Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.2139/ssrn.4632693

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：MYU K.K.  

DOI： 10.18494/sam4570

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MYU K.K.  

DOI： 10.18494/sam4493

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

CiNii Research

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：(株)北隆館  

電気化学顕微鏡は細胞近傍の対象分子濃度分布を2次元イメージとして得られる技術である。微小電極を用いて酸化/還元に基づく電流値から濃度情報を得て,電極の位置情報から濃度分布のマッピングを行う。電気化学顕微鏡には大きく分けて2つの方式がある。ひとつは,走査型電気化学顕微鏡であり,1細胞レベルの高解像度イメージングが可能である。もう1つは微小電極アレイであり,秒以下の時間分解能でリアルタイミングイメージングが可能である。我々は,高解像度・高速を両立する方法として,バイポーラ電気化学顕微鏡の研究を行っている。本稿ではこれらの3つの手法について概説する。(著者抄録)

Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Authorship：Lead author   Language：Japanese   Publishing type：Research paper (research society, symposium materials, etc.)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jallcom.2023.169844

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.biosx.2023.100312

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

The Limulus amebocyte lysate (LAL) reaction-based assay, the most commonly used endotoxin detection method, requires a skilled technician. In this study, to develop an easy-to-use and highly sensitive endotoxin sensor, we created an electrochemical endotoxin sensor by using an interdigitated array electrode (IDAE) device with advantages of amplifiable signals via redox cycling and portability. We added Boc-Leu-Gly-Arg-p-aminophenol (LGR-pAP) as an electrochemical substrate for an LAL reaction and detected p-aminophenol (pAP) released from LGR-pAP as a product of an endotoxin-induced LAL reaction via an IDAE device. The IDAE device showed a great redox cycling efficiency of 79.8%, and a 4.79-fold signal amplification rate. Then, we confirmed that pAP was detectable in the presence of LGR-pAP through chronoamperometry with the potential of the anode stepped from -0.3 to 0.5 V vs. Ag/AgCl while the cathode was biased at -0.3 V vs. Ag/AgCl. Then, we performed an endotoxin assay by using the IDAE device. Our endotoxin sensor detected as low as 0.7 and 1.0 endotoxin unit/L after the LAL reaction for 1 h and 45 min, respectively, and these data were within the cut-off value for ultrapure dialysis fluid. Therefore, our highly sensitive endotoxin sensor is useful for ensuring medical safety.

DOI： 10.3390/mi14020327

PubMed

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been garnered increasing for its rapid worldwide spread. Each country had implemented city-wide lockdowns and immigration regulations to prevent the spread of the infection, resulting in severe economic consequences. Materials and technologies that monitor environmental conditions and wirelessly communicate such information to people are thus gaining considerable attention as a countermeasure. This study investigated the dynamic characteristics of batteryless magnetostrictive alloys for energy harvesting to detect human coronavirus 229E (HCoV-229E). Light and thin magnetostrictive Fe-Co/Ni clad plate with rectification, direct current (DC) voltage storage capacitor, and wireless information transmission circuits were developed for this purpose. The power consumption was reduced by improving the energy storage circuit, and the magnetostrictive clad plate under bending vibration stored a DC voltage of 1.9 V and wirelessly transmitted a signal to a personal computer once every 5 min and 10 s under bias magnetic fields of 0 and 10 mT, respectively. Then, on the clad plate surface, a novel CD13 biorecognition layer was immobilized using a self-assembled monolayer of -COOH groups, thus forming an amide bond with -NH2 groups for the detection of HCoV-229E. A bending vibration test demonstrated the resonance frequency changes because of HCoV-229E binding. The fluorescence signal demonstrated that HCoV-229E could be successfully detected. Thus, because HCoV-229E changed the dynamic characteristics of this plate, the CD13-modified magnetostrictive clad plate could detect HCoV-229E from the interval of wireless communication time. Therefore, a monitoring system that transmits/detects the presence of human coronavirus without batteries will be realized soon.

DOI： 10.1016/j.sna.2022.114052

PubMed

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1016/j.sna.2022.114052

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

Here, we report a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode. Endotoxin and factor XIa were used as the label molecules for the immunoassay of two types of analytes to induce the LAL and coagulation cascade reactions, respectively, when each corresponding analyte existed in the sample solution. In addition to the signal amplification by the cascade reactions, we employed redox cycling in a nanoscale gap to achieve a highly sensitive assay. The nanoscale-gap electrode amplifies the amperometric signals from p-aminophenol liberated from artificial substrates in the final steps of the cascade reactions. First, the cross reaction between the LAL and coagulation cascade reactions was investigated. The results indicated that these cascade reactions did not efficiently proceed in a single solution owing to the cross reaction. Therefore, we selected to induce two cascade reactions in different solutions by bisecting the beads after the immunocomplex formation on the beads. The cross reactions of factor XIa with the LAL cascade reaction and of endotoxin with the coagulation cascade reaction were investigated. The effects of these cross reactions were revealed to be negligible by bisecting the beads before inducing the cascade reactions. Finally, a dual immunoassay for goat and human immunoglobulin G was performed, for which the limits of detection were 70 pg/mL (470 fmol/L) and 1.0 ng/mL (6.6 pmol/L), respectively. Thus, our dual immunoassay provides a sensitive platform for clinical diagnosis requiring detection of multiple analytes.

DOI： 10.1021/acs.analchem.2c03921

PubMed

Authorship：Lead author   Language：Japanese   Publishing type：Research paper (research society, symposium materials, etc.)  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

Here, we report a highly sensitive immunoassay for human immunoglobulin G (IgG) that uses signal amplification of the coagulation cascade. Z-Phe-Pro-Lys-p-nitroaniline (FPK-pNA) was used as a substrate for thrombin activation in the last step of the coagulation cascade. During the coagulation cascade, pNA is liberated from FPK-pNA and can be detected electrochemically. Using square wave voltammetry with a glassy carbon electrode, we demonstrated that pNA can be quantified in a solution modeling the coagulation cascade prepared by mixing FPK-pNA and pNA. Characterization of the reactivity of thrombin toward FPK-pNA revealed that thrombin efficiently reacted with FPK-pNA. Subsequent characterization of factor XIa activity of factor XIa-labeled antibody revealed that factor XIa was not inactivated during labeling. Finally, a coagulation cascade-based immunoassay for human IgG was performed using a factor XIa-labeled antibody on magnetic beads. The limit of detection for human IgG was 5.0 pg/mL (33 fM) indicating that the coagulation cascade can amplify the immunoassay sensitivity compared to immunoassay using a thrombin-labeled antibody as a condition without a coagulation cascade. Coagulation cascade-based immunoassay was also highly selective. In the near future, we will report a highly sensitive immunoassay for the simultaneous detection of multiple analytes using a coagulation cascade-based immunoassay and Limulus amebocyte lysate reaction-based immunoassay we previously reported.

