Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press  

ABSTRACT

Weed, an abundant biomass, is considered unsuitable as a raw material for methane production. There are few reports on the anaerobic digestion of weeds without the addition of other organic wastes. To solve this problem, a methane-producing microbial community with weed as a sole feedstock was established. This study mainly focused on the degree of contribution between water-soluble and -insoluble fractions of the weed to methane production; thus, methane production from both fractions was tested separately. Methane production after 80-day batch cultures with whole weed, water-soluble and water-insoluble fractions was 184.5, 96.8 and 26.5 NmL g−1 dry matter (DM), respectively. The results of 16S rRNA gene amplicon sequence analysis revealed that Proteiniphilum saccharofermentans and several Methanobacterium species commonly dominated all cultures, whereas the population dynamics of minor species differed in every culture. Moreover, the remixed culture of microbial communities adapted to water-soluble and -insoluble fractions recovered methane production (252.4 NmL g−1 DM). Based on these results, it can be strongly inferred that colocalizing the minor species in water-soluble and -insoluble fractions is important for effective methane production.

DOI： 10.1093/femsle/fnab015

Other Link： http://academic.oup.com/femsle/article-pdf/368/4/fnab015/36450867/fnab015.pdf

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI AG  

Biogasification by methane fermentation is an important and effective way to utilize beverage wastes. Beverage wastes are good feedstocks for methane fermentation because of their richness in sugars and proteins, although overacidification and inhibition of methane production caused by high substrate loading often become problematic. This study investigated changes in microbial communities in the overacidification state of the thermophilic methane fermentation process with beverage waste by establishing a simulated batch culture. We assessed 20 mL-scale batch cultures using a simulant beverage waste mixture (SBWM) with different amounts of addition; high cumulative methane production was achieved by adding 5 mL of SBWM (11358 mg—chemical oxygen demand—COD/L of organic loading), and overacidification was observed by adding 10 mL of SBWM (22715 mg—COD/L of organic loading). The results of 16S rRNA amplicon sequence analysis using nanopore sequencer suggested that Coprothermobacter proteolyticus, Defluviitoga tunisiensis, Acetomicrobium mobile, and Thermosediminibacter oceani were predominantly involved in hydrolysis/acidogenesis/acetogenesis processes, whereas Methanothrix soehngenii was the major acetotrophic methane producer. A comparison of microbial population between the methane-producing cultures and overacidification cultures revealed characteristic population changes especially in some minor species under 0.2% of population. We concluded that careful monitoring of population changes of the minor species is a potential indicator for prediction of overacidification.

DOI： 10.3390/ijerph17207514

Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (commerce magazine)   Publisher：化学工業社  

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.5182/jaie.27.27

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DOI： 10.2521/jswtb.51.37

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：BENTHAM SCIENCE PUBL LTD  

A chimeric (2S, 3S)-butanediol dehydrogenase (cLBDH) was engineered to have the strict (S)-configuration specificity of the (2S, 3S)-BDH (BsLBDH) derived from Brevibacterium saccharolyticum as well as the enzymatic stability of the (2R, 3S)-BDH (KpMBDH) from Klebsiella pneumonia by swapping the domains of two native BDHs. However, while cLBDH possesses the stability, it lacks the specificity. In order to assist in the design a BDH having strict substrate specificity, an X-ray structural analysis of a cLBDH crystal was conducted at 1.58 angstrom. The results obtained show some readily apparent differences around the active sites of cLBDH and BsLBDH. Based on this structural information, a novel (2S, 3S)-BDH having a preferred specificity was developed by introducing a V254L mutation into cLBDH. The influence of this mutation on the stability of cLBDH was not evaluated. Nevertheless, the technique described herein is an effective method for the production of a tailor-made BDH.

