日本医療研究開発機構   「革新的技術による脳機能ネットワークの全容解明プロジェクト」   プログラムオフィサー(PO)

2015.9

日本神経科学学会　   日本神経科学学会　   副庶務理事

2020.1

日本神経科学学会　   日本神経科学学会　   庶務理事

2016.12 - 2019.12

文部科学省   研究振興局   学術調査官

2013.8 - 2015.7

University of Toyama   Associate Professor

2007.4 - 2009.3

University of Toyama

2006.4 - 2007.3

Toyama Medical and Pharmaceutical University

2005.4 - 2006.3

カン研究所　   グループリーダー

2000.4 - 2005.3

日本学術振興会特別研究員（PD）

1998.4 - 2000.3

日本学術振興会特別研究員（DC2）

1996.4 - 1998.3

臓器間ネットワークの変容を早期に検出可能な新規分子・バイオマーカーの探索とその応用によるリスク予見法の創出

Grant number：20338617  2021.4 - 2026.3

国立研究開発法人科学技術振興機構  臓器連関の包括的理解に基づく認知症関連疾患の克服に向けて  ムーンショット型研究開発事業

高橋良輔

２相性インスリン極性分泌機構とその破綻による糖尿病発症

Grant number：21H02431  2021.4 - 2024.3

日本学術振興会  基盤研究B  基盤研究B

今泉美佳

神経・内分泌モジュレーター分子の放出制御から捉える中枢ー末梢機能連関の解明

2020.6 - 2021.5

公益財団法人　三菱財団  自然科学研究助成（一般助成）  自然科学研究助成（一般助成）

Bassoon proteinopathyの病態解析研究

Grant number：20H03585  2020.4 - 2023.3

日本学術振興会  基盤研究B  基盤研究B

矢部一郎　

シナプス伝達効率を調節するアクティブゾーンの分子構造基盤と修飾機構

Grant number：19H03324   2019.4 - 2022.3

日本学術振興会  基盤研究（Ｂ）  補助金・基盤研究（Ｂ）

大塚稔久

シナプス伝達制御と中枢ー末梢機能連関から捉える意志動力学

Grant number：19H05014  2019.4 - 2021.3

日本学術振興会  新学術領域研究　研究領域提案型意思動力学  新学術領域研究　研究領域提案型意思動力学

大塚稔久

シナプス光遺伝学を用いた脳領域間シグナル伝播機構の解明

Grant number：JPMJCR1751   2017.10 - 2023.3

国立研究開発法人科学技術振興機構  戦略的創造研究推進事業　チーム型研究CREST  光の特性を活用した生命機能の時空間制御技術の開発と応用

礒村宜和

CAST/ELKSの機能制御から捉える網膜シナプスのスクラップアンドビルド

Grant number：17H05741  2017.4 - 2019.3

新学術領域研究（研究領域提案型）  補助金・新学術領域研究（研究領域提案型）スクラップアンドビルド

プレシナプス分子の動作原理から捉える適応回路メカニズム

Grant number：17H05560  2017.4 - 2019.3

新学術領域研究（研究領域提案型）  補助金・新学術領域研究（研究領域提案型）適応回路シフト

包括型脳科学研究推進支援ネットワーク　研究集会委員長

2015.5 - 2016.3

新学術領域研究

CAST/ELKS蛋白質ファミリーによるアクティブゾーン機能制御機構の解明

Grant number：15H04272  2015.4 - 2018.3

基盤研究B  基盤研究B

神経シナプスアクティブゾーンの分子構造基盤と生理機能の解明

Grant number：22300120  2010.4 - 2013.3

日本学術振興会  基盤研究B  基盤研究B

Critical role of the presynaptic protein CAST in maintaining the photoreceptor triad at ribbon synapses. Reviewed

Hagiwara A, Mizutani A, Kawamura S, Abe M, Hida Y, Martemyanov KA, Sakimura K, Ohtsuka T.*

International Journal of Molecular Sciences   24 ( 8 )   7251   2023.3

A novel technique for large-fragment knock-in animal production without ex vivo handling of zygotes Invited Reviewed

Abe M, NakatsukasaE, Natsume R, Hamada S, Sakimura K, Watabe AM, Ohtsuka, T.

Scientific Reports   13   2023.2( ISSN：2045-2322 )

Post-developmental plasticity of the primary rod pathway allows restoration of visually guided behaviors. Reviewed

Cao Y., Fajardo D., Guerrero-Given D., A.Samuel M., Ohtsuka T., Boye SE., Kamasawa N., Martemyanov KA.

CURRENT BIOLOGY   32 ( 22 )   4783 - 4796   2022.9( ISSN：0960-9822 )

Ubap1 knock-in mice reproduced the phenotype of SPG80 Reviewed

Shimozono, K., Nan, H., Hata, T., Saito, K., Kim, YJ., Nagatomo, H., Ohtsuka, T., Koizumi, S., Takiyama, Y.

JOURNAL OF HUMAN GENETICS   67   679 - 686   2022.8( ISSN：1434-5161 )

Novel SLC9A6 Variation in Female Carriers With Intellectual Disability and Atypical Parkinsonism Invited Reviewed

Nan, H., Kim, YJ., Tsuchiya, M., Ishida, A., Haro, H., Hiraide, M., Ohtsuka, T., Takiyama, Y.

Neurology-Genetics   8 ( 1 )   e651   2022.1( ISSN：2376-7839 )

Planar cell polarity protein Vangl2 and its interacting protein Ap2m1 regulate dendritic branching in cortical neurons. Reviewed

Yasumura, M., Hagiwara, A., Hida, Y., Ohtsuka T.

