Organization
Graduate Faculty of Interdisciplinary Research Faculty of Medicine Basic Science for Clinical Medicine (General Medical Science Center) Professor
Title
Professor
Kawahara Atsuo
Degree
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博士（医学） ( 1995.3 大阪大学 )
Research Areas
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Life Science / Developmental biology
Research Projects
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ゲノム編集技術が生命科学研究と社会生活に与える衝撃
Grant number：24HT0090 2024.4 - 2025.3
独立行政法人日本学術振興 研究成果公開促進費 ひらめき☆ときめきサイエンス~ようこそ大学の研究室へ~KAKENHI
Authorship：Principal investigator Grant type：Competitive Type of fund：：Science research expense
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モデル生物等研究コーヂィネーチィングネットワークによる希少・未診断疾患の病因遺伝子変異候補の機能解析研究
Grant type：Competitive Type of fund：：Science research expense
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Establishment of a new disease model by congenital p53 hyperactive mutation
Grant number：15K09644 2015.4 - 2018.3
Japan Society for the Promotion of Science University of Yamanashi Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
NAKANE Takaya, SUGITA Kanji, GOI Kumiko, KAWAHARA Atsuo, WAKAYAMA Teruhiko, ABE Masako
We recently experienced a boy with congenital microcephaly, pre- and post- growth failure, immunodeficiency, bone marrow failure. This patient had a p53 mutation, so that the function of p53 protein was enhanced and the specific symptoms were expected to occur. In humans p53 hyperactive mutation has not been reported as far as we could investigate. We created mice with a p53 mutation equivalent to p53 hyperactivity mutation (p53CTD) due to C-terminal deletion found in patients with advanced bone marrow dysfunction by genetic editing technique. p53CTD / WT mice showed a decrease in peripheral lymphocytes and macrocytic anemia in addition to hyperpigmentation of limbs which is a feature of p53 abnormal activation
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Analysis for the regulatory mechanisms underlying the cranial vascular formation
Grant number：26460255 2014.4 - 2017.3
Japan Society for the Promotion of Science Iwate Medical University Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Kimura Eiji, KAWAHARA Atsuo
Previously, we performed microarray analysis of etv2 deficient zebrafish embryos, which was one of the earliest markers for angioblasts. We first identified 165 genes that were up-regulated more than 1.5 fold by silencing etv2 expression, then analyzed their expression patterns at 12-somite stage to evaluate whether these genes were associated with the cranial vascular formation, and finally selected 11 genes for the loss of function assay. In this study, we knocked out these 11 genes using the CRISPR/ Cas9 system to analyze the mechanisms of cerebral vasculature formation. As a result, we succeeded in producing sgRNAs for each gene which was capable of the genome editing. For 10 genes, we established heterozygous zebrafish lines in which targeted genes were knocked out by the frame-shift and analysis of the phenotype induced by loss of function were in progress. Additionally, we succeeded in revealing the primary ocular vasculature formation both in zebrafish and mouse embryos.
