Updated on 2024/08/30

写真a

 
Kumi Inoue
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Engineering Basic Sciences ( Basic Education Center) Associate Professor
Title
Associate Professor
Contact information
メールアドレス

Research History

  • University of Yamanashi   Center for Basic Education, Faculty of Engineering, Graduate Faculty of Interdisciplinary Research   Associate Professor

    2020.8

      More details

    Country:Japan

    Job classification:Associate professor/Lecturer (full time)

  • Tohoku University   Graduate School of Environmental Studies   Part-time faculty   Visiting Associate Professor

    2022.4

      More details

    Country:Japan

    Job classification:Lecturer (part-time)

  • センスチップ株式会社   取締役

    2020.2

  • Tohoku University   Graduate School of Environmental Studies   Associate Professor

    2019.4 - 2022.3

  • Tohoku University   Graduate School of Environmental Studies   Associate Professor

    2018.4 - 2019.3

  • COI Tohoku   Assistant Research Leader

    2014.4 - 2022.3

  • Tohoku University   Graduate School of Environmental Studies   Senior Assistant Professor

    2014.4 - 2018.3

  • Tohoku University   Micro System Integration Center   Assistant Professor

    2013.4 - 2014.3

  • Tohoku Institute of Technology   Part-time Lecturer

    2011.4 - 2016.9

  • Tohoku University   Micro System Integration Center   Research Fellow

    2010.4 - 2013.3

  • Tohoku University   Graduate School of Environmental Studies   Experimental Assistant

    2005.2 - 2010.3

  • Miyagi Prefecture Miso and Soy Sauce Industrial Cooperative   Engineering Department   Senior Researcher

    2002.4 - 2005.2

  • Miyagi Prefecture Miso and Soy Sauce Industrial Cooperative   Engineering Department   Researcher

    1995.11 - 2002.3

▼display all

Education

  • Tohoku University   Graduate School, Division of Environmental Science

    2007.10 - 2010.8

      More details

    Country: Japan

  • Kyoto University   Faculty of Agriculture   農芸化学科

    1991.4 - 1995.3

      More details

    Country: Japan

Degree

  • 博士(学術) ( 東北大学 )

Research Areas

  • Nanotechnology/Materials / Bio chemistry

  • Nanotechnology/Materials / Analytical chemistry  / Electro Analytical Chemistry

Research Interests

  • Bioimaging

  • Electroanalytical Chemistry

  • バイオセンサ

Research Projects

  • 亜硫酸オキシダーゼを用いるワイン中亜硫酸の簡易定量法の開発

    2023.12 - 2024.2

    山梨大学  令和5年度 ダイバーシティ研究環境実現イニシアティブ(牽引型)   女性研究者対象 地域貢献型

    井上久美、斉藤史恵、久保田恒喜

      More details

    Authorship:Principal investigator  Type of fund::Others

  • ワイン製造管理のための簡便な分子状亜硫酸モニタリングシステムの開発

    Grant number:JPMJSF23CS  2023.10 - 2025.3

    JST  大学発新産業創出基金事業 可能性検証  大学発新産業創出基金事業 可能性検証

      More details

    Authorship:Principal investigator  Grant type:Competitive  Type of fund::Funded research

  • プロテアーゼ反応を用いる高感度・小型・簡便な電気化学センサ

    2023.4 - 2024.3

    一般財団法人天野エンザイム科学技術振興財団  第24回 酵素応用シンポジウム研究奨励賞 

      More details

    Authorship:Principal investigator  Type of fund::Donation

  • 病気の原因を「取り出す・見る」技術の開発

    2023.4 - 2024.3

    山梨大学  令和5年度 学術・社会変革研究プロジェクト 

    井上 久美、研究分担者 ・浮田 芳昭 (工学部機械工学科、准教授) ・吉野 修 (医学部 産婦人科、准教授) ・井原 達矢 (医学部 泌尿器科、講師) ・篠崎 陽一 (医学部 薬理学、准教授) 研究協力者 ・尾山 恵亮 (医学部 産婦人科、臨床助教) ・阿部 岳晃(工学域 博士課程2年) ・松田 結(医学部 泌尿器科、大学院生) ・久保田 友人(医学部 薬理学、大学院生) ・久保田 恒喜(工学部基礎教育センター、研究員・応用化学科博士課程3年)

      More details

    Authorship:Principal investigator  Type of fund::Others

  • バイポーラ電気化学発光顕微鏡による細胞間物質伝達の可視化

    2023.1 - 2023.12

    公益財団法人 光科学技術研究振興財団  令和3年度 研究助成 

    井上 久美

      More details

    Authorship:Principal investigator  Grant type:Competitive  Type of fund::Funded research

  • バイオをはかる、バイオではかる

    2022.11 - 2023.3

    山梨大学男女共同参画推進室  山梨大学男女共同参画学術研究奨励賞 

      More details

    Authorship:Principal investigator  Type of fund::Others

  • n vivo imaging of intercellular transmitters using thermal drawing bipolar electrode array probe

    Grant number:22K18941  2022.6 - 2025.3

    Japan Society for the Promotion of Science  University of Yamanashi  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

  • 生体膜機能を可視化する電気化学発光顕微鏡の創製

    2022.5 - 2023.3

    国立大学法人東北大学 電気通信研究所長  令和4年度電気通信研究所共同プロジェクト研究 

    平本 薫

      More details

    Authorship:Coinvestigator(s)  Type of fund::Others

  • バイポーラ電気化学顕微鏡による生命システムの計測

    2022.4 - 2029.3

    国立研究開発法人 科学技術振興機構  創発的研究支援事業 

    井上 久美

      More details

    Authorship:Principal investigator  Type of fund::Funded research

    細胞間の物質伝達を直接観察できる、これまでにない解像度(250 nm)と高速性(75 fps)を併せ持つ電気化学顕微鏡を創出します。バイポーラ電極素子を工夫することで、自由行動ラットの脳内情報やエネルギー物質伝達の可視化に挑戦します。本技術は、バイオイメージング法の新しい学術領域を創成するとともに、生命システム解明への道を開き、生命を模した超省エネルギー化など、イノベーション創出の基盤となります。

  • 高分解能・高速バイポーラ電気化学顕微鏡による細胞間コミュニケーションの可視化

    Grant number:22H02105  2022.4 - 2025.3

    日本学術振興会  科学研究費助成事業 基盤研究(B)  基盤研究(B)

    井上 久美, 鈴木 孝明

  • バイポーラ電気化学発光顕微鏡による細胞間物質伝達の可視化

    2022.1 - 2022.12

    公益財団法人 光科学技術研究振興財団  令和3年度 研究助成 

      More details

    Authorship:Principal investigator  Type of fund::Donation

  • バイポーラ電気化学顕微鏡による生命システムの計測

    2022 - 2028

    科学技術振興機構  戦略的な研究開発の推進 創発的研究支援事業 

    井上 久美

      More details

    Authorship:Principal investigator 

    細胞間の物質伝達を直接観察できる、これまでにない解像度(250 nm)と高速性(75 fps)を併せ持つ電気化学顕微鏡を創出します。バイポーラ電極素子を工夫することで、自由行動ラットの脳内情報やエネルギー物質伝達の可視化に挑戦します。本技術は、バイオイメージング法の新しい学術領域を創成するとともに、生命システム解明への道を開き、生命を模した超省エネルギー化など、イノベーション創出の基盤となります。

  • 細胞間物質伝達を光で可視化するバイポーラ電気化学顕微鏡素子の高感度化に関する研究

    2021.12 - 2022.2

    令和3年度ダイバーシティ研究環境実現イニシアティブ(牽引型) 女性研究者対象・地域貢献型研究支援 

    井上 久美、久保田恒喜

      More details

    Authorship:Principal investigator  Type of fund::Others

  • Development of ultra-high resolution electrochemical bioimaging method to elucidate intercellular communication

    Grant number:19K05687  2019.4 - 2022.3

    Japan Society for the Promotion of Science  Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    INOUE Kumi Y

      More details

    Grant type:Competitive 

    To elucidate cell-cell communication, a chemical microscopy is required to quantitatively observe chemical information that changes dynamically in a local region near the cell. An electrochemical imaging technique using microelectrode is expected to observe the activity of cells with minimal invasion. However, conventional electrochemical microscopy has a trade-off between temporal resolution and spatial resolution. In this study, we realized high-resolution and high-speed electrochemical imaging by using a micro bipolar electrode array that does not require a lead wire to each electrode. We also demonstrated biological activity imaging including cell respiration imaging and dopamine imaging using this platform.

  • 抗原修飾ヤヌス粒子による簡易計測装置

    2016.8 - 2020.3

    Japan Agency for Medical Research and Development (AMED)  Medical Research and Development Programs Focused on Technology Transfers: Development of Advanced Measurement and Analysis Systems 

    Hiroshi Yabu

      More details

    Grant type:Competitive 

  • Hybrid electrochemical bioimaging with multiscale

    Grant number:16H02280  2016.4 - 2019.3

    Japan Society for the Promotion of Science (JSPS)  Tohoku University  Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Tomokazu Matsue

      More details

    Grant type:Competitive 

    It is important to visualize cell functions for evaluation of tissues and organs in regenerative medicine, and tissue engineering. As a low-invasive method, electrochemical imaging has been proposed using several electrochemical devices such as electrode arrays. The method can convert cell functions, such as chemical secretion and consumption, enzyme activity, and respiratory activity, to electrochemical signals for evaluating them. In conventional electrochemical imaging using electrode arrays, only single targets are electrochemically monitored in real time. In addition, the spatial resolution is low. To improve this detection system, we have proposed multi-scaled electrochemical imaging system showing multiple scales of electrochemical signal and spatial resolution.

  • Center of Innovation for creation of a health-conscious society to realize healthy and fulfilling life, and strengthen family ties through unobtrusive sensing and daily health screening

    2013.11 - 2022.3

    Japan Science and Technology Agency  The Center of Innovation Program 

    Iwao Waga

      More details

    Grant type:Competitive 

  • Innovative electrochemical imaging device for bioanalysis

    Grant number:25248032  2013.5 - 2016.3

    Japan Society for the Promotion of Science (JSPS)  Tohoku University  Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Tomokazu Matsue

      More details

    Grant type:Competitive 

    In the present study, we have developed two-types of electrochemical devices for bioimaging. One was a bio-LSI device which was based on large-scale integrated (LSI) circuits dedicated to bioanalysis. The other was a local redox cycling-based electrochemical (LRC-EC) device utilizing local redox cycling. The device consisted of many switching sensors incorporated into a small chip device to detect electrochemical responses. These bioimaging devices were successfully applied for evaluation of oxygen consumption and secreted chemicals from cells, and cell differentiation.

  • Development of the novel principles of electrochemical sensors using zymogens as sensing elements

    Grant number:23750073  2011.4 - 2013.3

    Japan Society for the Promotion of Science (JSPS)  Tohoku University  Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    INOUE Kumi Y

      More details

    Grant type:Competitive 

    We have developed novel type electrochemical sensors using zymogens as sensor elements. First, we made electrochemical endotoxin sensors based on the Limulus amebocyte lysate reagent containing zymogens. We successfully developed a cost effective screen-printed sensor chip with practical sensitivity. We also developed a highly sensitive device with a practical structure for stripping voltammetry. Then, we extended this zymogen-based sensing principle into other assays. We successfully detected the apoptosis of HepG2 cells using electrochemical methods. These results contribute not only the development of the sensor engineering but also the clinical safety and biological studies.

▼display all

Papers

  • 学会レポート 第14回アジア化学センサ会議(ACCS 2023)

    井上久美, 上田太郎, 田村真治

    Chemical Sensors   40 ( 1 )   2024.3

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (others)  

  • Correlation Between Electrochemiluminescence and Fluorescence Properties of Fluorescent Dye Compounds

    Koki Kubota, Ayane Endo, Kumi Y. Inoue

    Chemical Sensors   40 ( Suppl. A )   7 - 9   2024.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • Enhancement of Electrochemiluminescence by Au Paste Electrode for Bipolar Electroanalysis Reviewed

    Ayane ENDO, Koki KUBOTA, Takahiro ITO-SASAKI, Mayo KOMATSU, Tomoki IWAMA, Hitoshi SHIKU, Kumi Y. INOUE

    Electrochemistry   92 ( 2 )   022013 - 022013   2023.12( ISSN:1344-3542  eISSN:2186-2451 )

     More details

    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Electrochemical Society of Japan  

    DOI: 10.5796/electrochemistry.23-68114

  • Relationship between Plasma Lipopolysaccharide Concentration and Health Status in Healthy Subjects and Patients with Abnormal Glucose Metabolism in Japan: A Preliminary Cross-Sectional Study Reviewed

    Nobuo Fuke, Shojiro Sawada, Takahiro Ito-Sasaki, Kumi Y. Inoue, Yusuke Ushida, Ikuo Sato, Tomokazu Matsue, Hideki Katagiri, Hiroyuki Ueda, Hiroyuki Suganuma

    J. Multidiscip. Res.   6 ( 4 )   605 - 626   2023.11(  eISSN:2571-8800 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    Lipopolysaccharides are components of Gram-negative bacteria. The relationship between blood lipopolysaccharide levels and health status has mainly been investigated in Europe, and there is a lack of information about Asia, particularly Japan. This study aimed to investigate the relationship between blood lipopolysaccharide levels and health status in the Japanese. We conducted two cross-sectional studies in 36 healthy subjects (Study 1) and 36 patients with abnormal glucose metabolism (AGM; Study 2). The plasma lipopolysaccharide concentration in healthy subjects was positively correlated with body mass index. The plasma lipopolysaccharide concentration in AGM patients was obviously higher than that in healthy subjects. Furthermore, in AGM patients, the plasma lipopolysaccharide concentration was positively correlated with C-peptide, fasting plasma glucose levels, triglycerides, and stage of diabetic nephropathy. The plasma lipopolysaccharide concentration was also negatively correlated with 20/(C-peptide × fasting plasma glucose), an indicator of insulin resistance, and high-density lipoprotein cholesterol. In particular, the correlation between plasma lipopolysaccharide concentration and triglycerides in AGM patients was maintained in multiple regression analyses adjusted for age, sex, or body mass index. These results suggest a possible role of lipopolysaccharides in obesity in healthy subjects and in the deterioration of triglyceride metabolism in AGM patients in the Japanese population.

    DOI: 10.3390/j6040040

  • Development of the bipolar electrochemical microscopy for imaging intercellular mediators

    Kumi Y. Inoue

    55 ( 13 )   61 - 64   2023.11

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)  

  • Bipolar Electrochemical Sensor with Perylene Diimide-Based Cathodic Luminophore for Dopamine Detection and Imaging

    Tomoki Iwama, Mayo Komatsu, Kumi Y. Inoue, Koki Kubota, Takahiro Ito-Sasaki, Hitoshi Shiku

    SSRN   2023.11

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.2139/ssrn.4632693

  • C-reactive Protein Detection Using an Ion-sensitive Field-effect Transistor (ISFET)-based Aptasensor with a Chemically Modified Gate Surface for Improved Sensitivity

    Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Masayoshi Esashi, Hitoshi Shiku

    Sensors and Materials   35 ( 10 )   4797 - 4797   2023.10( ISSN:0914-4935  eISSN:2435-0869 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:MYU K.K.  

    DOI: 10.18494/sam4570

  • Effect of Valinomycin on the Respiration Activity of Zebrafish Embryos Using a Large-Scale-Integration-based Multiple Amperometric Biosensor Reviewed

    Kazuki Terao, Masato Suzuki, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Tomoyuki Yasukawa

    Sensors and Materials   35 ( 10 )   4781 - 4788   2023.10( ISSN:0914-4935  eISSN:2435-0869 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MYU K.K.  

    DOI: 10.18494/sam4493

  • Filling method of conductive paste into porous membrane for closed bipolar electrochemical microscope

    Kumi Y. Inoue, Ayane Endo, Koki Kubota, Shion Mizukura, Takahiro Ito-Sasaki, Yuri Totsuka

    Chemical Sensors   39 suppl. B   88 - 90   2023.9

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

    CiNii Research

  • 研究者の最新動向 細胞間伝達物質のイメージングに向けた電気化学顕微鏡の開発 Invited

    井上 久美[安田]

    Precision Medicine   6 ( 8 )   654 - 658   2023.7( ISSN:2434-3625 )

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:(株)北隆館  

    電気化学顕微鏡は細胞近傍の対象分子濃度分布を2次元イメージとして得られる技術である。微小電極を用いて酸化/還元に基づく電流値から濃度情報を得て,電極の位置情報から濃度分布のマッピングを行う。電気化学顕微鏡には大きく分けて2つの方式がある。ひとつは,走査型電気化学顕微鏡であり,1細胞レベルの高解像度イメージングが可能である。もう1つは微小電極アレイであり,秒以下の時間分解能でリアルタイミングイメージングが可能である。我々は,高解像度・高速を両立する方法として,バイポーラ電気化学顕微鏡の研究を行っている。本稿ではこれらの3つの手法について概説する。(著者抄録)

  • Electrochemical bioimaging using bipolar electrodes Invited

    Kumi Y. Inoue

    Chemical Sensors   2023.3

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)  

  • Analysis of electrochemiluminescence behavior by potential difference measurement between closed bipolar electrode

    Kumi Y. Inoue, Takaaki Abe, Koki Kubota, Ayane Endo, Yuri Totsuka.

    Chemical Sensors   39 ( Suppl. A )   2023.3

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (research society, symposium materials, etc.)  

  • Energy-harvesting and mass sensor performances of magnetostrictive cobalt ferrite-spattered Fe–Co alloy plate Reviewed

    Hiroki Kurita, Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Takeru Nakaki, Shotaro Kuroda, Zhenjin Wang, Wakako Araki, Hitoshi Shiku, Fumio Narita

    Journal of Alloys and Compounds   951   169844 - 169844   2023.3( ISSN:0925-8388 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.jallcom.2023.169844

  • Analysis of electrochemiluminescence behavior by potential difference measurement between closed bipolar electrode

    Kumi Y. Inoue, Takaaki Abe, Koki Kubota, Ayane Endo, Yuri Totsuka

    Chemical Sensors   39 ( Suppl. A )   103 - 105   2023.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • Droplet-free digital immunoassay based on electrochemiluminescence Reviewed

    Kentaro Ito, Kumi Y. Inoue*, Kosuke Ino, Hitoshi Shiku*

    Biosensors and Bioelectronics: X   13   100312 - 100312   2023.2( ISSN:2590-1370 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.biosx.2023.100312

  • Highly sensitive electrochemical endotoxin sensor based on redox cycling using an interdigitated array electrode device Invited Reviewed

    Kentaro Ito, Kumi Y. Inoue*, Takahiro Ito-Sasaki, Miho Ikegawa, Shinichiro Takano, Kosuke Ino, Hitoshi Shiku*.

    Micromachines   14 ( 2 )   327   2023.1

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    The Limulus amebocyte lysate (LAL) reaction-based assay, the most commonly used endotoxin detection method, requires a skilled technician. In this study, to develop an easy-to-use and highly sensitive endotoxin sensor, we created an electrochemical endotoxin sensor by using an interdigitated array electrode (IDAE) device with advantages of amplifiable signals via redox cycling and portability. We added Boc-Leu-Gly-Arg-p-aminophenol (LGR-pAP) as an electrochemical substrate for an LAL reaction and detected p-aminophenol (pAP) released from LGR-pAP as a product of an endotoxin-induced LAL reaction via an IDAE device. The IDAE device showed a great redox cycling efficiency of 79.8%, and a 4.79-fold signal amplification rate. Then, we confirmed that pAP was detectable in the presence of LGR-pAP through chronoamperometry with the potential of the anode stepped from -0.3 to 0.5 V vs. Ag/AgCl while the cathode was biased at -0.3 V vs. Ag/AgCl. Then, we performed an endotoxin assay by using the IDAE device. Our endotoxin sensor detected as low as 0.7 and 1.0 endotoxin unit/L after the LAL reaction for 1 h and 45 min, respectively, and these data were within the cut-off value for ultrapure dialysis fluid. Therefore, our highly sensitive endotoxin sensor is useful for ensuring medical safety.

    DOI: 10.3390/mi14020327

    PubMed

  • Batteryless wireless magnetostrictive Fe30Co70/Ni clad plate for human coronavirus 229E detection

    Daiki Neyama, Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Hiroki Kurita, Shion Osana, Naoto Miyamoto, Tsuyoki Tayama, Daiki Chiba, Masahito Watanabe, Hitoshi Shiku, Fumio Narita

    Sensors and Actuators A: Physical   349   114052 - 114052   2023.1( ISSN:0924-4247 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been garnered increasing for its rapid worldwide spread. Each country had implemented city-wide lockdowns and immigration regulations to prevent the spread of the infection, resulting in severe economic consequences. Materials and technologies that monitor environmental conditions and wirelessly communicate such information to people are thus gaining considerable attention as a countermeasure. This study investigated the dynamic characteristics of batteryless magnetostrictive alloys for energy harvesting to detect human coronavirus 229E (HCoV-229E). Light and thin magnetostrictive Fe-Co/Ni clad plate with rectification, direct current (DC) voltage storage capacitor, and wireless information transmission circuits were developed for this purpose. The power consumption was reduced by improving the energy storage circuit, and the magnetostrictive clad plate under bending vibration stored a DC voltage of 1.9 V and wirelessly transmitted a signal to a personal computer once every 5 min and 10 s under bias magnetic fields of 0 and 10 mT, respectively. Then, on the clad plate surface, a novel CD13 biorecognition layer was immobilized using a self-assembled monolayer of -COOH groups, thus forming an amide bond with -NH2 groups for the detection of HCoV-229E. A bending vibration test demonstrated the resonance frequency changes because of HCoV-229E binding. The fluorescence signal demonstrated that HCoV-229E could be successfully detected. Thus, because HCoV-229E changed the dynamic characteristics of this plate, the CD13-modified magnetostrictive clad plate could detect HCoV-229E from the interval of wireless communication time. Therefore, a monitoring system that transmits/detects the presence of human coronavirus without batteries will be realized soon.