GENES TO CELLS   26 ( 12 )   987 - 998   2021.10( ISSN：1356-9597 )

A Novel Heterozygous Missense Variant in the CIAO1 Gene in a Family with Alzheimer’s Disease: The Val67Ile Variant Promotes the Interaction of CIAO1 and Amyloid-β Protein Precursor. Invited Reviewed

Nan, H., Kim, YJ., Tsuchiya, M., Fukao, T., Hara, N., Hagihara, A., Nishioka, K., Hattori, N., Hara, N., Ikeuchi, T., Ohtsuka, T., Takiyama, Y.*

JOURNAL OF ALZHEIMERS DISEASE   84 ( 2 )   599 - 605   2021.9( ISSN：1387-2877 )

ELKSによる脳－末梢分泌システムの制御機構 Reviewed

濱田駿、大塚稔久

生体の科学　特集「脳とからだ」   72 ( 5 )   2021.9

An engineered channelrhodopsin optimized for axon terminal activation and circuit mapping. Reviewed

Hamada, S., Nagase, M., Yoshizawa, T., Hagiwara, A., Isomura, Y., Watabe, A.M., Ohtsuka, T.*

Communications Biology   4 ( 1 )   461   2021.4

Prickle2 and Igsf9b Coordinately Regulate the Cytoarchitecture of the Axon Initial Segment. Invited Reviewed

Chowdhury, M.I.H., Nishioka, T., Mishima, N., Ohtsuka, T., Kaibuchi, K., Tsuboi, D.

CELL STRUCTURE AND FUNCTION   45 ( 2 )   143 - 154   2020.9( ISSN：0386-7196 )

Neuron-enriched Phosphatase and Actin Regulator 3 (Phactr3)/ Nuclear Scaffold-Associated PP1-inhibiting Protein (Scapinin) Regulates Dendritic Morphology via Its Protein Phosphatase 1-binding Domain. Invited Reviewed

Miyata, T., Kikuchi, K., Ihara, D., Kaito, M., Ishibashi, Y., Hakamata, T., Yamada, T., Ishikawa, M., Mizukoshi, M., Shoji, S., Fukuchi, M., Tsuda, M., Hida, Y., Ohtsuka, T., Kaneda, M., Tabuchi, A.

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   528 ( 2 )   322 - 329   2020.7( ISSN：0006-291X )

RFC1 repeat expansion in Japanese patients with late-onset cerebellar ataxia. Reviewed

Tsuchiya, M., Nan, H., Koh, K., Ichinose, Y., Gao, L., Shimozono, K., Hata, T., Kim, YJ., Ohtsuka, T., Cortese, A., Takiyama, Y.

JOURNAL OF HUMAN GENETICS   65   1143 - 1147   2020.7( ISSN：1434-5161 )

Development of an L-type Ca2+ channel-dependent Ca2+ transient during the radial migration of cortical excitatory neurons. Reviewed

Horigane, S., Hamada, S., Kamijo, S., Yamadaa, H., Yamasaki, M., Watanabe, M., Bito, H., Ohtsuka, T., Takemoto-Kimura, S.

NEUROSCIENCE RESEARCH   169 ( 2021 )   17 - 26   2020.6( ISSN：0168-0102 )

Presynaptic development is controlled by the core active zone proteins CAST/ELKS. Invited Reviewed

Radulovic, T., Dong, W., Goral, R., Thomas, C., Veeraraghavan, P., Montesinos, M., Guerrero-Given, D., Goff, K., Luebbert, M., Kamasawa, N., Ohtsuka, T., Young, S.M.*

JOURNAL OF PHYSIOLOGY   598 ( 12 )   2431 - 2452   2020.6( ISSN：0363-6143 )

Impaired experience-dependent maternal care in presynaptic active zone protein CAST-deficient dams. Reviewed

Hagiwara, A., Sugiyama, N., Ohtsuka, T.

Scientific Reports   10   5238   2020.3( ISSN：2045-2322 )

Double deletion of the active zone proteins CAST/ELKS in the mouse forebrain causes high mortality of newborn pups. Reviewed

Hagiwara, A., Hamada, S., Hida, Y., Ohtsuka, T.

Molecular Brain   13 ( 1 )   13   2020.1( ISSN：1756-6606 )

VPS13D-related disorders presenting as a pure and complicated form of hereditary spastic paraplegia. Reviewed

Kishin Koh, Hiroyuki Ishiura, Haruo Shimazaki, Michiko Tsutsumiuchi, Yuta Ichinose, Haitian Nan, Shun Hamada, Toshihisa Ohtsuka, Shoji Tsuji, Yoshihisa Takiyama

Molecular genetics & genomic medicine   e1108   2019.12

UBAP1 mutations cause juvenile-onset hereditary spastic paraplegias (SPG80) and impair UBAP1 targeting to endosomes. Reviewed

Nan, H., Ichinose, Y., Tanaka, M., Koh, K., Ishiura, H., Mitsui, J., Mizukami, H., Morimoto, M., Hamada, S., Ohtsuka, T., Tsuji, S., Takiyama, Y.

JOURNAL OF HUMAN GENETICS   64 ( 11 )   1055 - 1065   2019.9( ISSN：1434-5161 )

Role of the active zone protein, ELKS, in insulin secretion from pancreatic β-cells. Invited Reviewed Major achievement

Ohara-Imaizumi, M., Aoyagi, K., Ohtsuka, T.

Molecular Metabolism   27S   S81 - S91   2019.9( ISSN：2212-8778 )

Vangl2 interaction plays a role in the proteasomal degradation of Prickle2. Invited Reviewed

Nagaoka, T., Furuse, M., Ohtsuka, T., Tsuchida, K., Kishi, M.

Scientific Reports   9 ( 1 )   2912   2019.2( ISSN：2045-2322 )

ELKS/Voltage-dependent Ca2+ channel-β Subunit Module Regulates Polarized Ca2+ Influx in Pancreatic β-Cells. Invited Reviewed Major achievement

Ohara-Imaizumi, M.*, Aoyagi, K., Hida, Y., Yoshida, M., Yamauchi, H., Ohkura, M., Abe, M., Akimoto, Y., Nakamichi, Y., Nishiwaki, C., Kawakami, H., Hara, K., Sakimura, K., Nakai, J., Kakei, M., Nagamatsu, S., Ohtsuka, T. *

Cell Reports   26   1213 - 1226   2019.1( ISSN：2211-1247 )

CAST/ELKSタンパク質を介したアクティブゾーンの分子構造基盤と生理機能

大塚稔久

生化学   91 ( 6 )   1 - 12   2019

Neuroimaging, genetic, and enzymatic study in a Japanese family with a GBA gross deletion. Invited Reviewed

Ichinose, Y., Ishiura, H., Tanaka, M., Yoshimura, J., Doi, K., Umeda, T., Yamauchi, H., Tsuchiya, M., Koh, K., Yamashiro, N., Mitsui, J., Goto, J., Onishi, H., Ohtsuka, T., Shindo, K., Morishita, S., Tsuji, S., Takiyama, Y.