DOI： 10.1021/acs.analchem.2c02241

PubMed

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.sna.2022.113814

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society  

In this study, a carbon paste filling method was proposed as a simple strategy for fabricating high-density bipolar electrode (BPE) arrays for bipolar electrochemical microscopy (BEM). High spatiotemporal resolution imaging was achieved using the fabricated BPE array. BEM, which is an emerging microscopic system in recent years, achieves label-free and high spatiotemporal resolution imaging of molecular distributions using high-density BPE arrays and electrochemiluminescence (ECL) signals. We devised a simple method to fabricate a BPE array by filling a porous plate with carbon paste and succeeded in fabricating a high-density BPE array (15 μm pitch). After a detailed observation of the surface of the BPE array using a scanning electron microscope, the basic electrochemical and ECL emission characteristics were evaluated using potassium ferricyanide solution as a sample solution. Moreover, inflow imaging of the sample molecules was conducted to evaluate the imaging ability of the prepared BPE array. In addition, Prussian Blue containing carbon ink was applied to the sample solution side of the BPE array to provide catalytic activity to hydrogen peroxide, and the quantification and inflow imaging of hydrogen peroxide by ECL signals was achieved. This simple fabrication method of the BPE array can accelerate the research and development of BEM. Furthermore, hydrogen peroxide imaging by BEM is an important milestone for achieving bioimaging with high spatiotemporal resolution such as biomolecule imaging using enzymes.

DOI： 10.1021/acs.analchem.1c05354

PubMed

Authorship：Lead author   Language：Japanese   Publishing type：Research paper (research society, symposium materials, etc.)  

Language：Japanese   Publishing type：Research paper (research society, symposium materials, etc.)  

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Analytical Chemistry  

DOI： 10.2116/bunsekikagaku.71.109

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MYU K.K.  

DOI： 10.18494/sam3745

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

Imaging systems using closed bipolar electrode (cBPE) arrays and electrochemiluminescence (ECL) have attracted great attention in recent years as a 2D imaging platform with high spatiotemporal resolution. However, the fabrication techniques for cBPE arrays involve complicated procedures. Therefore, a new fabrication scheme enabling the mass production of cBPE arrays with high precision, reproducibility, and yield, is desired. Here, the use of a versatile and scalable thermal drawing process as a novel fabrication method for fiber-based cBPEs with feature sizes down to micro-/nanoscales is proposed. First, a single-electrode fiber consisting of a carbon-based composite as the electrode material is produced by thermal drawing. The fundamental electrical properties of the single-electrode fiber are characterized, and its applicability to the cBPE-ECL system is demonstrated. A multielectrode fiber is fabricated by subjecting a bundle of 104 single-electrode fibers to thermal drawing. Its usability as a cBPE array for ECL imaging is confirmed with a functional rate of 99%. Further the multielectrode fiber, utilizing the principle of thermal drawing, for magnified electrochemical imaging is tapered. This work establishes a novel mass-production method for cBPE arrays, as well as a proof of concept for magnified electrochemical imaging using a thermally-drawn electrode array fiber.

DOI： 10.1002/admt.202101066

Web of Science

Authorship：Lead author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Language：Japanese   Publisher：The Polarographic Society of Japan  

DOI： 10.5189/revpolarography.67.83

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

Here, we report a highly sensitive and easy-to-use electrochemical immunoassay using a dual-signal amplification strategy of redox cycling within a nanoscale gap and the Limulus amebocyte lysate (LAL) cascade reaction. After the antigen-antibody reaction using an endotoxin-labeled antibody, the LAL reaction was induced by endotoxin as the first signal amplification. Subsequently, p-aminophenol (pAP) liberated from peptideconjugated pAP (Boc-Leu-Gly-Arg-pAP; LGR-pAP), at the last step of the LAL reaction, was detected with redox cycling within a nanoscale gap using a fabricated device as the second signal amplification. First, an electrochemical immunoassay for goat immunoglobulin G (IgG), used as a model analyte, was performed with an endotoxin-labeled antibody using a 96-well plate. The results indicated that the endotoxin-labeled antibody was usable for immunoassays. However, a high background was observed. To decrease the background and increase the efficiency of the reactions, magnetic beads in an endotoxin-free test tube were used for the immunosorbent assay. After optimization of the condition, goat IgG was successfully detected with single-signal amplification of the cascade reaction at concentrations as low as 10 pg/mL (67 fM) using a Au disc electrode. Finally, an immunoassay for goat IgG on magnetic beads using a redox cycling-inducing device was performed. With the dual-signal amplification strategies of redox cycling within a nanoscale gap and LAL reactions, the detection limit was 70 fg/mL (470 aM). Our highly sensitive and easy-to-use immunoassay strategy provides a platform for clinical diagnosis to detect early-stage infections.

DOI： 10.1021/acsanm.1c02837

Web of Science

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/celc.202101037

Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1002/celc.202101037

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

A 2D imaging system using closed bipolar electrodes (cBPEs) and electrochemiluminescence (ECL) is expected to realize high spatio-temporal resolution imaging. However, imaging of the distributions of molecules that cause an oxidation reaction on the electrode surface was not achieved by the cBPE/ECL system because the conventionally used luminophore can generate ECL only by an oxidation reaction. In this paper, we propose to implement a cathodic luminophore, [Ru(bpy)(3)]Cl-2/glutathione disulfide (GSSG) producing ECL by reduction reaction into the cBPE/ECL system to enable detection and 2D imaging of dopamine, which is an oxidizable redox species and an important neurotransmitter. The applicability of [Ru(bpy)(3)]Cl-2/GSSG for dopamine detection using a single cBPE was shown and its electrochemical properties were discussed. In addition, the demonstration of the 2D imaging of dopamine was performed using a cBPE array. These results show the potential to greatly expand the applicability of detection and imaging systems using cBPE/ECL.

DOI： 10.1002/celc.202100675

Web of Science

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/celc.202100675

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Electrochemical Society of Japan  

In this work, we report a simple electrochemical sensor that detects proteinuria upon immersion into a sample. We immobilized peptidases in conjunction with glutamate oxidase (GluOx) on a Prussian bluemodified glassy carbon (PB-GC) electrode. In the sensor, albumin, which is a major protein in urine, was degraded by peptidases to produce glutamate that was detected by GluOx on PB-GC. Initially, we validated the strategy for the detection of proteinuria using glutamate sensing. We quantified the amount of glutamate produced by degrading human serum albumin with HCI and carboxypeptidase A (CPA). The results indicated that an increase in the enzyme degradation rate was necessary for daily proteinuria sensing that needs to be completed within 1 h. Therefore, we investigated the use of endopeptidase in conjunction with CPA. The combination of proteinase K and CPA liberated glutamate from albumin at 17.5 times higher comparing to solely CPA. The subsequent glutamate assay using peptidase-modified GluOxPB-GC electrode did not exhibit changes in the electrochemical signal with increases in the glutamate concentration, because peptidase degraded the GluOx on the modified electrode. To prevent this, GluOx was encapsulated in ZIF-8. Glutamate was successfully detected using peptidase and GluOx encapsulated in the ZIF-8-modified PB-GC (peptidase-ZIF-8/GluOx-PB-GC) electrode. Finally, an albumin assay was performed using the peptidase-ZIF-8/GluOx-PB-GC electrode, wherein albumin was detected with a sensitivity of 0.1 mg/mL within 30min. Our simple and easy-to-use method for albumin detection provides a viable sensor for daily urinalysis. (C) The Author(s) 2021. Published by ECSJ.

DOI： 10.5796/electrochemistry.21-00058

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

Bipolar electrode-based (BPE-based) electrochromic devices have garnered increasing attention in the past decade. These BPE-based electrochromic devices have been used for analytical health monitoring, point-of-care (POC) diagnostics, and chemical sensing. In this review, we highlight recent progress made regarding BPE-based electrochromic devices constructed for these analytical applications. Various, available electrochromic materials are summarized in the first section, after which the different device types (e. g., paper-based and self-powered) are discussed. Biological- and chemical-based analytical demonstrations of these devices are then reviewed. Finally, we conclude this review with a perspective on the future developments of BPE-based electrochromic devices in analytical applications.

