Language：English   Publishing type：Research paper (scientific journal)  

In human identification methods that target short tandem repeats (STRs), massively parallel sequencing (MPS) technology has made it possible to genotype at the level of the specific sequence itself. This allows for the detection of repeat unit variants and single nucleotide polymorphisms (SNPs) adjacent to the STRs. Using the GlobalFiler™ NGS STR Panel v2, Ion S5, and Converge software, this study constructed a Japanese database of 31 autosomal STRs (auSTRs) and two sex markers from 322 individuals. After excluding some sequence errors and stutters, a total of 31 novel alleles were identified. Additionally, using the allele frequencies of 31 auSTR loci, the match probabilities for the length-based and sequence-based data were calculated to be 1.433 × 10-34 and 9.163 × 10-38, respectively. These values are at least nine orders of magnitude higher than that obtained from 21 auSTR loci in the Japanese population using the conventional capillary electrophoresis method. The database generated in this study is expected to be implemented in forensic practice and used to solve difficult casework.

DOI： 10.1016/j.legalmed.2022.102151

PubMed

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.legalmed.2022.102136

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/gbe/evab192.

Language：English   Publishing type：Research paper (scientific journal)  

We present a case of fatal poisoning by 4-F-methcathinone (4-FMC; also called flephedrone), 4-methoxy-α-pyrrolidinopentiophenone (4-MeO-α-PVP), 4-fluoro-α-pyrrolidinopentiophenone (4-F-α-PVP), and α-pyrrolidinohepatanophenone (PV8). In this study, we compared the mass spectra of 4-FMC, 4-MeO-α-PVP, 4-F-α-PVP, PV8, and α-pyrrolidinohexanophenone between LC-ESI-LIT-MS and GC-EI-MS analyses. Subsequently, we applied LC-ESI-LIT-MS for detection and quantification analyses of 4-FMC, 4-MeO-α-PVP, 4-F-α-PVP, and PV8 in human authentic whole blood samples. More specific mass spectra for the target compounds were obtained with the LC-ESI-LIT-MS qualitative analyses than with the GC-EI-MS analyses, indicating that LC-ESI-LIT-MS was more suitable for the qualitative analysis of cathinones. The LC-ESI-LIT-MS validation data showed moderately good linearity and reproducibility for the compounds in the quantitative analyses at the range of 1-500 ng/mL. The detection limits of four cathinones ranged from 0.1 to 1 ng/mL. The concentrations of 4-FMC, 4-MeO-α-PVP, 4-F-α-PVP, and PV8 in heart whole blood samples were 365, 449, 145, and 218 ng/mL, respectively. Those of the 4 cathinones in femoral vein whole blood samples were 397, 383, 127, and 167 ng/mL, respectively. We can then assume that the cause of death was acute poisoning by a combination of 4-FMC, 4-MeO-α-PVP, 4-F-α-PVP, and PV8. In this article, we present a detailed LC-ESI-LIT-MS procedure for detection and quantification analyses of 4-FMC, 4-MeO-α-PVP, 4-F-α-PVP, and PV8 in authentic human whole blood samples.

DOI： 10.1016/j.forsciint.2021.110888

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s00371-021-02201-7

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1537/ase.2012132

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Anthropological Society of Nippon  

We examined fragmentary human skeletal remains from Escalon Cave near Surigao City, northeastern Mindanao, the Philippines, with respect to the morphology of bones and teeth, radiocarbon dating, and mitochondrial DNA (mtDNA) haplogroup. These remains contained parts of the left temporal bone, the right humerus, the right femur, the upper right first and second premolars, and the first molar. They are presumed to belong to an early-middle adult male, whom we named the Escalon Man. Using the femur sample, we estimated the14C date of the individual at 2692 ± 39 years BP (uncalibrated). His stature, estimated from the maximum femur length, was about 170 cm—this makes him much taller than the Aeta and the Mamanwa, who are indigenous negrito hunter-gatherers of the Philippines. The femur is sturdy and shows well-developed muscle markings. Numerous narrow grooves on the humerus were found in close proximity to each other, but we could not prove whether these are artificial cut marks made by humans with sharp-edged tools or scratches from some non-human entity. The mesiodistal crown diameters of the molar and premolars are large and resemble those of native Oceanians. Analysis of mtDNA haplogroup was carried out using a DNA sample extracted from a molar. A next-generation sequencer was used to determine the nucleotide sequences of the mtDNA genome. The results indicated that the Escalon Man belongs to the haplogroup E1a1a, which is known to be the marker of Austronesian-speaking agriculturist populations that originated in Taiwan and spread southwards through the Philippines to the Western Pacific, since about 4000 BP. Thus, the Escalon Man was likely a member of the late Neolithic or early Metal Age agriculturist peoples who settled in northeastern Mindanao and who may be the ancestors of the territory’s present-day occupants, such as the Manobo.