    DOI: 10.1016/j.sna.2022.114052

    PubMed

  • Batteryless wireless magnetostrictive Fe30Co70/Ni clad plate for human coronavirus 229E detection Reviewed

    Daiki Neyama, Siti Masturah binti Fakhruddin, Kumi Y. Inoue, Hiroki Kurita, Shion Osana, Naoto Miyamoto, Tsuyoki Tayama, Daiki Chiba, Masahito Watanabe, Hitoshi Shiku, Fumio Narita.

    SENSORS AND ACTUATORS A-PHYSICAL   349 ( 1 )   114052   2022.11( ISSN:0924-4247 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1016/j.sna.2022.114052

  • High-sensitivity amperometric dual immunoassay using two cascade reactions with signal amplification of redox cycling in nanoscale gap Reviewed

    Kentaro Ito Kentaro Ito Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki Aoba, Sendai, Miyagi 980-8579, Japan More by Kentaro Ito , Kumi Y Inoue*, Kosuke Ino, and Hitoshi Shiku*

    ANALYTICAL CHEMISTRY   94 ( 47 )   16451 - 16460   2022.11( ISSN:0003-2700  eISSN:1520-6882 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    Here, we report a high-sensitivity dual immunoassay using Lumulus amebocyte lysate (LAL) and blood coagulation cascade reactions with redox cycling in a nanoscale-gap electrode. Endotoxin and factor XIa were used as the label molecules for the immunoassay of two types of analytes to induce the LAL and coagulation cascade reactions, respectively, when each corresponding analyte existed in the sample solution. In addition to the signal amplification by the cascade reactions, we employed redox cycling in a nanoscale gap to achieve a highly sensitive assay. The nanoscale-gap electrode amplifies the amperometric signals from p-aminophenol liberated from artificial substrates in the final steps of the cascade reactions. First, the cross reaction between the LAL and coagulation cascade reactions was investigated. The results indicated that these cascade reactions did not efficiently proceed in a single solution owing to the cross reaction. Therefore, we selected to induce two cascade reactions in different solutions by bisecting the beads after the immunocomplex formation on the beads. The cross reactions of factor XIa with the LAL cascade reaction and of endotoxin with the coagulation cascade reaction were investigated. The effects of these cross reactions were revealed to be negligible by bisecting the beads before inducing the cascade reactions. Finally, a dual immunoassay for goat and human immunoglobulin G was performed, for which the limits of detection were 70 pg/mL (470 fmol/L) and 1.0 ng/mL (6.6 pmol/L), respectively. Thus, our dual immunoassay provides a sensitive platform for clinical diagnosis requiring detection of multiple analytes.

    DOI: 10.1021/acs.analchem.2c03921

    PubMed

  • Surface modification of pH-sensitive ISFET to use as aptasensor

    Kumi Y. Inoue, Siti Masturah binti Fakhruddin, Masayoshi Esashi, Hitoshi Shiku.

    Chemical Sensors   38 ( Suppl. B )   19 - 21   2022.9

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (research society, symposium materials, etc.)  

  • Surface modification of pH-sensitive ISFET to use as aptasensor

    Kumi Y. Inoue, Siti Masturah, binti Fakhruddin, Masayoshi Esashi, Hitoshi Shiku

    Chemical Sensors   38 ( Suppl. B )   19 - 21   2022.9

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • Highly Sensitive Electrochemical Immunoassay Using Signal Amplification of the Coagulation Cascade Reviewed

    Kentaro Ito Kentaro Ito Graduate School of Environmental Studies, Tohoku University, 6-6-11-604 Aramaki Aoba, Sendai, Miyagi 980-8579, Japan More by Kentaro Ito , Kumi Y. Inoue*, Kosuke Ino, and Hitoshi Shiku*

    ANALYTICAL CHEMISTRY   94 ( 36 )   12427 - 12434   2022.8( ISSN:0003-2700  eISSN:1520-6882 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    Here, we report a highly sensitive immunoassay for human immunoglobulin G (IgG) that uses signal amplification of the coagulation cascade. Z-Phe-Pro-Lys-p-nitroaniline (FPK-pNA) was used as a substrate for thrombin activation in the last step of the coagulation cascade. During the coagulation cascade, pNA is liberated from FPK-pNA and can be detected electrochemically. Using square wave voltammetry with a glassy carbon electrode, we demonstrated that pNA can be quantified in a solution modeling the coagulation cascade prepared by mixing FPK-pNA and pNA. Characterization of the reactivity of thrombin toward FPK-pNA revealed that thrombin efficiently reacted with FPK-pNA. Subsequent characterization of factor XIa activity of factor XIa-labeled antibody revealed that factor XIa was not inactivated during labeling. Finally, a coagulation cascade-based immunoassay for human IgG was performed using a factor XIa-labeled antibody on magnetic beads. The limit of detection for human IgG was 5.0 pg/mL (33 fM) indicating that the coagulation cascade can amplify the immunoassay sensitivity compared to immunoassay using a thrombin-labeled antibody as a condition without a coagulation cascade. Coagulation cascade-based immunoassay was also highly selective. In the near future, we will report a highly sensitive immunoassay for the simultaneous detection of multiple analytes using a coagulation cascade-based immunoassay and Limulus amebocyte lysate reaction-based immunoassay we previously reported.

    DOI: 10.1021/acs.analchem.2c02241

    PubMed

  • Detection of virus-like particles using magnetostrictive vibration energy harvesting Reviewed

    Hiroki Kurita, Siti Masturah Binti Fakhruddin, Daiki Neyama, Kumi Y. Inoue, Tsuyoki Tayama, Daiki Chiba, Masahito Watanabe, Hitoshi Shiku, Fumio Narita

    SENSORS AND ACTUATORS A-PHYSICAL   345 ( 1 )   113814 - 113814   2022.8( ISSN:0924-4247 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.sna.2022.113814

  • Fabrication of High-Density Vertical Closed Bipolar Electrode Arrays by Carbon Paste Filling Method for Two-Dimensional Chemical Imaging Reviewed

    Tomoki Iwama, Kumi Y. Inoue, and Hitoshi Shiku

    ANALYTICAL CHEMISTRY   94 ( 25 )   8857 - 8866   2022.6( ISSN:0003-2700  eISSN:1520-6882 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society  

    In this study, a carbon paste filling method was proposed as a simple strategy for fabricating high-density bipolar electrode (BPE) arrays for bipolar electrochemical microscopy (BEM). High spatiotemporal resolution imaging was achieved using the fabricated BPE array. BEM, which is an emerging microscopic system in recent years, achieves label-free and high spatiotemporal resolution imaging of molecular distributions using high-density BPE arrays and electrochemiluminescence (ECL) signals. We devised a simple method to fabricate a BPE array by filling a porous plate with carbon paste and succeeded in fabricating a high-density BPE array (15 μm pitch). After a detailed observation of the surface of the BPE array using a scanning electron microscope, the basic electrochemical and ECL emission characteristics were evaluated using potassium ferricyanide solution as a sample solution. Moreover, inflow imaging of the sample molecules was conducted to evaluate the imaging ability of the prepared BPE array. In addition, Prussian Blue containing carbon ink was applied to the sample solution side of the BPE array to provide catalytic activity to hydrogen peroxide, and the quantification and inflow imaging of hydrogen peroxide by ECL signals was achieved. This simple fabrication method of the BPE array can accelerate the research and development of BEM. Furthermore, hydrogen peroxide imaging by BEM is an important milestone for achieving bioimaging with high spatiotemporal resolution such as biomolecule imaging using enzymes.

    DOI: 10.1021/acs.analchem.1c05354

    PubMed

  • Dopamine detection with bipolar electrochemical microscopy using cathodic luminophore.

    Kumi Y. Inoue, Mayo Komatsu, Tomoki Iwama and Hitoshi Shiku.

    Chemical Sensors   38 ( Suppl. A )   85 - 87   2022.3

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (research society, symposium materials, etc.)  

  • Electrochemical multiplex immunoassay with dual signal amplification systems of cascade reaction and redox cycling

    Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku

    Chemical Sensors   38 ( Suppl. A )   73 - 75   2022.3

     More details

    Language:Japanese   Publishing type:Research paper (research society, symposium materials, etc.)  

  • Electrochemical Substrates and Systems for Enzyme-Based Bioassays Reviewed

    Yoshinobu UTAGAWA, Kentaro ITO, Kumi Y. INOUE, Yuji NASHIMOTO, Kosuke INO, Hitoshi SHIKU

    BUNSEKI KAGAKU   71 ( 3 )   109 - 117   2022.3( ISSN:0525-1931 )

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Analytical Chemistry  

    DOI: 10.2116/bunsekikagaku.71.109

  • Electrochemical multiplex immunoassay with dual signal amplification systems of cascade reaction and redox cycling

    Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Hitoshi Shiku

    Chemical Sensors   38 ( Suppl. A )   73 - 75   2022.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • Dopamine detection with bipolar electrochemical microscopy using cathodic luminophore

    Kumi Y. Inoue, Mayo Komatsu, Tomoki Iwama, Hitoshi Shiku

    Chemical Sensors   38 ( Suppl. A )   85 - 87   2022.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • バイポーラ電極によるバイオセンシング・バイオイメージング

    井上 久美

    近未来のデジタルヘルスを支える酵素バイオ技術~センサと発電~   2022.2

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)  

  • Electrochemiluminescence imaging based on bipolar electrochemistry using commercially available anisotropic conductive films. Reviewed

    Rise Akasaka, Kosuke Ino*, Tomoki Iwama, Kumi Y. Inoue, Yuji Nashimoto, Hitoshi Shiku*

    SENSORS AND MATERIALS   34 ( 8 )   3113 - 3113   2022.1( ISSN:0914-4935  eISSN:2435-0869 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MYU K.K.  

    DOI: 10.18494/sam3745

  • Thermally-drawn multi-electrode fibers for bipolar electrochemistry and magnified electrochemical imaging Reviewed

    Tomoki Iwama, Yuanyuan Guo, Shoma Handa, Kumi Y. Inoue*, Tatsuo Yoshinobu, Fabien Sorin, Hitoshi Shiku*

    Advanced Materials Technologies   2021.11( ISSN:2365-709X )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Thermally‐Drawn Multi‐Electrode Fibers for Bipolar Electrochemistry and Magnified Electrochemical Imaging Reviewed

    Tomoki Iwama, Yuanyuan Guo, Shoma Handa, Kumi Y. Inoue, Tatsuo Yoshinobu, Fabien Sorin, Hitoshi Shiku

    Advanced Materials Technologies   7 ( 5 )   2101066 - 2101066   2021.11( ISSN:2365-709X )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    Imaging systems using closed bipolar electrode (cBPE) arrays and electrochemiluminescence (ECL) have attracted great attention in recent years as a 2D imaging platform with high spatiotemporal resolution. However, the fabrication techniques for cBPE arrays involve complicated procedures. Therefore, a new fabrication scheme enabling the mass production of cBPE arrays with high precision, reproducibility, and yield, is desired. Here, the use of a versatile and scalable thermal drawing process as a novel fabrication method for fiber-based cBPEs with feature sizes down to micro-/nanoscales is proposed. First, a single-electrode fiber consisting of a carbon-based composite as the electrode material is produced by thermal drawing. The fundamental electrical properties of the single-electrode fiber are characterized, and its applicability to the cBPE-ECL system is demonstrated. A multielectrode fiber is fabricated by subjecting a bundle of 104 single-electrode fibers to thermal drawing. Its usability as a cBPE array for ECL imaging is confirmed with a functional rate of 99%. Further the multielectrode fiber, utilizing the principle of thermal drawing, for magnified electrochemical imaging is tapered. This work establishes a novel mass-production method for cBPE arrays, as well as a proof of concept for magnified electrochemical imaging using a thermally-drawn electrode array fiber.

    DOI: 10.1002/admt.202101066

    Web of Science

  • Series: That's the Thing in Electroanalytical Chemistry! Part 8; Masahiro Yamamoto (ed) "When Connecting Batteries in Series"

    Kumi Y. Inoue,Hirosuke Tatsumi

    Review of Polarography   67 ( 2 )   83 - 84   2021.10( ISSN:0034-6691 )

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)  

  • That’s the Thing in Electroanalytical Chemistry! (Part 8) Reviewed

    Masahiro Yamamoto(ed), KY Inoue, Hirosuke Tatsumi

    Review of Polarography   67 ( 2 )   83 - 84   2021.10( ISSN:0034-6691  eISSN:1884-7692 )

     More details

    Language:Japanese   Publisher:The Polarographic Society of Japan  

    DOI: 10.5189/revpolarography.67.83

  • Electrochemical Immunoassay with Dual-Signal Amplification for Redox Cycling within a Nanoscale Gap Reviewed

    Kentaro Ito, Kumi Y. Inoue*, Takahiro Ito-Sasaki, Kosuke Ino, and Hitoshi Shiku*

    ACS Applied Nano Materials   4 ( 11 )   12393 - 12400   2021.10( ISSN:2574-0970 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Electrochemical Immunoassay with Dual-Signal Amplification for Redox Cycling within a Nanoscale Gap Reviewed

    Kentaro Ito, Kumi Y. Inoue, Takahiro Ito-Sasaki, Kosuke Ino, Hitoshi Shiku

    ACS Applied Nano Materials   4 ( 11 )   12393 - 12400   2021.10( ISSN:2574-0970  eISSN:2574-0970 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society (ACS)  

    Here, we report a highly sensitive and easy-to-use electrochemical immunoassay using a dual-signal amplification strategy of redox cycling within a nanoscale gap and the Limulus amebocyte lysate (LAL) cascade reaction. After the antigen-antibody reaction using an endotoxin-labeled antibody, the LAL reaction was induced by endotoxin as the first signal amplification. Subsequently, p-aminophenol (pAP) liberated from peptideconjugated pAP (Boc-Leu-Gly-Arg-pAP; LGR-pAP), at the last step of the LAL reaction, was detected with redox cycling within a nanoscale gap using a fabricated device as the second signal amplification. First, an electrochemical immunoassay for goat immunoglobulin G (IgG), used as a model analyte, was performed with an endotoxin-labeled antibody using a 96-well plate. The results indicated that the endotoxin-labeled antibody was usable for immunoassays. However, a high background was observed. To decrease the background and increase the efficiency of the reactions, magnetic beads in an endotoxin-free test tube were used for the immunosorbent assay. After optimization of the condition, goat IgG was successfully detected with single-signal amplification of the cascade reaction at concentrations as low as 10 pg/mL (67 fM) using a Au disc electrode. Finally, an immunoassay for goat IgG on magnetic beads using a redox cycling-inducing device was performed. With the dual-signal amplification strategies of redox cycling within a nanoscale gap and LAL reactions, the detection limit was 70 fg/mL (470 aM). Our highly sensitive and easy-to-use immunoassay strategy provides a platform for clinical diagnosis to detect early-stage infections.

    DOI: 10.1021/acsanm.1c02837

    Web of Science

  • Cover Feature: Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore (ChemElectroChem 18/2021) Reviewed

    Tomoki Iwama, Mayo Komatsu, Kumi Y. Inoue, Hitoshi Shiku

    ChemElectroChem   8 ( 18 )   3428 - 3428   2021.9

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/celc.202101037

  • Simultaneous Monitoring of Oxygen Consumption and Movement of Zebrafish Embryos Based on an LSI-based Electrochemical Multiple-biosensor Reviewed

    Masato Suzuki, Yuka Iwaki, Kazuki Terao, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Tomoyuki Yasukawa*

    BUNSEKI KAGAKU   70 ( 9 )   535 - 540   2021.9( ISSN:0525-1931 )

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Cover Feature: Detection and 2D Imaging of Dopamine Distribution Using a Closed Bipolar Electrode System by Applying a Cathodic Luminophore (ChemElectroChem 18/2021) Reviewed

    Tomoki Iwama, Mayo Komatsu, Kumi Y. Inoue, Hitoshi Shiku

    ChemElectroChem   8 ( 18 )   3428 - 3428   2021.8( ISSN:2196-0216 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1002/celc.202101037

  • Detection and 2D imaging of dopamine distribution using a closed bipolar electrode system by applying cathodic luminophore. Reviewed

    omoki Iwama, Mayo Komatsu, Kumi Y. Inoue* and Hitoshi Shiku*

    ChemElectroChem   8 ( 18 )   3492 - 3498   2021.6( ISSN:2196-0216  eISSN:2196-0216 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    A 2D imaging system using closed bipolar electrodes (cBPEs) and electrochemiluminescence (ECL) is expected to realize high spatio-temporal resolution imaging. However, imaging of the distributions of molecules that cause an oxidation reaction on the electrode surface was not achieved by the cBPE/ECL system because the conventionally used luminophore can generate ECL only by an oxidation reaction. In this paper, we propose to implement a cathodic luminophore, [Ru(bpy)(3)]Cl-2/glutathione disulfide (GSSG) producing ECL by reduction reaction into the cBPE/ECL system to enable detection and 2D imaging of dopamine, which is an oxidizable redox species and an important neurotransmitter. The applicability of [Ru(bpy)(3)]Cl-2/GSSG for dopamine detection using a single cBPE was shown and its electrochemical properties were discussed. In addition, the demonstration of the 2D imaging of dopamine was performed using a cBPE array. These results show the potential to greatly expand the applicability of detection and imaging systems using cBPE/ECL.

    DOI: 10.1002/celc.202100675

    Web of Science

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/celc.202100675

  • Electrochemical sensor to detect proteinuria using peptidases and glutamate oxidase jointly immobilized on a prussian blue-modified electrode Reviewed

    Kentaro Ito, Kumi Y. Inoue*, Tsubasa Miura, Tomokazu Matuse, and Hitoshi Shiku*

    ELECTROCHEMISTRY   89 ( 5 )   409 - 414   2021.5( ISSN:1344-3542  eISSN:2186-2451 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Electrochemical Society of Japan  

    In this work, we report a simple electrochemical sensor that detects proteinuria upon immersion into a sample. We immobilized peptidases in conjunction with glutamate oxidase (GluOx) on a Prussian bluemodified glassy carbon (PB-GC) electrode. In the sensor, albumin, which is a major protein in urine, was degraded by peptidases to produce glutamate that was detected by GluOx on PB-GC. Initially, we validated the strategy for the detection of proteinuria using glutamate sensing. We quantified the amount of glutamate produced by degrading human serum albumin with HCI and carboxypeptidase A (CPA). The results indicated that an increase in the enzyme degradation rate was necessary for daily proteinuria sensing that needs to be completed within 1 h. Therefore, we investigated the use of endopeptidase in conjunction with CPA. The combination of proteinase K and CPA liberated glutamate from albumin at 17.5 times higher comparing to solely CPA. The subsequent glutamate assay using peptidase-modified GluOxPB-GC electrode did not exhibit changes in the electrochemical signal with increases in the glutamate concentration, because peptidase degraded the GluOx on the modified electrode. To prevent this, GluOx was encapsulated in ZIF-8. Glutamate was successfully detected using peptidase and GluOx encapsulated in the ZIF-8-modified PB-GC (peptidase-ZIF-8/GluOx-PB-GC) electrode. Finally, an albumin assay was performed using the peptidase-ZIF-8/GluOx-PB-GC electrode, wherein albumin was detected with a sensitivity of 0.1 mg/mL within 30min. Our simple and easy-to-use method for albumin detection provides a viable sensor for daily urinalysis. (C) The Author(s) 2021. Published by ECSJ.

    DOI: 10.5796/electrochemistry.21-00058

    Web of Science

  • Bipolar electrode-based electrochromic devices for analytical applications -- a Review Invited Reviewed

    Siti Masturah binti Fakhruddin, Kosuke Ino,* Kumi Y. Inoue, Yuji Nashimoto and Hitoshi Shiku*

    ELECTROANALYSIS   34 ( 2 )   212 - 226   2021.5( ISSN:1040-0397  eISSN:1521-4109 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    Bipolar electrode-based (BPE-based) electrochromic devices have garnered increasing attention in the past decade. These BPE-based electrochromic devices have been used for analytical health monitoring, point-of-care (POC) diagnostics, and chemical sensing. In this review, we highlight recent progress made regarding BPE-based electrochromic devices constructed for these analytical applications. Various, available electrochromic materials are summarized in the first section, after which the different device types (e. g., paper-based and self-powered) are discussed. Biological- and chemical-based analytical demonstrations of these devices are then reviewed. Finally, we conclude this review with a perspective on the future developments of BPE-based electrochromic devices in analytical applications.

    DOI: 10.1002/elan.202100153

    Web of Science

  • Development of biosensors using bipolar electrode Invited

    Kumi Y. Inoue

    Monthly Bioindustry   38 ( 5 )   57 - 67   2021.5

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (commerce magazine)   Publisher:CMC Publishing  

  • バイポーラ電極を用いるバイオセンサの開発 Invited

    井上(安田)久美

    Bio INDUSTRY (シーエムシー出版)   38 ( 5 )   57 - 67   2021.5

     More details

    Language:Japanese  

  • Simultaneous Monitoring of Oxygen Consumption and Movement of Zebrafish Embryos Based on an LSI-based Electrochemical Multiple-biosensor Reviewed

    Masato Suzuki, Yuka Iwaki, Kazuki Terao, Ryota Kunikata, Atsushi Suda, Kumi Y. Inoue, Kosuke Ino, Tomokazu Matsue, Tomoyuki Yasukawa

    BUNSEKI KAGAKU   70 ( 9 )   535 - 540   2021( ISSN:0525-1931 )

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC ANALYTICAL CHEMISTRY  

    We have estimated the effect of a chemical reagent based on the oxygen consumption and the motion of zebrafish embryos by using an LSI-based amperometric sensor array device (BioLSI). Bio-LSI with 400 gold microelectrodes in a 6 mmx6 mm region enabled us to visualize the distribution of the concentration of redox species as images. We applied -0.2 V vs. Ag/AgCl to all electrodes of a Bio-LSI chip to obtain the distribution of the oxygen concentrations in the presence of zebrafish embryos. The motions of the embryos were observed and traced by a CMOS camera. The reduction currents, obtained at the electrodes immediately under the embryos, are smaller than that obtained at the electrodes without the embryos. When the embryos moved to the position above other electrodes, the positions of electrodes with the low reduction current synchronized to the position of the embryos. This was due to the consumption of oxygen by the respiration of embryos and the inhibition of the diffusion of oxygen to the electrodes. When a solution containing 2,4-dinitrophenol (DNP), that is an uncoupler of oxidative phosphorylation, was added, the reduction current drastically decreased and then gradually returned to the original level. The active motions of the embryos coupled with the decrease of the reduction current were observed immediately after adding DNP, while no motion was observed after turning toward original level. Embryos would transiently enhance the consumption of oxygen by respiration due to the compensation of its depression of ATP synthesis, and then be led to death. The present system based on the reduction currents of oxygen and the motions of embryos would provide simple and high-throughput toxicity tests for various chemical agents.