DOI： 10.1002/elan.202100153

Web of Science

Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (commerce magazine)   Publisher：CMC Publishing  

Language：Japanese  

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC ANALYTICAL CHEMISTRY  

We have estimated the effect of a chemical reagent based on the oxygen consumption and the motion of zebrafish embryos by using an LSI-based amperometric sensor array device (BioLSI). Bio-LSI with 400 gold microelectrodes in a 6 mmx6 mm region enabled us to visualize the distribution of the concentration of redox species as images. We applied -0.2 V vs. Ag/AgCl to all electrodes of a Bio-LSI chip to obtain the distribution of the oxygen concentrations in the presence of zebrafish embryos. The motions of the embryos were observed and traced by a CMOS camera. The reduction currents, obtained at the electrodes immediately under the embryos, are smaller than that obtained at the electrodes without the embryos. When the embryos moved to the position above other electrodes, the positions of electrodes with the low reduction current synchronized to the position of the embryos. This was due to the consumption of oxygen by the respiration of embryos and the inhibition of the diffusion of oxygen to the electrodes. When a solution containing 2,4-dinitrophenol (DNP), that is an uncoupler of oxidative phosphorylation, was added, the reduction current drastically decreased and then gradually returned to the original level. The active motions of the embryos coupled with the decrease of the reduction current were observed immediately after adding DNP, while no motion was observed after turning toward original level. Embryos would transiently enhance the consumption of oxygen by respiration due to the compensation of its depression of ATP synthesis, and then be led to death. The present system based on the reduction currents of oxygen and the motions of embryos would provide simple and high-throughput toxicity tests for various chemical agents.

Web of Science

Authorship：Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

DOI： 0.5796/denkikagaku.20-FE0026

Authorship：Lead author   Language：English   Publishing type：(MISC) Summary of the papers read (international conference)  

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：(MISC) Summary of the papers read (international conference)  

DOI： 10.1149/MA2020-02442811mtgabs

Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

DOI： 0.5796/denkikagaku.20-FE0026

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

<p>In this study, we developed a bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to...</p>

DOI： 10.1039/d0an00912a

Web of Science

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：MDPI  

Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devic

DOI： 10.3390/mi11050530

Web of Science

PubMed

Authorship：Lead author   Language：English   Publishing type：(MISC) Summary of the papers read (international conference)  

Language：English  

DOI： 10.1149/MA2020-01332372mtgabs

Language：Japanese   Publishing type：Research paper (research society, symposium materials, etc.)  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Chemical Society of Japan  

Kallikrein is a serine protease which is involved in blood pressure regulation and is overexpressed in primary aldosteronism. We hypothesized that quick quantification of this enzyme could represent a valuable diagnostic technique. Towards this aim, we demonstrate the use of an electrochemical approach to detect and quantify kallikrein for the first time. We designed the tripeptide Pro-Phe-Arg-pMA as a substrate for kallikrein and analyzed the enzyme concentration by measuring the release of the product, p-methoxyaniline (pMA), from kallikrein-mediated hydrolysis. Released pMA was detected by electrochemical measurement with a detection limit of 0.01 units/mL. The fit of the analysis of released pMA by chronoamperometry with Michaelis-Menten curves indicates that this technique offers quantitative detection.

DOI： 10.1246/cl.190729

Web of Science

Language：Japanese  

J-GLOBAL

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

The light-driven splitting of water to oxygen (O2) is catalyzed by a protein-bound tetra-manganese penta-oxygen calcium (Mn4O5Ca) cluster in Photosystem II. In the current study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to perform two-dimensional imaging of light-induced O2 evolution from spinach leaves. The employed Bio-LSI chip consists of 400 sensor electrodes with a pitch of 250 μm for fast electrochemical imaging. Spinach leaves were illuminated to varying intensities of white light (400-700 nm) which induced oxygen evolution and subsequent electrochemical images were collected using the Bio-LSI chip. Bio-LSI images clearly showed the dose-dependent effects of the light-induced oxygen release from spinach leaves which was then significantly suppressed in the presence of urea-type herbicide 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). Our results clearly suggest that light-induced oxygen evolution can be monitored using the chip and suggesting that the Bio-LSI is a promising tool for real-time imaging. To the best of our knowledge, this report is the first to describe electrochemical imaging of light-induced O2 evolution using LSI-based amperometric sensors in plants.

DOI： 10.1038/s41598-019-48561-y

PubMed

Other Link： http://www.nature.com/articles/s41598-019-48561-y

Authorship：Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japan Society for Analytical Chemistry  

We report a rapid and simple method to detect endotoxin using a nanopore sensor based on the Coulter principle. An ion current momentary decreases when an endotoxin micelle translocates through the nanopore at a tip of the glass capillary with the voltage biased across the pore. Quantitative detection is available by counting pulsed currents appear within a limited period. First, we characterized the nanopores of fabricated glass capillaries using a scanning electron microscope. The size of the pore at the tip of the glass capillary was 260 nm. Next, endotoxin assays using a nanopipette were performed. An Ag/AgCl wire inserted into a glass capillary filled with phosphate buffer saline (PBS) containing endotoxin was biased at 1 V versus another Ag/AgCl wire inserted in a bulk solution containing endotoxin-free PBS. No pulsed current was observed during 4 min of monitoring when the endotoxin concentrations in the glass capillary were below 100 Endotoxin Unit (EU) L-1.When 500- 10000 EU L-1 was in the glass capillary, pulsed currents were observed with an increase of the pulse height and pulse frequency with increasing of the endotoxin concentration. This is because the size of the endotoxin micelles as well as the micelle concentration were increased with the increase of the endotoxin concentration. The fact that pulses appeared far less frequently than expected indicates that only large micelles associated with each other were observed as a pulsed current. Although the current detection limit is 500 EU L-1. optimization of the pore diameter will make it possible to detect even smaller micelles, resulting in an increased the sensitivity. This method is expected to be applied to use as a rapid and simple sensor to detect endotoxin, especially in the continuous monitoring of dialysis solution.

DOI： 10.2116/bunsekikagaku.68.575

Web of Science

Language：English   Publisher：IEEE  

DOI： 10.1109/TRANSDUCERS.2019.8808677

Web of Science

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Royal Society of Chemistry (RSC)  

<p>A highly sensitive endotoxin sensor and novel analytical principle using diffusion coefficient difference was developed using a nanocavity device.</p>

DOI： 10.1039/C9AN00478E

Web of Science

PubMed

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)  

Language：English  

In this study, we developed a novel electrochemical imaging system using a closed bipolar electrode (cBPE) with electrochemiluminescence (ECL) detecting system to realize the high spatio-temporal resolution imaging. This novel imaging system can obtain high spatial resolution images because each cBPEs need no connecting lines, which severely limited the spatial resolution of the conventional electrochemical images obtained by the micro-electrode array method. We successfully applied the system to monitor the spreading activity of potassium ferricyanide solution in high spatio-temporal resolution.

Scopus

Language：Japanese  

J-GLOBAL

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme-modified large-scale integration (LSI)-based amperometric devices, referred to “Bio-LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer-modified electrodes. The Os-HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme-modified Bio-LSI.

DOI： 10.1002/elan.201800386

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1002/elan.201800386

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

© 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme-modified large-scale integration (LSI)-based amperometric devices, referred to “Bio-LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer-modified electrodes. The Os-HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme-modified Bio-LSI.