DOI： 10.1537/ase.200706

Scopus

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Internal Medicine  

Spastic paraplegia type 4 (SPG4) is caused by mutations of the SPAST gene and is the most common form of autosomal-dominantly inherited pure hereditary spastic paraplegia (HSP). We herein report a Japanese patient with SPG4 with a confirmed de novo mutation of SPAST. On exome sequencing and Sanger sequencing, we identified the heterozygous missense mutation p.R460L in the SPAST gene. This mutation was absent in the parents, and the paternity and maternity of the parents were both confirmed. The patient showed a pure SPG4 phenotype with an infantile onset. This study may expand the clinical and genetic findings for SPG4.

DOI： 10.2169/internalmedicine.4599-20

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Anthropological Society of Nippon  

DOI： 10.1537/ase.190415

Authorship：Last author   Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.1537/asj.1904231.

Authorship：Last author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Anthropological Society of Nippon  

© 2019 The Anthropological Society of Nippon. Recent studies have revealed that the Jomon people are considerably genetically different from any other population, including modern-day Japanese. This gives rise to an intriguing question: when after the Jomon era did this drastic change of genetic features occur? The Shomyoji shell midden site in Kanagawa, Japan can provide some clues to address this question. The skeletons buried at this site include some that are more recent than the Jomon-era skeletons with whom they are almost contiguous-ly buried. We tested the genetic continuity of the Shomyoji shell midden people by analyzing mitochondrial DNA (mtDNA). Our results show that the mtDNA haplogroups of the Kofun and Heian skeletons vastly differ from those of the Jomon skeletons. This finding implies that the genetic conversion of the Japanese people may have occurred during or before the Kofun era, at least at the Shomyoji site. To confirm this hypothesis, nuclear genome analysis of the Shomyoji people is considered promising.

DOI： 10.1537/ase.190307

Scopus

Language：English   Publishing type：Research paper (scientific journal)  

PURPOSE: Mepirapim is a new synthetic cannabinoid. We previously reported that the concentrations of unchanged mepirapim in whole blood and urine were much higher than those of other synthetic cannabinoids. To determine the postmortem distribution of mepirapim and acetyl fentanyl in the deceased individual, we established a standard addition method for detailed analysis by liquid chromatography-mass spectrometry (LC-MS) for quantification of these drugs. METHODS: The LC-MS method was fully validated for linearity, extraction recovery, matrix effect and repeatability. RESULTS: Good linearities, extraction recoveries, matrix effects and repeatabilities were shown for both target compounds in all specimens. The concentrations of mepirapim and acetyl fentanyl in three body fluid specimens and 12 solid tissue specimens were measured. For mepirapim, the highest concentrations were found in the liver and kidney, and the concentrations in the blood and urine specimens were one order of magnitude lower than the high concentrations in the solid tissues except the psoas major muscle. For acetyl fentanyl, the highest concentrations were found in the myocardium, spleen and kidney, and the concentrations in the body fluid specimens were also one order of magnitude lower than the highest concentrations in the solid tissues. There were concentration differences of mepirapim and acetyl fentanyl among the regions of the brain. CONCLUSIONS: The concentration of unchanged mepirapim in urine was much higher than those of other synthetic cannabinoids; the higher dosage, urinary excretion, metabolisms and/or pharmacokinetics of mepirapim may be quite different from those of other synthetic cannabinoids.

DOI： 10.1007/s11419-018-0431-z.

PubMed

Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (commerce magazine)   Publisher：雄山閣  

Language：English   Publishing type：Research paper (scientific journal)  

The Austronesian language is spread from Madagascar in the west, Island Southeast Asia (ISEA) in the east (e.g. the Philippines and Indonesian archipelagoes) and throughout the Pacific, as far east as Easter Island. While it seems clear that the remote ancestors of Austronesian speakers originated in Southern China, and migrated to Taiwan with the development of rice farming by c. 5500 BP and onto the northern Philippines by c. 4000 BP (the Austronesian Dispersal Hypothesis or ADH), we know very little about the origins and emergence of Austronesian speakers in the Indonesian Archipelago. Using a combination of cranial morphometric and ancient mtDNA analyses on a new dataset from Gua Hairmau, that spans the pre-Neolithic through to Metal Period (5712-5591cal BP to 1864-1719 cal BP), we rigorously test the validity of the ADH in ISEA. A morphometric analysis of 23 adult male crania, using 16 of Martin's standard measurements, was carried out with results compared to an East and Southeast Asian dataset of 30 sample populations spanning the Late Pleistocene through to Metal Period, in addition to 39 modern samples from East and Southeast Asia, near Oceania and Australia. Further, 20 samples were analyzed for ancient mtDNA and assigned to identified haplogroups. We demonstrate that the archaeological human remains from Gua Harimau cave, Sumatra, Indonesia provide clear evidence for at least two (cranio-morphometrically defined) and perhaps even three (in the context of the ancient mtDNA results) distinct populations from two separate time periods. The results of these analyses provide substantive support for the ADH model in explaining the origins and population history of ISEA peoples.

