2005

International Exchange and International Contribution

2010 Type：International, regional exchange, exchange of students and Japanese students

山梨県教育委員会平成２２年度やまなし若者地域活性化プロジェクト推進事業「理想のまちプロジェクト」「ハナミズキ酵母でパンを焼こう」イベント講演会「みんなの生活とバイオテクノロジー」講師

Professional Memberships

Indian Society for Education and Environment

2009.4
Japanese Society of Water Treatment Biology

2009.4
Japanese Society for Immunology

1998.10 - 2010.9
Japanese Society for Bioscience, Biotechnology and Agrochemistry

1994
The Society for Biotechnology, Japan

1993
JAPANESE SOCIETY OF WATER TREATMENT BIOLOGY
日本生物工学会
日本農芸化学会

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Committee Memberships

日本生物工学会東日本支部支部委員

2023.4 - 2024.3

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Committee type：Society
日本生物工学会代議員

2023.4 - 2024.3

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Committee type：Society
日本生物工学会東日本支部監事

2023.4 - 2024.3

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Committee type：Society
日本生物工学会代議員

2022.4 - 2023.3

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Committee type：Society
日本生物工学会東日本支部監事

2022.4 - 2023.3

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Committee type：Society
日本生物工学会東日本支部支部委員

2022.4 - 2023.3

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Committee type：Society
日本生物工学会東日本支部支部委員

2021.4 - 2022.3

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Committee type：Society
日本生物工学会代議員

2021.4 - 2022.3

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Committee type：Society
日本生物工学会東日本支部監事

2021.4 - 2022.3

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Committee type：Society
日本生物工学会代議員

2020.4 - 2021.3

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Committee type：Society
日本生物工学会東日本支部支部委員

2020.4 - 2021.3

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Committee type：Society
日本生物工学会東日本支部監事

2020.4 - 2021.3

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Committee type：Society
日本農芸化学会参与

2012.3 - 2015

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Committee type：Society
日本農芸化学会代議員

2009.4
日本生物工学会代議員

2005.4
日本生物工学会東日本支部委員

2004.4

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