    Web of Science

  • デジタルヘルス時代のバイオセンサ・バイオセンシング Reviewed

    井上 久美, 大石 哲雄

    電気化学   88 ( 4 )   298 - 298   2020.12( ISSN:2433-3255 )

     More details

    Authorship:Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)  

    DOI: 0.5796/denkikagaku.20-FE0026

  • Closed Bipolar Electrochemistry for Sensor and Bioimaging Applications Invited Reviewed

    KY Inoue, T Iwama, SM Fakhruddin, H Shiku, T Matsue

    ECS Meeting Abstracts   44   2811 - 2811   2020.11

     More details

    Authorship:Lead author   Language:English   Publishing type:(MISC) Summary of the papers read (international conference)  

  • (Invited) Closed Bipolar Electrochemistry for Sensor and Bioimaging Applications

    Kumi Y. Inoue, Tomoki Iwama, Siti Masturah Fakhruddin, Hitoshi Shiku, Tomokazu Matsue

    ECS Meeting Abstracts   2020.11

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:(MISC) Summary of the papers read (international conference)  

    DOI: 10.1149/MA2020-02442811mtgabs

  • 単一および局所計測技術と電気化学の接点 Reviewed

    池田勝佳, 井上久美

    電気化学   88 ( 3 )   209 - 209   2020.9( ISSN:2433-3255 )

     More details

    Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)  

    DOI: 0.5796/denkikagaku.20-FE0026

  • Bioimaging using bipolar electrochemical microscopy improved in spatial resolution Reviewed

    Tomoki Iwama,Kumi Y. Inoue,Hiroya Abe,Tomokazu Matsue,Hitoshi Shiku

    ANALYST   145 ( 21 )   6895 - 6900   2020.7( ISSN:0003-2654  eISSN:1364-5528 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry (RSC)  

    <p>In this study, we developed a bipolar electrochemical microscopy (BEM) using a closed bipolar electrode (cBPE) array with an electrochemiluminescence (ECL) detecting system. Because cBPEs are not directly connected to...</p>

    DOI: 10.1039/d0an00912a

    Web of Science

    Scopus

    PubMed

  • Recent Advances in Electrochemiluminescence-Based Systems for Mammalian Cell Analysis. Reviewed

    Kaoru Hiramoto,Elena Villani,Tomoki Iwama,Keika Komatsu,Shinsuke Inagi,Kumi Y Inoue,Yuji Nashimoto,Kosuke Ino,Hitoshi Shiku

    Micromachines   11 ( 5 )   2020.5(  eISSN:2072-666X )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI  

    Mammalian cell analysis is essential in the context of both fundamental studies and clinical applications. Among the various techniques available for cell analysis, electrochemiluminescence (ECL) has attracted significant attention due to its integration of both electrochemical and spectroscopic methods. In this review, we summarize recent advances in the ECL-based systems developed for mammalian cell analysis. The review begins with a summary of the developments in luminophores that opened the door to ECL applications for biological samples. Secondly, ECL-based imaging systems are introduced as an emerging technique to visualize single-cell morphologies and intracellular molecules. In the subsequent section, the ECL sensors developed in the past decade are summarized, the use of which made the highly sensitive detection of cell-derived molecules possible. Although ECL immunoassays are well developed in terms of commercial use, the sensing of biomolecules at a single-cell level remains a challenge. Emphasis is therefore placed on ECL sensors that directly detect cellular molecules from small portions of cells or even single cells. Finally, the development of bipolar electrode devic

    DOI: 10.3390/mi11050530

    Web of Science

    PubMed

  • Electrochemical Bioimaging Platform Using Closed Bipolar Electrode Array Invited

    Kumi Y Inoue, Tomoki Iwama, Siti Masturah Fakhruddin, Hitoshi Shiku, Tomokazu Matsue

    ECS Meeting Abstracts   33   2372 - 2372   2020.5

     More details

    Authorship:Lead author   Language:English   Publishing type:(MISC) Summary of the papers read (international conference)  

  • Electrochemical Bioimaging Platform Using Closed Bipolar Electrode Array

    Kumi Y. Inoue, Tomoki Iwama, Siti Masturah Fakhruddin, Hitoshi Shiku, Tomokazu Matsue

    ECS Meeting Abstracts   2020.5

     More details

    Language:English  

    DOI: 10.1149/MA2020-01332372mtgabs

  • Highly sensitive detection of endotoxin using redox cycling with nanogap electrodes.

    Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue and Hitoshi Shiku

        2020.3

     More details

    Language:Japanese   Publishing type:Research paper (research society, symposium materials, etc.)  

  • Development of the bipolar electrochemical microscopy and its demonstration for high spatio-temporal resolution bio-imaging application

    Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku

    Chemical Sensors   36 ( Suppl. A )   58 - 60   2020.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • Electrochemical Detection of Kallikrein Using a p-Methoxyaniline-conjugated Tripeptide towards Simple Diagnosis of Primary Aldosteronism Reviewed

    Kousuke Ohyama,Sun Sixiang,Kumi Y. Inoue,Tomokazu Matsue,Takayuki Doi

    CHEMISTRY LETTERS   49 ( 1 )   57 - 59   2020.1( ISSN:0366-7022  eISSN:1348-0715 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Chemical Society of Japan  

    Kallikrein is a serine protease which is involved in blood pressure regulation and is overexpressed in primary aldosteronism. We hypothesized that quick quantification of this enzyme could represent a valuable diagnostic technique. Towards this aim, we demonstrate the use of an electrochemical approach to detect and quantify kallikrein for the first time. We designed the tripeptide Pro-Phe-Arg-pMA as a substrate for kallikrein and analyzed the enzyme concentration by measuring the release of the product, p-methoxyaniline (pMA), from kallikrein-mediated hydrolysis. Released pMA was detected by electrochemical measurement with a detection limit of 0.01 units/mL. The fit of the analysis of released pMA by chronoamperometry with Michaelis-Menten curves indicates that this technique offers quantitative detection.

    DOI: 10.1246/cl.190729

    Web of Science

  • バイオLSIを用いたゼブラフィッシュ胚周辺の酸素濃度計測に基づく毒性試験法の開発

    寺尾和輝, 鈴木雅登, 國方亮太, 須田篤史, 井上(安田)久美, 伊野浩介, 末永智一, 安川智之

    分析化学討論会講演要旨集(Web)   80th   2020

     More details

    Language:Japanese  

    J-GLOBAL

  • Real-time imaging of photosynthetic oxygen evolution from spinach using LSI-based biosensor Reviewed

    Shigenobu Kasai,Yamato Sugiura,Ankush Prasad,Kumi Y. Inoue,Teruya Sato,Tomohiro Honmo,Aditya Kumar,Pavel Pospíšil,Kosuke Ino,Yuka Hashi,Yoko Furubayashi,Masahki Matsudaira,Atsushi Suda,Ryota Kunikata,Tomokazu Matsue

    Scientific Reports   9 ( 1 )   12234 - 12234   2019.8( ISSN:2045-2322  eISSN:2045-2322 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    The light-driven splitting of water to oxygen (O2) is catalyzed by a protein-bound tetra-manganese penta-oxygen calcium (Mn4O5Ca) cluster in Photosystem II. In the current study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to perform two-dimensional imaging of light-induced O2 evolution from spinach leaves. The employed Bio-LSI chip consists of 400 sensor electrodes with a pitch of 250 μm for fast electrochemical imaging. Spinach leaves were illuminated to varying intensities of white light (400-700 nm) which induced oxygen evolution and subsequent electrochemical images were collected using the Bio-LSI chip. Bio-LSI images clearly showed the dose-dependent effects of the light-induced oxygen release from spinach leaves which was then significantly suppressed in the presence of urea-type herbicide 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU). Our results clearly suggest that light-induced oxygen evolution can be monitored using the chip and suggesting that the Bio-LSI is a promising tool for real-time imaging. To the best of our knowledge, this report is the first to describe electrochemical imaging of light-induced O2 evolution using LSI-based amperometric sensors in plants.

    DOI: 10.1038/s41598-019-48561-y

    PubMed

    Other Link: http://www.nature.com/articles/s41598-019-48561-y

  • A Nanopore-based Endotoxin Sensor Reviewed

    Kentaro Ito,Kumi Y. Inoue,Takahiro Ito-Sasaki,Tomokazu Matsue,Hitoshi Shiku

    BUNSEKI KAGAKU   68 ( 8 )   575 - 580   2019.8( ISSN:0525-1931 )

     More details

    Authorship:Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  • A Nanopore-based Endotoxin Sensor Reviewed

    Kentaro ITO, Kumi Y. INOUE, Takahiro ITO-SASAKI, Tomokazu MATSUE, Hitoshi SHIKU

    BUNSEKI KAGAKU   68 ( 8 )   575 - 580   2019.8( ISSN:0525-1931 )

     More details

    Authorship:Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Japan Society for Analytical Chemistry  

    We report a rapid and simple method to detect endotoxin using a nanopore sensor based on the Coulter principle. An ion current momentary decreases when an endotoxin micelle translocates through the nanopore at a tip of the glass capillary with the voltage biased across the pore. Quantitative detection is available by counting pulsed currents appear within a limited period. First, we characterized the nanopores of fabricated glass capillaries using a scanning electron microscope. The size of the pore at the tip of the glass capillary was 260 nm. Next, endotoxin assays using a nanopipette were performed. An Ag/AgCl wire inserted into a glass capillary filled with phosphate buffer saline (PBS) containing endotoxin was biased at 1 V versus another Ag/AgCl wire inserted in a bulk solution containing endotoxin-free PBS. No pulsed current was observed during 4 min of monitoring when the endotoxin concentrations in the glass capillary were below 100 Endotoxin Unit (EU) L-1.When 500- 10000 EU L-1 was in the glass capillary, pulsed currents were observed with an increase of the pulse height and pulse frequency with increasing of the endotoxin concentration. This is because the size of the endotoxin micelles as well as the micelle concentration were increased with the increase of the endotoxin concentration. The fact that pulses appeared far less frequently than expected indicates that only large micelles associated with each other were observed as a pulsed current. Although the current detection limit is 500 EU L-1. optimization of the pore diameter will make it possible to detect even smaller micelles, resulting in an increased the sensitivity. This method is expected to be applied to use as a rapid and simple sensor to detect endotoxin, especially in the continuous monitoring of dialysis solution.

    DOI: 10.2116/bunsekikagaku.68.575

    Web of Science

  • Aptamer-Based Allergen Sensing System for Food Safety

    Hamza Abdelli, Takashiro Tsukamoto, Takahiro Ito, Kumi Y. Inoue, Tomokazu Matsue, Shuji Tanaka

    2019 20th International Conference on Solid-State Sensors, Actuators and Microsystems &amp; Eurosensors XXXIII (TRANSDUCERS &amp; EUROSENSORS XXXIII)   2019.6

     More details

    Language:English   Publisher:IEEE  

    DOI: 10.1109/TRANSDUCERS.2019.8808677

    Web of Science

  • A highly sensitive endotoxin sensor based on redox cycling in a nanocavity Reviewed

    Kentaro Ito,Kumi Y. Inoue,Kosuke Ino,Tomokazu Matsue,Hitoshi Shiku

    ANALYST   144 ( 11 )   3659 - 3667   2019.4( ISSN:0003-2654  eISSN:1364-5528 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Royal Society of Chemistry (RSC)  

    <p>A highly sensitive endotoxin sensor and novel analytical principle using diffusion coefficient difference was developed using a nanocavity device.</p>

    DOI: 10.1039/C9AN00478E

    Web of Science

    PubMed

  • Electrochemical urine sensor using proteases

    Kumi Y. Inoue, Tsubasa Miura, Tomokazu Matsue

    Chemical Sensors   35 ( Suppl. A )   22 - 24   2019.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • Highly sensitive detection of endotoxin using redox cycling with nanogap electrodes

    Kentaro Ito, Kumi Y. Inoue, Kosuke Ino, Yuji Nashimoto, Hitoshi Shiku

    Chemical Sensors   35 ( Suppl. A )   25 - 27   2019.3

     More details

    Language:Japanese   Publishing type:(MISC) Summary of the papers read (national conference and other science council)  

  • ヤヌス粒子を用いたアルドステロン症等の診断システムの開発

    藪浩, 前田郁麻, 岩本恭典, 佐藤さつき, 井上久美, 珠玖仁, 末永智一, 根本靖久, 佐藤文俊

    日本内分泌学会雑誌   95 ( 1 )   2019( ISSN:0029-0661 )

     More details

    Language:Japanese  

    J-GLOBAL

  • Development of a high spatio-temporal resolution electrochemical imaging system using a closed bipolar electrode array

    Tomoki Iwama, Kumi Y. Inoue, Hiroya Abe, Tomokazu Matsue, Hitoshi Shiku

    23rd International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2019   1380 - 1381   2019(  ISBN:9781733419000 )

     More details

    Language:English  

    In this study, we developed a novel electrochemical imaging system using a closed bipolar electrode (cBPE) with electrochemiluminescence (ECL) detecting system to realize the high spatio-temporal resolution imaging. This novel imaging system can obtain high spatial resolution images because each cBPEs need no connecting lines, which severely limited the spatial resolution of the conventional electrochemical images obtained by the micro-electrode array method. We successfully applied the system to monitor the spreading activity of potassium ferricyanide solution in high spatio-temporal resolution.

    Scopus

  • Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme-modified Large-scale Integration (LSI)-based Amperometric Electrochemical Device Reviewed

    Hiroya Abe,Tomoki Iwama,Hiroshi Yabu,Kosuke Ino,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Tomokazu Matsue

    ELECTROANALYSIS   30 ( 12 )   2841 - 2846   2018.12( ISSN:1040-0397  eISSN:1521-4109 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme-modified large-scale integration (LSI)-based amperometric devices, referred to “Bio-LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer-modified electrodes. The Os-HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme-modified Bio-LSI.

    DOI: 10.1002/elan.201800386

    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/elan.201800386

  • Cover Picture: Simultaneous and Selective Imaging of Dopamine and Glutamate Using an Enzyme‐modified Large‐scale Integration (LSI)‐based Amperometric Electrochemical Device (Electroanalysis 12/2018)

    Hiroya Abe, Tomoki Iwama, Hiroshi Yabu, Kosuke Ino, Kumi Y. Inoue, Atsushi Suda, Ryota Kunikata, Masahki Matsudaira, Tomokazu Matsue

    Electroanalysis   30 ( 12 )   2841 - 2846   2018.12( ISSN:1040-0397  eISSN:1521-4109 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    © 2018 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim Since neurotransmitters play critical roles in nervous systems, the imaging techniques that can visualize them are needed. This communication describes the development of a method for simultaneous imaging of dopamine (DA) and glutamate (Glu), typical neurotransmitters, using enzyme-modified large-scale integration (LSI)-based amperometric devices, referred to “Bio-LSI”. The DA was detected by using direct electrochemical oxidation while Glu was measured by using an indirect electrochemical reaction involving glutamate oxidase (GluOx)/horseradish peroxidase (HRP) and osmium (Os) polymer-modified electrodes. The Os-HRP polymer was modified on designated sensor electrodes through electrodeposition. The selective and simultaneous imaging of neurotransmitters such as DA and Glu was achieved by applying the enzyme-modified Bio-LSI.

    DOI: 10.1002/elan.201881201

    Web of Science

    Scopus

  • Development of amperometric sensor chips for medical and biological fields. Invited

    INOUE Kumi Y

    Chemical Sensors   34   58 - 68   2018.9

     More details

    Authorship:Lead author, Last author, Corresponding author   Language:Japanese  

  • Simultaneous multiplex potentiostatic electroanalysis with liquid‐junction‐removed reference electrode system using a closed bipolar electrode. Reviewed

    Kumi Y. Inoue,Miho Ikegawa,Takahiro Ito-Sasaki,Shinichiro Takano,Hitoshi Shiku,Tomokazu Matsue

    ChemElectroChem   5 ( 16 )   2167 - 2170   2018.8( ISSN:2196-0216 )

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    A novel liquid-junction-removed reference electrode system was developed for simultaneous multiplex amperometry using a closed bipolar electrode. The liquid junction between the reference cell and the sample cell was replaced by a bipolar electrode to ensure electron conductivity in lieu of ionic conductivity in conventional systems. Under the condition where the redox reaction of the analyte at the bipolar electrode in the sample cell was a limiting process, voltammetric and amperometric measurements were performed for quantitative detection of the analytes. After characterization of the system, a chip-type device was fabricated with six sample cells and one reference cell. Simultaneous determination of ferrocene methanol was conducted in six solutions in the 0.1-0.5 mM range. This is the first demonstration of the simultaneous multiplex determination of analytes with a liquid-junction-removed system. Although the potential control problem (which is different to the liquid potential problem of conventional liquid junction) still remains because of the increase of the solid-liquid interface, this system is expected to solve some problems associated with the liquid junction of the reference electrode, including sample contamination by chloride ions and the complicated process of fabricating a reference electrode on a chip.

    DOI: 10.1002/celc.201800536

    Web of Science

  • Front Cover: Simultaneous Multiplex Potentiostatic Electroanalysis with Liquid-Junction-Removed Reference Electrode System using a Closed Bipolar Electrode (ChemElectroChem 16/2018)

    Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

    ChemElectroChem   5 ( 16 )   2157 - 2157   2018.8

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/celc.201800895

  • Closed bipolar electrode system for a liquid-junction-free reference electrode integrated in an amperometric probe sensor Reviewed

    Siti Masturah binti Fakhruddin,Kumi Y. Inoue,Ryoto Tsuga,Tomokazu Matsue

    ELECTROCHEMISTRY COMMUNICATIONS   93   62 - 65   2018.8( ISSN:1388-2481  eISSN:1873-1902 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier Inc.  

    Reference electrodes (REs) play an essential role in electrochemical measurements. REs require liquid junctions to allow for ion conductivity between the RE and the sample solution. However, a liquid junction causes unwanted ion leakage into the analyte and clogging inducing reference potential inaccuracy. In this study, we report a liquid-junction-free amperometric probe sensor with an integrated RE, based on a closed bipolar electrode (cBPE) system. The liquid junction of the RE was replaced by the cBPE, which provides electrical conductivity instead of ionic conductivity between the inner solution of the RE and the sample solution. Since the solutions were in two separate cells, undesired contamination was completely prevented. We successfully conducted voltammetric and amperometric quantitative detection of 0.1–0.5 mM ferrocenemethanol using this system. Although its uses are limited, the present probe sensor with integrated liquid-junction-free reference electrode provides a feasible alternative for amperometric electrochemical measurements.

    DOI: 10.1016/j.elecom.2018.06.006

    Web of Science

    Scopus

  • Simultaneous Multiplex Potentiostatic Electroanalysis with Liquid-Junction-Removed Reference Electrode System using a Closed Bipolar Electrode

    Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

    CHEMELECTROCHEM   5 ( 16 )   2161 - 2161   2018.8( ISSN:2196-0216 )

     More details

    Language:English   Publishing type:(MISC) Other article   Publisher:WILEY-V C H VERLAG GMBH  

    The front cover artwork is provided by Prof. K. Y. Inoue, Prof. T. Matsue, and Mr. T. Iwama from Tohoku University, Sendai (Japan). The image shows an electrochemical measuring device with six sample cells and one reference cell. Simultaneous multiplex amperometry and voltammetry are available with integrated reference system without a liquid junction.

    DOI: 10.1002/celc.201800894

    Web of Science

  • ゼブラフィッシュ胚の酸素消費量に基づく化学物質のリスク評価法

    鈴木 雅登, 寺尾 和輝, 國方 亮太, 須田 篤史, 井上 久美[安田], 伊野 浩介, 末永 智一, 安川 智之

    日本分析化学会講演要旨集   67年会   97 - 97   2018.8

     More details

    Language:Japanese   Publisher:(公社)日本分析化学会  

  • ゼブラフィッシュ胚の酸素消費量に基づく化学物質のリスク評価法

    鈴木 雅登, 寺尾 和輝, 國方 亮太, 須田 篤史, 井上 久美[安田], 伊野 浩介, 末永 智一, 安川 智之

    日本分析化学会講演要旨集   67年会   97 - 97   2018.8

     More details

    Language:Japanese   Publisher:(公社)日本分析化学会  

  • Enzyme printed enzyme electrode sensor for low concentration lactate monitoring Reviewed

    Noriko Tsuruoka,Tadao Matsunaga,Kumi Y. Inoue,Tomokazu Matsue,Yoichi Haga

    IEEJ Transactions on Sensors and Micromachines   138 ( 6 )   231 - 240   2018.6( ISSN:1341-8939 )

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Institute of Electrical Engineers of Japan  

    In this research, enzyme electrode sensor for measuring low concentration lactate was fabricated. In our previous work, microperfusion needle to measure concentration of lactate in subepidermal tissue have been developed. Using this microperfusion needle, lactate concentration of perfusate was about one percent of lactate concentration of blood. In order to measure this low concentration of lactate, enzyme electrode sensor using printed enzyme was fabricated. Au electrodes were patterned on the glass substrate. Ag nanometal ink was printed on reference electrode. Os-HRP polymer was printed by pasting needle type printer and lactate oxidase was printed by inkjet printer on working electrode. Addition agents which do not inactivate enzymes as wetting agent and viscosity modifier were selected. A flow channel was pasted on fabricated electrodes to measure concentration changes continuously. Fabricated sensor had high sensitivity and could measure low concentration lactate (0.01-0.25 mM). In addition, fabricated sensor was not affected by interfering substances.