DOI： 10.1002/elan.201881201

Web of Science

Scopus

Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese  

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

A novel liquid-junction-removed reference electrode system was developed for simultaneous multiplex amperometry using a closed bipolar electrode. The liquid junction between the reference cell and the sample cell was replaced by a bipolar electrode to ensure electron conductivity in lieu of ionic conductivity in conventional systems. Under the condition where the redox reaction of the analyte at the bipolar electrode in the sample cell was a limiting process, voltammetric and amperometric measurements were performed for quantitative detection of the analytes. After characterization of the system, a chip-type device was fabricated with six sample cells and one reference cell. Simultaneous determination of ferrocene methanol was conducted in six solutions in the 0.1-0.5 mM range. This is the first demonstration of the simultaneous multiplex determination of analytes with a liquid-junction-removed system. Although the potential control problem (which is different to the liquid potential problem of conventional liquid junction) still remains because of the increase of the solid-liquid interface, this system is expected to solve some problems associated with the liquid junction of the reference electrode, including sample contamination by chloride ions and the complicated process of fabricating a reference electrode on a chip.

DOI： 10.1002/celc.201800536

Web of Science

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1002/celc.201800895

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Inc.  

Reference electrodes (REs) play an essential role in electrochemical measurements. REs require liquid junctions to allow for ion conductivity between the RE and the sample solution. However, a liquid junction causes unwanted ion leakage into the analyte and clogging inducing reference potential inaccuracy. In this study, we report a liquid-junction-free amperometric probe sensor with an integrated RE, based on a closed bipolar electrode (cBPE) system. The liquid junction of the RE was replaced by the cBPE, which provides electrical conductivity instead of ionic conductivity between the inner solution of the RE and the sample solution. Since the solutions were in two separate cells, undesired contamination was completely prevented. We successfully conducted voltammetric and amperometric quantitative detection of 0.1–0.5 mM ferrocenemethanol using this system. Although its uses are limited, the present probe sensor with integrated liquid-junction-free reference electrode provides a feasible alternative for amperometric electrochemical measurements.

DOI： 10.1016/j.elecom.2018.06.006

Web of Science

Scopus

Language：English   Publishing type：(MISC) Other article   Publisher：WILEY-V C H VERLAG GMBH  

The front cover artwork is provided by Prof. K. Y. Inoue, Prof. T. Matsue, and Mr. T. Iwama from Tohoku University, Sendai (Japan). The image shows an electrochemical measuring device with six sample cells and one reference cell. Simultaneous multiplex amperometry and voltammetry are available with integrated reference system without a liquid junction.

DOI： 10.1002/celc.201800894

Web of Science

Language：Japanese   Publisher：(公社)日本分析化学会  

Language：Japanese   Publisher：(公社)日本分析化学会  

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Institute of Electrical Engineers of Japan  

In this research, enzyme electrode sensor for measuring low concentration lactate was fabricated. In our previous work, microperfusion needle to measure concentration of lactate in subepidermal tissue have been developed. Using this microperfusion needle, lactate concentration of perfusate was about one percent of lactate concentration of blood. In order to measure this low concentration of lactate, enzyme electrode sensor using printed enzyme was fabricated. Au electrodes were patterned on the glass substrate. Ag nanometal ink was printed on reference electrode. Os-HRP polymer was printed by pasting needle type printer and lactate oxidase was printed by inkjet printer on working electrode. Addition agents which do not inactivate enzymes as wetting agent and viscosity modifier were selected. A flow channel was pasted on fabricated electrodes to measure concentration changes continuously. Fabricated sensor had high sensitivity and could measure low concentration lactate (0.01-0.25 mM). In addition, fabricated sensor was not affected by interfering substances.

DOI： 10.1541/ieejsmas.138.231

Scopus

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：CHEMICAL SOC JAPAN  

© 2018 The Chemical Society of Japan. In this study, we developed a new chemical imaging platform that uses a closed bipolar electrode (cBPE) array for electrochemiluminescence (ECL) detection. The cBPE array was successfully applied to use in the chemical imaging of molecular distributions by injection and a self-induced redox cycling. The results show the possibility of high-resolution chemical imaging using a cBPE array.

DOI： 10.1246/cl.180303

Web of Science

Scopus

Language：Japanese  

J-GLOBAL

Language：Japanese  

J-GLOBAL

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ESG  

We report nitrogen doping diamond-like carbon film synthesis by a new technique called neutral beam enhanced chemical vapor deposition. The deposition is processed in UV/electron damage-free environment, and the substrate temperature is controlled at room temperature. The film structure was investigated by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The N/C ratio is 0.26, and doped nitrogen atoms exist in forms of amine and inside the aromatic ring, which helps increase film electrical conductivity. Cyclic voltammograms were carried out and the results indicate the DLC: N film qualified as electroanalysis sensor material.

DOI： 10.20964/2018.02.11

Web of Science

Scopus

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

Multiplexed bioimaging systems have triggered the development of effective assays, contributing new biological information. Although electrochemical imaging is beneficial for quantitative analysis in real time, monitoring multiple cell functions is difficult. We have developed a novel electrochemical imaging system, herein, using a large-scale integration (LSI)based amperometric device for detecting multiple biomolecules simultaneously. This system is designated as an electrochemicolor imaging system in which the current signals from two different types of biomolecules are depicted as a multicolor electrochemical image. The mode-selectable function of the 400-electrode device enables the imaging system and two different potentials can be independently applied to the selected electrodes. The imaging system is successfully applied for detecting multiple cell functions of the embryonic stem (ES) cell and the rat pheochromocytoma (PC12) cell aggregates. To the best of our knowledge, this is the first time that a real-time electrochemical mapping technique for multiple electroactive species, simultaneously, has been reported. The imaging system is a promising bioanalytical method for ex

DOI： 10.1021/acs.analchem.7b03042

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

Motion tracking of microorganisms is useful to investigate the effects of chemical or physical stimulation on their biological functions. Herein, we describe a novel electrochemical imaging method for motion tracking of microorganisms using a large-scale integration (LSI)-based amperometric device. The device consists of 400 electrochemical sensors with a pitch of 250 mm. A convection flow caused by the motion of microorganisms supplies redox species to the sensors and increases their electrochemical responses. Thus, the flow is converted to electrochemical signals, enabling the electrochemical motion tracking of the microorganisms. As a proof of concept, capillary vibration was monitored. Finally, the method was applied to monitoring the motion of Daphnia magna. The motions of these microorganisms were clearly tracked based on the electrochemical oxidation of [Fe(CN)(6)](4-) and reduction of O-2.

DOI： 10.1002/anie.201701541

Web of Science

PubMed

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

This manuscript reports on a novel substrate for the amperometric detection of cellular apoptosis, Asp-Glu-Val-Asp-p-methoxyaniline (DEVD-pMA). This substrate showed electrochemical inertia from -0.2 to 0.7V versus Ag/AgCl in phosphate-buffered saline (PBS), but was cleaved by caspase-3, releasing pMA, which was detected with cyclic voltammetry and chronoamperometry. Compared with p-aminophenol (pAP), pMA showed lower toxicity to the human hepatocellular carcinoma cell line (HepG2) and higher stability in PBS. By using this substrate, we successfully detected caspase-3 in the range of 0.31-2.5x10(-2) units/mL and cellular apoptosis in HepG2 cells by using simple amperometry without cell lysis. This work will help to develop a simple method for the electrochemical monitoring of apoptosis, which can be applied in biological research and drug development.