DOI： 10.1371/journal.pone.0198689

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer  

Purpose: We encountered a curious case in which two male subjects self-administered mepirapim plus acetyl fentanyl by different routes, i.e., intravenously and by inhalation. We have thus established a detailed procedure for quantification of mepirapim and acetyl fentanyl in whole blood and urine specimens using gas chromatography (GC)–tandem mass spectrometry (MS/MS). Methods: The GC–MS/MS method was validated for linearity, extraction recovery, accuracy, and precision. Liquid chromatography–MS/MS was also used for identification of the target compounds. Results: Good linearity and reproducibility were achieved in the range of 20–1000 ng/g for both target compounds in both matrices. The concentrations of mepirapim in heart whole blood, femoral vein whole blood, and urine of the deceased individual with administration by intravenous injection were 593, 567, and 527 ng/g, respectively
those of acetyl fentanyl were 155, 125, and 126 ng/g, respectively. The mepirapim and acetyl fentanyl concentrations in the urine specimen of the surviving individual who had administered them by inhalation were 4900 and 570 ng/g, respectively. Conclusions: To our knowledge, with the exception of a brief mention of a mepirapim concentration in a serum sample in emergency medicine, there are no reported data on the identification and quantification of mepirapim in biological samples. Mepirapim is a new synthetic cannabinoid. The concentration profiles of unchanged mepirapim in whole blood and urine were quite different and unique. A detailed clarification of such uniqueness is under way in our laboratory.

DOI： 10.1007/s11419-017-0384-7

Scopus

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley-Blackwell  

Objectives: The Ainu, the indigenous people living on the northernmost island of Japan, Hokkaido, have long been a focus of anthropological interest because of their cultural, linguistic, and physical identity. A major problem with genetic studies on the Ainu is that the previously published data stemmed almost exclusively from only 51 modern-day individuals living in Biratori Town, central Hokkaido. To clarify the actual genetic characteristics of the Ainu, individuals who are less influenced by mainland Japanese, who started large-scale immigration into Hokkaido about 150 years ago, should be examined. Moreover, the samples should be collected from all over Hokkaido. Materials and methods: Mitochondrial DNA haplogroups of 94 Ainu individuals from the Edo era were successfully determined by analyzing haplogroup-defining polymorphisms in the hypervariable and coding regions. Thereafter, their frequencies were compared to those of other populations. Results: Our findings indicate that the Ainu still retain the matrilineage of the Hokkaido Jomon people. However, the Siberian influence on this population is far greater than previously recognized. Moreover, the influence of mainland Japanese is evident, especially in the southwestern part of Hokkaido that is adjacent to Honshu, the main island of Japan. Discussion: Our results suggest that the Ainu were formed from the Hokkaido Jomon people, but subsequently underwent considerable admixture with adjacent populations. The present study strongly recommends revision of the widely accepted dual-structure model for the population history of the Japanese, in which the Ainu are assumed to be the direct descendants of the Jomon people.

DOI： 10.1002/ajpa.23338

Scopus

PubMed

Language：English   Publishing type：Research paper (international conference proceedings)   Publisher：Elsevier  

An archaeological site that has three cemeteries was found in northeastern part of Japan. A total of 45 human skeletons were excavated from the site and dated to about early 18th to mid-19th centuries based on the archaeological evidences. In the previous study, three skeletons were excluded from the results because the partial mtDNA sequences of these samples were identical to those of the two researchers who were in charge of skeletal reconstruction. In this study, the whole mtDNA sequences of these three skeletons and the two researchers were amplified by the Precision ID mtDNA Whole Genome Panel kit, followed by the massively parallel sequencing using the Ion PGM™ System (Thermo Fisher Scientific). The three skeleton samples had the same whole mtDNA sequences with 38 mutations as compared to the rCRS, being assigned to haplogroup D4a1c. These results suggested that the three skeletons might belong to the same maternal line. Meanwhile, the two researchers were typed as “D4a1*” and “D4a1b”. Although analyses of the hyper variable regions I–III and the conventional partial sequencing of mtDNA could not discriminate skeletons from the researchers, the mtDNA whole genome sequencing made it possible. Thus the whole mtDNA sequencing using the Ion PGM system is highly effective to shed light on the matrilineal relationship of the ancient human skeletons.