    DOI: 10.1541/ieejsmas.138.231

    Scopus

  • Chemical imaging using a closed bipolar electrode array Reviewed

    Tomoki Iwama,Kumi Y. Inoue,Hiroya Abe,Tomokazu Matsue

    CHEMISTRY LETTERS   47 ( 7 )   843 - 845   2018.5( ISSN:0366-7022  eISSN:1348-0715 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:CHEMICAL SOC JAPAN  

    © 2018 The Chemical Society of Japan. In this study, we developed a new chemical imaging platform that uses a closed bipolar electrode (cBPE) array for electrochemiluminescence (ECL) detection. The cBPE array was successfully applied to use in the chemical imaging of molecular distributions by injection and a self-induced redox cycling. The results show the possibility of high-resolution chemical imaging using a cBPE array.

    DOI: 10.1246/cl.180303

    Web of Science

    Scopus

  • 神経伝達物質の選択的イメージングに向けた電気化学デバイスの開発

    阿部博弥, 岩間智紀, 藪浩, 井上(安田)久美, 國方亮太, 須田篤史, 松平昌昭, 末永智一

    日本分析化学会年会講演要旨集   67th   2018

     More details

    Language:Japanese  

    J-GLOBAL

  • Development of amperometric sensor chips for medical and biological fields. Invited

    INOUE Kumi Y

    Chemical Sensors   34   58 - 68   2018

     More details

    Language:Japanese  

  • 原発性アルドステロン症検査のための微小粒子を用いたイムノアッセイの検討

    木内亮汰, 佐藤さつき, 伊藤健太郎, 井上久美, 藪浩, 伊野浩介, 末永智一, 珠玖仁

    日本分析化学会年会講演要旨集   67th   2018

     More details

    Language:Japanese  

    J-GLOBAL

  • Structure and electrochemical properties of nitrogen doped diamond-like carbon film synthesized by low temperature neutral beam enhanced chemical vapor deposition Reviewed

    Wang, Q.,Chang, X.,Kikuchi, Y.,Inoue, K.Y.,Kubota, T.,Matsue, T.,Nozawa, T.,Samukawa, S.

    International Journal of Electrochemical Science   13 ( 2 )   1803 - 1812   2017.12( ISSN:1452-3981 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ESG  

    We report nitrogen doping diamond-like carbon film synthesis by a new technique called neutral beam enhanced chemical vapor deposition. The deposition is processed in UV/electron damage-free environment, and the substrate temperature is controlled at room temperature. The film structure was investigated by X-ray photoelectron spectroscopy and Fourier transform infrared spectroscopy. The N/C ratio is 0.26, and doped nitrogen atoms exist in forms of amine and inside the aromatic ring, which helps increase film electrical conductivity. Cyclic voltammograms were carried out and the results indicate the DLC: N film qualified as electroanalysis sensor material.

    DOI: 10.20964/2018.02.11

    Web of Science

    Scopus

  • Electrochemicolor Imaging Using an LSI-Based Device for Multiplexed Cell Assays Reviewed

    Yusuke Kanno,Kosuke Ino,Hiroya Abe,Chika Sakamoto,Takehiro Onodera,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Hitoshi Shiku,Tomokazu Matsue

    ANALYTICAL CHEMISTRY   89 ( 23 )   12778 - 12786   2017.12( ISSN:0003-2700  eISSN:1520-6882 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER CHEMICAL SOC  

    Multiplexed bioimaging systems have triggered the development of effective assays, contributing new biological information. Although electrochemical imaging is beneficial for quantitative analysis in real time, monitoring multiple cell functions is difficult. We have developed a novel electrochemical imaging system, herein, using a large-scale integration (LSI)based amperometric device for detecting multiple biomolecules simultaneously. This system is designated as an electrochemicolor imaging system in which the current signals from two different types of biomolecules are depicted as a multicolor electrochemical image. The mode-selectable function of the 400-electrode device enables the imaging system and two different potentials can be independently applied to the selected electrodes. The imaging system is successfully applied for detecting multiple cell functions of the embryonic stem (ES) cell and the rat pheochromocytoma (PC12) cell aggregates. To the best of our knowledge, this is the first time that a real-time electrochemical mapping technique for multiple electroactive species, simultaneously, has been reported. The imaging system is a promising bioanalytical method for ex

    DOI: 10.1021/acs.analchem.7b03042

    Web of Science

    PubMed

  • Electrochemical Motion Tracking of Microorganisms Using a Large-Scale-Integration-Based Amperometric Device Reviewed

    Kosuke Ino,Yusuke Kanno,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Hitoshi Shiku,Tomokazu Matsue

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   56 ( 24 )   6818 - 6822   2017.6( ISSN:1433-7851  eISSN:1521-3773 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-V C H VERLAG GMBH  

    Motion tracking of microorganisms is useful to investigate the effects of chemical or physical stimulation on their biological functions. Herein, we describe a novel electrochemical imaging method for motion tracking of microorganisms using a large-scale integration (LSI)-based amperometric device. The device consists of 400 electrochemical sensors with a pitch of 250 mm. A convection flow caused by the motion of microorganisms supplies redox species to the sensors and increases their electrochemical responses. Thus, the flow is converted to electrochemical signals, enabling the electrochemical motion tracking of the microorganisms. As a proof of concept, capillary vibration was monitored. Finally, the method was applied to monitoring the motion of Daphnia magna. The motions of these microorganisms were clearly tracked based on the electrochemical oxidation of [Fe(CN)(6)](4-) and reduction of O-2.

    DOI: 10.1002/anie.201701541

    Web of Science

    PubMed

  • Amperometric Detection of Apoptosis by using p-Methoxyaniline-Conjugated Substrate for Caspase-3 Reviewed

    Sun Sixiang,Kumi Y. Inoue,Shusaku Shiomoto,Shinichiro Takano,Kosuke Ino,Hitoshi Shiku,Tomokazu Matsue

    ChemElectroChem   4 ( 4 )   941 - 946   2017.4( ISSN:2196-0216 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-V C H VERLAG GMBH  

    This manuscript reports on a novel substrate for the amperometric detection of cellular apoptosis, Asp-Glu-Val-Asp-p-methoxyaniline (DEVD-pMA). This substrate showed electrochemical inertia from -0.2 to 0.7V versus Ag/AgCl in phosphate-buffered saline (PBS), but was cleaved by caspase-3, releasing pMA, which was detected with cyclic voltammetry and chronoamperometry. Compared with p-aminophenol (pAP), pMA showed lower toxicity to the human hepatocellular carcinoma cell line (HepG2) and higher stability in PBS. By using this substrate, we successfully detected caspase-3 in the range of 0.31-2.5x10(-2) units/mL and cellular apoptosis in HepG2 cells by using simple amperometry without cell lysis. This work will help to develop a simple method for the electrochemical monitoring of apoptosis, which can be applied in biological research and drug development.

    DOI: 10.1002/celc.201600700

    Web of Science

  • DEVELOPMENT OF PROTEASE SUBSTRATE COMBINED WITH PHENYLENEDIAMINE DERIVATIVES AND ITS APPLICATION TO ENDOTOXIN DETECTION

    Proceedings of the Chemical Sensor Symposium   61   82 - 84   2017.3

     More details

    Language:Japanese  

  • ALIGNMENT OF NANO CARBON MATERIALS USING DIELECTROPHORESIS AND APPLICATION FOR ELECTRODE ARRAY DEVICE

    Proceedings of the Chemical Sensor Symposium   61   85 - 87   2017.3

     More details

    Language:Japanese  

  • 神経伝達物質の選択的同時イメージングに向けた電極アレイの作製

    阿部博弥, 岩間智紀, 藪浩, 伊野浩介, 井上(安田)久美, 國方亮太, 須田篤史, 松平昌昭, 末永智一

    電気化学会東北支部セミコンファレンス講演論文集   48th   2017

     More details

    Language:Japanese  

    J-GLOBAL

  • 抗原修飾ヤヌス粒子を用いたイムノアッセイ法の開発

    佐藤(増子)さつき, 井上(安田)久美, 伊藤健太郎, FAKHRUDDIN Siti Masturah, 廣瀬理美, 珠玖仁, 珠玖仁, 佐藤文俊, 藪浩, 末永智一

    日本分析化学会年会講演要旨集   66th   2017

     More details

    Language:Japanese  

    J-GLOBAL

  • Bonding state and defects of nitrogen-doped graphene in oxygen reduction reaction Reviewed

    Takeru Okada,Kumi Y. Inoue,Golap Kalita,Masaki Tanemura,Tomokazu Matsue,M. Meyyappan,Seiji Samukawa

    CHEMICAL PHYSICS LETTERS   665   117 - 120   2016.11( ISSN:0009-2614  eISSN:1873-4448 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    Nitrogen-doped graphene is favored as a catalyst for oxygen reduction reaction (ORR) over rare metals. However, the effects of bonding state, nitrogen doped site and defects on catalytic conversion are still unclear. Here, we investigate oxygen reduction reaction using nitrogen-doped graphene with selective bonding state through pyridinic and graphitic nitrogen selective approaches. Both types show ORR activity and the catalytic reaction is clarified to be a four electron reaction path. Graphitic nitrogen with a low level of defects is found superior from the viewpoint of using single graphene sheet for the ORR application. Our investigation provides useful information for various applications using doped graphene. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.cplett.2016.10.061

    Web of Science

  • Investigation of carbon-nitrogen bonding state in graphene on catalytic activity Reviewed

    T. Okada,K. Y. Inoue,T. Matsue,G. Kalita,M. Tanemura,M. Meyyappan,S. Samukawa

    17th International Conference on the science and technology nanotubes and low-dimensional materials   2016.8

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

  • Investigation of carbon-nitrogen bonding state in graphene on catalytic activity Reviewed

    T. Okada, K. Y. Inoue, T. Matsue, G. Kalita, M. Tanemura, M. Meyyappan, S. Samukawa

    17th International Conference on the science and technology nanotubes and low-dimensional materials   2016.8

     More details

    Language:English  

  • Imaging of enzyme activity using bio-LSI system enables simultaneous immunosensing of different analytes in multiple specimens Reviewed

    Toshiki Hokuto,Tomoyuki Yasukawa,Ryota Kunikata,Atsushi Suda,Kumi Y. Inoue,Kosuke Ino,Tomokazu Matsue,Fumio Mizutani

    Biotechnology Journal   11 ( 6 )   838 - 842   2016.6( ISSN:1860-6768  eISSN:1860-7314 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-V C H VERLAG GMBH  

    Electrochemical imaging is an excellent technique to characterize an activity of biomaterials, such as enzymes and cells. Large scale integration-based amperometric sensor (Bio-LSI) has been developed for the simultaneous and continuous detection of the concentration distribution of redox species generated by reactions of biomolecules. In this study, the Bio-LSI system was demonstrated to be applicable for simultaneous detection of different analytes in multiple specimens. The multiple specimens containing human immunoglobulin G (hIgG) and mouse IgG (mIgG) were introduced into each channel of the upper substrate across the antibody lines for hIgG and mIgG on the lower substrate. Hydrogen peroxide generated by the enzyme reaction of glucose oxidase captured at intersections was simultaneously detected by 400 microelectrodes of a Bio-LSI chip. The oxidation current increased with increasing the concentrations of hIgG, which can be detected in the range of 0.01-1.0 mu g mL(-1). Simultaneous detection of hIgG and mIgG in multiple specimens was achieved by using line patterns of both antibodies. Therefore, the presence of different target molecules in the multiple samples would be quant

    DOI: 10.1002/biot.201500559

    Web of Science

    PubMed

  • Liquid-junction-free system for substitutional stripping voltammetry using a closed bipolar electrode system Reviewed

    Shinichiro Takano,Kumi Y. Inoue,Miho Ikegawa,Yasufumi Takahashi,Kosuke Ino,Hitoshi Shiku,Tomokazu Matsue

    ELECTROCHEMISTRY COMMUNICATIONS   66   34 - 37   2016.5( ISSN:1388-2481  eISSN:1873-1902 )

     More details

    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE INC  

    A liquid-junction-free electrochemical system has been developed for substitutional stripping voltammetry (SSV). SSV is a type of stripping analysis that changes the redox current to metal deposition. Generally, SSV requires a liquid junction to maintain electrical conductivity between separated cells. In this report, a closed bipolar electrode system(cBPES) was used to perform SSV without a liquid junction. In this system, electrical conductivity between separated cells was maintained using driving electrodes inserted into each cell. We demonstrated quantification of p-aminophenol (pAP) using a liquid-junction-free system for SSV. We investigated the effect of electrode area ratio on the BPE and the concentration ratio of redox species present in each cell. Then, we calibrated the pAP. A linear relationship between pAP concentration and SSV electrical charge was successfully obtained using our liquid-junction-free system, based on cBPES. This novel analytical system is a promising method for the fabrication of a compact SSV sensor for highly sensitive detections. (C) 2016 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.elecom.2016.02.014

    Web of Science

  • Electrochemical Imaging for Single-cell Analysis of Cell Adhesion Using a Collagen-coated Large-scale Integration (LSI)-based Amperometric Device Reviewed

    Hiroya Abe,Yusuke Kanno,Kosuke Ino,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Hitoshi Shiku,Tomokazu Matsue

    ELECTROCHEMISTRY   84 ( 5 )   364 - 367   2016.5( ISSN:1344-3542 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELECTROCHEMICAL SOC JAPAN  

    We here report the electrochemical imaging of cell adhesion using a large-scale integration (LSI)-based amperometric device, called a Bio-LSI device. The device consists of 400 sensor electrodes arranged with a pitch of 250 pm. The device surface was modified with collagen to assist in the culture of MCF-7 cells and promote their adhesion. The cells disturb the electrochemical reaction of redox mediators, allowing the electrochemical signal to be used to evaluate cell adhesion at the single-cell level. This approach was applied to a cell detachment test. The results show that the Bio-LSI device is a promising tool for single-cell analysis. (C) The Electrochemical Society of Japan, All rights reserved.

    DOI: 10.5796/electrochemistry.84.364

    Web of Science

  • Sequential Monitoring of Oxygen Consumption Rate of Mouse Embryoid Bodies in Glucose-Depleted Solution Reviewed

    Hitoshi Shiku,Nana Aoki,Toshiharu Arai,Yuanshu Zhou,Kumi Y. Inoue,Kosuke Ino,Tomokazu Matsue

    ELECTROCHEMISTRY   84 ( 5 )   302 - 304   2016.5( ISSN:1344-3542 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELECTROCHEMICAL SOC JAPAN  

    Oxygen consumption rate (OCR) of mouse embryoid body (EB) was measured repeatedly in glucose-depleted medium or PBS solution. Non-invasive nature of the scanning electrochemical microscopy allowed sequential OCR measurement of the identical EB sample for more than 24 h. We found that the OCR value for the undifferentiated EB (cultured for 2 days) reached almost zero after 12 h exposure in PBS. On the contrary, the OCR value for the differentiated EB (cultured for 8 days) indicated 40 to 28% normal to the OCR at 0 h-incubation after 24 h exposure in PBS-based solution. It is suggested that differentiated cells indicated a stronger adaptation to glucose deprivation comparing to the undifferentiated cells. (C) The Electrochemical Society of Japan, All rights reserved.

    DOI: 10.5796/electrochemistry.84.302

    Web of Science

  • Potentiometric bioimaging with a large-scale integration (LSI)-based electrochemical device for detection of enzyme activity Reviewed

    Yusuke Kanno,Kosuke Ino,Chika Sakamoto,Kumi Y. Inoue,Masahki Matsudaira,Atsushi Suda,Ryota Kunikata,Tomohiro Ishikawa,Hiroya Abe,Hitoshi Shiku,Tomokazu Matsue

    BIOSENSORS & BIOELECTRONICS   77   709 - 714   2016.3( ISSN:0956-5663  eISSN:1873-4235 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER ADVANCED TECHNOLOGY  

    This paper describes potentiometric bioimaging for enzyme activity using a large-scale integration (LSI)-based electrochemical device with 400 sensors. Potentiometric detection is useful for bioimaging because redox species are not consumed or produced during the detection process; therefore, there is no effect on cell activity and the detectable signal is sustained. In this study, the potentiometer mode of the LSI-based device was applied for the detection of glucose oxidase (GOx) and alkaline phosphatase (ALP) activity. The enzyme activities were quantitatively detected within the concentration ranges of 25-250 mu g/mL and 0.10-5.0 ng/mL. In addition, GOx activity in hydrogels and the ALP activity of embryoid bodies (EBs) from embryonic stem (ES) cells were successfully imaged based on detection of the open circuit potentials of individual sensors in real time. To the best of our knowledge, this is the first report of potentiometric imaging using LSI-based electrochemical arrays to detect enzyme activity in ES cells. The LSI-based device is thus demonstrated to be a promising tool for bioimaging of enzyme activity. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.bios.2015.10.021

    Web of Science

    PubMed

  • Simultaneous Real-Time Monitoring of Oxygen Consumption and Hydrogen Peroxide Production in Cells Using Our Newly Developed Chip-Type Biosensor Device Reviewed

    Ankush Prasad,Hiroyuki Kikuchi,Kumi Y. Inoue,Makoto Suzuki,Yamato Sugiura,Tomoya Sugai,Amano Tomonori,Mika Tada,Masaki Kobayashi,Tomokazu Matsue,Shigenobu Kasai

    Frontiers in Physiology   7   109 - 109   2016.3( ISSN:1664-042X )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    All living organisms bear its defense mechanism. Immune cells during invasion by foreign body undergoes phagocytosis during which monocyte and neutrophil produces reactive oxygen species (ROS). The ROS generated in animal cells are known to be involved in several diseases and ailments, when generated in excess. Therefore, if the ROS generated in cells can be measured and analyzed precisely, it can be employed in immune function evaluation and disease detection. The aim of the current study is to introduce our newly developed chip-type biosensor device with high specificity and sensitivity. It comprises of counter electrode and working electrodes I and II. The counter electrode is a platinum plate while the working electrodes I and II are platinum microelectrode and osmium-horseradish peroxidase modified gold electrode, respectively which acts as oxygen and hydrogen peroxide (H2O2) detection sensors. Simultaneous measurement of oxygen consumption and H2O2 generation were measured in animal cells under the effect of exogenous addition of differentiation inducer, phorbol 12-myristate 13-acetate. The results obtained showed considerable changes in reduction currents in the absence and

    DOI: 10.3389/fphys.2016.00109

    Web of Science

    PubMed

  • DEVELOPMENT OF NOVEL DETECTION SYSTEM USING BIPOLAR ELECTRODE

    Proceedings of the Chemical Sensor Symposium   60   78 - 80   2016.3

     More details

    Language:Japanese  

  • DEVELOPMENT OF BIO-LSI WITH HIGH SPATIAL RESOLUTION FOR ESTIMATION OF CELL RESPIRATORY ACTIVITY

    Proceedings of the Chemical Sensor Symposium   60   63 - 65   2016.3

     More details

    Language:Japanese  

  • Multicolor electrochemical imaging for simultaneous multiplex cell assay using a chip device

    Y. Kanno,K. Ino,H. Abe,K. Y. Inoue,M. Matsudaira,A. Suda,R. Kunikata,H. Shiku,T. Matsue

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   104 - 105   2016.1

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

    In this study, we constructed a new electrochemical imaging system using a chip device for detecting multiple cell activities simultaneously. This system can acquire multicolor electrochemical images composed of electrochemical signals from two different electrodes which were applied with different potentials. We successfully applied the system to measuring respiration activity and dopamine release from a PC12 cell aggregate at the same time. The new imaging system will be a widely applicable analytical method for cell analysis.

  • Multicolor electrochemical imaging for simultaneous multiplex cell assay using a chip device

    Y. Kanno, K. Ino, H. Abe, K. Y. Inoue, M. Matsudaira, A. Suda, R. Kunikata, H. Shiku, T. Matsue

    20th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2016   104 - 105   2016.1(  ISBN:9780979806490 )

     More details

    Language:English  

    In this study, we constructed a new electrochemical imaging system using a chip device for detecting multiple cell activities simultaneously. This system can acquire multicolor electrochemical images composed of electrochemical signals from two different electrodes which were applied with different potentials. We successfully applied the system to measuring respiration activity and dopamine release from a PC12 cell aggregate at the same time. The new imaging system will be a widely applicable analytical method for cell analysis.