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A polyphasic approach was used to determine the taxonomic position of actinomycete strain R1-NS-10(T), which was isolated from a sample of strawberry root rhizosphere obtained from Hokuto, Yamanashi, Japan. Strain R1-NS-10(T) was Gram-staining-positive and aerobic, and formed brownish-white aerial mycelia and grayish-brown substrate mycelia on ISP-2 medium. The strain grew in the presence of 0-5% (w/v) NaCl and optimally grew without NaCl. The strain grew at pH 5-8, and the optimum for growth was pH 7. The optimal growth temperature was 30 degrees C, but the strain grew at 5-37 degrees C. Whole-cell hydrolysates of strain R1-NS-10(T) contained A(2)pm, galactose, mannose and rhamnose. The predominant menaquinones were MK-9(H-6) and MK-9(H-8). The major cellular fatty acids were anteiso-C-15:0 and iso-C-16:0. Comparative 16S rRNA gene sequence analysis revealed that strain R1-NS-10(T) was most closely related to Streptomyces prunicolor NBRC 13075(T) (99.4%). The draft genome sequences of both strains were determined for characterization of genome sequence-related parameters such as average nucleotide identity (ANI) and the diversity of secondary metabolite biosynthetic gene clusters. DNA-DNA hybridization (DDH) and ANI values for both strains were below the species delineation cutoff, and differences in physiological and biochemical characteristics differentiated strain R1-NS-10T from its closest phylogenetic relative. On the basis of these data, we propose that strain R1-NS-10(T) (= NBRC 108812(T) = KCTC 29186(T)) should be classified as the type strain of a novel Streptomyces species named Streptomyces hokutonensis sp. nov.

DOI： 10.1038/ja.2014.20

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

A domain-chimeric L-2,3-butanediol dehydrogenase (chimera L-BDH), which was designed to possess both the S-configuration specificity of L-BDH and the stability of meso-BDH, was constructed by exchanging the respective domains of these two BDHs. However, chimera L-BDH possessed a lower enzymatic function than expected based on the two original enzymes. To elucidate the causes of the decreased stability and substrate specificity, crystallization of the protein was performed. Chimera L-BDH was purified to homogeneity via ammonium sulfate fractionation and three column-chromatography steps, and was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group C222(1), diffracted synchrotron radiation to 1.58 angstrom resolution and were most likely to contain two molecules in the asymmetric unit.

DOI： 10.1107/S2053230X13032755

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Union of Crystallography  

A domain-chimeric l-2,3-butanediol dehydrogenase (chimera l-BDH), which was designed to possess both the S-configuration specificity of l-BDH and the stability of meso-BDH, was constructed by exchanging the respective domains of these two BDHs. However, chimera l-BDH possessed a lower enzymatic function than expected based on the two original enzymes. To elucidate the causes of the decreased stability and substrate specificity, crystallization of the protein was performed. Chimera l-BDH was purified to homogeneity via ammonium sulfate fractionation and three column-chromatography steps, and was crystallized using the hanging-drop vapour-diffusion method. The crystals belonged to space group C2221, diffracted synchrotron radiation to 1.58 Å resolution and were most likely to contain two molecules in the asymmetric unit. © 2014 International Union of Crystallography All rights reserved.

DOI： 10.1107/S2053230X13032755

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Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

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Language：English   Publishing type：Research paper (bulletin of university, research institution)  

Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：日本生物工学会  

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Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：BENTHAM SCIENCE PUBL LTD  

The development of a stable L-BDH chimera was attempted by exchanging whole domains between two native structural analogs, L-BDH and meso-BDH, because the S-configuration specificity of L-BDH is valuable from the standpoint of its application but its activity is unstable, whereas meso-BDH is stable. The domain chimeras obtained indicated that the leaf-like structures constituting three domains were likely to be mainly associated with chiral recognition, and the fourth domain, the basic domain, is likely to be mainly associated with enzyme stability. A combination of the leaf domains of L-BDH and the basic domain of meso-BDH attained a sufficient level of practical use as an artificial L-BDH chimera, because the resulting enzyme had both stability and S-configuration specificity. However, the levels of stability and specificity were slightly lower than those of the respective enzymes from which they were derived.