DOI： 10.1016/j.fsigss.2017.09.061

Scopus

Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Last author   Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：The Anthropological Society of Nippon  

<p>To investigate the genetic changes in the indigenous population of northern Kyushu Island from the early Jomon to Yayoi periods, mitochondrial DNA from nine human skeletal remains excavated from the Iwashita Cave and Shimo-motoyama rock shelter sites located in Sasebo city was examined using the amplified product length polymorphism (APLP) method and next-generation sequencing analyses. Although mitochondrial DNA could be extracted from two samples, we could not determine the genetic characteristics of the population in this area and their changes from the Jomon to Yayoi periods accurately. However, the average sequencing depths for these samples were 178.73 and 61.02, which indicates the validity of the DNA capture methods used in this study. The individuals who were successfully analyzed belonged to the northwestern Kyushu Yayoi population, which is thought to be a descendant of the Jomon people. Therefore, the current data are expected to be used to speculate the characteristics of the mitochondrial DNA of the Kyushu Jomon population. However, mitochondrial haplogroups were assigned as M7a and D4a1 by APLP analysis, and based on the whole mitochondrial DNA genome sequencing data, the ancestral types were found to be M7a1a4 and D4a1. Haplogroup M7a has been detected at several Jomon sites in Honshu and Hokkaido and is thought to be a typical Jomon haplogroup. On the other hand, the existence of haplogroup D4a, considered to be derived from immigrant Yayoi population at the site suggested that the segregation of the indigenous Jomon people and immigrants in this region was not clear.</p>

DOI： 10.1537/asj.170509

CiNii Books

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：PUBLIC LIBRARY SCIENCE  

Sex determination is important in archeology and anthropology for the study of past societies, cultures, and human activities. Sex determination is also one of the most important components of individual identification in criminal investigations. We developed a new method of sex determination by detecting a single-nucleotide polymorphism in the amelogenin gene using amplified product-length polymorphisms in combination with sex-determining region Y analysis. We particularly focused on the most common types of postmortem DNA damage in ancient and forensic samples: fragmentation and nucleotide modification resulting from deamination. Amplicon size was designed to be less than 60 bp to make the method more useful for analyzing degraded DNA samples. All DNA samples collected from eight Japanese individuals (four male, four female) were evaluated correctly using our method. The detection limit for accurate sex determination was determined to be 20 pg of DNA. We compared our new method with commercial short tandem repeat analysis kits using DNA samples artificially fragmented by ultraviolet irradiation. Our novel method was the most robust for highly fragmented DNA samples. To deal with allelic dropout resulting from deamination, we adopted "bidirectional analysis," which analyzed samples from both sense and antisense strands. This new method was applied to 14 Jomon individuals (3500-year-old bone samples) whose sex had been identified morphologically. We could correctly identify the sex of 11 out of 14 individuals. These results show that our method is reliable for the sex determination of highly degenerated samples.

DOI： 10.1371/journal.pone.0169348

Web of Science

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： DOI:10.1537/asj.0160417

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：PUBLIC LIBRARY SCIENCE  

Mitochondrial DNA (mtDNA) serves as a powerful tool for exploring matrilineal phylogeo-graphic ancestry, as well as for analyzing highly degraded samples, because of its polymorphic nature and high copy numbers per cell. The recent advent of complete mitochondrial genome sequencing has led to improved techniques for phylogenetic analyses based on mtDNA, and many multiplex genotyping methods have been developed for the hierarchical analysis of phylogenetically important mutations. However, few high-resolution multiplex genotyping systems for analyzing East-Asian mtDNA can be applied to extremely degraded samples. Here, we present a multiplex system for analyzing mitochondrial single nucleotide polymorphisms (mtSNPs), which relies on a novel amplified product-length polymorphisms (APLP) method that uses inosine-flapped primers and is specifically designed for the detailed haplogrouping of extremely degraded East-Asian mtDNAs. We used fourteen 6-plex polymerase chain reactions (PCRs) and subsequent electrophoresis to examine 81 haplogroup-defining SNPs and 3 insertion/deletion sites, and we were able to securely assign the studied mtDNAs to relevant haplogroups. Our system requires only 1x10(-13) g (100 fg) of crude DNA to obtain a full profile. Owing to its small amplicon size (&lt; 110 bp), this new APLP system was successfully applied to extremely degraded samples for which direct sequencing of hypervariable segments using mini-primer sets was unsuccessful, and proved to be more robust than conventional APLP analysis. Thus, our new APLP system is effective for retrieving reliable data from extremely degraded East-Asian mtDNAs.

DOI： 10.1371/journal.pone.0158463

Web of Science

Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.legalmed.2015.12.006

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1537/ase.016060

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.3919/jjsa.76.2724

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：PUBLIC LIBRARY SCIENCE  

Polymerase chain reaction-amplified product length polymorphism (PCR-APLP) is one of the most convenient and reliable methods for single nucleotide polymorphism (SNP) analysis. This method is based on PCR, but uses allele-specific primers containing SNP sites at the 3'-terminus of each primer. To use this method at least two allele-specific primers and one "counter-primer", which serves as a common forward or reverse primer of the allele-specific primers, are required. The allele-specific primers have SNP sites at the 30-terminus, and another primer should have a few non-complementary flaps at the 5'-terminus to detect SNPs by determining the difference of amplicon length by PCR and subsequent electrophoresis. A major disadvantage of the addition of a non-complementary flap is the non-specific annealing of the primer with non-complementary flaps. However, a design principle for avoiding this undesired annealing has not been fully established, therefore, it is often difficult to design effective APLP primers. Here, we report allele-specific primers with an inosine chain at the 5'-terminus for PCR-APLP analysis. This unique design improves the competitiveness of allele-specific primers and the reliability of SNP analysis when using the PCR-APLP method.