    Scopus

  • バイオLSIを用いた光合成活性イメージング

    杉浦 大和, 橋 由佳, 井上 久美, 伊野 浩介, 末永 智一, 葛西 重信

    電気関係学会東北支部連合大会講演論文集   2016   38 - 38   2016

     More details

    Language:Japanese   Publisher:電気関係学会東北支部連合大会実行委員会  

    DOI: 10.11528/tsjc.2016.0_38

  • Amorphous carbon nitride thin films for electrochemical electrode: Effect of molecular structure and substrate materials Reviewed

    Yoshiyuki Kikuchi,Xijiang Chang,Yasuaki Sakakibara,Kumi Y. Inoue,Tomokazu Matsue,Toshihisa Nozawa,Seiji Samukawa

    CARBON   93   207 - 216   2015.11( ISSN:0008-6223  eISSN:1873-3891 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    We report on the effect of molecular structure and substrate material on amorphous carbon nitride (a-CN:H) electrode properties including film adhesion to the substrate and electrochemical properties. Films were prepared by neutral beam enhanced chemical vapor deposition on different substrate materials (p-type Si, Cu, Ti, and Pt) below room temperature. When depositing on Si, doping nitrogen into carbon improved the electrochemical properties despite weak adhesion to the substrate. Nitrogen in a-CN:H formed two different bonding configurations:incorporation into aromatic carbon rings and hydrogen nitride by infrared (IR) spectroscopy. Therefore, delocalization of pi bonds by incorporation of nitrogen affected the electrochemical improvement of the a-CN:H electrode. For samples deposited on a different metal substrate, the adhesion to substrate increased as a function of decreasing oxygen concentration on the metal substrate surface; the Pt substrate performed well with no delamination in our evaluation. The electrochemical properties were improved only in the case of deposition on Pt. Moreover, Pt surface modification by hydrogen beam was also effective; consequently, the electroc

    DOI: 10.1016/j.carbon.2015.05.074

    Web of Science

  • Development of lactate enzyme sensor for subepidermal lactate measurement using enzyme direct patterning

    32   1 - 6   2015.10

     More details

    Language:Japanese   Publisher:Institute of Electrical Engineers of Japan  

  • LSI-based Amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured PC12 cells Reviewed

    H. Abe,K. Ino,C.-Z. Li,Y. Kanno,K. Y. Inoue,A. Suda,R. Kunikata,M. Matsudaira,H. Shiku,T. Matsue

    Proceeding of The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1598 - 1600   2015.10

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

  • A STUDY FOR AMPEROMETRIC MEASUREMENT USING A CLOSED BIPOLAR ELECTRODE SYSTEMS

    Proceedings of the Chemical Sensor Symposium   59   49 - 51   2015.9

     More details

    Language:Japanese  

  • Integration of Boron-Doped Diamond Microelectrode on CMOS-Based Amperometric Sensor Array by Film Transfer Technology Reviewed

    Takeshi Hayasaka,Shinya Yoshida,Kumi Y. Inoue,Masanori Nakano,Tomokazu Matsue,Masayoshi Esashi,Shuji Tanaka

    JOURNAL OF MICROELECTROMECHANICAL SYSTEMS   24 ( 4 )   958 - 967   2015.8( ISSN:1057-7157  eISSN:1941-0158 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC  

    This paper reports on the fabrication of a complementary metal oxide semiconductor (CMOS)-based 20 x 20 amperometric sensor array integrated with boron-doped diamond (BDD) microelectrodes. The BDD electrodes were formed on a Si wafer at 800 degrees C, and then transferred to a 0.18 mu m CMOS large-scale integration (LSI) wafer with a benzocyclobutene bonding interlayer. As a result, the BDD microelectrodes were arrayed without significant damage to CMOS circuit or BDD electrodes. The integrated BDD electrodes on the CMOS LSI exhibited excellent performance for electrochemical analysis. The wider potential window and smaller background current compared with Au microelectrodes were experimentally verified. The electron transfer rate to ferrocenemethanol as a standard reagent was large. The fully implemented device successfully detected 100-nm histamine, and was used for the 2-D real-time imaging of histamine diffused in a solution.

    DOI: 10.1109/JMEMS.2014.2360837

    Web of Science

  • 皮下乳酸モニタリングのための酵素電極センサの開発 Reviewed

    鶴岡典子,井上久美,松永忠雄,末永智一,芳賀洋一

    電気学会研究会資料、センサ・マイクロマシン部門総合研究会   2015.7

     More details

    Language:Japanese   Publishing type:Research paper (scientific journal)  

  • 皮下乳酸モニタリングのための酵素電極センサの開発

    電気学会研究会資料、センサ・マイクロマシン部門総合研究会   PHS-15   51 - 56   2015.7

     More details

    Language:Japanese   Publishing type:Research paper (other science council materials etc.)  

    J-GLOBAL

  • Simulation Analysis of Positional Relationship between Embryoid Bodies and Sensors on an LSI-based Amperometric Device for Electrochemical Imaging of Alkaline Phosphatase Activity Reviewed

    Yusuke Kanno,Kosuke Ino,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Hitoshi Shiku,Tomokazu Matsue

    ANALYTICAL SCIENCES   31 ( 7 )   715 - 719   2015.7( ISSN:0910-6340  eISSN:1348-2246 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:JAPAN SOC ANALYTICAL CHEMISTRY  

    In the present study, we monitored the alkaline phosphatase (ALP) activity of embryoid bodies (EBs) of mouse embryonic stem (ES) cells using a large-scale integration (LSI)-based amperometric device with 400 sensors and a pitch of 250 p.m. In addition, a simulation analysis was performed to reveal the positional relationship between the EBs and the sensor electrodes toward more precise measurements. The study shows that simulation analysis can be applied for precise electrochemical imaging of three-dimensionally cultured cells by normalization of the current signals.

    DOI: 10.2116/analsci.31.715

    Web of Science

    PubMed

  • Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors Reviewed

    Hiroya Abe,Kosuke Ino,Chen-Zhong Li,Yusuke Kanno,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Yasufumi Takahashi,Hitoshi Shiku,Tomokazu Matsue

    ANALYTICAL CHEMISTRY   87 ( 12 )   6364 - 6370   2015.6( ISSN:0003-2700 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER CHEMICAL SOC  

    In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 mu m for rapid electroehemical imaging of large, areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopai-nine release fibril the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bia-LSI clearly showed the effects of the dopaminergic drugs L-3,4-dihydroxyphenylalanine (L-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Out results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.

    DOI: 10.1021/acs.analchem.5b01307

  • Electrochemical Imaging of Dopamine Release from Three-Dimensional-Cultured PC12 Cells Using Large-Scale Integration-Based Amperometric Sensors Reviewed

    Hiroya Abe,Kosuke Ino,Chen-Zhong Li,Yusuke Kanno,Kumi Y. Inoue,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Yasufumi Takahashi,Hitoshi Shiku,Tomokazu Matsue

    ANALYTICAL CHEMISTRY   87 ( 12 )   6364 - 6370   2015.6( ISSN:0003-2700 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER CHEMICAL SOC  

    In the present study, we used a large-scale integration (LSI)-based amperometric sensor array system, designated Bio-LSI, to image dopamine release from three-dimensional (3D)-cultured PC12 cells (PC12 spheroids). The Bio-LSI device consists of 400 sensor electrodes with a pitch of 250 mu m for rapid electroehemical imaging of large, areas. PC12 spheroids were stimulated with K+ to release dopamine. Poststimulation dopai-nine release fibril the PC12 spheroids was electrochemically imaged using the Bio-LSI device. Bia-LSI clearly showed the effects of the dopaminergic drugs L-3,4-dihydroxyphenylalanine (L-DOPA) and reserpine on K+-stimulated dopamine release from PC12 spheroids. Out results demonstrate that dopamine release from PC12 spheroids can be monitored using the device, suggesting that the Bio-LSI is a promising tool for use in evaluating 3D-cultured dopaminergic cells and the effects of dopaminergic drugs. To the best of our knowledge, this report is the first to describe electrochemical imaging of dopamine release by PC12 spheroids using LSI-based amperometric sensors.

    DOI: 10.1021/acs.analchem.5b01307

  • CMOS型電気化学デバイスを用いた神経細胞塊から放出される神経伝達物質のイメージング

    阿部博弥,伊野浩介,LI Chen‐Zhong,菅野祐介,井上久美,國方亮太,須田篤史,松平昌昭,高橋康史,珠玖仁,末永智一

    電気化学会大会講演要旨集(CD-ROM)   82nd   ROMBUNNO.PS31   2015.3

     More details

    Language:Japanese   Publishing type:Research paper (research society, symposium materials, etc.)  

    J-GLOBAL

  • Feedback mode-based electrochemical imaging of conductivity and topography for large substrate surfaces using an LSI-based amperometric chip device with 400 sensors Reviewed

    Yusuke Kanno,Kosuke Ino,Kumi Y. Inoue,Mustafa Sen,Atsushi Suda,Ryota Kunikata,Masahki Matsudaira,Hiroya Abe,Chen-Zhong Li,Hitoshi Shiku,Tomokazu Matsue

    JOURNAL OF ELECTROANALYTICAL CHEMISTRY   741   109 - 113   2015.3( ISSN:1572-6657  eISSN:1873-2569 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE SA  

    Feedback mode-based electrochemical imaging of conductivity and topography for large substrate surfaces is presented using a large-scale integration (LSI)-based amperometric chip device with 400 sensors at a pitch of 250 lam. The LSI-based chip device has enabled rapid electrochemical imaging of large substrate surfaces, compared to scanning electrochemical microscope (SECM). Substrates modified with conductive and insulating materials were placed onto the device to acquire electrochemical signals from the substrate surface using positive and negative feedback signals. The conductivity and topography of the substrate were successfully imaged, indicating that the feedback mode-based electrochemical imaging with such a device is useful to characterize large-area substrate surfaces. (C) 2015 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.jelechem.2015.01.020

    Web of Science

  • CHARACTERIZATION OF NOVEL SUBSTRATE FOR CASPASE-3 AND ITS APPLICATION FOR A SIMPLE APOPTOSIS SENSOR

    Proceedings of the Chemical Sensor Symposium   58   127 - 129   2015.3

     More details

    Language:Japanese  

  • Potentiometric imaging of stem cells using an lsi-based electrochemical chip device with 400 microelectrodes

    Y. Kanno,K. Ino,C. Sakamoto,K. Y. Inoue,M. Matsudaira,A. Suda,R. Kunikata,H. Abe,H. Shiku,T. Matsue

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1686 - 1688   2015.1

     More details

    Language:English   Publishing type:Research paper (international conference proceedings)  

    © 15CBMS-0001. In this study, we demonstrated a potentiometric imaging of enzyme activity in mouse embryonic stem (ES) cells using an LSI-based electrochemical chip device with 400 microelectrodes. Successful results on the detection of the alkaline phosphatase activity of ES cells show that the potentiometric imaging using our LSI-based device will be a widely applicable method for cell analysis.

  • Potentiometric imaging of stem cells using an lsi-based electrochemical chip device with 400 microelectrodes

    Y. Kanno, K. Ino, C. Sakamoto, K. Y. Inoue, M. Matsudaira, A. Suda, R. Kunikata, H. Abe, H. Shiku, T. Matsue

    MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1686 - 1688   2015.1(  ISBN:9780979806483 )

     More details

    Language:English  

    © 15CBMS-0001. In this study, we demonstrated a potentiometric imaging of enzyme activity in mouse embryonic stem (ES) cells using an LSI-based electrochemical chip device with 400 microelectrodes. Successful results on the detection of the alkaline phosphatase activity of ES cells show that the potentiometric imaging using our LSI-based device will be a widely applicable method for cell analysis.

    Scopus

  • LSI-based Amperometric device for electrochemical imaging of drug effect on dopamine release from three-dimensional cultured PC12 cells Reviewed

    H. Abe, K. Ino, C.-Z. Li, Y. Kanno, K. Y. Inoue, A. Suda, R. Kunikata, M. Matsudaira, H. Shiku, T. Matsue

    Proceeding of The 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences   1598 - 1600   2015(  ISBN:9780979806483 )

     More details

    Language:English  

    We report a large-scale integration (LSI) based amperometric chip device containing 400 sensors with a pitch of 250 μm and its applications for biological analysis of dopamine release from three-dimensional (3D) cultured cells using rat pheochromocytoma (PC12) cells as a neural cell model. The 3D cultured PC12 cells (PC12 spheroids) were stimulated with a high K+ solution to induce dopamine release. We showed clearly drug effects of dopaminergic drugs L-3,4-dihydroxyphenylalanine, (L-DOPA) and reserpine on dopamine release from PC12 spheroids. Our results indicate that the device is a useful tool for evaluating the effects of dopaminergic drugs.

    Scopus

  • Advanced LSI-based amperometric sensor array with light-shielding structure for effective removal of photocurrent and mode selectable function for individual operation of 400 electrodes Reviewed

    Kumi Y. Inoue,Masahki Matsudaira,Masanori Nakano,Kosuke Ino,Chika Sakamoto,Yusuke Kanno,Reyushi Kubo,Ryota Kunikata,Atsushi Kira,Atsushi Suda,Ryota Tsurumi,Toshihito Shioya,Shinya Yoshida,Masanori Muroyama,Tomohiro Ishikawa,Hitoshi Shiku,Shiro Satoh,Masayoshi Esashi,Tomokazu Matsue

    LAB ON A CHIP   15 ( 3 )   848 - 856   2014.11( ISSN:1473-0197  eISSN:1473-0189 )

     More details

    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    We have developed a large-scale integrated (LSI) complementary metal-oxide semiconductor (CMOS)-based amperometric sensor array system called "Bio-LSI" as a platform for electrochemical bio-imaging and multi-point biosensing with 400 measurement points. In this study, we newly developed a Bio-LSI chip with a light-shield structure and a mode-selectable function with the aim of extending the application range of Bio-LSI. The light shield created by the top metal layer of the LSI chip significantly reduces the noise generated by the photocurrent, whose value is less than 1% of the previous Bio-LSI without the light shield. The mode-selectable function enables the individual operation of 400 electrodes in off, electrometer, V1, and V2 mode. The off-mode cuts the electrode from the electric circuit. The electrometer-mode reads out the electrode potential. The V1-mode and the V2-mode set the selected sensor electrode at two different independent voltages and read out the current. We demonstrated the usefulness of the mode-selectable function. First, we displayed a dot picture based on the redox reactions of 2.0 mM ferrocenemethanol at 400 electrodes by applying two different independent

    DOI: 10.1039/c4lc01099j

    Web of Science

    PubMed

  • Conductive Diamond-like Carbon Film Deposition by Low Temperature Neutral Beam Enhanced Chemical Vapor Deposition for Bio-LSIs Reviewed

    Xijian Chang, Yoshiyuki Kikuchi, Tomohiro Kubota, Kumi Y. Inoue, Tomokazu Matsue, Seiji Samukawa

    2014 International Conference on Solid State Devices and Materials   2014.9

     More details

    Language:English  

  • DEVELOPMENT OF ELECTROCHEMICAL CASPASE-3 DETECTION METHODS AND THEIR APPLICATION FOR CELL APOPTOSIS ASSAY

    Proceedings of the Chemical Sensor Symposium   57   88 - 90   2014.9

     More details

    Language:Japanese  

  • Improvement of LSI-based amperometric sensor array for wide application of bioimaging and biosensing Reviewed

    Kumi Y. Inoue, Masahki Matsudaira, Kosuke Ino, Masanori Nakano, Kosuke Takara, Atsushi Suda, Ryota Kunikata, Shinya Yoshida, Takeshi Hayasaka, Yoshiyuki Kikuchi, Xijiang Chang, Tomohiro Kubota, Hitoshi Shiku, Shuji Tanaka, Seiji Samukawa, Tomokaz

    65th Annual Meeting of the International Society of Electrochemistry   2014.9

     More details

    Authorship:Lead author   Language:English  

  • Conductive DLC deposition by NBECVD for application of Bio-LSI Reviewed

    Xijiang Chang, Yoshiyuki Kikuchi, Tomohiro Kubota, Kumi Y.Inoue, Tomokazu Matsue, Seiji Samukawa

    The 6th IEEE International Nanoelectronics Conference 2014   2014.7

     More details

    Language:English  

  • ダイヤモンド集積化LSIによるバイオセンシング・イメージング

    吉田慎哉, 井上久美, 末永智一

    InterLab   ( 110 )   24 - 29   2014.3

     More details

    Language:Japanese   Publishing type:(MISC) Introduction and explanation (commerce magazine)   Publisher:株式会社オプトロニクス社  

  • DEVELOPMENT OF LIQUID-JUNCTION-FREE SUBSTITUTIONAL STRIPPING DEVICE FOR HIGHLY SENSITIVE BIOSENSING

    Proceedings of the Chemical Sensor Symposium   56   10 - 12   2014.3

     More details

    Language:Japanese  

  • Diamond on CMOS for Amperometric Bio-imaging

    Shinya Yoshida, Takeshi Hayasaka, Kumi Y. Inoue, Masanori Nakano, Shuji Tanaka, Tomokazu Matsue, Masayoshi Esashi

    Abstracts of The AIMR International Symposium 2014 (AMIS 2014)   2014   88 - 88   2014

     More details

    Language:English  

  • バイオLSIを利用した多項目多サンプル同時免疫計測システムの開発

    北東俊輝, 安川智之, 國方亮太, 須田篤史, 井上(安田)久美, 井上(安田)久美, 末永智一, 末永智一, 末永智一, 水谷文雄

    分析化学討論会講演要旨集   74th   2014

     More details

    Language:Japanese  

    J-GLOBAL

  • [title in Japanese]

    Kumi Y. INOUE

    Electrochemistry   82 ( 3 )   202 - 203   2014( ISSN:1344-3542  eISSN:2186-2451 )

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (others)   Publisher:The Electrochemical Society of Japan  

    DOI: 10.5796/electrochemistry.82.202

  • [title in Japanese]

    Kumi Y. INOUE

    Electrochemistry   82 ( 3 )   202 - 203   2014( ISSN:1344-3542  eISSN:2186-2451 )

     More details

    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:(MISC) Introduction and explanation (others)   Publisher:The Electrochemical Society of Japan  

    DOI: 10.5796/electrochemistry.82.202

  • Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device Reviewed

    Kosuke Ino,Takehito Goto,Yusuke Kanno,Kumi Y. Inoue,Yasufumi Takahashi,Hitoshi Shiku,Tomokazu Matsue

    LAB ON A CHIP   14 ( 4 )   787 - 794   2013.11( ISSN:1473-0197  eISSN:1473-0189 )

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ROYAL SOC CHEMISTRY  

    We have previously reported a local redox cycling-based electrochemical (LRC-EC) system for the incorporation of many electrochemical sensors into a small chip device. In the present study, a new type of LRC-EC chip device was fabricated for the detection of a droplet array. To detect electrochemically redox compounds in droplets, Pt pseudo-reference/counter electrodes were incorporated into the individual sensors of the LRC-EC chip device. Cyclic voltammetry for the LRC-EC chip device with internal Pt pseudo-reference electrodes indicated well-defined voltammograms based on redox cycling for the individual sensor points. The device was successfully applied to the addressable detection of alkaline phosphatase (ALP) activity of HeLa cells in single droplets on the sensor points. Therefore, the LRC-EC chip device is considered to be a useful device for the bioanalysis of droplet systems.

    DOI: 10.1039/c3lc51156a

    Web of Science

    PubMed

  • Fabrication of Conductive Diamond Electrode on LSI-Integrated Amperometric Sensor Array

    30   1 - 5   2013.11

     More details

    Language:Japanese   Publisher:Institute of Electrical Engineers of Japan  

  • LSI-BASED AMPEROMETRIC SENSOR FOR REAL-TIME MONITORING OF THREE-DIMENSIONAL CULTURE CELL

    Proceedings of the Chemical Sensor Symposium   54   117 - 119   2013.3

     More details

    Language:Japanese  

  • LSI集積化アンペロメトリックセンサアレイへの導電性ダイヤモンド電極の形成 Reviewed

    早坂 丈, 吉田 慎哉, 井上久美, 末永 智一, 江刺 正喜, 田中 秀治

    第5回「集積化MEMSシンポジウム」   5   6PM2-E-1 - 6PM2-E-1   2013

     More details

    Language:Japanese  

  • 安価な簡易型センサの実用化に向けた電気化学エンドトキシン検出法の開発 Invited

    井上(安田)久美, 髙野 真一朗, 高橋 里子, 石田 洋祐, 伊藤(佐々木, 隆広, 伊野 浩介, 珠玖 仁, 末永 智一

    エンドトキシン・自然免疫研究   16   7 - 11   2013

     More details

    Language:Japanese   Publishing type:(MISC) Introduction and explanation (others)  

  • Development of Chip-type Electrochemical Biosensors for Clinical Use Invited

    Kumi Y. Inoue

    Chemical Sensors   28 ( 3 )   114 - 121   2012.9

     More details

    Language:Japanese   Publishing type:(MISC) Introduction and explanation (others)  

    CiNii Books

  • DEVELOPMENT OF HIGHLY SENSITIVE ELECTROCHEMICAL ENDOTOXIN DETECTION METHOD USING LIMULUS AMEBOCYTE LYSATE ASSAY TECHNIQUE

    Proceedings of the Chemical Sensor Symposium   53   31 - 33   2012.3

     More details

    Language:Japanese  

  • Development of highly sensitive endotoxin sensor using limulus amebocyte lysate assay technique

    Proceedings of the Chemical Sensor Symposium   51   52 - 54   2011.3

     More details

    Language:Japanese  

  • Electrochemical detection for endotoxin using recombinant limulus factor C

    Proceedings of the Chemical Sensor Symposium   49   136 - 138   2010.3

     More details

    Language:Japanese  

  • Cell-based biosensor for detecting toxic activities using leucocytes

    Proceedings of the Chemical Sensor Symposium   47   151 - 153   2009.3

     More details

    Language:Japanese  

  • 細胞機能を利用するマイクロバイオセンサ Invited

    井上(安田)久美, 珠玖 仁, 末永智一

    マテリアルインテグレーション   第21 ( 第05、06 )   232 - 241   2008.5( ISSN:1344-7858 )

     More details

    Language:Japanese   Publishing type:(MISC) Introduction and explanation (scientific journal)   Publisher:株式会社ティー・アイ・シィー  

    CiNii Books

▼display all

Books and Other Publications

  • 近未来のデジタルヘルスを支える酵素バイオ技術 : センサと発電

    冨永, 昌人( Role: Contributorバイポーラ電極によるバイオセンシング・イメージング)

    シーエムシー出版  2022.2   ISBN:9784781316604

     More details

    Total pages:xii, 410p   Language:Japanese  

    CiNii Books

  • バイオセンサの迅速・簡易・高機能化技術と課題解決書(仮題)