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

2,3-Butanediol dehydrogenase (BDH) catalyzes the NAD-dependent redox reaction between acetoin and 2,3-butanediol. There are three types of homologous BDH, each stereospecific for both substrate and product. To establish how these homologous enzymes possess differential stereospecificities, we determined the crystal structure of L-BDH with a bound inhibitor at 2.0 angstrom. Comparison with the inhibitor binding mode of meso-BDH highlights the role of a hydrogen-bond from a conserved Trp residue(192). Site-directed mutagenesis of three active site residues of meso-BDH, including Trp(190), which corresponds to Trp(192) of L-BDH, converted its stereospecificity to that of L-BDH. This result confirms the importance of conserved residues in modifying the stereospeci. city of homologous enzymes. (C) 2009 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

DOI： 10.1016/j.febslet.2009.11.068

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Language：Japanese   Publishing type：(MISC) Other article   Publisher：日本生物工学会  

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：INST TECNOLOGIA PARANA  

Production of baicalein, baicalin and wogonin by liquid culture of Scutellaria baicalensis cells derived from the plant root was studied. The maximum production obtained were 119 mg/L of baicalein at two week, 1372 mg/L of baicalin at eight week, and 14 mg/L of wogonin at two week. In addition, the production of baicalin was drastically increased to 1000 mg/L level at 3-week culture, and the extremely high production rate (339 mg/L.week) was obtained. In the comparison of total antioxidative activities among baicalein, baicalin and wogonin, evaluated by thiocyanate method, it was suggested that the location of hydroxyl groups both at 5- and 6-position contributed to enhancement of radical scavenging activity, and/or methoxylation at 8-position diminished the activity. The possibility of utilizing these flavonoids for natural antioxidants and medicine is also discussed.

DOI： 10.1590/S1516-89132009000200005

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Indian Society for Education and Environment  

In this study, we successfully developed activated sludge (AS) adapted to a very high concentration (3 g/L) of phenol. A careful stepwiseadaptation process was considered important in the development of this AS. In addition, we found that processed lignite (PL) accelerated the removal of phenol which might lead to change in the dominant members of the microbial community. Phenol is an abundant industrial toxic contaminant in wastewater treatment processes.

DOI： 10.17485/ijst/2009/v2i10/30708

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Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：日本生物工学会  

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Methanol extract from cultured Scutellaria baicalensis cells inhibited the proliferation of human monocytic leukemia cell line THP-1 and human osteogenic sarcoma cell line HOS. The inhibitory effects of baicalin, baicalein and wogonin, the three major flavonoids contained in the extract, were studied. It should be noted that wogonin did not show the inhibitory effect on human fetal lung normal diploid cell line TIG-1, as compared to the inhibition observed in cancer cells. Physiological analyses in THP-1 cells showed that wogonin induced cell cycle arrest at G(2)/M phase and apoptosis. This is the first report discovering a cancer-specific apoptosis-inducing activity of wogonin. (c) 2006 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.canlet.2006.01.011

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Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：BLACKWELL PUBLISHING LTD  

Aims: A novel xylanolytic multienzyme complex of the aerobic thermophilic fungus Chaetomium sp. nov. MS-017 was produced on palm oil mill fibre (POMF) and partially characterized.
Methods and Results: The assay of the extracellular enzymes of Chaetomium sp. nov. MS-017 on POMF in solid-state fermentation revealed cellulolytic, pectinolytic and extremely high xylanolytic activities. The protein was purified by Sephadex G-200 column chromatography. The SDS-PAGE demonstrated that the purified protein is a complex with at least five xylanolytic, four cellulolytic and eight pectinolytic components. The characterization of the complex at various temperatures showed that the reactivity and stability of the complex are not lost up to 60degreesC. In addition, the complex was very stable in a wide range of pH (3-9) and at high concentrations (10 mM) of cations and EDTA. The major products of xylan hydrolysis by the purified complex were determined to be xylobiose and xylotriose by thin-layer chromatography.
Conclusion: Chaetomium sp. nov. MS-017 preferentially produces a xylanolytic multienzyme complex on POMF in solid-state fermentation.
Significance and Impact of the Study: This is the first report on the xylanolytic multienzyme complex produced by an aerobic thermophilic fungus.