DOI： 10.1371/journal.pone.0136995

Web of Science

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s00414-014-1065-2

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.legalmed.2014.08.006

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.1537/asj.140905

Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER IRELAND LTD  

Mitochondrial DNA (mtDNA) is widely used for DNA analysis of highly degraded samples because of its polymorphic nature and high number of copies in a cell. However, as endogenous mtDNA in deteriorated samples is scarce and highly fragmented, it is not easy to obtain reliable data. In the current study, we report the risks of direct sequencing mtDNA in highly degraded material, and suggest a strategy to ensure the quality of sequencing data. It was observed that direct sequencing data of the hypervariable segment (HVS) I by using primer sets that generate an amplicon of 407 bp (long-primer sets) was different from results obtained by using newly designed primer sets that produce an amplicon of 120-139 bp (mini-primer sets). The data aligned with the results of mini-primer sets analysis in an amplicon length-dependent manner; the shorter the amplicon, the more evident the endogenous sequence became. Coding region analysis using multiplex amplified product-length polymorphisms revealed the incongruence of single nucleotide polymorphisms between the coding region and HVS 1 caused by contamination with exogenous mtDNA. Although the sequencing data obtained using long-primer sets turned out to be erroneous, it was unambiguous and reproducible. These findings suggest that PCR primers that produce amplicons shorter than those currently recognized should be used for mtDNA analysis in highly degraded samples. Haplogroup motif analysis of the coding region and HVS should also be performed to improve the reliability of forensic mtDNA data. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.legalmed.2013.10.001

Web of Science

Authorship：Lead author,　Last author,　Corresponding author   Language：English   Publishing type：Research paper (bulletin of university, research institution)  

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：ANTHROPOLOGICAL SOC NIPPON  

Obtaining genetic information about early humans is indispensable to our understanding of the demographic history of mankind. In the present study, we performed a detailed mitochondrial DNA (mtDNA) analysis of a skeleton of the initial Jomon era unearthed from the Yugura cave site in Nagano, Japan, which was dated to 7920-7795 calBP by direct C-14 dating. mtDNA of the Yugura skeleton was designated to haplogroup D4b2, which is widely observed in present-day East Asians, including the Japanese, but is absent in Hokkaido Jomon people. This finding indicates that the basal population of Japan was heterogeneous with respect to their mtDNA lineage. This is the first report on the genotype of the people from the initial phase of the Jomon period.

DOI： 10.1537/ase.130313

Web of Science

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.fsigen.2012.09.006

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.006672

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

To clarify the colonizing process of East/Northeast Asia as well as the peopling of the Americas, identifying the genetic characteristics of Paleolithic Siberians is indispensable. However, no genetic information on the Paleolithic Siberians has hitherto been reported. In the present study, we analyzed ancient DNA recovered from Jomon skeletons excavated from the northernmost island of Japan, Hokkaido, which was connected with southern Siberia in the Paleolithic period. Both the control and coding regions of their mitochondrial DNA (mtDNA) were analyzed in detail, and we confidently assigned 54 mtDNAs to relevant haplogroups. Haplogroups N9b, D4h2, G1b, and M7a were observed in these individuals, with N9b being the predominant one. The fact that all these haplogroups, except M7a, were observed with relatively high frequencies in the southeastern Siberians, but were absent in southeastern Asian populations, implies that most of the Hokkaido Jomon people were direct descendants of Paleolithic Siberians. The coalescence time of N9b (ca. 22,000 years) was before or during the last glacial maximum, implying that the initial trigger for the Jomon migration in Hokkaido was increased glaciations during this period. Interestingly, Hokkaido Jomons lack specific haplogroups that are prevailing in present-day native Siberians, implying that diffusion of these haplogroups in Siberia might have been after the beginning of the Jomon era, about 15,000 years before present.

DOI： 10.1002/ajpa.21561

PubMed

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1556-4029.2010.01579.x

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.neuroscience.2010.10.018

Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：岩波書店  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Ancient DNA recovered from 16 Jomon skeletons excavated from Funadomari site, Hokkaido, Japan was analyzed to elucidate the genealogy of the early settlers of the Japanese archipelago. Both the control and coding regions of their mitochondrial DNA were analyzed in detail, and we could securely assign 14 mtDNAs to relevant haplogroups. Haplogroups D1a, M7a, and N9b were observed in these individuals, and N9b was by far the most predominant. The fact that haplogroups N9b and M7a were observed in Hokkaido Jomons bore out the hypothesis that these haplogroups are the (pre-) Jomon contribution to the modern Japanese mtDNA pool. Moreover, the fact that Hokkaido Jomons shared haplogroup D1 with Native Americans validates the hypothesized genetic affinity of the Jomon people to Native Americans, providing direct evidence for the genetic relationships between these populations. However, probably due to the small sample size or close consanguinity among the members of the site, the frequencies of the haplogroups in Funadomari skeletons were quite different from any modern populations, including Hokkaido Ainu, who have been regarded as the direct descendant of the Hokkaido Jomon people. It appears that the genetic study of ancient populations in northern part of Japan brings important information to the understanding of human migration in northeast Asia and America.