    井上(安田)久美, 珠玖 仁, 末永智一( Role: Joint Work第3部バイオマーカーをバイオセンサに応用させるための基礎知識 第2章バイオセンサで検出可能にするための課題 第2節電気化学エンドトキシンセンサのための高感度検出法)

    株式会社技術情報協会  2013 

     More details

    Language:Japanese   Book type:Scholarly book

  • 先進化学センサ

    井上(安田)久美, 珠玖 仁, 末永智一( Role: Joint Work第2章細胞センシング・マイクロシステム第1節細胞機能を利用するマイクロバイオセンサ243ページから252ページ)

    株式会社ティー・アイ・シィー出版  2008 

     More details

    Language:Japanese   Book type:Scholarly book

Presentations

  • Fabrication of Vertical Electrode Array using Carbon Paste for Bipolar Electrochemical Microscopy International conference

    Kumi. Y. Inoue, Tomoki Iwama, Koki Kubota, Ayane Endo, Takahiro-Ito Sasaki, Yuri Totsuka, Shion Mizukura, and Hitoshi Shiku

    14th Asian Conference on Chemical Sensors (ACCS 2023)  2023.11 

     More details

    Event date: 2023.11

    Language:English   Presentation type:Oral presentation(general)  

    Venue:University of Santo Tomas, Manila, Philippines  

  • クローズドバイポーラ電気化学顕微鏡の実現に向けた多孔質膜への導電性ペースト充填の検討

    井上(安田) 久美、遠藤 彩音、久保田 恒喜、 遠藤 彩音、伊藤 隆広、戸塚 友理

    電気化学会 2023電気化学秋季大会  2023.9 

     More details

    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:九州大学伊都キャンパス  

  • Bipolar electrode array for dopamine imaging using cathodic luminophore for electrochemiluminescence International conference

    Kumi Y. Inoue, Ayane Endo, Koki Kubota, Mayo Komatsu, Tomoki Iwama, Hitoshi Shiku

    74th Annual Meeting of the International Society of Electrochemistry  2023.9 

     More details

    Event date: 2023.9

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Centre de Congrès de Lyon, France  

  • プロテアーゼ反応を用いる高感度・小型・簡便な電気化学センサ Invited

    井上(安田) 久美

    第24 回酵素応用シンポジウム  2023.6 

     More details

    Event date: 2023.6

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:北名古屋市 天野エンザイム㈱ 慈善堂ホール  

  • Analysis of electrochemiluminescence behavior by potential difference measurement between closed bipolar electrode and solutions

    Kumi Y. Inoue, Takaaki Abe, Koki Kubota, Ayane Endo, Yuri Totsuka

    2023.3 

     More details

    Event date: 2023.3

    Language:Japanese  

  • Analysis of electrochemiluminescence behavior by potential difference measurement between closed bipolar electrode and solutions

    Kumi Y. Inoue, Takaaki Abe, Koki Kubota, Ayane Endo, Yuri Totsuka

    2023.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 電気化学分析-バイオセンサ・バイオイメージングへの応用 Invited

    井上 久美

    第2回梨大医工融合セミナー  2023.3 

     More details

    Event date: 2023.3

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

    Venue:シミックプラザ  

  • 電気化学分析-バイオセンサ・バイオイメージングへの応用-

    井上 久美

    山梨大学医工融合セミナー  2023.3 

     More details

    Language:Japanese   Presentation type:Symposium workshop panel(nominated)  

  • バイポーラ電気化学顕微鏡による生命システムの計測

    井上 久美

    創発研究者とマテリアル先端リサーチインフラの出会いの場  2023.2 

     More details

    Event date: 2023.2

    Language:Japanese   Presentation type:Poster presentation  

    Venue:⼤阪⼤学産業科学研究所  

  • Electrochemical Biosensors and Biosensing Invited

    Kumi Y. Inoue

    2023.1 

     More details

    Event date: 2023.1

    Language:Japanese   Presentation type:Poster presentation  

  • Electrochemical Biosensors and Biosensing

    2023.1 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  • Fabrication of a high density closed bipolar electrode array for an imaging element of bipolar electrochemical microscopy Invited

    Kumi. Y. Inoue, Koki Kubota, Tomoki Iwama, Yuri Totsuka, Ayane Endo, Tetsuo Kuwabara

    2022.12 

     More details

    Event date: 2022.12

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • Fabrication of a high density closed bipolar electrode array for an imaging element of bipolar electrochemical microscopy Invited

    Kumi. Y. Inoue, Koki Kubota, Tomoki Iwama, Yuri Totsuka, Ayane Endo, Tetsuo Kuwabara

    2022.12 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • バイオセンサ・バイオイメージング

    井上 久美, 久保田 恒喜

    山梨テクノICTメッセ2022  2022.11 

     More details

    Event date: 2022.11

    Language:Japanese   Presentation type:Other  

  • バイポーラ電気化学顕微鏡で用いる発光基質の酸化還元電位と電気化学発光の関係

    阿部 岳晃 , 岩間 智紀 , 小松 万葉 , 久保田 恒喜 , 遠藤 彩音 , 戸塚 友理 , 珠玖 仁 , 井上(安田)久美

    第68回ポーラログラフィーおよび電気分析化学討論会  2022.11 

     More details

    Event date: 2022.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都大学 桂キャンパス 桂ホール(Bクラスター 事務管理棟1階)  

  • 導電性ペーストに分散させたカソ―ディックルミノフォアからの電気化学発光の高輝度化

    久保田 恒喜, 遠藤 彩音, 小松 万葉, 岩間 智紀,井上(安田)久美

    第68回ポーラログラフィーおよび電気分析化学討論会  2022.11 

     More details

    Event date: 2022.11

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都大学 桂キャンパス 桂ホール(Bクラスター 事務管理棟1階)  

  • バイポーラ電気化学顕微鏡による生命システムの計測

    井上 久美

    2022年度北川パネル進捗報告会  2022.10 

     More details

    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:オンライン  

  • Bipolar electrochemical microscopy for real-time chemical imaging using nanocarbon paste microelectrode array International conference

    Kumi. Y. Inoue, Tomoki Iwama, Koki Kubota, Ryusei Naganuma, Hitoshi Shiku

    73rd Annual Meeting of the International Society of Electrochemistry  2022.9 

     More details

    Event date: 2022.9

    Language:English   Presentation type:Oral presentation(general)  

    Venue:online  

  • Bipolar electrochemical microscopy for real-time chemical imaging using nanocarbon paste microelectrode array

    Kumi. Y. Inoue, Tomoki Iwama, Koki Kubota, Ryusei Naganuma, Hitoshi Shiku

    73rd Annual Meeting of the International Society of Electrochemistry  2022.9 

     More details

    Language:English  

  • Proposal of conductive paste packing method to arrayed micro through-holes

    Koki Kubota, Yuuri Totsuka, Ayane Endo, Ryusei Naganuma, Tomoki Iwama, Tetsuo Kuwabara, Kumi Y. Inoue

    2022.9 

     More details

    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Surface modification of pH-sensitive ISFET to use as aptasensor

    Kumi Y. Inoue, Siti Masturah binti Fakhruddin, Masayoshi Esashi, and Hitoshi Shiku.

    2022.9 

     More details

    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Surface modification of pH-sensitive ISFET to use as aptasensor.

    Kumi Y. Inoue, Siti Masturah, binti Fakhruddin, Masayoshi Esashi, Hitoshi Shiku

    2022.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

  • バイポーラ電気化学顕微鏡による生命システムの計測

    井上 久美

    創発の場ーフォトニクス討論会ーin 徳島  2022.9 

     More details

    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:徳島⼤学ポストLEDフォトニクス研究所  

  • バイポーラ電気化学顕微鏡による生命システムの計測

    井上(安田)久美

    創発の場 -フォトニクス討論会- in 徳島  2022.9 

     More details

    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

  • バイポーラ電気化学顕微鏡による生命システムの計測

    井上 久美

    JST創発的研究支援事業「融合の場」第1回公開シンポジウム  2022.6 

     More details

    Event date: 2022.6

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:東京工業大学 ディジタル多目的ホール(大岡山キャンパス西9号館)  

  • ナノバイポーラ電極アレによる細胞間伝達物質の可視化向けた検討

    井上 久美

    電気学会 光・量子デバイス技術委員会「量子ビームによるナノ構造・界面形成とバイオメディカル応用技術」調査専門委員会 第5回委員会  2022.5 

     More details

    Event date: 2022.5

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

    Venue:オンライン  

  • カソーディックルミノフォアを用いるバイポーラ電気化学顕微鏡によるドーパミン検出

    井上(安田) 久美、小松 万葉、岩間 智紀、珠玖 仁

    電気化学会第89回大会  2022.3  電気化学会

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:大阪府立大学  

  • Dopamine detection with bipolar electrochemical microscopy using cathodic luminophore

    Kumi Y. Inoue, Mayo Komatsu, Tomoki Iwama, Hitoshi Shiku

    2022.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

  • バイポーラ電気化学顕微鏡による生命システムの計測

    井上 久美

    創発的研究支援事業「北川パネル」第2回創発の場  2022.3 

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:Online  

  • 生物工学-バイオセンサをつくろう-

    井上 久美

    令和3年度「女子中高生と保護者のための理工系Web講座」  2022.3 

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • 生物工学:バイオセンサをつくろう

    井上 久美

    令和3年度女子中高生の理系進路選択支援プログラム 理工系Web講座  2022.3 

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • ヘルスケアを志向する電気化学バイオセンサおよびバイオイメージングデバイスの開発

    井上(安田)久美

    第 4 回 COI 学術交流会  2022.2 

     More details

    Event date: 2022.2

    Language:Japanese   Presentation type:Poster presentation  

  • ヘルスケアを志向する電気化学バイオセンサおよびバイオイメージングデバイスの開発

    井上(安田)久美

    第 4 回 COI 学術交流会  2022.2 

     More details

    Language:Japanese   Presentation type:Poster presentation  

  • デジタルヘルスのためのセンサ開発と細胞間物質移動をイメージングする顕微鏡への展開

    井上(安田)久美

    第28回アカデミックサロン  2022.1 

     More details

    Event date: 2022.1

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • デジタルヘルスのためのセンサ開発と細胞間物質移動をイメージングする顕微鏡への展開

    井上 久美

    第28回アカデミックサロン  2022.1 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • ルテニウムビピリジン錯体と酸化型グルタチオンを陰極発光ルミノフォアとして用いるバイポーラ電気化学顕微鏡システムによるドーパミンイメージング Invited

    井上(安田)久美、小松万葉、岩間智紀、珠玖仁

    第31回日本MRS年次大会  2021.12  日本MRS

     More details

    Event date: 2021.12

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:パシフィコ横浜ノース G214  

  • ルテニウムビピリジン錯体と酸化型グルタチオンを陰極発光ルミノフォアとして用いるバイポーラ電気化学顕微鏡システムによるドーパミンイメージング Invited

    井上(安田)久美, 小松万葉, 岩間智紀, 珠玖仁

    第31回日本MRS年次大会  2021.12 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • 電気化学センサによるバイオセンシング・バイオイメージング Invited

    井上(安田)久美

    日本化学会関東支部講演会  2021.12 

     More details

    Event date: 2021.12

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • 電気化学センサによるバイオセンシング・バイオイメージング Invited

    井上 久美

    日本化学会関東支部講演会  2021.12 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • Dopamine imaging using cathodic electrochemiluminescence generated by co-reaction of ruthenium bipyridine complex and oxidized glutathione on closed bipolar electrode array International conference

    Kumi. Y. Inoue, Mayo Komatsu, Tomoki Iwama, Tomokazu Matsue, Hitoshi Shiku

    72nd Annual ISE Meeting  2021.9 

     More details

    Event date: 2021.9

    Language:English   Presentation type:Oral presentation(general)  

  • Dopamine imaging using cathodic electrochemiluminescence generated by co-reaction of ruthenium bipyridine complex and oxidized glutathione on closed bipolar electrode array

    Kumi. Y. Inoue, Mayo Komatsu, Tomoki Iwama, Tomokazu Matsue, Hitoshi Shiku

    72nd Annual ISE Meeting  2021.9 

     More details

    Language:English   Presentation type:Oral presentation(general)  

  • 超高感度電流検出型CMOSバイオイメージセンサバイオLSI Invited

    井上 久美

    第33回マルチモーダルバイオイメージセンサ研究会  2021.6 

     More details

    Event date: 2021.6

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • クローズドバイポーラ電極を用いるバイオセンシング・バイオイメージングデバイスの開発 Invited

    井上(安田) 久美, シティ マストゥラ, 岩間 智紀, 珠玖 仁, 末永 智一

    第30回日本MRS年次大会  2020.12 

     More details

    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:WEB開催  

  • クローズドバイポーラ電極を用いるバイオセンシング・バイオイメージングデバイスの開発 Invited

    井上(安田) 久美, シティ マストゥラ, 岩間 智紀, 珠玖 仁, 末永 智一

    第30回日本MRS年次大会  2020.12 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • バイポーラ電極アレイを利用する電気化学イメージングの原理検証

    井上(安田)久美、岩間智紀、小松万葉、末永智一、珠玖仁

    第66回ポーラログラフィーおよび電気分析化学討論会  2020.11 

     More details

    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:WEB開催  

  • バイポーラ電極アレイを利用する電気化学イメージングの原理検証

    第66回ポーラログラフィーおよび電気分析化学討論会  2020.11 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Closed bipolar electrochemistry for sensor and bioimaging applications Invited International conference

    K. Y. Inoue, T. Iwama, Siti Masturah Fakhruddin, H. Shiku and T. Matsue

    2020.10 

     More details

    Event date: 2020.10

    Language:English   Presentation type:Oral presentation(invited, special)  

    Venue:Digital Meeting  

  • Closed bipolar electrochemistry for sensor and bioimaging applications Invited

    K. Y. Inoue, T. Iwama, Siti Masturah, Fakhruddin, H. Shiku, T. Matsue

    PRiMe 2020  2020.10 

     More details

    Event date: 2020.10 - 2020.11

    Language:English   Presentation type:Oral presentation(invited, special)  

  • バイオセンサーを用いた日常人間ドック Invited

    井上(安田) 久美

    第20回日本抗加齢医学会総会  2020.9 

     More details

    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:WEB開催・一部浜松町コンベンションホール開催  

  • バイオセンサーを用いた日常人間ドック Invited

    井上(安田) 久美

    第20回日本抗加齢医学会総会  2020.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • Electrochemical biosensors for easy and highly sensitive monitoring of biomolecules

    2020.7 

     More details

    Event date: 2020.7

    Language:Japanese   Presentation type:Poster presentation  

  • Electrochemical biosensors for easy and highly sensitive monitoring of biomolecules

    2020.7 

     More details

    Language:Japanese  

  • Chemical imaging system using closed bipolar electrode array Invited International conference

    K. Y. Inoue, T. Iwama, H. Shiku and T. Matsue

    The 13th Asian Conference on Chemical Sensors (ACCS2019)  2019.11 

     More details

    Event date: 2019.11

    Language:English   Presentation type:Oral presentation(invited, special)  

    Venue:Grand Inna Sanur Bali, Indonesia  

  • Chemical imaging system using closed bipolar electrode array Invited

    K. Y. Inoue, T. Iwama, H. Shiku, T. Matsue

    The 13th Asian Conference on Chemical Sensors (ACCS2019)  2019.11 

     More details

    Language:English   Presentation type:Oral presentation(invited, special)  

  • Analytical applications of closed bipolar electrode array and consideration of its operating principle International conference

    Kumi Y. Inoue, Siti Masturah Fakhruddin, Tomoki Iwama, Miho Ikegawa, Hitoshi Shiku, Tomokazu Matsue.

    The International Joint Meeting of the Polarographic Society of Japan (PSJ) and National Taiwan University (NTU)  2019.11 

     More details

    Event date: 2019.11

    Language:English   Presentation type:Poster presentation  

    Venue:National Taiwan University, Taipei, Taiwan  

  • Analytical applications of closed bipolar electrode array and consideration of its operating principle

    Kumi Y. Inoue, Siti Masturah, Fakhruddin, Tomoki Iwama, Miho Ikegawa, Hitoshi Shiku, Tomokazu Matsue

    The International Joint Meeting of the Polarographic Society of Japan (PSJ) and National Taiwan University (NTU)  2019.11 

     More details

    Language:English   Presentation type:Poster presentation  

  • Electrochemical urine sensor using proteases

    Kumi Y. Inoue, Tsubasa Miura, Tomokazu Matsue

    2019.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 試薬添加不要の尿タンパクセンサの開発 Invited

    井上(安田) 久美, 三浦 翼, 末永 智一

    第28回日本MRS年次大会  2018.12 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • (志方メダル受賞講演)簡便に高感度定量分析を達成する電気化学センサの研究 Invited

    INOUE Kumi Y

    第64回ポーラログラフィーおよび電気分析化学討論会  2018.11 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:壱岐の島ホール  

  • (清山賞受賞講演)医療・バイオ分野での利用に資するアンペロメトリックセンシングチップの開発 Invited

    INOUE Kumi Y

    2018年電気化学会秋季大会・第64回化学センサ研究発表会  2018.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:金沢大学 角間キャンパス  

  • Liquid-junction-free Amperometric Sensing System Based on Closed Bipolar Electrode System International conference

    Kumi Y. Inoue, Shinichiro Takano, Miho Ikegawa, Ryoto Tsuga, Siti Masturah, binti Fakhruddin, Hitoshi Shiku, Tomokazu Matsue

    69th Annual Meeting of the International Society of Electrochemistry  2018.9 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Nuovo Polo Congressuale Bologna, Italy  

  • 大学ってどんなところ ~ 実験が知識蓄積の虚無感から解放してくれた ~ Invited

    INOUE Kumi Y

    平成30年度東北大学工学部オープンキャンパス、女子学生のためのミニフォーラム「工学にかける私の夢」  2018.7 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • Liquid-junction Free Reference Electrode Integration into Amperometric Sensor Probe Using Closed Bipolar Electrode System International conference

    Kumi Y. Inoue, Ryoto Tsuga, Siti Masturah, binti Fakhruddin, Tomokazu Matsue

    17th International Meeting on Chemical Sensors  2018.7 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:University of Vienna, Austria  

  • 簡易なバイオ計測のための電気化学デバイス Invited

    井上, 安田) 久美

    東日本分析化学若手交流会  2018.7 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:松島新富亭  

  • 電気化学エンドトキシンセンサ技術をベースとするエンドトキシン測定装置事業立上げのための事業化企画およびプロトタイプ製作による事業化検証 Invited

    INOUE Kumi Y

    事業イノベーションセンターマッチングイベント  2018.6 

     More details

    Language:Japanese   Presentation type:Other  

  • Electrochemical Approach to Protease Activity Assay Invited International conference

    Kumi Y. Inoue

    2nd International Symposium on Chemical Communication  2018.5 

     More details

    Language:English   Presentation type:Oral presentation(invited, special)  

    Venue:ohoku University, Japan  

  • Close型バイポーラ電極による液絡不要の参照電極集積型アンペロメトリックセンシングプローブの開発

    井上(安田)久美, シティ マストゥラ, 都賀 亮人, 伊藤, 佐々木 隆広, 珠玖 仁, 末永 智一

    電気化学会第85回大会(第63回化学センサ研究発表会)  2018.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:東京理科大学葛飾キャンパス  

  • Close型バイポーラ電極システムを用いる参照電極の液絡を必要としない多点アンペロメトリック検出法の開発

    井上(安田)久美, 池川未歩, 伊藤-佐々木隆広, 伊野浩介, 珠玖仁, 末永智一

    第63回ポーラログラフィーおよび電気分析化学討論会  2017.11 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:海峡メッセ下関  

  • Liquid-junction-free reference electrode system for amperometry using a closed bipolar electrode International conference

    Kumi Y. Inoue, Miho Ikegawa, Takahiro Ito-Sasaki, Shinichiro Takano, Hitoshi Shiku, Tomokazu Matsue

    The 12th Asian Conference on Chemical Sensors (ACCS2017)  2017.11 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Pan Pacific Hanoi, Hanoi, Vietnam  

  • Electrochemical Approach for Protease Sensing Invited International conference

    Kumi Y. Inoue, Sixiang Sun, Shinichiro Takano, Shusaku Shiomoto, Takahiro Ito-Sasaki, Tomokazu Matsue

    The International Workshop: New Electroanalytical Techniques and Their Emerging Applications (IWNET-2017)  2017.9 

     More details

    Language:English   Presentation type:Oral presentation(invited, special)  

    Venue:Xi’an Jiaotong University, Xi’an China  

  • 透析液管理のためのエンドトキシンセンサ Invited

    井上, 安田) 久美

    日本分析化学会第66年会  2017.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:東京理科大学葛飾キャンパス  

  • 糞便成分センサーを利用した健康検知トイレの開発 Invited

    山崎聖司, 井上久美

    JSTフェア2017(シンポジウム)  2017.9 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • バイオ・ヘルスケア Invited

    INOUE Kumi Y

    MEMS集中講義 in 川崎  2017.8 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • Liquid-junction free substitutional stripping voltammetry based on a closed bipolar electrode system and its application to endotoxin sensor International conference

    Kumi Y. Inoue, Shinichiro Takano, Miho Ikegawa, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    XXIV International Symposium on Bioelectrochemistry and Bioenergetics (Bioelectrochemistry 2017)  2017.7 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:La Doua Campus of Claude Bernard University Lyon 1  

  • フェニレンジアミン誘導体を用いたプロテアーゼ基質の開発と電気化学的エンドトキシン検出への応用

    井上(安田)久美, 鎌田 祐麿, 孫 思祥, 池川 未歩, 伊藤, 佐々木 隆広, 末永 智一

    電気化学会第84回大会  2017.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:首都大学東京南大沢キャンパス,東京  