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development of genome-editing techniques using engineered nucleases
Grant number：26460088 2014.4 - 2017.3
Japan Society for the Promotion of Science Institute of Physical and Chemical Research Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
Hisano Yu, KAWAHARA Atsuo, KOBAYASHI Takuma
This study aimed to develop new genome-editing techniques using engineered nucleases such as CRISPR/Cas9 system in zebrafish. CRISPR/Cas9 system causes DNA double strand breaks, which can be repaired by non-homologous end joining or homology directed recombination. We achieved to knock-in exogenous genes into target genomic locus using donor vector harboring short homologous sequences. The donor vector was designed to have gRNA-recognition sites, homology arms, and the exogenous gene to be integrated. By introducing two gRNA-recognition sites in both side of the integrating gene, backbone sequences of donor vector can be eliminated. Furthermore, the precise genome modification was also occurred in germ cells, resulting in the establishment of knocked-in zebrafish line. This method enables efficient and precise genome modification in a homology-dependent manner, which should be able to be applied to other model organisms
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Grant number：22116001 2010.4 - 2016.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area) Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
YOKOMIZO Takehiko, AOKI Junken, MURAKAMI Makoto, MAJIMA Masataka, KIYOHARA Yutaka, SUGIMOTO Yukihiko, ARITA Makoto
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脂質メディエーターの生物活性の時空間制御機構
Grant number：22116007 2010
日本学術振興会 科学研究費助成事業 新学術領域研究(研究領域提案型) 新学術領域研究(研究領域提案型)
川原 敦雄
私は、心臓前駆細胞の移動異常により二股心臓の表現型を示すゼブラフィッシュ変異体の機能解析から、原因遺伝子であるSpns2分子がスフィンゴシン-1-リン酸(S1P)の輸送体として機能することを明らかとした。本研究では、初期発生におけるS1Pの生理機能の全貌を明らかにすることを目的としている。平成22年度は、以下の研究成果が得られた。
1. ゼブラフィッシュのS1P合成酵素(SphK1,SphK2)およびS1P受容体(S1PR1-S1PR5)の単離を行った。初期発生における発現パターンを解析した結果、S1P合成酵素は、母性遺伝子として供給されており原腸胚期まで胚全体に発現していることが明らかとなった。これに対して、S1P受容体(S1PR1-S1PR5)は、それぞれ特徴的な発現パターンを示しており、様々な器官形成に関与していることが示唆された。
2. S1Pシグナルと接着分子であるフィブロネクチン(Fn)との遺伝学的な関連性を解析した。Spns2変異体とFn変異体の2重変異体を作製した。Fn変異体は、心臓の形態異常を示すが、正中線への移動異常はほとんど示さない。Spns2変異体の心臓前駆細胞の移動異常は、Fnとの2重変異体とすることによりその移動異常が増強された。このことは、S1Pシグナルとフィブロネクチンが相互に心臓前駆細胞の移動や心臓の形態形成を制御していることを示唆している。
3. Spns2発現抑制胚、S1PR2発現抑制胚およびコントロール胚からRNAを調整し、マイクロアレイ解析を行った。その結果、34遺伝子の発現の挙動がS1Pシグナル異常で大きく変動していた。脂質代謝に関与する遺伝子(アポリポタンパク質やホスホリパーゼC等)も含まれているので、今後、定量PCR法を用いSpns2変異体での発現を調べ、同定された候補遺伝子は、発現抑制実験を行うことによりS1Pシグナルとの関連性を明らかにする。 -
Molecular mechanism of hematopoietic and cardiovascular development in zebrafish
Grant number：20570216 2008 - 2010
Japan Society for the Promotion of Science National Cardiovascular Center Research Institute Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
KAWAHARA Atsuo
I aimed to reveal novel key molecules in vascular and cardiac development by the characterization of novel cardiovascular zebrafish mutants. In the ko095 mutants, aberrant branching of intersegmental vessels besides of the neural tube was observed. Further, the increased expression of VEGF (vascular endothelial growth factor), which is a key factor in angiogenesis, caused the excessive vascular remodeling in the ko095 mutant. I found that the gene responsible for the ko095 is Sars (Seryl-tRNA synthetase) that is known as an enzyme essential for protein synthesis: Sars possesses a novel function involved in angiogenesis. On the other hand, the ko157 mutant exhibited the impairment of cardiac progenitor migration, resulting in two hearts known as cardia bifida. I found that the gene responsible for the ko157 is a twelve-pass transmembrane-domain protein Spns2 (Spnster-like 2). Biochemical analysis of Spns2 presented that Spns2 functions as a transporter of a sphingolipid mediator, sphingosine-1-phosphate (S1P). Thus, I found that Sars and Spns2 play important roles in vascular and cardiac development in zebrafish, respectively.