DOI： 10.1111/j.1472-765X.2004.01644.x

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Naturally fermented vinegar such as Kibizu (sugar cane vinegar in Amami Ohshima, Japan), Kurozu (black rice vinegar in Kagoshima. Japan), Kouzu (black rice vinegar in China) and red wine vinegar in Italy had potent radical-scavenging activity analyzed by DPPH method. For the elucidation of food factor for cancer prevention contained in naturally fermented vinegar. the induction of apoptosis in human leukemia cell HL-60 was investigated with sugar cane vinegar Kibizu.
Fraction eluted by 40% methanol from Amberlite XAD 2 chromatography of sugar cane vinegar showed potent radical scavenging activity. The fraction also showed the activity repressing growth of typical human leukemia cells such as HL-60, THP-1 Molt-4, U-937, Jurkat, Raji and K-562. On the other hand, the fraction did not have any growth inhibition activity against human fetal lung cell TIG-1. The most potent radical-scavenging activity and the growth repression activity of the leukemia cell were observed in the same chromatographic fraction of methanol 40%.
From cell sorting FACS analyses, electron microscopic observations and cytochemical staining of chromatin and nuclear segments in human leukemia cell HL-60 treated with the active fraction, it was concluded that apoptosis was induced in the leukemia cell by the fraction of sugar cane vinegar and resulted in the repression of growth of the human leukemia cells.
Chromatographic fraction of sugar cane juice eluted by 20% methanol showed potent activities of radical-scavenging and growth repression of HL-60. These results led us the consideration that active components in sugar cane juice could be converted to more lipophilic compounds with activity to induce apoptosis in HL-60 by microbial fermentation with yeast and acetic acid bacteria.

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Aims: A metabolic pathway for L-2,3-butanediol ( BD) as the main product has not yet been found. To rectify this situation, we attempted to produce L-BD from diacetyl (DA) by producing simultaneous expression of diacetyl reductase (DAR) and L-2,3-butanediol dehydrogenase (BDH) using transgenic bacteria, Escherichia coli JM109/ pBUD-comb.
Methods and Results: The meso-BDH of Klebsiella pneumoniae was used for its DAR activity to convert DA to L-acetoin (AC) and the L-BDH of Brevibacterium saccharolyticum was used to reduce L-AC to L-BD. The respective gene coding each enzyme was connected in tandem to the MCS of pFLAG-CTC (pBUD-comb). The divided addition of DA as a source, addition of 2% glucose, and the combination of static and shaking culture was effective for the production.
Conclusions: L-BD ( 2200 mg l(-1)) was generated from 3000 mg l(-1) added of DA, which corresponded to a 73% conversion rate. Meso-BD as a by-product was mixed by 2% at most.
Significance and Impact of the Study: An enzyme system for converting DA to L- BD was constructed with a view to using DA-producing bacteria in the future.

DOI： 10.1111/j.1472-765X.2004.01622.x

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：TAYLOR & FRANCIS LTD  

Bacillus circulans strain YUS-2 was isolated as the strongest antioxidant-producer in fermentation of sesame oil cake (SOC, defatted residue yielded from sesame seed oil production). Two major strong antioxidants from fermented SOC were purified and identified as known sesaminol triglucoside and sesaminol diglucoside, however, our results demonstrated that the fermentation process with B. circulans YUS-2 was highly effective to gain the extraction efficiency of the sesaminol glucosides.

DOI： 10.1271/bbb.67.2304

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPRINGER-VERLAG  

Palm-oil mill fiber (POMF) is a fibrous, natural hard material discharged in enormous amounts from palm-oil mills in tropical plantations: therefore, research to find microorganisms that decompose POMF was conducted. As the result of screening, a new thermophilic fungus, Chaetomium sp. nov. MS-017, exhibiting rapid growth on POMF was isolated from rotted wood. Based on partial characterization of the decomposition of POMF, it was shown that MS-017 preferentially assimilates poly saccharides, especially hemicelluloses such as xylan. A preliminary composting study indicated that MS-017 produced 855 g of decomposed product from 1,000 g of intact POMF in 12 days under optimized solid-culture conditions. The decomposition rate of POMF was 23% (w/w), and the cell yield calculated from consumed POMF was as high as 36% (w/w). These results indicate that MS-017 has a very high potential to decompose POMF and that it is suitable for economical production of compost to recycle by-product biomass from oil-palm plantations.

DOI： 10.1007/s00253-002-1125-2

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Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN  

Acetylacetoin synthase (AACSase) and acetylacetoin reductase (AACRase) are representative enzymes of the 2,3-butanediol cycle. After examining their induction conditions in various bacteria, the former was induced by acetoin and the latter by glucose. All strains carrying AACSase also had AACRase, but the reverse was not true. Therefore, AACSase indicates the existence of the cycle. Acetylacetoin (AAC) accumulation or the ratio of 2,3-butanediol isomer formed also indicated the presence of the cycle in bacteria. This cycle is present in some strains and not in others even for those belonging to the same species. The cycle was not always associated with the representative 2,3-butanediol-producing bacteria or bacterial sporogenesis as reported previously.