  • Close型バイポーラ電極システムを用いる参照電極の液絡を必要としない多点アンペロメトリック検出法の開発

    井上(安田)久美, 池川未歩, 伊藤-佐々木隆広, 伊野浩介, 珠玖仁, 末永智一

    第62 回ポーラログラフィーおよび電気分析化学討論会  2016.11 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:沖縄県宮古島市中央公民館大ホール,沖縄  

  • 電気化学的カスパーゼ3活性測定による簡易な細胞アポトーシスモニタリング

    井上(安田)久美, 孫 思祥, 塩本 周作, 高野 真一朗, 伊野 浩介, 珠玖 仁, 末永 智一

    日本分析化学会第65年会  2016.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:北海道大学 札幌  

  • Liquid-junction Free Substitutional Stripping Voltammetry Using a Closed Bipolar Electrode System. International conference

    Kumi Y. Inoue, Shinichiro Takano, Miho Ikegawa, Yasufumi Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    The 16th International Meeting on Chemical Sensors (IMCS2016)  2016.7 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Ramada Plaza Jeju, Jeju Island, Korea  

  • 細胞呼吸活性評価のための高空間分解能バイオ LSI の開発

    井上(安田)久美, 橋 由佳, 伊野浩介, 珠玖 仁, 末永智一

    電気化学会第83回大会・第60回化学センサ研究発表会  2016.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 電気化学バイオセンサの高機能化 Invited

    INOUE Kumi Y

    電気学会・先進ナノ構造制御と高性能有機デバイス・ライフサイエンス応用調査専門委員会  2016.3 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • (東北分析化学賞受賞講演)小型チップデバイスによる電流検出式バイオセンシング法の開発に関する研究 Invited

    INOUE Kumi Y

    日本分析化学会東北支部役員会  2015.12 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • CMOS-based amperometric sensor array for electrochemical detection of cell respiratory activity. International conference

    K. Y. Inoue, Y. Hashi, K. Takara, Y. Takahashi, M. Matsudaira, R. Kunikata, A. Suda, K. Ino, H. Shiku, T. Matsue

    11th Asian Conference on Chemical Sensors (ACCS2015)  2015.11 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Penang, Malaysia  

  • バイポーラー電極と駆動電極を用いたアンペロメトリー計測に関する検討

    井上(安田) 久美, 高野 真一朗, 池川 未歩, 伊野 浩介, 珠玖 仁, 末永 智一

    2015年電気化学秋季大会  2015.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:深谷  

  • lectrochemical Approach to Detect Protease Activities and Its Application for a Simple Apoptosis Sensor. International conference

    Kumi Y. Inoue, Shinichiro Takano, Shusaku Shiomoto, Sixiang Sun, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    XXIII International Symposium on Bioelectrochemistry and Bioenergetics of the Bioelectrochemical Society  2015.6 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Sweden Malmö  

  • メトキシアニリンを遊離する新規カスパーゼ3 基質の評価と細胞アポトーシス検出デバイスへの応用

    井上(安田)久美, 塩本周作, 髙野真一朗, 孫 思祥, 伊野浩介, 珠玖 仁, 末永智一

    電気化学会第82回大会  2015.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:横浜  

  • Electrochemical detection techniques to provide a portable and highly sensitive endotoxin sensor Invited International conference

    INOUE Kumi Y

    UK-Japan Workshop on BioSensing Technologies for the Innovative Healthcare  2014.12 

     More details

    Language:English   Presentation type:Other  

    Venue:英国大使館,東京  

  • BioLSI for highly sensitive electrochemical biosensors International conference

    UK-Japan Workshop on BioSensing Technologies for the Innovative Healthcare  2014.12 

     More details

    Language:English   Presentation type:Symposium workshop panel(nominated)  

    Venue:Tokyo  

  • 集積型アンペロメトリックセンサアレイ「バイオLSI」の高感度化のためのデータ取得システム

    井上 久美, 松平 昌昭, 伊野 浩介, 珠玖 仁, 末永 智一

    第60回ポーラログラフィーおよび電気分析化学討論会  2014.11 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:京都  

  • エンドトキシンセンサ Invited

    Kumi Y.Inoue

    生物化学的測定研究会第19回学術シンポジウム  2014.11 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:仙台  

  • カスパーゼ3の電気化学検出法の開発と細胞アポトーシスアッセイへの応用

    塩本 周作, 高野 真一朗, 井上(安田)久美, 伊野 浩介, 珠玖 仁, 末永 智一

    第57回化学センサ研究発表会  2014.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:札幌  

  • Biosensing and bioimaging with integrated electrochemical sensor arrays International conference

    Kumi Inoue, Tomokazu Matsue

    AIMR TohokuUniversity-NCTU Joint Workshop  2014.9 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Taiwan Hsinchu  

  • Improvement of LSI-based amperometric sensor array for wide application of bioimaging and biosensing. International conference

    Kumi Y. Inoue, Masahki Matsudaira, Kosuke Ino, Masanori Nakano, Kosuke Takara, Atsushi Suda, Ryota Kunikata, Shinya Yoshida, Takeshi Hayasaka, Yoshiyuki Kikuchi, Xijiang Chang, Tomohiro Kubota, Hitoshi Shiku, Shuji Tanaka, Seiji Samukawa, Tomokazu Matsue

    65th Annual Meeting of the International Society of Electrochemistry  2014.8 

     More details

    Language:English   Presentation type:Poster presentation  

    Venue:Switzerland Lausanne  

  • アンペロメトリックバイオイメージングプラットフォーム「バイオLSI」

    井上(安田)久美, 松平昌昭, 伊野浩介, 中野将識, 菅野佑介, 須田篤史, 國方亮太, 吉田慎哉, 早坂丈, 菊地良幸, Xijiang Chang, 久保田智広, 珠玖仁, 田中秀治, 寒川誠二, 末永智一

    第74回分析化学討論会  2014.5 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:郡山  

  • 産業の発展に資するセンシング原理および電気化学センサデバイス構築に関する研究 Invited

    井上(安田)久美

    電気化学会第81回大会  2014.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:吹田  

    電気化学会女性躍進賞受賞講演

  • Integration of LSI Technology to Advanced Electrochemical Array Sensor and Its Application for Biomedical Assays. International conference

    Kumi Y. Inoue, Kosuke Ino, Kosuke Takara, Mustafa Şen, Shinichiro Takano, Reyushi Kubo, Masanori Nakano, Shusaku Shiomoto, Yuka Hashi, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue

    The 10th Asian Conference on Chemical Sensors  2013.11 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Thailand Chiang Mai  

  • Bioimaging Using LSI-based Amperometric Biosensing System with 400- Electrodes. International conference

    Kumi Y. Inoue, Kosuke Ino, Mustafa Şen, Masanori Nakano, Kosuke Takara, Reyushi Kubo, Masahki Matsudaira, Atsushi Suda, Ryota Kunikata, Hitoshi Shiku, Tomokazu Matsue

    224th ECS Meeteing  2013.10 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:United States San Francisco  

  • プロテアーゼ活性の電気化学測定法の開発と医療応用を目指したアポトーシスおよびエンドトキシンの検出

    井上(安田)久美, 髙野真一朗, 塩本周作, 石田洋祐, 中村文哉, 伊野浩介, 珠玖仁, 末永智一

    日本分析化学会 第62年会  2013.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:大阪  

  • Endotoxin assay on electrode chip for portable and easy- to- use sensor system

    Kumi Y. Inoue, Shinichiro Takano, Satoko Takahashi, Yosuke Ishida, Takahiro Ito-Sasaki, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    The 12th Biennial International Endotoxin & Innate Immunity Society (IEIIS) meeting  2012.10 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:JAPAN Tokyo  

  • 超高感度電流検出型センサアレイ『バイオLSI』- 医療,生命科学へのLSIセンサ利用にむけて- Invited

    INOUE Kumi Y

    マイクロシステム融合研究開発拠点 先端融合推進研究会(第33回)  2012.10 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • バイオLSI ラベルフリーでバイオ分子の挙動をリアルタイムに画像化.同時多項目検査チップにも.

    INOUE Kumi Y

    BioJapan 2012 出展者セミナー  2012.10 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

  • 集積型多点バイオセンサ「超高感度電流検出型バイオLSI計測システム」の開発

    井上久美, 松平昌昭, 久保礼有志, 須田篤史, 國方亮太, 木村龍男, 鶴見亮太, 塩谷俊人, 伊野浩介, 珠玖仁, 江刺正喜, 末永智一

    日本分析化学会第61年会  2012.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:金沢  

  • Development of Electrochemical Detection Method for Highly-Sensitive Endotoxin Assay Based on Zymogen Activation Cascade Reactions. International conference

    Kumi Y. Inoue, Satoko Takahashi, Hayato Kodama, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    63rd Annual Meeting of the International Society of Electrochemistry  2012.8 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Czech Republic Prague  

  • 酵素前駆体を用いる電気化学エンドトキシンセンサのための高感度検出法の検討

    井上(安田)久美, 高橋 里子, 児玉 隼人, 伊野 浩介, 珠玖 仁, 末永 智一

    電気化学会第79回大会  2012.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:浜松  

  • ZYMOGEN BASED ELECTROCHEMICAL SENSOR FOR ENDOTOXIN ASSAY International conference

    Satoko Takahashi, Kosuke Ino, Hitoshi Shiku, Tomokazu Matsue

    The 9th Asian Conference on Chemical Sensors  2011.11 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Taiwan Chientan Youth Activity Center, Taipei  

    ACCS Best Paper Presentation award受賞

  • Zymogen-based Biosensors and Its Application for Highly Sensitive Endotoxin Detection on a Chip Device International conference

    K. Y. Inoue, S. Takahashi, K. Ino, H. Shiku, T. Matsue

    62nd Annual Meeting of the International Society of Electrochemistry  2011.9 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:JAPAN 新潟  

  • リムルス試薬を用いた電気化学検出型の超高感度小型エンドトキシン検査装置の開発

    高橋 里子, 伊野 浩介, 珠玖 仁, 末永 智一

    電気化学会第78回大会(横浜国立大学)  2011.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:横浜国立大学  

    震災のため大会中止.要旨集での発表のみ.

  • 産々学連携で世の中に役立つ成果を創る 超高感度多点電流検出型バイオLSIの開発

    INOUE Kumi Y

    国際計量計測展2010 大学・研究機関コーナープレゼンテーション  2010.11 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

    Venue:ビッグサイト,東京  

  • 「超高感度多点電流検出型バイオLSI」 - 医療,生命科学へのLSIセンサ利用にむけて – Invited

    INOUE Kumi Y

    マイクロシステム融合研究開発拠点 先端融合推進研究会(第30回)  2010.10 

     More details

    Language:Japanese   Presentation type:Public discourse, seminar, tutorial, course, lecture and others  

    Venue:東北大学,仙台  

  • Zymogen-based Electrochemical Sensor for Endotoxin Using Recombinant Factor C. International conference

    61st Annual Meeting of the International Society of Electrochemistry  2010.9 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:France Nice Acropolis  

  • 酵素前駆体(組換えFactor C)を利用するエンドトキシンの電気化学測定法の検討

    伊野 浩介, 珠玖 仁, 末永 智一

    電気化学会第77回大会  2010.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:富山大 五福  

  • Development of highly-sensitive electrochemical biosensors for biomolecules using enzymes International conference

    Kumi Y. Inoue

    G-COE Dalian University of Technology-Tohoku University Joint Symposium  2009.12 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:JAPAN 東北大学川内萩ホール  

  • Cell-based amperometric biosensor for the rapid sensing of toxicants using the leukocytes and monitoring the release of hydrogen peroxide. International conference

    K. Ino, H. Shiku, S. Kasai, T. Yasukawa, F. Mizutani, T. Matsue

    8th Asian Conference on Chemical Sensors  2009.11 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Republic of Korea Exco, Daegu  

  • 過酸化水素放出を指標とする白血球細胞バイオセンサの開発

    伊野 浩介, 珠玖 仁, 葛西 重信, 安川 智之, 水谷 文雄, 末永 智一

    電気化学会第76回大会  2009.3 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:京大 吉田  

  • アルカリフォスファターゼレポーターを用いたエンドトキシン検出のための細胞バイオセンサー

    安川 智之, 珠玖 仁, 末永 智一

    第3回生体関連化学合同シンポジウム  2008.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:東工大 すずかけ台  

  • Cell-Based Biosensor for Endotoxin Assay Using a Secreted Alkaline Phosphatase Reporter System International conference

    T. Yasukawa, H. Shiku, T. Matsue

    3rd Chemical-Environmental International Student Workshop 2008  2008.8 

     More details

    Language:English   Presentation type:Oral presentation(general)  

    Venue:Taiwan National Tsing Hua University  

  • 細胞を利用するエンドトキシンのセンシング

    安川 智之, 珠玖 仁, 末永 智一

    2007年 電気化学秋季大会  2007.9 

     More details

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:東工大 大岡山  

▼display all

The total number of announcement other than the above

  • 2020

    Total number of non-preannounced events:1  Number of main presenters:1  Number of presenters other than main person:0