DOI： 10.1002/celc.201600700

Web of Science

Language：Japanese  

Language：Japanese  

Language：Japanese  

J-GLOBAL

Language：Japanese  

J-GLOBAL

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE BV  

Nitrogen-doped graphene is favored as a catalyst for oxygen reduction reaction (ORR) over rare metals. However, the effects of bonding state, nitrogen doped site and defects on catalytic conversion are still unclear. Here, we investigate oxygen reduction reaction using nitrogen-doped graphene with selective bonding state through pyridinic and graphitic nitrogen selective approaches. Both types show ORR activity and the catalytic reaction is clarified to be a four electron reaction path. Graphitic nitrogen with a low level of defects is found superior from the viewpoint of using single graphene sheet for the ORR application. Our investigation provides useful information for various applications using doped graphene. (C) 2016 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.cplett.2016.10.061

Web of Science

Language：English   Publishing type：Research paper (international conference proceedings)  

Language：English  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-V C H VERLAG GMBH  

Electrochemical imaging is an excellent technique to characterize an activity of biomaterials, such as enzymes and cells. Large scale integration-based amperometric sensor (Bio-LSI) has been developed for the simultaneous and continuous detection of the concentration distribution of redox species generated by reactions of biomolecules. In this study, the Bio-LSI system was demonstrated to be applicable for simultaneous detection of different analytes in multiple specimens. The multiple specimens containing human immunoglobulin G (hIgG) and mouse IgG (mIgG) were introduced into each channel of the upper substrate across the antibody lines for hIgG and mIgG on the lower substrate. Hydrogen peroxide generated by the enzyme reaction of glucose oxidase captured at intersections was simultaneously detected by 400 microelectrodes of a Bio-LSI chip. The oxidation current increased with increasing the concentrations of hIgG, which can be detected in the range of 0.01-1.0 mu g mL(-1). Simultaneous detection of hIgG and mIgG in multiple specimens was achieved by using line patterns of both antibodies. Therefore, the presence of different target molecules in the multiple samples would be quant

DOI： 10.1002/biot.201500559

Web of Science

PubMed

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE INC  

A liquid-junction-free electrochemical system has been developed for substitutional stripping voltammetry (SSV). SSV is a type of stripping analysis that changes the redox current to metal deposition. Generally, SSV requires a liquid junction to maintain electrical conductivity between separated cells. In this report, a closed bipolar electrode system(cBPES) was used to perform SSV without a liquid junction. In this system, electrical conductivity between separated cells was maintained using driving electrodes inserted into each cell. We demonstrated quantification of p-aminophenol (pAP) using a liquid-junction-free system for SSV. We investigated the effect of electrode area ratio on the BPE and the concentration ratio of redox species present in each cell. Then, we calibrated the pAP. A linear relationship between pAP concentration and SSV electrical charge was successfully obtained using our liquid-junction-free system, based on cBPES. This novel analytical system is a promising method for the fabrication of a compact SSV sensor for highly sensitive detections. (C) 2016 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.elecom.2016.02.014

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELECTROCHEMICAL SOC JAPAN  

We here report the electrochemical imaging of cell adhesion using a large-scale integration (LSI)-based amperometric device, called a Bio-LSI device. The device consists of 400 sensor electrodes arranged with a pitch of 250 pm. The device surface was modified with collagen to assist in the culture of MCF-7 cells and promote their adhesion. The cells disturb the electrochemical reaction of redox mediators, allowing the electrochemical signal to be used to evaluate cell adhesion at the single-cell level. This approach was applied to a cell detachment test. The results show that the Bio-LSI device is a promising tool for single-cell analysis. (C) The Electrochemical Society of Japan, All rights reserved.

DOI： 10.5796/electrochemistry.84.364

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELECTROCHEMICAL SOC JAPAN  

Oxygen consumption rate (OCR) of mouse embryoid body (EB) was measured repeatedly in glucose-depleted medium or PBS solution. Non-invasive nature of the scanning electrochemical microscopy allowed sequential OCR measurement of the identical EB sample for more than 24 h. We found that the OCR value for the undifferentiated EB (cultured for 2 days) reached almost zero after 12 h exposure in PBS. On the contrary, the OCR value for the differentiated EB (cultured for 8 days) indicated 40 to 28% normal to the OCR at 0 h-incubation after 24 h exposure in PBS-based solution. It is suggested that differentiated cells indicated a stronger adaptation to glucose deprivation comparing to the undifferentiated cells. (C) The Electrochemical Society of Japan, All rights reserved.

DOI： 10.5796/electrochemistry.84.302

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER ADVANCED TECHNOLOGY  

This paper describes potentiometric bioimaging for enzyme activity using a large-scale integration (LSI)-based electrochemical device with 400 sensors. Potentiometric detection is useful for bioimaging because redox species are not consumed or produced during the detection process; therefore, there is no effect on cell activity and the detectable signal is sustained. In this study, the potentiometer mode of the LSI-based device was applied for the detection of glucose oxidase (GOx) and alkaline phosphatase (ALP) activity. The enzyme activities were quantitatively detected within the concentration ranges of 25-250 mu g/mL and 0.10-5.0 ng/mL. In addition, GOx activity in hydrogels and the ALP activity of embryoid bodies (EBs) from embryonic stem (ES) cells were successfully imaged based on detection of the open circuit potentials of individual sensors in real time. To the best of our knowledge, this is the first report of potentiometric imaging using LSI-based electrochemical arrays to detect enzyme activity in ES cells. The LSI-based device is thus demonstrated to be a promising tool for bioimaging of enzyme activity. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.bios.2015.10.021

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：FRONTIERS MEDIA SA  

All living organisms bear its defense mechanism. Immune cells during invasion by foreign body undergoes phagocytosis during which monocyte and neutrophil produces reactive oxygen species (ROS). The ROS generated in animal cells are known to be involved in several diseases and ailments, when generated in excess. Therefore, if the ROS generated in cells can be measured and analyzed precisely, it can be employed in immune function evaluation and disease detection. The aim of the current study is to introduce our newly developed chip-type biosensor device with high specificity and sensitivity. It comprises of counter electrode and working electrodes I and II. The counter electrode is a platinum plate while the working electrodes I and II are platinum microelectrode and osmium-horseradish peroxidase modified gold electrode, respectively which acts as oxygen and hydrogen peroxide (H2O2) detection sensors. Simultaneous measurement of oxygen consumption and H2O2 generation were measured in animal cells under the effect of exogenous addition of differentiation inducer, phorbol 12-myristate 13-acetate. The results obtained showed considerable changes in reduction currents in the absence and

DOI： 10.3389/fphys.2016.00109

Web of Science

PubMed

Language：Japanese  

Language：Japanese  

Language：English   Publishing type：Research paper (international conference proceedings)  

In this study, we constructed a new electrochemical imaging system using a chip device for detecting multiple cell activities simultaneously. This system can acquire multicolor electrochemical images composed of electrochemical signals from two different electrodes which were applied with different potentials. We successfully applied the system to measuring respiration activity and dopamine release from a PC12 cell aggregate at the same time. The new imaging system will be a widely applicable analytical method for cell analysis.