DOI： 10.1002/ajpa.20923

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.forsciint.2008.10.003

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier  

DOI： 10.1016/j.legalmed.2007.01.007

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier  

DOI： 10.1016/j.legalmed.2006.11.002

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-LISS  

Ancient DNA recovered from 57 individuals excavated by Hiram Bingham at the rural communities of Paucarcancha, Patallacta, and Huata near the famed Inca royal estate and ritual site of Machu Picchu was analyzed by polymerase chain reaction, and the results were compared with ancient and modern DNA from various Central Andean areas to test their hypothesized indigenous highland origins. The control and coding regions of the mitochondrial DNA (mtDNA) of 35 individuals in this group were sequenced, and the haplogroups of each individual were determined. The frequency data for the haplogroups of these samples show clear proximity to those of modern Quechua and Aymara populations in the Peruvian and Bolivian highlands, and contrast with those of pre-Hispanic individuals of the north coast of Peru that we defined previously. Our study suggests a strong genetic affinity between sampled late pre-Hispanic individuals and modern Andean highlanders. A previous analysis of the Machu Picchu osteological collection suggests that the residents there were a mixed group of natives from various coastal and highland regions relocated by the Inca state for varied purposes. Overall, our study indicates that the sampled individuals from Paucarcancha and Patallacta were indigenous highlanders who provided supportive roles for nearby Machu Picchu. © 2006 Wiley-Liss, Inc.

DOI： 10.1002/ajpa.20408

Web of Science

Scopus

PubMed

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer  

DOI： 10.1111/j.1447-073X.2006.00122.x

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：ANTHROPOLOGICAL SOC NIPPON  

Kinship analysis was performed on a Jomon double burial from the Usu-Moshiri site in Hokkaido; Japan, using odontometric data in conjunction with mitochondrial DNA data. Q-mode correlations with respect to tooth crown measurements indicated a low similarity between the two adult female skeletons found from this burial. Moreover, mitochondrial DNA analysis revealed that these individuals were not maternal relatives. Consequently, judging from both morphological and genetic evidence. these skeletons are more likely to have been unrelated rather than consanguineous. This is the first report that provides anthropological evidence for a lack of kinship of skeletons discovered from a Jomon double burial.

DOI： 10.1537/ase.041228

Web of Science

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1002/elps.200406129

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jas.2004.02.011

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer  

DOI： 10.1007/s004140000150

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Total pages：378   Responsible for pages：214-216   Language：Japanese   Book type：Report

Total pages：378   Responsible for pages：214-216   Language：Japanese   Book type：Report

Responsible for pages：87 (6): 555-558.   Language：Japanese   Book type：Scholarly book

Language：English   Book type：Scholarly book

Language：English   Book type：Scholarly book

Responsible for pages：109-111   Language：Japanese   Book type：Report

Responsible for pages：406-417   Language：English   Book type：Scholarly book

Responsible for pages：406-417   Language：English   Book type：Scholarly book

Responsible for pages：109-111   Language：Japanese   Book type：Report

Responsible for pages：236-238   Language：Japanese   Book type：Textbook, survey, introduction

Responsible for pages：236-238   Language：Japanese   Book type：Textbook, survey, introduction

Total pages：305   Responsible for pages：130-133   Language：Japanese   Book type：Scholarly book

Total pages：305   Responsible for pages：130-133   Language：Japanese   Book type：Scholarly book

Total pages：3   Responsible for pages：50-52   Language：Japanese   Book type：Report

Total pages：3   Responsible for pages：50-52   Language：Japanese   Book type：Report

Total pages：181   Responsible for pages：95-104   Language：Japanese   Book type：Scholarly book

Total pages：181   Responsible for pages：95-104   Language：Japanese   Book type：Scholarly book

Responsible for pages：211-215   Language：Japanese   Book type：Scholarly book

Responsible for pages：211-215   Language：Japanese   Book type：Scholarly book

Total pages：215   Responsible for pages：10-21   Language：Japanese   Book type：Scholarly book

Total pages：215   Responsible for pages：10-21   Language：Japanese   Book type：Scholarly book

Total pages：5   Responsible for pages：265-269   Language：Japanese   Book type：Scholarly book

Total pages：5   Responsible for pages：265-269   Language：Japanese   Book type：Scholarly book

Total pages：4   Responsible for pages：287-290   Language：Japanese   Book type：Scholarly book