Industrial Property Rights

  • 構造体の製造方法及び構造体の製造装置

    久保田 恒喜、遠藤 彩音、戸塚 友理、井上 久美

     More details

    Application no:特願2022-135338  Date applied:2022.8

    Country of applicant:Domestic  

  • エンドトキシン検出装置、及びエンドトキシンの検出方法

    伊藤隆広,井上久美,伊藤健太郎,珠玖仁,末永智一

     More details

    Application no:特願 2020-533044  Date applied:2020.3

    Publication no:再表2020/184482  Date published:2020.9

    Country of applicant:Foreign country  

  • エンドトキシン検出装置、及びエンドトキシンの検出方法

    伊藤 隆広, 井上 久美, 伊藤 健太郎, 珠玖 仁, 末永 智一

     More details

    Applicant:国立大学法人東北大学

    Application no:JP2020009859  Date applied:2020.3

    Publication no:WO2020-184482  Date published:2020.9

    J-GLOBAL

  • エンドトキシン検出装置、及びエンドトキシンの検出方法

    伊藤 隆広, 井上 久美, 伊藤 健太郎, 珠玖 仁, 末永 智一

     More details

    Applicant:国立大学法人東北大学

    Application no:JP2020009859  Date applied:2020.3

    Publication no:WO2020-184482  Date published:2020.9

    Patent/Registration no:特許第6795245号  Date registered:2020.11 

    J-GLOBAL

  • 携帯型検査システム、アプタマーシート、および携帯型検査デバイス

    金子直人, 堀井克紀, 和賀 巌, 田中秀治,塚本貴城,井上久美,伊藤隆広,アブデリ ハムザ

     More details

    Application no:特願2018-198596  Date applied:2018.10

    Announcement no:特開2020-067300  Date announced:2020.4

    Country of applicant:Domestic  

  • 電気化学センサ及び電気化学センサの製造方法

    末永智一,井上久美,岩間智紀,阿部博弥

     More details

    Application no:特願2018-157868  Date applied:2018.8

    Announcement no:特開2020-030192  Date announced:2020.2

    Patent/Registration no:6757079  Date registered:2020.9 

    Country of applicant:Domestic  

  • 電気化学センサ及び電気化学センサの製造方法

    末永智一, 井上久美, 岩間智紀, 阿部博弥

     More details

    Applicant:国立大学法人東北大学

    Application no:特願2018-157868  Date applied:2018.8

    Announcement no:特開2020-030192  Date announced:2020.2

    Patent/Registration no:特許6757079  Date registered:2020.9 

    J-GLOBAL

  • 電気化学センサ及び電気化学センサの製造方法

    末永 智一, 井上 久美, 岩間 智紀, 阿部 博弥

     More details

    Applicant:国立大学法人東北大学

    Application no:特願2018-157868  Date applied:2018.8

    Announcement no:特開2020-030192  Date announced:2020.2

    Patent/Registration no:特許第6757079号  Date registered:2020.9 

    J-GLOBAL

  • アルドステロン及びレニンの検出方法

    佐藤 文俊, 藪 浩, 井上 久美, 佐藤 さつき, 根本 靖久, 前田 郁麻

     More details

    Applicant:株式会社 ハプロファーマ, 国立大学法人東北大学

    Application no:JP2018016262  Date applied:2018.4

    Publication no:WO2018-194152  Date published:2018.10

    J-GLOBAL

  • 多相ポリマー微粒子を用いた検体物質の検出方法

    佐藤 文俊, 藪 浩, 井上 久美, 佐藤 さつき, 珠玖 仁, 根本 靖久, 前田 郁麻

     More details

    Applicant:株式会社 ハプロファーマ, 国立大学法人東北大学

    Application no:JP2018016261  Date applied:2018.4

    Publication no:WO2018-194151  Date published:2018.10

    J-GLOBAL

  • エンドトキシン検出用チップおよびエンドトキシン濃度の定量方法

    樋口拓也,小川健一,末永智一,井上久美,高野真一朗

     More details

    Application no:特願2017-197217  Date applied:2017.10

    Announcement no:特開 2018-72331  Date announced:2018.5

  • 多相ポリマー微粒子を用いた検体物質の検出方法

    佐藤文俊,藪浩,井上久美,佐藤さつき,珠玖仁,根本靖久,前田郁麻

     More details

    Application no:特願2017-084812  Date applied:2017.4

    Publication no:再表2018/194151  Date published:2018.10

    Country of applicant:Foreign country  

  • アルドステロン及びレニンの検出方法

    佐藤文俊, 藪浩, 井上久美, 佐藤さつき, 根本靖久, 前田郁麻

     More details

    Application no:特願2017-084764  Date applied:2017.4

    Publication no:WO2018-194152  Date published:2018.10

    J-GLOBAL

  • 多相ポリマー微粒子を用いた検体物質の検出方法

    佐藤文俊, 藪浩, 井上久美, 佐藤さつき, 珠玖仁, 根本靖久, 前田郁麻

     More details

    Application no:特願2017-084812  Date applied:2017.4

    Publication no:WO2018-194151  Date published:2018.10

    J-GLOBAL

  • Electrochemical measurement using phenylenediamine derivative

    Mizumura Hikaru,Oda Toshio,Matsue Tomokazu,Inoue Kumi

     More details

    Application no:特願2018-500152  Date applied:2017.2

    Publication no:再表2017/141961  Date published:2017.8

    Country of applicant:Foreign country  

  • エンドトキシン検出用チップ

    樋口拓也, 小川健一, 末永智一, 井上久美, 高野真一朗

     More details

    Application no:特願2016-208519  Date applied:2016.10

  • 電気化学測定装置及びトランスデューサ

    國方亮太,林泰之,須田篤史,伊野浩介,井上久美,末永智一

     More details

    Application no:特願2016-197818  Date applied:2016.10

    Announcement no:特開2018-059823  Date announced:2018.4

    Patent/Registration no:特許第6218199号  Date registered:2017.10 

  • 電気化学測定装置及びトランスデューサ

    國方亮太, 林泰之, 須田篤史, 伊野浩介, 井上久美, 末永智一

     More details

    Application no:特願2016-197818  Date applied:2016.10

  • 電気化学イメージング方法、電気化学測定装置及びトランスデューサ

    伊野浩介,菅野佑介,末永智一,井上久美,國方亮太,林泰之,須田篤史

     More details

    Application no:特願2016-101043  Date applied:2016.5

    Announcement no:特開2017-207410  Date announced:2017.11

    Patent/Registration no:特許第6344775号  Date registered:2018.6 

    Country of applicant:Foreign country  

  • 電気化学イメージング方法、電気化学測定装置及びトランスデューサ

    伊野浩介, 菅野佑介, 末永智一, 井上久美, 國方亮太, 林泰之, 須田篤史

     More details

    Application no:特願2016-101043  Date applied:2016.5

  • 電気化学測定方法、電気化学測定装置及びトランスデューサ

    林泰之,國方亮太,須田篤史,伊野浩介,井上久美,末永智一

     More details

    Application no:特願2016-087924  Date applied:2016.4

    Announcement no:特開2017-198493  Date announced:2017.11

    Patent/Registration no:特許第61160806号  Date registered:2017.3 

    Country of applicant:Domestic  

  • 電気化学測定方法、電気化学測定装置及びトランスデューサ

    林泰之, 國方亮太, 須田篤史, 伊野浩介, 井上久美, 末永智一

     More details

    Application no:特願2016-087924  Date applied:2016.4

  • 電極構造の製造方法、電気化学センサの製造方法、電極構造および電気化学センサ

    樋口拓也,渡辺正直,末永智一,井上久美

     More details

    Application no:特願2016-056567  Date applied:2016.3

    Announcement no:特開2017-173014  Date announced:2017.9

  • 電極構造の製造方法、電気化学センサの製造方法、電極構造および電気化学センサ

    樋口拓也, 渡辺正直, 末永智一, 井上久美

     More details

    Application no:特願2016-056567  Date applied:2016.3

  • 電極チップ

    末永智一,井上久美,伊藤隆広,珠玖仁,池川未歩

     More details

    Application no:特願2016-055251  Date applied:2016.3

    Announcement no:特開2017-167104  Date announced:2017.9

    Patent/Registration no:特許第6579325号  Date registered:2019.9 

  • 電気化学測定方法、電気化学測定装置及びトランスデューサ

    國方 亮太, 林 泰之, 須田 篤史, 伊野 浩介, 井上 久美, 末永 智一

     More details

    Applicant:日本航空電子工業株式会社, 国立大学法人東北大学

    Application no:特願2016-055283  Date applied:2016.3

    Announcement no:特開2017-116517  Date announced:2017.6

    J-GLOBAL

  • 電極チップ

    末永智一, 井上久美, 伊藤隆広, 珠玖仁, 池川未歩

     More details

    Application no:特願2016-055251  Date applied:2016.3

    Announcement no:特開2017-167104  Date announced:2017.9

    Patent/Registration no:特許第6579325号  Date registered:2019.9 

    J-GLOBAL

  • 電極構造の製造方法、センサ電極の製造方法、電極構造およびセンサ電極

    樋口拓也,末永智一,井上久美

     More details

    Application no:特願2016-018358  Date applied:2016.2

    Announcement no:特開2017-138164  Date announced:2017.8

  • 電極構造の製造方法、センサ電極の製造方法、電極構造およびセンサ電極

    樋口拓也, 末永智一, 井上久美

     More details

    Application no:特願2016-018358  Date applied:2016.2

    Announcement no:特開2017-138164  Date announced:2017.8

    J-GLOBAL

  • 電気化学測定方法、電気化学測定装置及びトランスデューサ

    國方亮太, 林泰之, 須田篤史, 伊野浩介, 井上久美, 末永智一

     More details

    Application no:特願2015-249764  Date applied:2015.12

    Announcement no:特開2017-116517  Date announced:2017.6

  • 電気化学測定方法、電気化学測定装置及びトランスデューサ

    林泰之, 國方亮太, 須田篤史, 伊野浩介, 井上久美, 末永智一

     More details

    Application no:特願2015-228042  Date applied:2015.11

    Patent/Registration no:特許6116075  Date issued:2017.3

  • 電気化学式エンドトキシン濃度検出用容器、電極チップおよびエンドトキシンの検出方法

    樋口拓也,渡辺正直,末永智一,井上久美,尹善愛

     More details

    Application no:特願2015-159962  Date applied:2015.8

    Announcement no:特開2017-37044  Date announced:2017.2

    Patent/Registration no:特許第6562765号  Date registered:2019.8 

    Country of applicant:Domestic  

  • 電極チップおよび電極チップの製造方法

    樋口 拓也, 渡辺 正直, 末永 智一, 井上 久美, 尹 善愛

     More details

    Applicant:大日本印刷株式会社, 国立大学法人東北大学

    Application no:特願2015-159962  Date applied:2015.8

    Announcement no:特開2017-037044  Date announced:2017.2

    Patent/Registration no:特許第6557090号  Date registered:2019.7 

    J-GLOBAL

  • 電極構造の製造方法、センサ電極の製造方法、電極構造およびセンサ電極

    樋口拓也,末永智一,井上久美

     More details

    Application no:特願2015-159963  Date applied:2015.8

    Announcement no:特開2017-37045  Date announced:2017.2

    Patent/Registration no:特許第6514991号  Date registered:2019.4 

  • 電気化学式エンドトキシン濃度検出用容器、電極チップおよびエンドトキシンの検出方法

    樋口 拓也, 渡辺 正直, 石井 睦, 末永 智一, 井上 久美, 尹 善愛

     More details

    Applicant:大日本印刷株式会社, 国立大学法人東北大学

    Application no:特願2015-159960  Date applied:2015.8

    Announcement no:特開2017-037043  Date announced:2017.2

    Patent/Registration no:特許第6562765号  Date registered:2019.8 

    J-GLOBAL

  • 電極構造の製造方法、センサ電極の製造方法、電極構造およびセンサ電極

    樋口拓也, 末永智一, 井上久美

     More details

    Application no:特願2015-159963  Date applied:2015.8

    Announcement no:特開2017-37045  Date announced:2017.2

  • 電気化学式エンドトキシン濃度検出用容器、電極チップおよびエンドトキシンの検出方法

    樋口拓也, 渡辺正直, 末永智一, 井上久美, 尹善愛

     More details

    Application no:特願2015-159962  Date applied:2015.8

    Announcement no:特開2017-37044  Date announced:2017.2

    Patent/Registration no:特許第6562765号  Date registered:2019.8 

    J-GLOBAL

  • 微生物夾雑物の濃度検出方法、電極チップおよびオリゴペプチド

    樋口 拓也,小川健一,坪井達也,末永智一,井上久美,高野真一朗

     More details

    Application no:特願2015-47649  Date applied:2015.3

    Announcement no:特開2015-187607  Date announced:2015.10

    Patent/Registration no:特許第5916176号  Date registered:2016.4 

    Country of applicant:Domestic  

  • 電極チップおよび化学物質の定量方法

    小川健一,坪井達也,末永智一,井上久美,高野真一朗

     More details

    Application no:特願2014-070156  Date applied:2014.3

    Announcement no:特開2015-190939  Date announced:2015.11

    Patent/Registration no:特許第6240543号  Date registered:2017.11 

    Country of applicant:Domestic  

  • 電極チップおよび化学物質の定量方法

    小川健一, 坪井達也, 末永智一, 井上久美, 高野真一朗

     More details

    Application no:特願2014-070156  Date applied:2014.3

    Announcement no:特開2015-190939  Date announced:2015.11

    J-GLOBAL

  • 微生物夾雑物の濃度検出方法、電極チップおよびオリゴペプチド

    樋口 拓也, 小川健一, 坪井達也, 末永智一, 井上久美, 高野真一朗

     More details

    Application no:特願2014-047888  Date applied:2014.3

    Announcement no:特開2015-187607  Date announced:2015.10

  • 複数の電極を備えたICチップ

    末永智一,井上久美,松平昌昭,江刺正喜,須田篤史,木村龍男,國方亮太,塩谷俊人,鶴見亮太

     More details

    Applicant:国立大学法人東北大学,日本航空電子工業株式会社,凸版印刷株式会社

    Application no:特願2011-234459  Date applied:2011.10

    Announcement no:特開2013-092437  Date announced:2013.5

    Patent/Registration no:特許第5769020号  Date registered:2015.8 

    Country of applicant:Domestic  

  • 複数の電極を備えたICチップ

    末永智一, 井上久美, 松平昌昭, 江刺正喜, 須田篤史, 木村龍男, 國方亮太, 塩谷俊人, 鶴見亮太

     More details

    Applicant:国立大学法人東北大学,日本航空電子工業株式会社,凸版印刷株式会社

    Application no:2011-234459  Date applied:2011.10

    Announcement no:2013-092437  Date announced:2013.5

  • 電気化学計測チップ用電極装置

    末永智一,井上久美,須田篤史,木村龍男,國方亮太

     More details

    Applicant:日本航空電子工業株式会社,国立大学法人東北大学

    Application no:特願2010-292608  Date applied:2010.12

    Announcement no:特開2012-141173  Date announced:2012.7

    Patent/Registration no:特許第4933657号  Date registered:2012.2 

  • 電気化学計測チップ用電極装置

    末永智一, 井上久美, 須田篤史, 木村龍男, 國方亮太

     More details

    Applicant:日本航空電子工業株式会社,国立大学法人東北大学

    Application no:特願2010-292608  Date applied:2010.12

    Announcement no:特開2012-141173  Date announced:2012.7

    Patent/Registration no:特許4933657  Date issued:2012.2

  • エンドトキシンの濃度検出方法及びエンドトキシン検出用電極チップ

    末永 智一, 井上 久美, 伊野 浩介, 珠玖 仁

     More details

    Applicant:国立大学法人東北大学

    Application no:特願2010-277329  Date applied:2010.12

    Announcement no:特開2012-127695  Date announced:2012.7

    J-GLOBAL

  • エンドトキシンの濃度検出方法及びエンドトキシン検出用電極チップ

    末永智一, 井上久美, 伊野浩介, 珠玖仁

     More details

    Applicant:国立大学法人東北大学

    Application no:2010-277329  Date applied:2010.12

    Announcement no:2012-127695  Date announced:2012.7

  • 液体混合デバイス、液体混合方法及び微量検体検出法

    安川智之,井上久美,珠玖仁,末永智一,吉田博,末竹寿紀

     More details

    Applicant:ニプロ株式会社,国立大学法人東北大学

    Application no:特願2006-186109  Date applied:2006.7

    Announcement no:特開2008-14791  Date announced:2008.1

  • 液体混合デバイス、液体混合方法及び微量検体検出法

    安川智之, 井上久美, 珠玖仁, 末永智一, 吉田博, 末竹寿紀

     More details

    Applicant:ニプロ株式会社,国立大学法人東北大学

    Application no:特願2006-186109  Date applied:2006.7

    Announcement no:特開2008-14791  Date announced:2008.1

  • 電気化学検出器

    加納 健司, 高木 一好, 井上 久美, 池田 篤治, 上田 輝久

     More details

    Applicant:株式会社島津製作所

    Application no:特願平8-167954  Date applied:1996.6

    Announcement no:特開平10-019839  Date announced:1998.1

    Patent/Registration no:特許第3684682号  Date registered:2005.6 

    J-GLOBAL

  • 電気化学検出器

    加納健司, 高木一好, 井上久美

     More details

    Applicant:株式会社島津製作所

    Application no:特願平8-167954  Date applied:1996.6

    Announcement no:特開平10-19839  Date announced:1998.1

▼display all

Awards

  • Encouragement Award by 2023 Excellent Faculty Incentive System

    2023.9   University of Yamanashi  

    Kumi Y. Inoue

  • 第24回酵素応用シンポジウム研究奨励賞

    2023.6   一般社団法人 天野エンザイム科学技術振興財団   プロテアーゼ反応を用いる高感度・小型・簡便な電気化学センサー

    井上 久美

     More details

    Award type:Award from publisher, newspaper, foundation, etc. 

  • 令和4年度 山梨大学男女共同参画学術研究奨励賞優秀賞

    2022.11   山梨大学   バイオをはかる、バイオではかる

  • 令和4年度優秀教員奨励制度による奨励賞

    2022.9   山梨大学  

  • Best poster award

    2022.2  

    Kumi Y. Inoue

  • Shikata Medal

    2018.11   The Polaographic Society Japan  

    INOUE Kumi Y

  • Shikata Medal

    2018.11   The Polaographic Society Japan  

    INOUE Kumi Y

  • 清山賞

    2018.1   公益社団法人 電気化学会 化学センサ研究会   「医療・バイオ分野での利用に資するアンペロメトリックセンシングチップの開発」

     More details

    Award type:International academic award (Japan or overseas)  Country:Japan

  • 清山賞

    2018.1   公益社団法人 電気化学会 化学センサ研究会   「医療・バイオ分野での利用に資するアンペロメトリックセンシングチップの開発」

     More details

    Award type:International academic award (Japan or overseas)  Country:Japan

  • 東北分析化学賞

    2015.12   日本分析化学会東北支部   チップ型電気化学バイオセンシングシステム作製に関する研究

     More details

    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 東北分析化学賞

    2015.12   日本分析化学会東北支部   チップ型電気化学バイオセンシングシステム作製に関する研究

     More details

    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • 電気化学会女性躍進賞

    2014.3   公益社団法人電気化学会   産業の発展に資するセンシング原理および電気化学センサデバイス構築に関する研究

     More details

    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  • ACCS Best Paper Presentation award

    2011.11   The 9th Asian Conference on Chemical Sensors organizing committee   ZYMOGEN BASED ELECTROCHEMICAL SENSOR FOR ENDOTOXIN ASSAY

     More details

    Award type:Award from international society, conference, symposium, etc. 

▼display all

Teaching Experience (On-campus)

  • Advanced Chemical Analysis

    2023Year

  • Basic Analytical Chemistry Major achievement

    2023Year

  • Laboratory of Chemistry Major achievement

    2023Year

  • Chemistry Major achievement

    2023Year

  • 応用化学実験Ⅳ

    2023Year  Type of subject:Professional education (undergraduate)

  • 応用化学卒業論文

    2023Year  Type of subject:Professional education (undergraduate)

  • ものづくり基礎ゼミ

    2023Year  Type of subject:Professional education (undergraduate)

  • ものづくり発展 ゼミⅠ

    2023Year  Type of subject:Professional education (undergraduate)

  • 応用化学基礎ゼミ

    2023Year  Type of subject:Professional education (undergraduate)

  • Advanced Chemical Analysis

    2022Year

  • Basic Analytical Chemistry Major achievement

    2022Year

  • Laboratory of Chemistry Major achievement

    2022Year

  • Chemistry Major achievement

    2022Year

  • 応用化学基礎ゼミ

    2022Year  Type of subject:Professional education (undergraduate)

  • ものづくり発展 ゼミⅠ

    2022Year  Type of subject:Professional education (undergraduate)

  • Chemical Experiments

    2022Year

  • Chemistry Major achievement

    2022Year

  • 応用化学実験Ⅳ

    2022Year  Type of subject:Professional education (undergraduate)

  • ものづくり基礎 ゼミ

    2022Year  Type of subject:Professional education (undergraduate)

  • ものづくり発展 ゼミⅡ

    2022Year  Type of subject:Professional education (undergraduate)

  • 応用化学卒業論文

    2022Year  Type of subject:Professional education (undergraduate)

  • Advanced Analytical Chemistry

    2022Year

  • ものづくり発展 ゼミⅡ

    2022Year  Type of subject:Professional education (undergraduate)

  • ものづくり基礎ゼミ

    2021Year  Type of subject:Professional education (undergraduate)

  • 応用化学基礎ゼミ

    2021Year  Type of subject:Professional education (undergraduate)

▼display all

Teaching Experience

  • 生命センシング

    Institution:東北大学

  • 創造工学研修

    Institution:東北大学

  • 生体情報化学

    Institution:東北大学

  • 化学・バイオ工学実験

    Institution:東北大学

  • 化学基礎

    Institution:東北工業大学

  • 化学基礎実験

    Institution:東北工業大学

  • 化学への旅

    Institution:東北工業大学

  • 応用環境学概論

    Institution:東北大学

  • 生命センシング

    Institution:東北大学

  • 生体情報化学

    Institution:東北大学

  • 応用環境学概論

    Institution:東北大学

  • 化学基礎実験

    Institution:東北工業大学

  • 化学基礎

    Institution:東北工業大学

  • 化学・バイオ工学実験

    Institution:東北大学

  • 化学への旅

    Institution:東北工業大学

  • 創造工学研修

    Institution:東北大学

▼display all

Teaching achievements

  • ナシダイResearchers vol.9 掲載

    2022.02.28

Other educational achievements

  • フィロス化学を開講し、月曜17:00-19:00オンライン質問を受け付けた

    2023

Review of master's and doctoral thesis

  • 2023

    Examiner classification:Second reader

    Master :2people 

  • 2022

    Examiner classification:Second reader

    Master :2people 

Social Activities

  • 10年遅れのキャリア

    Role(s): Contribution

    全国ダイバーシティネットワーク  コラム 女性研究者・学生の声  WEB  2023.2

     More details

    Audience: Researchesrs, General

    Type:Internet

  • ガールズサイエンスcafe2022 山梨大学教員3名による自身のキャリア・研究紹介(インタビュー)

    Role(s): Appearance, Advisor

    山梨大学男女共同参画推進室  ガールズサイエンスcafe2022  オンライン(動画配信)  2022.11 - 2022.12

     More details

    Audience: Junior students, High school students, Guardians

    Type:Science cafe

  • 山梨大学発ビジネスチャンス直行便 No.2021-3

    Role(s): Informant

    山梨中央銀行 コンサルティング営業部  山梨大学発 “ビジネスチャンス”直行便!  2022.3

  • 令和3年度女子中高生と保護者のための理工系Web講座

    Role(s): Lecturer

    2021.10 - 2022.3

     More details

    Audience: Junior students, High school students

    Type:Other

  • ナシダイResearchers Vol.9

    Role(s): Media coverage

    2021.8 - 2022.3

     More details

    Audience: Junior students, High school students

    Type:Promotional material

  • 第31回日本MRS年次大会シンポジウムオーガナイザー

    Role(s): Organizing member

    2021.8 - 2022.3

  • 第4回COI学術交流会実行委員長

    Role(s): Presenter, Organizing member, Report writing

    2021.4 - 2022.3

  • ヘルスケアや環境計測に役立つ高感度簡易バイオセンサと生命科学研究のための新しいバイオイメージングシステム

    Role(s): Appearance, Informant

    AREC技術紹介セミナー  2021.2

  • ヘルスケアや環境計測に役立つ高感度簡易バイオセンサと生命科学研究のための新しいバイオイメージングシステム

    Role(s): Appearance, Informant

    令和2年度やまなし産学官連携研究交流事業  2020.11

▼display all

Professional Memberships

  • The Electrochemical Society of Japan

  • The Japan Society for Analytical Chemistry

  • The Polarographic Society of Japan

Committee Memberships

  • 第32回日本MRS年次大会シンポジウム   第33回日本MRS年次大会シンポジウムオーガナイザー  

    2023.10 - 2024.3   

      More details

    Committee type:Society

  • 公益社団法人 電気化学会 関東支部   電気化学会関東支部幹事  

    2023.4   

      More details

    Committee type:Society

  • Asian Conference on Chemical Sensors   Asian Conference on Chemical Sensors International Steering Committee Member  

    2023.4   

  • 公益社団法人 電気化学会 男女共同参画推進委員会   男女共同参画推進委員会 副委員長  

    2023.4 - 2024.4   

      More details

    Committee type:Society

  • 文部科学省 科学技術・学術政策研究所 科学技術予測センター   専門調査員  

    2023.4 - 2024.3   

      More details

    Committee type:Government (national level)

  • 公益社団法人電気化学会   広報委員会 委員  

    2023.3 - 2024.3   

      More details

    Committee type:Society

  • 公益社団法人電気化学会   編集委員 幹事  

    2023.3 - 2024.3   

      More details

    Committee type:Society

  • 公益社団法人 電気化学会 化学センサ研究会   令和5年度化学センサ研究会役員(編集委員)  

    2023.1   

  • 日本ポーラログラフ学会   日本ポーラログラフ学会理事・評議員  

    2023.1   

  • 第32回日本MRS年次大会シンポジウム   第32回日本MRS年次大会シンポジウムオーガナイザー  

    2022.10 - 2023.3   

      More details

    Committee type:Society

  • 公益社団法人 電気化学会 男女共同参画推進委員会   男女共同参画推進委員会 委員  

    2022.4 - 2023.4   

      More details

    Committee type:Society

  • 文部科学省 科学技術・学術政策研究所 科学技術予測センター   専門調査員  

    2022.4 - 2023.3   

      More details

    Committee type:Government (national level)

  • 公益社団法人 電気化学会 関東支部   2022年度電気化学会関東支部幹事  

    2022.4 - 2023.3   

      More details

    Committee type:Society

  • 公益社団法人電気化学会   編集委員 副幹事  

    2022.3 - 2023.3   

      More details

    Committee type:Society

  • 公益社団法人 電気化学会 化学センサ研究会   令和4年度化学センサ研究会役員  

    2022.1 - 2023.1   

  • 日本ポーラログラフ学会   2022年 日本ポーラログラフ学会理事・評議員  

    2022.1 - 2022.12   

  • 第31回日本MRS年次大会シンポジウム   第31回日本MRS年次大会シンポジウムオーガナイザー  

    2021.8 - 2022.3   

      More details

    Committee type:Society

  • 公益社団法人 電気化学会 男女共同参画推進委員会   男女共同参画推進委員会 委員  

    2021.4 - 2022.4   

      More details

    Committee type:Society

  • 第4回COI学術交流会実行委員会   第4回COI学術交流会実行委員会実行委員長  

    2021.4 - 2022.3   

      More details

    Committee type:Others

  • 公益社団法人 電気化学会 関東支部   2021年度電気化学会関東支部幹事  

    2021.4 - 2022.3   

      More details

    Committee type:Society

  • 一般社団法人 日本MRS   第30回日本MRS年次大会 シンポジウムオーガナイザー  

    2020.6 - 2021.3   

  • 公益社団法人 電気化学会 化学センサ研究会、令和2年度化学センサ研究会   役員  

    2020.2   

  • 日本ポーラログラフ学会、2020年 日本ポーラログラフ学会   理事・評議員  

    2020.1   

  • 日本電気化学会   編集委員  

    2019.3   

      More details

    Committee type:Society

  •   第28回MRS-J年次大会 B-5シンポジウム Co-organizer  

    2018.12   

      More details

    Committee type:Society

  •   日本分析化学会第67年会(仙台)実行委員  

    2018.9   

      More details

    Committee type:Society

  •   分析化学誌編集委員  

    2018.3 - 2020.2   

      More details

    Committee type:Society

  •   電気化学会東北支部役員  

    2017.11   

      More details

    Committee type:Society

  •   COI2021会議(北海道⼤学フード&メディカルイノベーション国際拠点)実行委員  

    2017.3   

      More details

    Committee type:Others

  •   IEEE NANO 2016 (16th INTERNATIONAL CONFERENCE ON NANOTECHNOLOGY) (仙台国際センター)実行委員  

    2016.8   

      More details

    Committee type:Society

  •   Rev. Polarogr. 誌編集委員(Editorial Board)  

    2016.4   

      More details

    Committee type:Society

  •   日本分析化学会東北支部役員  

    2015.12   

      More details

    Committee type:Society

  •   第74回分析化学討論会(日本大学工学部)実行委員  

    2014.5   

      More details

    Committee type:Society

  • 化学グランプリ   二次選考運営委員  

    2013.8   

      More details

    Committee type:Others

  • 化学グランプリ   二次選考運営委員  

    2013.7 - 2013.12   

      More details

    Committee type:Others

  • 第74回分析化学討論会   実行委員  

    2013.7 - 2013.9   

      More details

    Committee type:Society

  • 電気化学会第80回記念大会   実行委員  

    2012.3   

      More details

    Committee type:Society

  • 電気化学会第80回記念大会   実行委員  

    2012.3   

      More details

    Committee type:Society

  • The 5th Japanese-German-Italian Meeting of Electrochemists   実行委員  

    2010.10   

      More details

    Committee type:Others

  • The 5th Japanese-German-Italian Meeting of Electrochemists   実行委員  

    2010.10   

      More details

    Committee type:Society

▼display all

Media Coverage

  • BEM電極素子熱延伸法で作製 Newspaper, magazine

    日刊工業新聞社  日刊工業新聞  21面  2021.11

  • LSIとMEMSを融合 バイオLSI実現 高感度電極を400個集積 東北大など産学チーム Newspaper, magazine

    科学新聞  2012.7

  • 生体分子の分布可視化 観察用LSI開発 東北大など Newspaper, magazine

    日刊工業新聞  2012.6