DOI： 10.1263/jbb.93.248

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The L-2,3-butanediol dehydrogenase produced in E coti JM109/pLBD2-CTC was purified by 5 steps. The molecular mass of this enzyme was estimated at 110 kDa and the subunit was measured to be 30 kDa. The L-BDH had some differences from the BDHs from other sources in substrate specificity, pI value, pH stability, effects of divalent cations, and organic acids.

DOI： 10.1271/bbb.65.1876

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SOC BIOSCIENCE BIOENGINEERING JAPAN  

A 1.4-kbp DNA fragment, including the NADH-linked acetylacetoin reductase/2,3-butanediol dehydrogenase (AACRII/BDH) gene from the chromosomal DNA of Bacillus cereus YUF-4, was cloned in Escherichia coli DH5 alpha after its insertion into pUC119, and the resulting plasmid was named pAACRII119. The AACRII/BDH gene had an open reading frame consisting of 1047 bp encoding 349 amino acids. The enzyme exhibited not only AACR activity, but also BDH activity. However, the gene was not located in a 2,3-butanediol (BD) operon, as is the case in the BDH gene of Klebsiella pneumoniae and that of K. terrigena. In addition, there was no BD-cycle-related enzyme gene in the region surrounding the AACRII/BDH gene. The AACR and BDH activities in E. coli DH5 alpha /pAACRII119 were 200-fold higher than those in the original B. cereus YUF-4. The characteristics of the AACRII/BDH from E. coli DH 5 alpha /pAACRII119 are similar to those of the AACRH/BDH from B. cereus YUF-4. The AACRH/BDH was considered to belong to the NAD(P)- and zinc-dependent long-chain alcohol dehydrogenase (group I ADH) family on the basis of the following distinctive characteristics: it possessed 14 strictly conserved residues of microbial group I ADH and consisted of about 350 amino acids. The enzymatic and genetic characteristics of AACRII/BDH were completely different from those of BDHs belonging to the short-chain dehydrogenase/reductase family. These findings indicated that the AACRII/BDH could be considered a new type of BDH.

DOI： 10.1263/jbb.91.539

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

The L-2,3-butanediol dehydrogenase (L-BDH) gene of Brevibacterium saccharolyticum was strongly expressed in Escherichia coli using the tac promoter. However, the stereospecificity of the resulting L-BDH was reduced. The region upstream of the meso-BDH gene of Klebsiella pneumoniae was also involved in the expression of the B. saccharolyticum gene. However, in this case, the resulting L-BDH exhibited more stable stereospecificity. A stereospecificity recognition region was located within the rear sequence (Hpa I site, carboxy terminal) of the BDH open reading frame. Using a transformed strain of E. coli, the conversion of L-acetoin (L-AC), in the commercially available racemic mixture of AC, to L-2,3-butanediol (L-BD) was attempted. As a result, 0.37% L-BD was formed from 1% AC added to the culture.

DOI： 10.1046/j.1472-765X.2001.00869.x

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Authorship：Lead author   Language：English   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Authorship：Lead author   Language：English   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：ELSEVIER SCI IRELAND LTD  

Acquired immune deficiency syndrome (AIDS:) is an incurable disease at present and so many efforts to conquer this disease are being made around the world. In studies of human immunodeficiency virus (HIV) infection and the disease progression. it has been reported that T cells expressing CD26 are preferentially infected and depleted in HIV-infected individuals. CD26 is a widely distributed 110 kDa cell-surface glycoprotein with known dipeptidyl peptidase IV (DPPIV) activity in its extracellular domain. This ectoenzyme is capable of cleaving N-terminal dipeptides from polypeptides with either proline or alanine residues in the penultimate position. On human T cells. CD26 exhibits the co-stimulatory function and plays an important role in immune response via its ability to bind adenosine deaminase (ADA) and association with CD45. Recent studies have been stripping the veil from over the relationship between CD26 and HIV infection. Susceptibility of cells to HIV infection is correlated with CD26 expression. and HIV transactivator Tat and envelope protein gp120 are reported to interact with CD26. These observations indicate that CD26 is closely involved in HIV cell entry and that CD26-mediatod T cell immune response is suppressed. In addition. it has been demonstrated that the anti-HIV and chemotactic activities of RANTES (regulated on activation. normal T cell expressed and secreted) and stromal cell-derived factor-1 (SDF-1) are controlled with the DPPIV activity of CD26. Thus. the regulation of the function of chemokines by CD26/DPPIV appears to be essential fbr lymphocyte trafficking and infectivity of HIV strains. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