PARKINSONISM & RELATED DISORDERS   61   57 - 63   2018.12( ISSN：1353-8020 )

神経伝達物質放出マシナリー研究の最前線 Reviewed

大塚稔久

月刊「細胞 The CELL」１２月号「シナプス研究の最先端」   50 ( 14 )   5 - 8   2018.11

Cytomatrix proteins CAST and ELKS regulate retinal photoreceptor development and maintenance. Invited Reviewed Major achievement

Hagiwara, A., Kitahara, Y., Grabner, C.P., Vogl, C., Abe, M., Kitta, R., Ohta, K., Nakamura, K., Sakimura, K., Moser, T., Nishi, A., Ohtsuka, T*.

JOURNAL OF CELL BIOLOGY   218 ( 1 )   3993 - 4006   2018.11( ISSN：0021-9525 )

CAST/ELKS proteins control voltage-gated Ca2+ channel density and synaptic release probability at a mammalian central synapse. Invited Reviewed Major achievement

Dong, W., Radulovic, T., Thomas, C., Montesinos, M.S., Goral, R.O., Given, D.G., Hagiwara, A., Putzke, T., Hida, Y., Abe, M., Sakimura, K., Kamasawa, N., Ohtsuka, T*., Young, S.M*

Cell Reports   24 ( 2 )   284 - 293   2018.7( ISSN：2211-1247 )

Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome. Reviewed

Ichiro Yabe, Hiroaki Yaguchi, Yasutaka Kato, Yasuo Miki, Hidehisa Takahashi, Satoshi Tanikawa, Shinichi Shirai, Ikuko Takahashi, Mari Kimura, Yuka Hama, Masaaki Matsushima, Shinsuke Fujioka, Takahiro Kano, Masashi Watanabe, Shin Nakagawa, Yasuyuki Kunieda, Yoshio Ikeda, Masato Hasegawa, Hiroshi Nishihara, Toshihisa Ohtsuka, Shinya Tanaka, Yoshio Tsuboi, Shigetsugu Hatakeyama, Koichi Wakabayashi, Hidenao Sasaki

Scientific reports   8 ( 1 )   819 - 819   2018.1( ISSN：2045-2322 )

Mutations in bassoon in individuals with familial and sporadic progressive supranuclear palsy-like syndrome. Scientific Reports. Reviewed

Yabe, I., Yaguchi, H., Kato, Y., Miki, Y., Takahashi, H., Tanikawa, S., Shirai, S., Takahashi, I., Kimura, M., Hama, Y., Matsushima, M., Fujioka, S., Kano, T., Watanabe, M., Nakagawa, S., Kunieda, Y., Ikeda, Y., Hasegawa, M., Nishihara, H., Ohtsuka, T., Tanaka, S., Tsuboi, Y., Hatakeyama, S., Wakabayashi, K., Sasaki, H.

Scientific Reports   8 ( 1 )   819 - 819   2018.1( ISSN：2045-2322 )

Synaptic localisation of SRF coactivators, MKL1 and MKL2, and their role in dendritic spine morphology. Reviewed

Kaneda, M., Sakagami, H., Hida, Y., Ohtsuka, T., Satou, N., Ishibashi, Y., Fukuchi, M., Krysiak, A., Ishikawa, M., Ihara, D., Kalita, K., Tabuchi, A.

Scientific Reports   8 ( 1 )   727 - 727   2018.1( ISSN：2045-2322 )

CAST: its molecular structure and phosphorylation-dependent regulation of presynaptic plasticity. Reviewed

Hamada, S., Ohtsuka, T.

NEUROSCIENCE RESEARCH   127   25 - 32   2017.12( ISSN：0168-0102 )

A Wnt5 Activity Asymmetry and Intercellular Signaling via PCP Proteins Polarize Node Cells for Left-Right Symmetry Breaking. Reviewed

Minegishi, K., Hashimoto, M., Ajima, R., Takaoka, K., Shinohara, K., Ikawa, Y., Nishimura, H., McMahon, A., Willert, K., Sasaki, H., Fujimori, T., Ohtsuka, T., Yamaguchi, T., Shimono, A., Shiratori, H., and Hamada, H.

DEVELOPMENTAL CELL   40 ( 5 )   439 - 452   2017.3( ISSN：1534-5807 )

The Auxiliary calcium channel subunit α2δ4 is required for axonal elaboration, synaptic transmission, and wiring of rod photoreceptors. Reviewed

Wang, Y., Fehlhaber, K.E., Sarria, I., Cao, Y., Ingram, N.T., Guerrero-Given, D., Throesch, B., Baldwin, K., Kamasawa, N., Ohtsuka, T., Sampath, A.P., Martemyanov, K.A.

NEURON   93 ( 6 )   1359 - 1374   2017.3( ISSN：0896-6273 )

A de novo mutation in the NALCN gene in an adult patient with cerebellar ataxia associated with intellectual disability and arthrogryposis. Reviewed

CLINICAL GENETICS   90 ( 6 )   556 - 557   2016.10( ISSN：0009-9163 )

SAD-B Phosphorylation of CAST Controls Active Zone Vesicle Recycling for Synaptic Depression. Reviewed Major achievement

Mochida, S.*, ,Hida, Y., ,Tanifuji, S., ,Hagiwara, A., ,Hamada, S., ,Abe, M., ,Huan, M., ,Yasumura, M., ,Kitajima, I., ,Sakimura, K., ,Ohtsuka, T*.

Cell Reports   16 ( 11 )   2901 - 2913   2016.9( ISSN：2211-1247 )

The active zone protein CAST regulates synaptic vesicle recycling and quantal size in the mouse hippocampus. Reviewed Major achievement

Kobayashi, S.,Hida, Y.,Ishizaki, H.,Inoue, E.,Tanaka-Okamoto, M.,Yamasaki, M.,Miyazaki, T.,Fukaya, M.,Kitajima, I.Takai, Y.,Watanabe, M.,Ohtsuka,

EUROPEAN JOURNAL OF NEUROSCIENCE   44 ( 5 )   2272 - 2284   2016.7( ISSN：0953-816X )

Dopamine synapse is a neuroligin-2–mediated contact between dopaminergic presynaptic and GABAergic postsynaptic structures. Reviewed

Uchigashima, M., Ohtsuka, T., Kobayashi, K., Watanabe, M.