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Molecular mechanism of the formation and differentiation of hemangioblast
Grant number：16370095 2004 - 2005
Japan Society for the Promotion of Science Kyoto University Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B) Grant-in-Aid for Scientific Research (B)
KAWAHARA Atsuo, HANAOKA Ryuki
We have performed in situ-based screening to identify genes that are specifically expressed in the blood island where both blood and endothelial cells are generated. In this process, we have isolated the heme synthesis enzymes and further examined the function of CPO (coproporphyrinogen oxidase) during zebrafish erythrogenesis. Knockdown analysis of CPO using anti-sense morpholinos (CPO-MO) caused the strong suppression of hemoglobin production, and the maturation of erythorocyte is morphologically arrested. These results suggest that CPO plays important roles in zebrafish erythrogenesis. Next, we examined the regulation of CPO expression. In zebrafish, both GATA1 and biklf transcriptional factors are essential for the blood cell maturation. Interestingly, CPO promoter region has DNA binding sites for both factors. We found that CPO expression is strongly suppressed when biklf-morpholino was injected into vlt mutant embryos (lacking functional GATA1 gene). In contrast, CPO is ectopically induced when both GATA1 and biklf RNAs are injected into two-cell stage embryos. These results suggest that GATA1 and biklf cooperatively contribute the regulation of CPO expression.
We have additionally isolated a novel gene, b819 gene that is restrictedly expressed in the blood island. This gene has no homology to any known protein, but primary structure presents a possible membrane protein. Knockdown analysis of b819 gene induced the suppression of blood cells production and the disorganization of network formation of blood vessels. Since both blood and endothelial cells are produced from the common precursor "hemangioblast", b819 gene may be a critical regulator in this process. Further studies will be required to reveal how b819 gene contributes the development of blood and endothelial cells during early embryogenesis. -
Functional analysis of a novel gene arf in the brain development during early zebrafish embryogenesis
Grant number：16570172 2004 - 2005
Japan Society for the Promotion of Science Kyoto University Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C) Grant-in-Aid for Scientific Research (C)
HANAOKA Ryuki, KAWAHARA Atsuo
We have isolated a novel factor arf (anterior brain regulating factor) that is specifically expressed in the anterior brain during early zebrafish embryogenesis. In this study, we have performed the functional analysis of arf. At first, we examined the expression pattern of arf by the whole-mount in situ hybridization analysis. (1) We found that arf is expressed in the anterior ectoderm (future anterior brain) during gastrulation stages and expressed in the anterior brain (forebrain and midbrain) and the formed somites during somitogenesis stages. Next, we investigated the knockdown analysis of the arf using anti-sense morpholinos for the arf gene (arf-MO). (2) Injection of arf-MO in the blastomere of the one-cell stage embryo induced the disorganization of anterior brain and the curly tail defects. These abnormalities are well correlated with the arf expression domains. These results suggest that arf plays an important role in the formation of brain and trunk including somites. We examined whether apoptotic cell death is affected in the arf-MO-injected embryos. (3) TUNEL assay showed that apoptotic cell death is broadly induced including both brain and trunk in the arf-MO-injected embryos. We finally examined the effect of arf overexpression. (4)We could not observe any morphological effect in the arf RNA-injected embryos. We should check the dose of arf RNA and the effect on the marker genes expression. These results suggest that arf is involved in the brain and trunk formation. Further studies will be required to elucidate how arf contributes the formation of anterior brain in early embryogenesis.