DOI： 10.1016/S0923-1811(99)00081-X

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO(+) memory subset and a CD45RA(+) naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4, The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral Ca4 T cells and 1D4(+) CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1D4 antigen is human CD43, Transfection of CHO-leu cells, which stably express human CD43, with core 2 beta-1,6-N-acetylslucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only In 1D4(+) T cells but not in 1D4(-) T cells, implying that the 1D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1D4 was completely abolished when cells were treated with neuraminidase, while there remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans, Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT; whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305, Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO(+) memory subset of CD4 T cells.

DOI： 10.1093/intimm/11.2.259

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PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：OXFORD UNIV PRESS  

Human CD4 T cells can be divided into two functionally distinct subsets: a CD45RO(+) memory subset and a CD45RA(+) naive subset. In an attempt to identify novel cell surface molecules on these cells, we have developed a mAb, anti-1D4, The antigen defined by anti-1D4 was preferentially expressed on the memory subset of freshly isolated peripheral Ca4 T cells and 1D4(+) CD4 T cells functionally corresponded to memory T cells. Retrovirus-mediated expression cloning revealed that the 1D4 antigen is human CD43, Transfection of CHO-leu cells, which stably express human CD43, with core 2 beta-1,6-N-acetylslucosaminyltransferase (C2GnT) conferred expression of the 1D4 antigen and mRNA of C2GnT was detected by RT-PCR only In 1D4(+) T cells but not in 1D4(-) T cells, implying that the 1D4 antigen is composed of core 2-containing O-glycans on CD43. Reactivity with anti-1D4 was completely abolished when cells were treated with neuraminidase, while there remained weak binding of anti-T305, a previously described mAb which also reacts with CD43 modified with core 2-containing O-glycans, Moreover, anti-1D4 markedly reacted with NIH-3T3 cells expressing human CD43 and low levels of endogenous C2GnT; whereas anti-T305 reacted slightly. These results indicate that the 1D4 antigen is distinct from the epitope defined by anti-T305 and anti-1D4 is a more sensitive probe to detect core 2-containing O-glycans than anti-T305, Taken together, our results indicate that core 2-containing O-glycans, whose expression can easily be detected with anti-1D4, are preferentially expressed in the CD45RO(+) memory subset of CD4 T cells.

DOI： 10.1093/intimm/11.2.259

Web of Science

PubMed

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

Stromal cell-derived factor 1 alpha (SDF-1 alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this studg, we show that SDF-1 alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory activity of SDF-1 alpha against HIV infection disappeared, Moreover, the chemotactic activity of SDF-1 alpha also disappeared specifically by DPPIV activity of recombinant soluble CD26, These results suggested that dissemination of T-tropic HIV strains in vivo may be facilitated by CD26/DPPIV via inactivation of functional SDF-1 alpha. (C) 1998 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(98)00763-7

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

Stromal cell-derived factor 1 alpha (SDF-1 alpha) is a chemokine that has been shown to prevent infection of T-tropic HIV strains and is a possible substrate of CD26/dipeptidyl peptidase IV (DPPIV). In this studg, we show that SDF-1 alpha was cleaved at the N-terminal region by CD26/DPPIV and as a result the inhibitory activity of SDF-1 alpha against HIV infection disappeared, Moreover, the chemotactic activity of SDF-1 alpha also disappeared specifically by DPPIV activity of recombinant soluble CD26, These results suggested that dissemination of T-tropic HIV strains in vivo may be facilitated by CD26/DPPIV via inactivation of functional SDF-1 alpha. (C) 1998 Federation of European Biochemical Societies.

DOI： 10.1016/S0014-5793(98)00763-7

Web of Science

PubMed

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：TAYLOR & FRANCIS LTD  

Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative sigma(54)-type promoter sequences (P-hup1, P-hup2, and P-hup3). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that P-hup1 and P-hup3 would be essential for a transcriptional initiation. It was also found that the region upstream of P-hup sequences was concerned with regulation of transcription.