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   113 ( 15 )   4206 - 4211   2016.4( ISSN：0027-8424 )

SAD-B kinase regulates presynaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory. Reviewed Major achievement

Watabe,A.M., Nagase, M., Hagiwara, A., Hida, Y., Tsuji, M., ,Ochiai, T., Kato, F., Ohtsuka, T.

JOURNAL OF NEUROCHEMISTRY   136 ( 1 )   36 - 47   2016.1( ISSN：0022-3042 )

SAD-B kinase regulates pre-synaptic vesicular dynamics at hippocampal Schaffer collateral synapses and affects contextual fear memory Reviewed

Ayako M. Watabe, Masashi Nagase, Akari Hagiwara, Yamato Hida, Megumi Tsuji, Toshitaka Ochiai, Fusao Kato, Toshihisa Ohtsuka

JOURNAL OF NEUROCHEMISTRY   136 ( 1 )   36 - 47   2016.1( ISSN：0022-3042  eISSN：1471-4159 )

The planar cell polarity protein Vangl2 bidirectionally regulates dendritic branching in cultured hippocampal neurons. Reviewed Major achievement

Hagiwara A.,Yasumura M.,Hida Y.,Inoue E.,Ohtsuka T.

Molecular Brain   7 ( 1 )   79 - 79   2014.3( ISSN：1756-6606 )

Identification, expression and characterization of rat isoforms of the serum response factor (SRF) coactivator MKL1. Reviewed

Ishikawa Mitsuru,Shiota Jun,Ishibashi Yuta,Hakamata Tomoyuki,Otsuka Toshihisa

FEBS Open Bio   3   387 - 393   2013.9( ISSN：2211-5463 )

前シナプス・アクティブゾーンにおける蛋白質間相互作用 Major achievement

山梨医科学雑誌   27 ( 3 )   109 - 116   2013.5

Vangl2, the planner cell polarity protein, is complexed with postsynaptic density protein PSD-95. Reviewed Major achievement

Yoshioka Toshinori,Hagiwara Akari,Hida Yamato,Otsuka Toshihisa

FEBS LETTERS   587 ( 10 )   1453 - 1459   2013.5( ISSN：0014-5793 )

CAST: functional scaffold for the integrity of the presynaptic active zone. Reviewed Major achievement

Ohtsuka Toshihisa

NEUROSCIENCE RESEARCH   76 ( 1-2 )   10 - 15   2013.3( ISSN：0168-0102 )

The active zone protein CAST regulates synaptic vesicle recycling and quantal size

Kobayashi S, Hida Y, Ishizaki H, Inoue E, Tanaka-Okamoto M, Yamasaki M, Miyazaki T, Fukuya M, Kitajima I, Takai Yoshimi, Watanabe M, Ohtsuka T, Manabe T

J Physiol Sci   63   S67 - S67   2013( ISSN：1880-6546 )

Deletion of the presynaptic scaffold CAST reduces active zone size in rod photoreceptors and impairs visual processing. Reviewed Major achievement

tom Dieck, S., Specht, D., Strenzke, N., Hida, Y., Krishnamoorthy, V., Inoue, E., Ishizaki, H., Tanaka-Okamoto, M., Miyoshi, J., Hagiwara, A., Brandstatter, JH., Gollisch, T., Ohtsuka, T. *, Moser, T*.

JOURNAL OF NEUROSCIENCE   32 ( 35 )   12192 - 12203   2012.8( ISSN：0270-6474 )

Physical and functional interaction of the active zone protein CAST/ERC2 and the β-subunit of the voltage-dependent Ca(2+) channel. Reviewed

Kiyonaka S, Nakajima H, Takada Y, Hida Y, Yoshioka T, Hagiwara A, Kitajima I, Mori Y, Ohtsuka T

Journal of biochemistry   152 ( 2 )   149 - 159   2012.8( ISSN：0021-924X )

Physical and Functional Interaction of the Active Zone Protein CAST/ERC2 and the b-subunit of the Voltage-dependent Ca2+ Channel. Reviewed Major achievement

Kiyonaka Shigeki,Nakajima H,Takada Yoshinori,Hida Yamato,Yoshioka Toshinori,Hagiwara Akari,Kitajima Isao,Mori Yasuo,Otsuka Toshihisa

JOURNAL OF BIOCHEMISTRY   152 ( 2 )   149 - 159   2012.5( ISSN：0021-924X )

前シナプスアクティブゾーンの分子基盤 Major achievement

大塚　稔久

BRAIN & NERVE   63 ( 7 )   641 - 648   2011.7

Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice. Reviewed

Hippocampus   21 ( 6 )   678 - 687   2011.6

Prickle2 is localized in the postsynaptic density and interacts with PSD-95 and NMDA receptors in the brain. Reviewed Major achievement

Hida, Y., Fukaya, M., Hagiwara, A., Deguchi-Tawarada, M., Yoshioka, T., Kitajima, I., Inoue, E., Watanabe, M., Ohtsuka, T. *

JOURNAL OF BIOCHEMISTRY   149 ( 6 )   693 - 700   2011.6( ISSN：0021-924X )

Distribution of serine/threonine kinase SAD-B in mouse peripheral nerve synapse. Reviewed Major achievement

NeuroReport   22 ( 7 )   319 - 325   2011.5

Prickle2 is localized in the postsynaptic density and interacts with PSD-95 and NMDA receptors in the brain. Reviewed Major achievement

J. Biochem.   149 ( 6 )   693 - 700   2011.2

CAST and ELKS Proteins: Structural and Functional Determinants of the Presynaptic Active Zone. Reviewed Major achievement

Hida Yamato,Otsuka Toshihisa

JOURNAL OF BIOCHEMISTRY   148 ( 2 )   131 - 137   2010.8( ISSN：0021-924X )

Reduced expression of the ATRX gene, a chromatin-remodeling factor, causes hippocampal dysfunction in mice. Reviewed

Nogami Tatsuya,Beppu HIdeyuki,Tokoro Takashi,Moriguchi Shigeki,Shioda Norifumi,Hukunaga Kouji,Otsuka Toshihisa,Ishii Yoko,Sasahara Masakiyo,Shimada