Papers
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Efficient detection of single nucleotide variants in targeted genomic loci Reviewed
Sone R, Fujimaki S, Kawahara A
Development Growth & Differentiation 2024.2
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Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis Reviewed
Suzuki H, Ogawa T, Fujita S, Sone R, Kawahara A
Scientific Reports 13 12279 2023.8
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Involvement of the zebrafish trrap gene in craniofacial development Reviewed Major achievement
Taichi Suzuki, Yo Hirai, Tomoko Uehara, Rie Ohga, Kenjiro Kosaki, Atsuo Kawahara
Scientific Reports 11 24166 2021.12( ISSN：2045-2322 )
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Critical roles of the ddx5 gene in zebrafish sex differentiation and oocyte maturation Reviewed
Sone R, Taimatsu K, Ohga R, Nishimura T, Tabaka M, Kawahara A
Scientific Reports 10 ( 1 ) 14157 2020.9( ISSN：2045-2322 )
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Involvement of the centrosomal protein 55 (cep55) gene in zebrafish head formation Reviewed
GENES TO CELLS 24 ( 10 ) 642 - 649 2019.10( ISSN：1356-9597 )
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Characterization of biklf/klf17-deficient zebrafish in posterior lateral line neuromast and hatching gland development Reviewed
Scientific Reports 9 ( 1 ) 13680 2019.9( ISSN：2045-2322 )
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Developmental expression of the slurp-like1/ly2.3/ly97.3 and slurp-like2/ly2.2/ly97.2 genes during zebrafish early embryogenesis Reviewed
Kawahara A, Morita H, Yanagi K, Ishizaka T, Taimatsu K, Ohga R
GENE EXPRESSION PATTERNS 30 32 - 36 2018.12( ISSN：1567-133X )
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Spatiotemporal expression of the cocaine- and amphetamine-regulated transcript-like (cart-like) gene during zebrafish embryogenesis Reviewed
Kawahara A, Morita H, Yanagi K, Suzuki H, Mori T, Ohga R, Taimatsu K
GENE EXPRESSION PATTERNS 30 1 - 6 2018.12( ISSN：1567-133X )
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Functional visualization and disruption of targeted genes using CRISPR/Cas9-mediated eGFP reporter integration in zebrafish Reviewed Major achievement
Ota S, Taimatsu K, Yanagi K, Namiki T, Ohga R, Higasijima S, Kawahara A
Scientific Reports 6 2016.9( ISSN：2045-2322 )
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Comprehensive analysis of sphingosine-1-phosphate receptor mutants during zebrafish embryogenesis Reviewed Major achievement
GENES TO CELLS 20 647 - 658 2015.6( ISSN：1356-9597 )
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Maternal and zygotic sphingosine kinase 2 are indispensable for cardiac development in zebrafish Reviewed Major achievement
JOURNAL OF BIOLOGICAL CHEMISTRY 290 14841 - 14851 2015.6( ISSN：0021-9258 )
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Efficient multiple genome modifications by the tracrRNA, crRNA and Cas9 protein complex in zebrafish Reviewed Major achievement
PLoS One 10 2015.4( ISSN：1932-6203 )
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Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish Reviewed Major achievement
Scientific Reports 5 2015.3( ISSN：2045-2322 )
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Multiple genome modifications by the CRISPR/Cas9 system in zebrafish Reviewed Major achievement
GENES TO CELLS 19 555 - 564 2014.7( ISSN：1356-9597 )
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Zebrafish: A model vertebrate suitable for the analysis of human genetic disorders Reviewed Major achievement
Congenital anomalities 54 8 - 11 2014.2
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Quantitative assay for TALEN activity at endogenous genomic loci Reviewed
Hisano Y, Ota S, Arakawa K, Muraki M, Kono N, Oshita K, Sakuma T, Tomita M, Yamamoto T, Okada Y, Kawahara A