DOI： 10.1271/bbb.61.1986

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：TAYLOR & FRANCIS LTD  

Expression of a membrane-bound hydrogenase of the hydrogen-utilizing bacterium Pseudomonas hydrogenovora was was induced specifically in autotrophic condition. Dot blot and primer extension analysis showed that the transcription of the hydrogenase gene started from the region upstream of a hydrogenase structural gene, hupS, which contained three putative sigma(54)-type promoter sequences (P-hup1, P-hup2, and P-hup3). The lacZ-fusion analysis of the hupS-upstream region combined with site-directed mutagenesis showed that P-hup1 and P-hup3 would be essential for a transcriptional initiation. It was also found that the region upstream of P-hup sequences was concerned with regulation of transcription.

DOI： 10.1271/bbb.61.1986

Web of Science

PubMed

Language：Japanese   Publishing type：Doctoral Thesis  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

The hydrogenase gene cluster containing nine genes (hupSLCDFGHIJ) was identified by sequencing of an 8.8-kb DNA region from Pseudomonas hydrogenovora. To investigate the function of the hupC gene product, we isolated a hupC-null mutant (HID3) of P. hydrogenovora by introducing an in-frame deletion into the hupC. The mutant, HID3, could not grow autotrophically but retained half the level of hydrogenase activity of the wild-type strain. Results of the oxygen consumption test and Western blot analysis revealed that the hupC gene product is a b-type cytochrome but not involved in the hydrogenase maturation process.

DOI： 10.1016/S0378-1097(97)00108-0

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

The hydrogenase gene cluster containing nine genes (hupSLCDFGHIJ) was identified by sequencing of an 8.8-kb DNA region from Pseudomonas hydrogenovora. To investigate the function of the hupC gene product, we isolated a hupC-null mutant (HID3) of P. hydrogenovora by introducing an in-frame deletion into the hupC. The mutant, HID3, could not grow autotrophically but retained half the level of hydrogenase activity of the wild-type strain. Results of the oxygen consumption test and Western blot analysis revealed that the hupC gene product is a b-type cytochrome but not involved in the hydrogenase maturation process.

DOI： 10.1016/S0378-1097(97)00108-0

Web of Science

PubMed

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

The membrane-bound hydrogenase (Hdg)-encoding structural genes were isolated from the hydrogen-utilizing bacterium Pseudomonas hydrogenovora (Ph). Nucleotide sequence analysis revealed two genes, hupS and hupL. The hupS gene encoded a 363-amino-acid (aa) polypeptide (40.4 kDa). The deduced aa sequence contained a putative 43-aa leader peptide sequence. The hupL gene started at 55 bp downstream from the stop codon of hupS and encoded a 622-aa polypeptide (69.3 kDa). The disruption of Ph hupS resulted in the loss of Hdg activity.

DOI： 10.1016/0378-1119(95)00580-4

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

The membrane-bound hydrogenase (Hdg)-encoding structural genes were isolated from the hydrogen-utilizing bacterium Pseudomonas hydrogenovora (Ph). Nucleotide sequence analysis revealed two genes, hupS and hupL. The hupS gene encoded a 363-amino-acid (aa) polypeptide (40.4 kDa). The deduced aa sequence contained a putative 43-aa leader peptide sequence. The hupL gene started at 55 bp downstream from the stop codon of hupS and encoded a 622-aa polypeptide (69.3 kDa). The disruption of Ph hupS resulted in the loss of Hdg activity.

DOI： 10.1016/0378-1119(95)00580-4

Web of Science

PubMed

Language：Japanese   Publishing type：Master Thesis  

Language：Japanese  

Responsible for pages：364-370   Language：English  

Responsible for pages：371-381   Language：English  

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Language：English  

Utilization of tropical biomass from plantations to produce useful materials for sustainable development of Indonesia. II. Development of in-field composting system by solid state fermentation process with thermophilic fungus for sustainable prosperity of oil palm plantations in Indonesia

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Venue：熊本大学  

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Venue：熊本大学  

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Venue：熊本大学  

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Venue：山梨大学  

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Venue：山梨大学  

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