Language：English  

In this study, we constructed a new electrochemical imaging system using a chip device for detecting multiple cell activities simultaneously. This system can acquire multicolor electrochemical images composed of electrochemical signals from two different electrodes which were applied with different potentials. We successfully applied the system to measuring respiration activity and dopamine release from a PC12 cell aggregate at the same time. The new imaging system will be a widely applicable analytical method for cell analysis.

Scopus

Language：Japanese   Publisher：電気関係学会東北支部連合大会実行委員会  

DOI： 10.11528/tsjc.2016.0_38

Language：English   Publishing type：Research paper (scientific journal)   Publisher：PERGAMON-ELSEVIER SCIENCE LTD  

We report on the effect of molecular structure and substrate material on amorphous carbon nitride (a-CN:H) electrode properties including film adhesion to the substrate and electrochemical properties. Films were prepared by neutral beam enhanced chemical vapor deposition on different substrate materials (p-type Si, Cu, Ti, and Pt) below room temperature. When depositing on Si, doping nitrogen into carbon improved the electrochemical properties despite weak adhesion to the substrate. Nitrogen in a-CN:H formed two different bonding configurations:incorporation into aromatic carbon rings and hydrogen nitride by infrared (IR) spectroscopy. Therefore, delocalization of pi bonds by incorporation of nitrogen affected the electrochemical improvement of the a-CN:H electrode. For samples deposited on a different metal substrate, the adhesion to substrate increased as a function of decreasing oxygen concentration on the metal substrate surface; the Pt substrate performed well with no delamination in our evaluation. The electrochemical properties were improved only in the case of deposition on Pt. Moreover, Pt surface modification by hydrogen beam was also effective; consequently, the electroc

DOI： 10.1016/j.carbon.2015.05.074

Web of Science

Language：Japanese   Publisher：Institute of Electrical Engineers of Japan  

Language：English   Publishing type：Research paper (international conference proceedings)  

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC  

This paper reports on the fabrication of a complementary metal oxide semiconductor (CMOS)-based 20 x 20 amperometric sensor array integrated with boron-doped diamond (BDD) microelectrodes. The BDD electrodes were formed on a Si wafer at 800 degrees C, and then transferred to a 0.18 mu m CMOS large-scale integration (LSI) wafer with a benzocyclobutene bonding interlayer. As a result, the BDD microelectrodes were arrayed without significant damage to CMOS circuit or BDD electrodes. The integrated BDD electrodes on the CMOS LSI exhibited excellent performance for electrochemical analysis. The wider potential window and smaller background current compared with Au microelectrodes were experimentally verified. The electron transfer rate to ferrocenemethanol as a standard reagent was large. The fully implemented device successfully detected 100-nm histamine, and was used for the 2-D real-time imaging of histamine diffused in a solution.

DOI： 10.1109/JMEMS.2014.2360837

Web of Science

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (other science council materials etc.)  

J-GLOBAL

Language：English   Publishing type：Research paper (scientific journal)   Publisher：JAPAN SOC ANALYTICAL CHEMISTRY  

In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 p.m. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

DOI： 10.2116/analsci.31.715

Web of Science

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 mu m for rapid electroehemical imaging of large, areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopai-nine release fibril the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bia-LSI clearly showed the effects of the dopaminergic drugs L-3,4-dihydroxyphenylalanine (L-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Out results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.

DOI： 10.1021/acs.analchem.5b01307

Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER CHEMICAL SOC  

In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 mu m for rapid electroehemical imaging of large, areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopai-nine release fibril the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bia-LSI clearly showed the effects of the dopaminergic drugs L-3,4-dihydroxyphenylalanine (L-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Out results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.

DOI： 10.1021/acs.analchem.5b01307

Language：Japanese   Publishing type：Research paper (research society, symposium materials, etc.)  

J-GLOBAL

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER SCIENCE SA  

Feedback mode-based electrochemical imaging of conductivity and topography for large substrate surfaces is presented using a large-scale integration (LSI)-based amperometric chip device with 400 sensors at a pitch of 250 lam. The LSI-based chip device has enabled rapid electrochemical imaging of large substrate surfaces, compared to scanning electrochemical microscope (SECM). Substrates modified with conductive and insulating materials were placed onto the device to acquire electrochemical signals from the substrate surface using positive and negative feedback signals. The conductivity and topography of the substrate were successfully imaged, indicating that the feedback mode-based electrochemical imaging with such a device is useful to characterize large-area substrate surfaces. (C) 2015 Elsevier B.V. All rights reserved.

DOI： 10.1016/j.jelechem.2015.01.020

Web of Science

Language：Japanese  

Language：English   Publishing type：Research paper (international conference proceedings)  

© 15CBMS-0001. In this study, we demonstrated a potentiometric imaging of enzyme activity in mouse embryonic stem (ES) cells using an LSI-based electrochemical chip device with 400 microelectrodes. Successful results on the detection of the alkaline phosphatase activity of ES cells show that the potentiometric imaging using our LSI-based device will be a widely applicable method for cell analysis.

Language：English  

© 15CBMS-0001. In this study, we demonstrated a potentiometric imaging of enzyme activity in mouse embryonic stem (ES) cells using an LSI-based electrochemical chip device with 400 microelectrodes. Successful results on the detection of the alkaline phosphatase activity of ES cells show that the potentiometric imaging using our LSI-based device will be a widely applicable method for cell analysis.

Scopus

Language：English  

We report a large-scale integration (LSI) based amperometric chip device containing 400 sensors with a pitch of 250 μm and its applications for biological analysis of dopamine release from three-dimensional (3D) cultured cells using rat pheochromocytoma (PC12) cells as a neural cell model. The 3D cultured PC12 cells (PC12 spheroids) were stimulated with a high K+ solution to induce dopamine release. We showed clearly drug effects of dopaminergic drugs L-3,4-dihydroxyphenylalanine, (L-DOPA) and reserpine on dopamine release from PC12 spheroids. Our results indicate that the device is a useful tool for evaluating the effects of dopaminergic drugs.

Scopus

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROYAL SOC CHEMISTRY  

We have developed a large-scale integrated (LSI) complementary metal-oxide semiconductor (CMOS)-based amperometric sensor array system called "Bio-LSI" as a platform for electrochemical bio-imaging and multi-point biosensing with 400 measurement points. In this study, we newly developed a Bio-LSI chip with a light-shield structure and a mode-selectable function with the aim of extending the application range of Bio-LSI. The light shield created by the top metal layer of the LSI chip significantly reduces the noise generated by the photocurrent, whose value is less than 1% of the previous Bio-LSI without the light shield. The mode-selectable function enables the individual operation of 400 electrodes in off, electrometer, V1, and V2 mode. The off-mode cuts the electrode from the electric circuit. The electrometer-mode reads out the electrode potential. The V1-mode and the V2-mode set the selected sensor electrode at two different independent voltages and read out the current. We demonstrated the usefulness of the mode-selectable function. First, we displayed a dot picture based on the redox reactions of 2.0 mM ferrocenemethanol at 400 electrodes by applying two different independent

DOI： 10.1039/c4lc01099j

Web of Science

PubMed

Language：Japanese  

Authorship：Lead author   Language：English  

Language：English  

Language：English  

Language：Japanese   Publishing type：(MISC) Introduction and explanation (commerce magazine)   Publisher：株式会社オプトロニクス社  

Language：Japanese  

Language：English  

Language：Japanese  

J-GLOBAL

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (others)   Publisher：The Electrochemical Society of Japan  

DOI： 10.5796/electrochemistry.82.202

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (others)   Publisher：The Electrochemical Society of Japan  

DOI： 10.5796/electrochemistry.82.202

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ROYAL SOC CHEMISTRY  

We have previously reported a local redox cycling-based electrochemical (LRC-EC) system for the incorporation of many electrochemical sensors into a small chip device. In the present study, a new type of LRC-EC chip device was fabricated for the detection of a droplet array. To detect electrochemically redox compounds in droplets, Pt pseudo-reference/counter electrodes were incorporated into the individual sensors of the LRC-EC chip device. Cyclic voltammetry for the LRC-EC chip device with internal Pt pseudo-reference electrodes indicated well-defined voltammograms based on redox cycling for the individual sensor points. The device was successfully applied to the addressable detection of alkaline phosphatase (ALP) activity of HeLa cells in single droplets on the sensor points. Therefore, the LRC-EC chip device is considered to be a useful device for the bioanalysis of droplet systems.