Biology Open 2 363 - 367 2013.11( ISSN：2046-6390 )
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Genome editing using artificial site-specific nucleases in zebrafish Reviewed Major achievement
DEVELOPMENT GROWTH & DIFFERENTIATION 56 26 - 33 2013.9( ISSN：0012-1592 )
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Functional cooperation of spns2 and fibronectin in cardiac and lower jaw development Reviewed
Hisano Y, Ota S, Takada S, Kawahara A
Biology Open 2 ( 8 ) 789 - 794 2013.6( ISSN：2046-6390 )
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Efficient identification of TALEN-mediated genome modifications using heteroduplex mobility assays Reviewed
Ota S, Hisano Y, Muraki M, Hoshijima K, Dahlem TJ, Grunwald DJ, Okada Y, Kawahara A
GENES TO CELLS 18 ( 6 ) 450 - 458 2013.6( ISSN：1356-9597 )
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The functional roles of sphingosine-1-phosphate (S1P) in immunity Reviewed
Hisano Y, Nishi T, Kawahara A
JOURNAL OF BIOCHEMISTRY 152 ( 4 ) 305 - 311 2012.10( ISSN：0021-924X )
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Vap (Vascular Associated Protein): a novel factor involved in erythropoiesis and angiogenesis Reviewed
Kawahara A, Endo S, Dawid IB
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 421 ( 2 ) 367 - 374 2012.5( ISSN：0006-291X )
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Noncanonical activity of seryl-tRNA synthetase and vascular development Reviewed
Kawahara A, Stainier DYR
Trends in Cardiovascular Medicine 2009.8( ISSN：1050-1738 )
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Non-canonical activity of Seryl-tRNA synthetase is involved in vascular development Reviewed
Fukui H, Hanaoka R, Kawahara A
Circulation Research 104 ( 11 ) 1253 - 1259 2009.6( ISSN：0009-7330 )
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Transcriptional regulation of a myeloid-lineage specific gene lysozyme C during zebrafish myelopoiesis Reviewed
Kitaguchi T, Kawakami K, Kawahara A
Mechanisms of Development 126 314 - 323 2009.5( ISSN：0925-4773 )
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The sphingolipid transporter Spns2 functions in migration of zebrafish myocardial precursors Reviewed
Kawahara A, Nishi T, Hisano Y, Fukui H, Yamaguchi A, Mochizuki N
SCIENCE 323 524 - 527 2009.1( ISSN：0036-8075 )
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Characterization of the heme synthesis enzyme coproporphyrinogen oxidase (CPO) in zebrafish erythrogenesis Reviewed
Hanaoka R, Katayama S, Dawid IB, Kawahara A
Genes to Cells 11 ( 3 ) 293 - 303 2006.3( ISSN：1365-2443 )
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Zebrafish GADD45b genes are involved in somite development Reviewed
Kawahara A, Che YS, Hanaoka R, Takeda H, Dawid IB
Proc. Natl. Acad. Sci. USA 102 ( 2 ) 361 - 366 2005.1( ISSN：0027-8424 )
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Developmental expression of zebrafish emx1 during early embryogenesis Reviewed
Kawahara A, Dawid IB
Gene Expression Patterns 2 201 - 206 2002.11( ISSN：1567-133X )
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The homeobox gene mbx is involved in eye and tectum development Reviewed
Kawahara A, Chein CB, Dawid IB
Developmental Biology 248 ( 1 ) 107 - 117 2002.8( ISSN：0012-1606 )
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Critical role of biklf in erythroid cell differentiation in zebrafish Reviewed
Kawahara A, Dawid IB
Current Biology 11 ( 17 ) 1353 - 1357 2001.9( ISSN：0960-9822 )
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Antagonistic role of vega1 and bozozok/dharma homeobox genes in organizer formation Reviewed
Kawahara A, Whilm T, Solnica-Krezel L, Dawid IB
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 97 ( 22 ) 12121 - 12126 2000.10( ISSN：0027-8424 )
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Expression of the Krüppel-like zinc finger gene biklf during zebrafish development