Total pages：4   Responsible for pages：287-290   Language：Japanese   Book type：Scholarly book

Responsible for pages：86-90   Language：Japanese   Book type：Scholarly book

Responsible for pages：83-85   Language：Japanese   Book type：Scholarly book

Responsible for pages：83-85   Language：Japanese   Book type：Scholarly book

Responsible for pages：86-90   Language：Japanese   Book type：Scholarly book

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Responsible for pages：242-245   Language：Japanese   Book type：Scholarly book

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Responsible for pages：124-128   Language：Japanese   Book type：Scholarly book

Responsible for pages：274-278   Language：Japanese   Book type：Scholarly book

Responsible for pages：124-128   Language：Japanese   Book type：Scholarly book

Responsible for pages：274-278   Language：Japanese   Book type：Scholarly book

Responsible for pages：276-279   Language：Japanese   Book type：Scholarly book

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Responsible for pages：168-170   Language：Japanese   Book type：Scholarly book

Responsible for pages：141-144   Language：Japanese   Book type：Scholarly book

Responsible for pages：141-144   Language：Japanese   Book type：Scholarly book

Responsible for pages：168-170   Language：Japanese   Book type：Scholarly book

Responsible for pages：933-936   Language：English   Book type：Scholarly book

Responsible for pages：727-730   Language：English   Book type：Scholarly book

Responsible for pages：724-726   Language：English   Book type：Scholarly book

Responsible for pages：743-745   Language：English   Book type：Scholarly book

Responsible for pages：743-745   Language：English   Book type：Scholarly book

Responsible for pages：933-936   Language：English   Book type：Scholarly book

Responsible for pages：724-726   Language：English   Book type：Scholarly book

Responsible for pages：727-730   Language：English   Book type：Scholarly book

Responsible for pages：275-278   Language：Japanese   Book type：Scholarly book

Responsible for pages：152-155   Language：Japanese   Book type：Scholarly book

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Responsible for pages：275-278   Language：Japanese   Book type：Scholarly book

Responsible for pages：188-191   Language：Japanese  

Responsible for pages：188-191   Language：Japanese  

Event date： 2022.9

Language：Japanese   Presentation type：Symposium workshop panel(public)  

Venue：京都府京都市　京都産業会館ホール  

Event date： 2022.6

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：ウインクあいち, 愛知県名古屋市.  

Event date： 2022.4

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：福岡県福岡市・オンデマンドウェブ開催(福岡国際会議場).  

Event date： 2022.1

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：ウェブ開催.  

Event date： 2021.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：石川県金沢市  

Event date： 2021.10

Language：Japanese   Presentation type：Symposium workshop panel(public)  

Venue：ウェブ開催  

Event date： 2020.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：シアター1010, 東京都葛飾区.  

Event date： 2020.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：名古屋市立大学医学研究棟講義室A, 愛知県名古屋市  

Event date： 2020.9

Language：English   Presentation type：Oral presentation(general)  

Event date： 2019.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：京都府京都市  

Event date： 2019.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：愛知県名古屋市  

Event date： 2019.10

Language：Japanese   Presentation type：Poster presentation  

Venue：佐賀県佐賀市  

Event date： 2019.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：佐賀県佐賀市  

Event date： 2019.6

Language：English   Presentation type：Oral presentation(general)  

Event date： 2019.6

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：東北学院大学ホーイ記念館地下ホール, 宮城県仙台市.  

Event date： 2019.3

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：朱鷺メッセ 新潟コンベンションセンター, 新潟県新潟市  

Event date： 2018.12

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：, 松江イングリッシュガーデン, 島根県松江市.  

Event date： 2018.10

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：国立遺伝学研究所, 静岡県三島市  

Event date： 2018.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：新潟大学医学部学士会有壬記念館, 新潟県新潟市  

Event date： 2018.5

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：明治大学リバティータワー，東京都文京区  

Event date： 2017.12

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：東京大学文学部, 東京都文京区  

Event date： 2017.12

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：大船渡市リアスホール，岩手県大船渡市  

Event date： 2017.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：岐阜大学医学部記念会館2階ホール，岐阜県岐阜市.  

Event date： 2017.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京大学理学部2号館2階221号室，東京都文京区.  

Event date： 2017.2

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：江陽グランドホテル，宮城県仙台市.  

Event date： 2017.1

Language：English   Presentation type：Symposium workshop panel(nominated)  

Venue：Gotland Campus, Uppsala University, Sweden.  

Event date： 2016.12

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京大学大気海洋研究所，千葉県柏市.  

Event date： 2016.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：福井大学医学部白翁会ホール，福井県福井市.  

Event date： 2016.10

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：新潟市民プラザ，新潟県新潟市.  

Event date： 2016.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：NGS学生総合プラザSTEP，新潟県新潟市  

Event date： 2016.9

Language：English   Presentation type：Symposium workshop panel(nominated)  

Venue：Kyoto, Japan.  