HIPPOCAMPUS   21 ( 6 )   678 - 687   2010.3( ISSN：1050-9631 )

生化学者養成ギプス Major achievement

28 ( 9 )   892 - 893   2009.9

Involvement of ELKS, an active zone protein, in exocytotic release from RBL-2H3 cells. Reviewed Major achievement

CELLULAR IMMUNOLOGY   258 ( 2 )   204 - 211   2009.5( ISSN：0008-8749 )

Interaction between Munc13-1 and RIM is Critical for Glucagon-like Peptide-1-mediated Rescue of Exocytotic Defects in Munc13-1-deficient pancreatic β-cells. Reviewed

DIABETES   56 ( 10 )   2579 - 2588   2007.10( ISSN：0012-1797 )

SCRAPPER-dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release (vol 130, pg 943, 2007)

Ikuko Yao, Hiroshi Takagi, Hiroshi Ageta, Tomoaki Kahyo, Showbu Sato, Ken Hatanaka, Yoshiyuki Fukuda, Tomoki Chiba, Nobuhiro Morone, Shigeki Yuasa, Kaoru Inokuchi, Toshihisa Ohtsuka, Grant R. MacGregor, Keiji Tanaka, Mitsutoshi Setou

CELL   131 ( 1 )   190 - 190   2007.10( ISSN：0092-8674 )

SCRAPPER-Dependent ubiquitination of active zone protein RIM1 regulates synaptic vesicle release. Reviewed

CELL   130 ( 5 )   943 - 957   2007.9( ISSN：0092-8674 )

Rab6 regulates transport and targeting of exocytotic carriers. Reviewed

DEVELOPMENTAL CELL   13 ( 2 )   305 - 314   2007.8( ISSN：1534-5807 )

Three-dimensional architecture of presynaptic terminal cytomatrix. Reviewed

JOURNAL OF NEUROSCIENCE   27 ( 26 )   6868 - 6877   2007.6( ISSN：0270-6474 )

The Active Zone Protein CAST Directly Associates with Ligand-of-Numb Protein X. Reviewed Major achievement

Higa Onaga S ,Tokoro T ,Inoue E ,Kitajima I ,Ohtsuka T

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   354 ( 3 )   686 - 692   2007.3( ISSN：0006-291X )

Localization of the Active Zone Proteins CAST, ELKS, and Piccolo at Neuromuscular Junctions. Reviewed Major achievement

NEUROREPORT   18 ( 4 )   313 - 316   2007.3( ISSN：0959-4965 )

SAD kinase directly phosphorylates the active zone proteins Bassoon and Piccolo Reviewed

Higa Susumu, Tokoro Takashi, Kitajima Isao, Ohtsuka Toshihisa

NEUROSCIENCE RESEARCH   58   S74   2007( ISSN：0168-0102 )

SAD kinase is a presynaptic component of mouse neuromuscular junctions Reviewed

Tokoro Takashi, Yoshida Shoko, Higa Susumu, Kitajima Isao, Ohtsuka Toshihisa

NEUROSCIENCE RESEARCH   58   S132   2007( ISSN：0168-0102 )

CLASPs attach microtubule plus ends to the cell cortex through a complex with LL5β. Reviewed

Lansbergen, G., Grigoriev, I., Mimori-Kiyosue, Y., Ohtsuka, T., Higa, S., Kitajima, I., Demmers, J., Galjart, N., Houtsmuller, A.B., Grosveld, F., Akhmanova, A.

Developmental Cell   11 ( 1 )   21 - 32   2006.7

ELKS, a protein structurally related to the active zone protein CAST, regulates Ca2+-dependent exocytosis from PC12 cells. Reviewed Major achievement

Inoue, E., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Kitajima, I., Ohtsuka, T.*, Takai, Y.

Genes Cells   11 ( 6 )   659 - 672   2006.6

SAD: a presynaptic kinase associated with synaptic vesicles and the active zone cytomatrix that regulates neurotransmitter release. Reviewed Major achievement

Inoue, E., Mochida, S., Takagi, H., Higa, S., Deguchi-Tawarada, M., Takao-Rikitsu, E., Inoue, M., Yao, I., Takeuchi, K., Kitajima, I., Setou, M., Ohtsuka, T.*, Takai, Y.

Neuron   50 ( 2 )   261 - 275   2006.4

Active zone protein CAST is a component of conventional and ribbon synapses in mouse retina. Reviewed Major achievement

Deguchi-Tawarada, M., Inoue, E., Takao-Rikitsu, E., Inoue, M., Ktajima, I., Ohtsuka, T. *, Takai, Y.

J. Comp. Neurol.   495 ( 4 )   480 - 496   2006.4

Differential distribution of release-related proteins in the hippocampal CA3 area as revealed by freeze-fracture replica labeling. Reviewed

Hagiwara, A., Fukazawa, Y., Deguchi-Tawarada, M., Ohtsuka, T., Shigemoto, R.

J. Comp. Neurol.   489 ( 2 )   195 - 216   2005.8

ELKS, a protein structurally related to the active zone-associated protein CAST, is expressed in pancreatic β cells and functions in insulin exocytosis・・・ Reviewed Major achievement

Ohara-Imaizumi, M., Ohtsuka, T., Matsushima, S., Akimoto, Y., Nishiwaki, C., Nakamichi, Y., Kikuta, T., Nagai, S., Kawakami, H., Watanabe, T., and Nagamatsu, S.

Mol. Biol. Cell.   16 ( 7 )   3289 - 3300   2005.7

Roles of cell-adhesion molecules nectin 1 and nectin 3 in ciliary body development. Reviewed

Inagaki, M., Irie, K., Ishizaki, H., Tanaka-Okamoto, M., Morimoto, K., Inoue, E., Ohtsuka, T., Miyoshi, J., Takai, Y.

Development   132 ( 7 )   1525 - 1537   2005.4

Nectin-like molecule-1/TSLL1/SynCAM3: a neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional contact sites of presynaptic nerve terminals, axons and glia cell processes. Reviewed

Kakunaga, S., Ikeda, W., Itoh, S., Deguchi-Tawarada, M., Ohtsuka, T., Mizoguchi, A., Takai, Y.