Kawahara A, Dawid IB
MECHANISMS OF DEVELOPMENT 97 173 - 176 2000.10( ISSN：0925-4773 )
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Functional interaction of vega2 and goosecoid homeobox genes in zebrafish Reviewed
Kawahara A, Whilm T, Solnica-Krezel L, Dawid IB
Genesis 28 ( 2 ) 58 - 67 2000.10( ISSN：1526-968X )
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Caspase-independent cell killing by Fas-associated protein with death domain Reviewed
Kawahara A, Ohsawa Y, Matsumura H, Uchiyama Y, Nagata S
J. Cell Biol. 143 ( 5 ) 1353 - 1360 1998.11( ISSN：0021-9525 )
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Inhibition of Fas-induced apoptosis by Bcl-2 Reviewed
Kawahara A, Kobayashi T, Nagata S
Oncogene 17 ( 20 ) 2549 - 2554 1998.11( ISSN：1476-5594 )
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Fas-induced DNA fragmentation and proteolysis of nuclear protein Reviewed
Kawahara A, Enari M, Talanian RV, Wong WW, Nagata S
GENES TO CELLS 3 ( 5 ) 297 - 306 1998.5( ISSN：1356-9597 )
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Critical role of the interleukin 2 (IL-2) receptor g chain-associated Jak3 in IL-2-induced c-fos and c-myc, but not bcl-2 gene induction
Kawahara A, Minami Y, Miyazaki T, Ihle J.N, Taniguchi T
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 92 ( 19 ) 8724 - 8728 1995.9( ISSN：0027-8424 )
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Functional activation of Jak1 and Jak3 by selective association with IL-2 receptor subunits Reviewed
Miyazaki T, Kawahara A, Fujii H, Nakagawa Y, Minami Y, Liu ZJ, Oishi I., Silvennoinen O., Witthuhn B.A., Ihle J.N., Taniguchi T
Science 266 ( 5187 ) 1045 - 1047 1994.11( ISSN：0036-8075 )
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Evidence for the critical role of the cytoplasmic region of the IL-2 receptor g chain in IL-2, IL-4 and IL-7 signalling Reviewed
Kawahara A, Minami Y, Taniguchi T
Mol. Cell. Biol. 14 ( 8 ) 5433 - 5440 1994.8( ISSN：0270-7306 )
Books and Other Publications
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遺伝子の機能阻害
川原敦雄（ Role： Joint Work）
朝倉書店 2020.11 （ ISBN:978-4-254-17173-0 ）
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ゼブラフィッシュ実験ガイド
川原敦雄（ Role： Joint Work）
朝倉書店 2020.11
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小型魚類でのゲノム編集
川原敦雄、星島一幸（ Role： Joint Work）
羊土社 2019.12 （ ISBN:978-4-7581-2244-3 ）
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白血球循環と組織への遊走
（ Role： Joint Translation ）
エルゼビア・ジャパン株式会社 2018.3 （ ISBN:978-4-86034-917-2 ）
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分子細胞免疫学
（ Role： Joint Translation ）
エルゼビア・ジャパン株式会社 2018.3
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CRISPR/Cas9-mediated targeted knock-in of exogenous reporter genes in zebrafish Major achievement
Atsuo Kawahara（ Role： Contributor）
Humana Press 2017.6 （ ISBN:978-1-4939-7127-5 ）
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genome editing in animals
Atsuo Kawahara（ Role： Contributor）
Humana Press 2017.6
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小型魚類でのゲノム編集の利用
泰松清人、川原敦雄（ Role： Contributor）
裳華房 2016.12 （ ISBN:978-4-7853-5866-2 ）
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ゼブラフィッシュでのゲノム編集 Major achievement
川原敦雄、東島眞一（ Role： Contributor）
羊土社 2016.12 （ ISBN:978-4-7581-0359-6 ）
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All aboutゲノム編集
川原敦雄, 東島眞一（ Role： Contributor）
羊土社 2016.12
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ゲノム編集入門 : ZFN・TALEN・CRISPR-Cas9
泰松清人, 川原敦雄（ Role： Contributor小型魚類でのゲノム編集の利用）
裳華房 2016.12 （ ISBN:9784785358662 ）
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Detection of multiple genome modifications induced by the CRISPR/Cas9 system Major achievement
Satoshi Ota, Atsuo Kawahara（ Role： Contributor）
Humana Press 2016.7 （ ISBN:978-1-4939-3769-1 ）