DOI： 10.1039/c3lc51156a

Web of Science

PubMed

Language：Japanese   Publisher：Institute of Electrical Engineers of Japan  

Language：Japanese  

Language：Japanese  

Language：Japanese   Publishing type：(MISC) Introduction and explanation (others)  

Language：Japanese   Publishing type：(MISC) Introduction and explanation (others)  

CiNii Books

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese  

Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：株式会社ティー・アイ・シィー  

CiNii Books

Language：Japanese   Presentation type：Oral presentation(general)  

Event date： 2023.3

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

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Event date： 2023.2

Language：Japanese   Presentation type：Poster presentation  

Venue：⼤阪⼤学産業科学研究所  

Event date： 2023.1

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Event date： 2022.12

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Event date： 2022.11

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Event date： 2022.11

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Venue：京都大学 桂キャンパス 桂ホール（Bクラスター 事務管理棟1階）  

Event date： 2022.11

Language：Japanese   Presentation type：Poster presentation  

Venue：京都大学 桂キャンパス 桂ホール（Bクラスター 事務管理棟1階）  

Event date： 2022.10

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Event date： 2022.9

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Event date： 2022.9

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Event date： 2022.9

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Event date： 2022.9

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Venue：徳島⼤学ポストLEDフォトニクス研究所  

Event date： 2022.9

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Event date： 2022.6

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Venue：東京工業大学　ディジタル多目的ホール（大岡山キャンパス西9号館）  

Event date： 2022.5

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Event date： 2022.3

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Event date： 2022.3

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Event date： 2022.1

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Event date： 2021.12

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：パシフィコ横浜ノース G214  

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Event date： 2021.12

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Event date： 2020.12

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Venue：WEB開催  

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Event date： 2020.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：WEB開催  

Language：Japanese   Presentation type：Oral presentation(general)  

Event date： 2020.10

Language：English   Presentation type：Oral presentation(invited, special)  

Venue：Digital Meeting  

Event date： 2020.10 - 2020.11

Language：English   Presentation type：Oral presentation(invited, special)  

Event date： 2020.9

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：WEB開催・一部浜松町コンベンションホール開催  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Event date： 2020.7

Language：Japanese   Presentation type：Poster presentation  

Language：Japanese  

Event date： 2019.11

Language：English   Presentation type：Oral presentation(invited, special)  

Venue：Grand Inna Sanur Bali, Indonesia  

Language：English   Presentation type：Oral presentation(invited, special)  

Event date： 2019.11

Language：English   Presentation type：Poster presentation  

Venue：National Taiwan University, Taipei, Taiwan  

Language：English   Presentation type：Poster presentation  

Language：Japanese   Presentation type：Oral presentation(general)  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：壱岐の島ホール  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：金沢大学 角間キャンパス  

Language：English   Presentation type：Oral presentation(general)  

Venue：Nuovo Polo Congressuale Bologna, Italy  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：English   Presentation type：Oral presentation(general)  

Venue：University of Vienna, Austria  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：松島新富亭  

Language：Japanese   Presentation type：Other  

Language：English   Presentation type：Oral presentation(invited, special)  

Venue：ohoku University, Japan  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京理科大学葛飾キャンパス  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：海峡メッセ下関  

Language：English   Presentation type：Oral presentation(general)  

Venue：Pan Pacific Hanoi, Hanoi, Vietnam  

Language：English   Presentation type：Oral presentation(invited, special)  

Venue：Xi’an Jiaotong University, Xi’an China  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：東京理科大学葛飾キャンパス  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：English   Presentation type：Oral presentation(general)  

Venue：La Doua Campus of Claude Bernard University Lyon 1  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：首都大学東京南大沢キャンパス，東京  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：沖縄県宮古島市中央公民館大ホール，沖縄  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：北海道大学 札幌  

Language：English   Presentation type：Oral presentation(general)  

Venue：Ramada Plaza Jeju, Jeju Island, Korea  

Language：Japanese   Presentation type：Oral presentation(general)  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：English   Presentation type：Oral presentation(general)  

Venue：Penang, Malaysia  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：深谷  

Language：English   Presentation type：Oral presentation(general)  

Venue：Sweden Malmö  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：横浜  

Language：English   Presentation type：Other  

Venue：英国大使館，東京  

Language：English   Presentation type：Symposium workshop panel(nominated)  

Venue：Tokyo  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：京都  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：仙台  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：札幌  

Language：English   Presentation type：Oral presentation(general)  

Venue：Taiwan Hsinchu  

Language：English   Presentation type：Poster presentation  

Venue：Switzerland Lausanne  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：郡山  

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：吹田  

電気化学会女性躍進賞受賞講演

Language：English   Presentation type：Oral presentation(general)  

Venue：Thailand Chiang Mai  

Language：English   Presentation type：Oral presentation(general)  

Venue：United States San Francisco  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：大阪  

Language：English   Presentation type：Oral presentation(general)  

Venue：JAPAN Tokyo  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：金沢  

Language：English   Presentation type：Oral presentation(general)  

Venue：Czech Republic Prague  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：浜松  

Language：English   Presentation type：Oral presentation(general)  

Venue：Taiwan Chientan Youth Activity Center, Taipei  

ACCS Best Paper Presentation award受賞

Language：English   Presentation type：Oral presentation(general)  

Venue：JAPAN 新潟  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：横浜国立大学  

震災のため大会中止．要旨集での発表のみ．

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：ビッグサイト，東京  

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：東北大学，仙台  

Language：English   Presentation type：Oral presentation(general)  

Venue：France Nice Acropolis  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：富山大 五福  

Language：English   Presentation type：Oral presentation(general)  

Venue：JAPAN 東北大学川内萩ホール  

Language：English   Presentation type：Oral presentation(general)  

Venue：Republic of Korea Exco, Daegu  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：京大 吉田  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東工大 すずかけ台  

Language：English   Presentation type：Oral presentation(general)  