J. Cell Sci.   118 ( Pt6 )   1267 - 1277   2005.3

Nectin-like molecule-1/TSLL1/SynCAM-3: A neural tissue-specific immunoglobulin-like cell-cell adhesion molecule localizing at non-junctional adhesion sites where presynaptic nerve terminals, axons, and glia cell processes contact Reviewed

Shipeki Kakunaga, Wataru Ikeda, Shinsuke Itoh, Maki Deguchi-Tawarada, Toshihisa Ohtsuka, Akira Mizoguchi, Yoshimi Takai

CELL STRUCTURE AND FUNCTION   29   42 - 42   2004.5( ISSN：0386-7196  eISSN：1347-3700 )

Physical and functional interaction of the active zone proteins, CAST, RIM1, and Bassoon in neurotransmitter release. Reviewed Major achievement

Takao-Rikitsu, E., Mochida, S., Inoue, E., Deguchi-Tawarada, M., Inoue, M., Ohtsuka, T.*, Takai, Y.

J. Cell Biol.   164 ( 2 )   301 - 311   2004.1

CAST2: identification and characterization of a protein structurally related to the presynaptic cytomatrix protein CAST. Reviewed

Deguchi-Tawarada, M., Inoue, E., Takao-Rikitsua, E., Inoue, M., Ohtsuka, T.*, Takai, Y.

Genes Cells   9 ( 1 )   15 - 23   2004.1

The roles of cadherins and nectins in interneuronal synapse formation. Reviewed

Takai, Y., Shimizu, K., and Ohtsuka, T.

Curr. Opin. Neurobiol. Review.   13 ( 5 )   520 - 526   2003.12

Nectin-dependent localization of synaptic scaffolding molecule (S-SCAM) at the puncta adherentia junctions formed between the mossy fibre terminals and the dendrites of pyramidal cells in the CA3 area of the mouse hippocampus. Reviewed

Yamada, A., Irie, K., Deguchi-Tawarada, M., Ohtsuka, T., Takai, Y.

Genes Cells   8 ( 12 )   985 - 994   2003.12

Nectin-dependent localization of ZO-1 at puncta adhaerentia junctions between the mossy fiber terminals and the dendrites of the pyramidal cells in the CA3 area of adult mouse hippocampus. Reviewed

Inagaki, M., Irie, K., Deguchi-Tawarada, M., Ikeda, W., Ohtsuka, T., Takeuchi, M., Takai, Y.

J. Comp. Neurol. 460(4):514-524,   460 ( 4 )   514 - 524   2003.6

CAST: a novel protein of the cytomatrix at the active zone of synapses that forms a ternary complex with RIM1 and Munc13-1. Reviewed Major achievement

Ohtsuka, T.*, Takao-Rikitsu, E., Inoue, E., Inoue, M., Takeuchi, M., Matsubara, K., Deguchi- Tawarada, M., Satoh, K., Morimoto, K., Nakanishi, H., Takai, Y.

J. Cell Biol.   158 ( 3 )   577 - 590   2002.8

Membrane-associated guanylate kinase with inverted orientation (MAGI)-1/brain angiogenesis inhibitor 1-associated protein (BAP1) as a scaffolding molecule for Rap small G protein GDP/GTP exchange protein at tight junctions. Invited Reviewed

Mino, A, Ohtsuka, T., Inoue, E., Takai, Y.

Genes Cells   5 ( 12 )   1009 - 1016   2000.12

Importance of spatial activation of Cdc42 and rac small G proteins by frabin for microspike formation in MDCK cells. Invited Reviewed

Yasuda, T, Ohtsuka, T., Inoue, E., Yokoyama, S., Sakisaka, T., Kodama, A., Takahashi, K., Taka, Y.

Genes Cells   5 ( 7 )   583 - 591   2000.7

Association of membrane- associated guanylate kinase-interacting protein-1 with Raf-1. Invited Reviewed

Yao, I., Ohtsuka, T., Kawabe, H., Matsuura, Y., Takai, Y., Hata, Y.

Biochem. Biophys. Res. Commun.   270 ( 2 )   538 - 542   2000.4

nRap GEP: a novel neural GDP/GTP exchange protein for rap1 small G protein that interacts with synaptic scaffolding molecule (S-SCAM). Invited Reviewed Major achievement

Ohtsuka, T., Hata, Y., Ide, N., Yasuda, T., Inoue, E., Inoue, T., Mizoguchi, A., and Takai, Y.

Biochem. Biophys. Res. Commun.   265 ( 1 )   38 - 44   1999.11

Rab11BP/Rabphilin-11, a downstream target of rab11 small G protein implicated in vesicle recycling. Invited Reviewed

Mammoto, A., Ohtsuka, T., Hotta, I., Sasaki, T., and Takai, Y.

J. Biol. Chem.   274 ( 36 )   25517 - 25524   1999.9

Nexilin: a novel actin filament-binding protein localized at cell-matrix adherens junction. Invited Reviewed Major achievement

Ohtsuka, T., Nakanishi, H., Ikeda, W., Satoh, A., Momose, Y., Nishioka, H., and Takai, Y.

J. Cell Biol.   143 ( 5 )   1227 - 1238   1998.11

Cell-free assay system for Ras- and Rap1-dependent activation of MAP-kinase cascade. Invited Reviewed

Shimizu, K., Ohtsuka, T., Takai, Y.

Methods Mol. Biol. Review.   84   173 - 183   1998

Different effects of various phospholipids on Ki-Ras-, Ha-Ras-, and Rap1B-induced B-Raf activation. Invited Reviewed

Kuroda, S., Ohtsuka, T., Yamamori, B., Fukui, K., Shimizu, K., and Takai, Y.

J. Biol. Chem.   271 ( 25 )   14680 - 14683   1996.6

Activation of brain B-Raf protein kinase by Rap1B small GTP-binding protein. Reviewed Major achievement

JOURNAL OF BIOLOGICAL CHEMISTRY   271 ( 3 )   1258 - 1261   1996.1( ISSN：0021-9258 )

Purification of a Ras-dependent mitogen-activated protein kinase kinase kinase from bovine brain cytosol and its identification as a complex of B-Raf and 14-3-3 proteins. Invited Reviewed

Yamamori, B., Kuroda, S., Shimizu, K., Fukui, K., Ohtsuka, T., Takai, Y.