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Zebrafish
Satoshi Ota, Atsuo Kawahara（ Role： ContributorDetection of multiple genome modifications induced by the CRISPR/Cas9 system）
Humana Press 2016.7
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小型魚類でのゲノム編集 Major achievement
川原敦雄、木下政人（ Role： Contributor-）
羊土社 2015.12 （ ISBN:978-4-7581-0193-6 ）
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ゲノム編集成功の秘訣Q&A : 論文だけではわからない : TALEN、CRISPR/Cas9の極意
川原敦雄, 木下政人（ Role： Contributor小型魚類でのゲノム編集）
羊土社 2015.12 （ ISBN:9784758101936 ）
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Sphingosine 1-phosphate signaling via transporters in zebrafish and mice Major achievement
Hisano Y, Nishi T, Kawahara A（ Role： Contributor）
Springer 2015.8
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Genome editing in zebrafish and medaka Major achievement
Kawahara A, Yabe T, Ansai S, Takada S, Kinoshita M（ Role： Contributor）
Springer 2015.8
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Targeted genome editing using site-specific nucleases
Atsuo Kawahara, Taijiro Yabe, Satoshi Ansai, Shinji Takada, Masato Kinoshita（ Role： ContributorGenome editing in zebrafish and medaka）
Springer 2015.8
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体が形づくられる不思議
（ Role： Contributor）
武庫川女子大学出版部 2015.4 （ ISBN:978-4-907594-01-5 ）
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命を守る生体の機構と科学
川原敦雄（ Role： Contributor体が形づくられる不思議）
武庫川女子大学出版部 2015.4 （ ISBN:9784907594015 ）
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ゼブラフィッシュにおけるゲノム編集と生命科学への応用 Major achievement
太田聡、川原敦雄（ Role： Contributor-）
羊土社 2014.7
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小型魚類におけるTALENおよびCRISPR/Cas9を用いた遺伝子改変
（ Role： Contributor）
羊土社 2014.4 （ ISBN:978-4-7581-0190-5 ）
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スフィンゴシン-1-リン酸の分泌機構と生理機能 Major achievement
久野悠、川原敦雄（ Role： Contributor）
医歯薬出版株式会社 2014.3
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スフィンゴシン-1-リン酸の分泌機構と生理機能
久野悠, 川原敦雄（ Role： Contributor）
医歯薬出版株式会社 2014.3
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ゼブラフィッシュを用いた脂質メディエーター研究
（ Role： Contributor）
メディカルドゥ 2013.5 （ ISBN:9784944157549 ）
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TALENによる遺伝子改変ゼブラフィッシュの作製
（ Role： Contributor）
秀潤社 2013.4 （ ISBN:978-4-7809-0142-9 ）
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Functional and physiological roles of sphingosine-1-phosphate transporter
（ Role： Contributor）
Wiley 2013.2 （ ISBN:978-0-470-56905-4 ）
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Bioactive lipid mediator
（ Role： ContributorSphingosine-1-phosphate signaling via transporters in zebrafish and mice）
Wiley 2013.2
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Lysophospholipid receptors : signaling and biochemistry
Chun, Jerold（ Role： Contributor）
Wiley 2013 （ ISBN:9780470569054 ）
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循環器系および免疫系を制御するスフィンゴシン-1-リン酸
川原敦雄（ Role： Single Work）
医学書院 2012.2
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スフィンゴシン-1-リン酸の輸送体
ニューサイエンス社 2010.12
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IL-2シグナル伝達
川原敦雄、谷口維紹（ Role： Joint Work）
中外医学社 1994.8 （ ISBN:4-498-00890-1 ）
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癌化のシグナル伝達機構
川原敦雄, 谷口維紹（ Role： Contributor）
中外医学社 1994.8
Presentations
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ヒト疾患に対する疾患モデル・ゼブラフィッシュの作製と機能解析
川原敦雄
第9回ゼブラフィッシュ・メダカ創薬研究会 2023.11
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Cooperative contributions of the klf1 and klf17 genes in zebrafish primitive erythropoiesis
Hiroaki Suzuki, Atsuo Kawahara
2023.9
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Efficient detection of small genetic mutations in the targeted genomic locus
Ryota Sone, Atsuo Kawahara
2023.9
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新規脂質メディエーターが制御する多彩な機能 分泌輸送体及び受容体を介したS1Pシグナリングの生理的役割の解析
久野 悠, 太田 聡, 村木 倫子, 川原 敦雄