Venue：Taiwan National Tsing Hua University  

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東工大 大岡山  

Application no：特願2018-157868  Date applied：2018.8

Announcement no：特開2020-030192  Date announced：2020.2

Patent/Registration no：6757079  Date registered：2020.9 

Country of applicant：Domestic  

Applicant：国立大学法人東北大学

Application no：特願2018-157868  Date applied：2018.8

Announcement no：特開2020-030192  Date announced：2020.2

Patent/Registration no：特許6757079  Date registered：2020.9 

J-GLOBAL

Applicant：国立大学法人東北大学

Application no：特願2018-157868  Date applied：2018.8

Announcement no：特開2020-030192  Date announced：2020.2

Patent/Registration no：特許第6757079号  Date registered：2020.9 

J-GLOBAL

Applicant：株式会社 ハプロファーマ, 国立大学法人東北大学

Application no：JP2018016262  Date applied：2018.4

Publication no：WO2018-194152  Date published：2018.10

J-GLOBAL

Applicant：株式会社 ハプロファーマ, 国立大学法人東北大学

Application no：JP2018016261  Date applied：2018.4

Publication no：WO2018-194151  Date published：2018.10

J-GLOBAL

Application no：特願2017-197217  Date applied：2017.10

Announcement no：特開 2018-72331  Date announced：2018.5

Application no：特願2017-084812  Date applied：2017.4

Publication no：再表2018/194151  Date published：2018.10

Country of applicant：Foreign country  

Application no：特願2017-084764  Date applied：2017.4

Publication no：WO2018-194152  Date published：2018.10

J-GLOBAL

Application no：特願2017-084812  Date applied：2017.4

Publication no：WO2018-194151  Date published：2018.10

J-GLOBAL

Application no：特願2018-500152  Date applied：2017.2

Publication no：再表2017/141961  Date published：2017.8

Country of applicant：Foreign country  

Application no：特願2016-208519  Date applied：2016.10

Application no：特願2016-197818  Date applied：2016.10

Announcement no：特開2018-059823  Date announced：2018.4

Patent/Registration no：特許第6218199号  Date registered：2017.10 

Application no：特願2016-197818  Date applied：2016.10

Application no：特願2016-101043  Date applied：2016.5

Announcement no：特開2017-207410  Date announced：2017.11

Patent/Registration no：特許第6344775号  Date registered：2018.6 

Country of applicant：Foreign country  

Application no：特願2016-101043  Date applied：2016.5

Application no：特願2016-087924  Date applied：2016.4

Announcement no：特開2017-198493  Date announced：2017.11

Patent/Registration no：特許第61160806号  Date registered：2017.3 

Country of applicant：Domestic  

Application no：特願2016-087924  Date applied：2016.4

Application no：特願2016-056567  Date applied：2016.3

Announcement no：特開2017-173014  Date announced：2017.9

Application no：特願2016-056567  Date applied：2016.3

Application no：特願2016-055251  Date applied：2016.3

Announcement no：特開2017-167104  Date announced：2017.9

Patent/Registration no：特許第6579325号  Date registered：2019.9 

Applicant：日本航空電子工業株式会社, 国立大学法人東北大学

Application no：特願2016-055283  Date applied：2016.3

Announcement no：特開2017-116517  Date announced：2017.6

J-GLOBAL

Application no：特願2016-055251  Date applied：2016.3

Announcement no：特開2017-167104  Date announced：2017.9

Patent/Registration no：特許第6579325号  Date registered：2019.9 

J-GLOBAL

Application no：特願2016-018358  Date applied：2016.2

Announcement no：特開2017-138164  Date announced：2017.8

Application no：特願2016-018358  Date applied：2016.2

Announcement no：特開2017-138164  Date announced：2017.8

J-GLOBAL

Application no：特願2015-249764  Date applied：2015.12

Announcement no：特開2017-116517  Date announced：2017.6

Application no：特願2015-228042  Date applied：2015.11

Patent/Registration no：特許6116075  Date issued：2017.3

Application no：特願2015-159962  Date applied：2015.8

Announcement no：特開2017-37044  Date announced：2017.2

Patent/Registration no：特許第6562765号  Date registered：2019.8 

Country of applicant：Domestic  

Applicant：大日本印刷株式会社, 国立大学法人東北大学

Application no：特願2015-159962  Date applied：2015.8

Announcement no：特開2017-037044  Date announced：2017.2

Patent/Registration no：特許第6557090号  Date registered：2019.7 

J-GLOBAL

Application no：特願2015-159963  Date applied：2015.8

Announcement no：特開2017-37045  Date announced：2017.2

Patent/Registration no：特許第6514991号  Date registered：2019.4 

Applicant：大日本印刷株式会社, 国立大学法人東北大学

Application no：特願2015-159960  Date applied：2015.8

Announcement no：特開2017-037043  Date announced：2017.2

Patent/Registration no：特許第6562765号  Date registered：2019.8 

J-GLOBAL

Application no：特願2015-159963  Date applied：2015.8

Announcement no：特開2017-37045  Date announced：2017.2

Application no：特願2015-159962  Date applied：2015.8

Announcement no：特開2017-37044  Date announced：2017.2

Patent/Registration no：特許第6562765号  Date registered：2019.8 

J-GLOBAL

Application no：特願2015-47649  Date applied：2015.3

Announcement no：特開2015-187607  Date announced：2015.10

Patent/Registration no：特許第5916176号  Date registered：2016.4 

Country of applicant：Domestic  

Application no：特願2014-070156  Date applied：2014.3

Announcement no：特開2015-190939  Date announced：2015.11

Patent/Registration no：特許第6240543号  Date registered：2017.11 

Country of applicant：Domestic  

Application no：特願2014-070156  Date applied：2014.3

Announcement no：特開2015-190939  Date announced：2015.11

J-GLOBAL

Application no：特願2014-047888  Date applied：2014.3

Announcement no：特開2015-187607  Date announced：2015.10

Applicant：国立大学法人東北大学，日本航空電子工業株式会社，凸版印刷株式会社

Application no：特願2011-234459  Date applied：2011.10

Announcement no：特開2013-092437  Date announced：2013.5

Patent/Registration no：特許第5769020号  Date registered：2015.8 

Country of applicant：Domestic  

Applicant：国立大学法人東北大学，日本航空電子工業株式会社，凸版印刷株式会社

Application no：2011-234459  Date applied：2011.10

Announcement no：2013-092437  Date announced：2013.5

Applicant：日本航空電子工業株式会社，国立大学法人東北大学

Application no：特願2010-292608  Date applied：2010.12

Announcement no：特開2012-141173  Date announced：2012.7

Patent/Registration no：特許第4933657号  Date registered：2012.2 

Applicant：日本航空電子工業株式会社，国立大学法人東北大学

Application no：特願2010-292608  Date applied：2010.12

Announcement no：特開2012-141173  Date announced：2012.7

Patent/Registration no：特許4933657  Date issued：2012.2

Applicant：国立大学法人東北大学

Application no：特願2010-277329  Date applied：2010.12

Announcement no：特開2012-127695  Date announced：2012.7

J-GLOBAL

Applicant：国立大学法人東北大学

Application no：2010-277329  Date applied：2010.12

Announcement no：2012-127695  Date announced：2012.7

Applicant：ニプロ株式会社，国立大学法人東北大学

Application no：特願2006-186109  Date applied：2006.7

Announcement no：特開2008-14791  Date announced：2008.1

Applicant：ニプロ株式会社，国立大学法人東北大学

Application no：特願2006-186109  Date applied：2006.7

Announcement no：特開2008-14791  Date announced：2008.1

Applicant：株式会社島津製作所

Application no：特願平8-167954  Date applied：1996.6

Announcement no：特開平10-019839  Date announced：1998.1

Patent/Registration no：特許第3684682号  Date registered：2005.6 

J-GLOBAL

Applicant：株式会社島津製作所

Application no：特願平8-167954  Date applied：1996.6

Announcement no：特開平10-19839  Date announced：1998.1

Award type：International academic award (Japan or overseas)  Country：Japan

Award type：International academic award (Japan or overseas)  Country：Japan

Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

Award type：Award from international society, conference, symposium, etc.