J. Biol. Chem.   270 ( 20 )   11723 - 11726   1995.5

PURIFICATION OF A RAS-DEPENDENT MITOGEN-ACTIVATED PROTEIN-KINASE KINASE KINASE FROM BOVINE BRAIN CYTOSOL AND ITS IDENTIFICATION AS A COMPLEX OF B-RAF AND 14-13-3-PROTEINS Invited Reviewed

B YAMAMORI, S KURODA, K SHIMIZU, K FUKUI, T OHTSUKA, Y TAKAI

JOURNAL OF BIOLOGICAL CHEMISTRY   270 ( 20 )   11723 - 11726   1995.5( ISSN：0021-9258 )

Doc2: a novel brain protein having two repeated C2-like domains. Invited Reviewed

Orita, S., Sasaki, T., Naito, A., Komuro, R., Ohtsuka, T., Maeda, M., Suzuki, H., Igarashi, H., Takai, Y.

Biochem. Biophys. Res. Commun.   206 ( 2 )   439 - 448   1995.1

Depletion of LKB1 in the cerebellar granule cells induces locomotor dysfunction in aged mice. International conference

Mayu Kitamura, Akari Hagiwara, Shun Hamada, and Toshihisa Ohtsuka

NEURO2022  2022.7  The Japan Neuroscience Society

Morphological examination of the role of LKB1 in retinal horizontal cell remodeling using the AAV-based labeling system. 　 International conference

Saki Kawamura, Toshihisa Ohtsuka, Yamato Hida, Akari Hagiwara

NEURO2022  2022.7  The Japan Neuroscience Society

記憶の仕組み～覚えたい？忘れたい？ Invited

大塚稔久

読売新聞社・山梨大学共同主催　連続市民講座  2021.9  読売新聞社・山梨大学共同主催

Role of CAST/ELKS in the active zone function via liquid-liquid phase separation International conference

Ohtsuka Toshihisa

The 44th Annual Meeting of the Japan Neuroscience Society  2021.7 

The active zone protein CAST/ELKS controls the liquid-liquid phase separation of RIM1 Invited

Toshihisa Ohtsuka

第6回「脳情報動態」領域会議  2021.3  「脳情報動態」事務局

生体膜ダイナミクス:細胞レベルの制御機構から個体レベルの生理機能まで Invited

大塚稔久

第93回日本生化学会大会公募シンポジウム  2020.9  日本生化学会事務局

CAST Interactome: molecular diversity of release probability in multiple brain areas Invited

Toshihisa Ohtsuka

2019.12 

Symposium “Neuropsychopharmacology of relaxin-3” International conference

Toshihisa Ohtsuka

AsCNP2019  2019.10 

シナプス分子間相互作用から捉える可塑性機構とその破綻 Invited

大塚稔久

日本蛋白質科学会年会・日本細胞生物学会合同年次大会  2019.6  日本細胞生物学会

Biochemical analysis of the presynaptic active zone protein CAST in the maternal-like behavior-related neuronal circuits

適応回路シフト領域班会議（冬の班会議）および成果報告ンポジウム  2018.12 

Functional roles of CAST/ELKS proteins in retinal photoreceptor development and maintenance.

Toshihisa Ohtsuka

スクラップビルド第3回領域会議  2018.11 

神経シナプス・アクティブゾーン：分子構造から脳病態の理解へ Invited

大塚稔久

第34回和光ワークショップ　神経科学：基礎から臨床  2018.11  富士フィルム和光純薬株式会社

Molecular structure and function of the presynaptic active zone. Invited

Toshihisa Ohtsuka

A symposium in honor of Sumiko Mochida-Forefront of Neurotransmitter Release and Calcium Channel Signaling-  2018.7 

Possible roles of the active zone proteins in neural circuits underlying reward activity in mice Invited

Toshihisa Ohtsuka

第95回日本生理学会大会　  2018.3 

Molecular Mechanisms of Presynaptic Transmission. Invited International conference Major achievement

Toshihisa Ohtsuka

The first international symposium for frontend brain science: University of Yamanashi 2018.  2018.2 

Integral system by CAST/ELKS protein family to regulate retinal photoreceptor development maintenance Invited International conference

Toshihisa Ohtsuka

BRI International Symposium 2018  2018.2 

神経終末アクティブゾーンの生理機能とその破綻　～分子構造から高次脳機能の解明へ～ Invited

大塚稔久

学生講義＆セミナー  2017.7 

CAST phosphorylation in control of synaptic plasticity at the excitatory synapses” Invited International conference Major achievement

Toshihisa Ohtsuka

The 47th NIPS International Symposium "Decoding Synapses"  2016.10 

神経伝達物質と脳・シナプス病態の解析

大塚稔久

山梨大学医学部セミナー  2016.10  山梨大学医学部

「神経伝達物質放出の分子構造基盤とその破綻による精神神経疾患」 Major achievement

大塚稔久

大阪大学医学部セミナー  2016.5  大阪大学医学部

神経終末アクティブゾーンの分子構造基盤とその破綻～構造分子解析から脳高次機能の解明へ向けて～ Major achievement

大塚稔久

群馬大学医学部大学院セミナー  2016.4  群馬大学医学部

プレシナプス・キナーゼSAD-Bによるシナプス小胞ダイナミクスと可塑性制御の分子基盤 Invited Major achievement

大塚稔久

生理学研究所研究会「シナプス・神経ネットワークの機能ダイナミクス」  2015.12  生理学研究所研究会

The active zone proteins CAST and ELKS regulate the structure and function of retinal ribbon synapses Invited International conference Major achievement

Ribbon Synapses Symposium.  2015.9 

神経終末アクティブゾーンの分子構造基盤とその破綻　～構造分子解析から脳高次機能の解明へ向けて～ Major achievement

大塚稔久

東京医科歯科大学セミナー  2015.6  東京医科歯科大学

CAST and ELKS: molecular determinants for the presynaptic active zone Invited International conference Major achievement