Event date： 2016.8

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：札幌国際ビル・国際ホール，北海道札幌市.  

Event date： 2016.7

Language：English   Presentation type：Poster presentation  

Venue：Hangzhou, China  

Event date： 2016.1

Language：English   Presentation type：Symposium workshop panel(nominated)  

Venue：Oxford, United Kingdom  

Event date： 2015.11

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：青森県むつ市・むつ来さまい館  

Event date： 2015.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：産業技術総合研究所臨海副都心センター，東京.  

Event date： 2015.6

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：高知市  

Event date： 2015.6

Language：English   Presentation type：Poster presentation  

Event date： 2014.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：名古屋  

Event date： 2014.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：浜松  

Event date： 2014.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：浜松  

Event date： 2014.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：藤田保健衛生大学  

Event date： 2014.6

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：福岡  

Event date： 2014.6

Language：English   Presentation type：Poster presentation  

Venue：Fukuoka  

Event date： 2014.6

Language：English   Presentation type：Poster presentation  

Venue：Fukuoka  

Event date： 2014.5

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：札幌  

Event date： 2014.5

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：首都大学東京南大沢キャンパス  

Event date： 2013.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：仙台市  

Event date： 2013.11

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：仙台市  

Event date： 2013.11

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：つくば・東京  

Event date： 2013.11

Language：Japanese   Presentation type：Oral presentation(general)  

Event date： 2013.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：つくば・東京  

Event date： 2013.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：金沢  

Event date： 2013.9

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：長野県佐久市  

Event date： 2013.4

Language：English   Presentation type：Symposium workshop panel(nominated)  

Venue：Honolulu, Hawaii, USA  

Event date： 2012.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：京都  

Event date： 2012.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：横浜  

Event date： 2012.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：松本  

Event date： 2012.2

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：甲府市  

Event date： 2012.1

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：甲府市  

Event date： 2011.12

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：横浜  

Event date： 2011.11

Language：English   Presentation type：Symposium workshop panel(nominated)  

Event date： 2011.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：愛知  

Event date： 2011.6

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：福島  

Event date： 2010.12

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：神戸  

Event date： 2010.12

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：甲府市  

Event date： 2010.11

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：岩手県立博物館  

Event date： 2010.10

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：北海道伊達市  

Event date： 2010.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：北海道伊達市  

Event date： 2010.9

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：札幌  

Event date： 2010.7

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：札幌  

Event date： 2010.5

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：札幌  

Event date： 2010.5

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京  

Event date： 2010.2

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：東京  

Event date： 2009.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京  

Event date： 2009.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京  

Event date： 2009.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：浜松  

Event date： 2009.5

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：千里ライフサイエンスセンター、大阪  

Event date： 2009.5

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：大阪、千里ライフサイエンスセンター  

Event date： 2008.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：東京  

Event date： 2008.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：名古屋  

Event date： 2008.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：金沢  

Event date： 2008.10

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：札幌  

Event date： 2007.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：大阪  

Event date： 2007.10

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：新潟  

Event date： 2007.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：横浜  

Event date： 2007.6

Language：English   Presentation type：Oral presentation(general)  

Venue：Okinawa  

Event date： 2007.5

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：秋田  

Event date： 2007.2

Language：English   Presentation type：Oral presentation(invited, special)  

Event date： 2006.11

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：高知  

Event date： 2006.3

Language：English   Presentation type：Oral presentation(general)  

Event date： 2005.10

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Venue：仙台  

Event date： 2004.4

Language：English   Presentation type：Oral presentation(general)  

Event date： 2002.9

Language：English   Presentation type：Oral presentation(general)  

Venue：Tokyo  

Event date： 2002.4

Language：English   Presentation type：Oral presentation(general)  

Event date： 1999.8

Language：English   Presentation type：Oral presentation(general)  

Event date： 1999.8

Language：English   Presentation type：Poster presentation  

Event date： 1998.7

Language：English   Presentation type：Poster presentation  

Venue：Kobe  

Language：English   Presentation type：Poster presentation  

Venue：Kobe  

Language：English   Presentation type：Poster presentation  

Venue：Kobe  

Language：English   Presentation type：Poster presentation  

Venue：Kobe  

Event date： 1996.8

Language：English   Presentation type：Poster presentation  

Venue：Tokyo  

法医解剖

法医解剖

山梨県児童相談所からの被虐待疑い事例の相談3件に対し、法医学的見地から損傷の鑑定および助言をおこなった。

山梨県児童相談所からの被虐待疑い事例の相談3件に対し、法医学的見地から損傷の鑑定および助言をおこなった。

Audience： General,　Media

Type：TV or radio program

Type：Other

相談件数9件の事例に対し、法医学的見地から助言した。

Type：Other

相談件数9件の事例に対し、法医学的見地から助言した。

東日本大震災に対する日本法医学会・災害時死体検案支援医師として、宮城県に於いて、震災による死者112人の死体検案をおこなった。