日本生化学会大会プログラム・講演要旨集 2012.12 (公社)日本生化学会
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生命応答を制御する脂質マシナリー スフィンゴシン-1-リン酸(S1P)の初期発生における役割
川原 敦雄
日本生化学会大会・日本分子生物学会年会合同大会講演要旨集 2010.12 (公社)日本生化学会
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生理活性リゾリン脂質研究update Spns2はスフィンゴシン-1-リン酸(S1P)の細胞外への放出を制御する
川原 敦雄, 西 毅, 久野 悠, 山口 明人, 望月 直樹
日本生化学会大会プログラム・講演要旨集 2009.9 (公社)日本生化学会
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モデル動物を使った血管・リンパ管発生メカニズム 順遺伝学を用いたゼブラフィッシュの心臓・血管細胞の発生機構の解析
川原 敦雄
Inflammation and Regeneration 2008.7 (一社)日本炎症・再生医学会
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ボディーパターン形成を司るシグナル分子 新規ホメオボックス遺伝子vegaの体軸形成における役割
川原 敦雄
生化学 2000.8 (公社)日本生化学会
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IL-2のシグナル伝達とチロシンキナーゼ
川原 敦雄
生化学 1991.8 (公社)日本生化学会
Industrial Property Rights
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遺伝子改変型ゼブラフィッシュ
川原敦雄
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ヒト肥大型心筋症モデルゼブラフィッシュ及びその製造法、並びにヒト肥大型心筋症モデルゼブラフィッシュの利用
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Klf17 遺伝子が欠損した魚類乃至両生類、その製造方法、及び魚類乃至両生類の繁殖制御方法
Atsuo Kawahara
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スフィンゴシン1-リン酸の新規トランスポーター分子
望月直樹, 川原敦雄, 西毅, 山口明人
Teaching Experience (On-campus)
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生命科学実習
2024Year Type of subject：Professional education (undergraduate)
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生命科学
2024Year Type of subject：Professional education (undergraduate)
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人体の生命科学
2024Year Type of subject：Common education (undergraduate)
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ヒトの遺伝学
2024Year Type of subject：Professional education (undergraduate)
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病態・生理論
2024Year Type of subject：Professional education (undergraduate)
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循環器生物医学特論
2024Year Type of subject：Dr. (Graduate School)
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発生遺伝学特論
2024Year Type of subject：Dr. (Graduate School)
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生命科学特論II
2024Year Type of subject：Master's (Graduate School)
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Advanced course of circulation system Major achievement
2023Year
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Life Science II (Basic Medical Sciences: Host Defense and Pathological Regulation)
2023Year
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Practice in Life Sciences Major achievement
2023Year
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Life Science of the Human Body
2023Year
Guidance results
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2024
Type：Ph.D. dissertations guidance
Number of people receiving guidance ：1people
Number of teachers：1people
Other undergraduate student guidance
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2024
Class teacher - period： 2015
Social Activities
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ゲノム編集技術が生命科学研究と社会生活に与える衝撃
Role(s)： Lecturer, Demonstrator
2024.8
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ゲノムを自在に操れるゲノム編集技術ー科学が社会に与える影響ー
Role(s)： Lecturer
2017.7
Professional Memberships
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日本発生生物学会
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日本分子生物学会
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日本ゲノム編集学会
Media Coverage
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魚類の重要な遺伝子発見 生殖器官形成に影響 Newspaper, magazine
山梨日日新聞 2020.9
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遺伝子改変 孵化を阻止 Newspaper, magazine
朝日新聞 2019.9
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遺伝子「編集」熱帯魚で研究 Newspaper, magazine
朝日新聞 2015.3
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ゲノム領域 挿入効率化
日刊工業新聞 2015.3
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ゲノム編集に新技術 Newspaper, magazine
山梨日日新聞 2015.3