マックスプランクフロリダ研究所セミナー マイアミUSA(Host: Dr. Sam young)  2015.6 

神経伝達物質の放出から捉える脳の高次機能とその破綻 Major achievement

第10回山梨脳・血管セミナー  2015.4  第10回山梨脳・血管セミナー

神経終末アクティブゾーンの分子構造基盤とその破綻～プレシナプスから精神神経疾患の発症機構を捉える～ Major achievement

大塚稔久

東京都医学総合研究所招待講演  2014.8  東京都医学総合研究所

神経終末アクティブゾーンの分子構造基盤と生理機能～分子から精神神経疾患の病態解明へ向けて～ Major achievement

大塚稔久

北里大学医学会招待学術講演会  2014.7  北里大学医学会

CAST regulates the structure and function of presynaptic active zones Major achievement

第36回日本分子生物学会　講演発表  2013.12 

The presynaptic active zone molecular organization and physiological functions. Major achievement

神経構造生物学合同研究会　  2013.11 

CAST and vesicle cycling at the ribbon synapses Major achievement

Ribbon Synapses Symposium.  2013.9 

CAST Phosphorylation Mediates Short-term Synaptic Depression. Major achievement

Neuro2013  2013.6 

翻訳後修飾によるプレシナプス・アクティブゾーンの機能制御 Major achievement

生理学研究所シナプス研究会「神経シナプス伝達の時空間ダイナミクス」  2012.11 

Structure and function of the presynaptic active zone Major achievement

Otsuka Toshihisa

International Synapse Research Workshop 2012  2012.11 

Role of CAST in the structure and function of ribbon synapses International conference Major achievement

Otsuka Toshihisa

Ribbon Synapses Symposium.  2011.9 

神経伝達物質の放出の動作原理とその破綻 Major achievement

国立精神・神経医療研究センター合同シンポジウム  2010.11 

神経伝達物質放出の分子構造基盤と回路制御 Major achievement

第24回山梨神経科学研究会  2010.11 

「トランスミッター放出マシナリータンパク複合体の分子構造基盤」 Major achievement

大塚　稔久

第52回日本神経化学会大会シンポジウム  2009.6 

The Active Zone Protein CAST is a Component of Conventional and Ribbon Synapses in Mouse Retina. International conference Major achievement

Otsuka Toshihisa

Ribbon Synapses Symposium: Molecular anatomy and physiology of ribbon synapses.  2009.5 

「神経終末アクティブゾーンの分子構造基盤と生理機能」 Major achievement

大塚　稔久

生化学学会九州支部シンポジウム  2009.5 

教養総合講義

2023Year  Type of subject：Common education (undergraduate)

学問としての医学-実験医学序説

高校生への公開授業　 記憶って何？

Role(s)： Lecturer

山梨大学  高校生への公開授業　  山梨大学医学部  2021.7

若桐講座

Role(s)： Lecturer

山梨大学教育学部附属教育実践総合センター  附属中学校の生徒、及び、保護者を対象に９０分講義 生徒50名、保護者27名の計77名 記憶のしくみ～覚えたい？忘れたい？  山梨大学甲府キャンパス  2019.9

山梨大学医学部オープンキャンパス　 基礎医学の魅力・脳は「脳 」を理解できるか！？

Role(s)： Lecturer

山梨大学  山梨大学医学部  2019.8

高校生への公開授業　 記憶って何？

Role(s)： Lecturer

山梨大学  2019.7

若桐講座

Role(s)： Lecturer

山梨大学教育学部附属教育実践総合センター  附属中学校の生徒、及び、保護者を対象に９０分講義 生徒50名、保護者27名の計77名 記憶のしくみ～覚えたい？忘れたい？  山梨大学甲府キャンパス  2018.9

山梨大学医学部オープンキャンパス　 基礎医学の魅力・脳は「脳 」を理解できるか！？

Role(s)： Lecturer

2018.8

高校生への公開授業　 記憶って何？

Role(s)： Lecturer

山梨大学  2018.7

「生化学」誌企画委員

日本生化学会  2013.1 - 2018.12

「生化学」誌企画委員

日本生化学会  2013.1 - 2018.12

日本神経科学学会

2004.8

日本細胞生物学学会

2004.5

日本分子生物学学会

2002.8

日本神経科学学会   第４6回日本神経科学大会実行委員長  

2022.4 - 2023.8   

日本生化学会関東支部例会   日本生化学会関東支部例会幹事世話人代表  

2022.4 - 2023.7   

日本生化学会医学会 医学用語委員会   日本生化学会医学会 医学用語委員  

2022.4 - 2023.3   

生理研研究会　   生理研研究会代表  

2022.4 - 2023.3   

国立研究開発法人日本医療研究開発機構   AMED課題評価委員会（認知症研究開発事業）  

2021.12 - 2023.3   

国立研究開発法人日本医療研究開発機構   AMED課題評価委員会（認知症研究開発事業）  

2021.12 - 2023.3   

日本神経科学学会   第４５回日本神経科学大会実行委員  

2020.7 - 2022.11   

日本神経科学会   副庶務理事  

2020.1 - 2022.12   

日本神経科学学会   第４４回日本神経科学大会実行委員  

2019.12 - 2021.11   

日本神経科学学会   第４３回プログラム委員  

2019.4 - 2020.7   

日本神経科学学会   第４２回日本神経科学会プログラム委員  

2019.4 - 2019.7   

山梨県医師会   生涯教育委員会委員  

2018.8   

国立研究開発法人日本医療研究開発機構   戦略的国際脳科学研究推進プログラム　プログラムオフィサー  

2018.4   

国立研究開発法人日本医療研究開発機構   戦略的国際脳科学研究推進プログラム課題評価委員会委員  

2018.4   

国立研究開発法人日本医療研究開発機構   難治性疾患実用化研究事業課題評価委員会専門委員  

2017.12   

国立研究開発法人日本医療研究開発機構   AMED新規事業CiCLEの専門委員（査読）  

2017.8   

国立研究開発法人日本医療研究開発機構   革新的技術による脳機能ネットワークの全容解明プロジェクトプログラムオフィサー  

2017.4   

国立研究開発法人日本医療研究開発機構   AMED課題評価委員  

2017.4   

国立研究開発法人日本医療研究開発機構   AMED課題評価委員  

2017.4   

日本神経科学会   庶務理事  

2016.10 - 2019.12   

一般社団法人　日本神経精神薬理学会   総務委員会　委員  

2016.10 - 2018.10   

第41回日本神経科学大会実行委員　トラベルアワード選考委員長   実行委員トラベルアワード選考委員長  

2016.7 - 2018.7