カルシウムイオン依存的な鞭毛構築機構の解明

2019.4 - 2021.3

若手研究

久保智広

チューブリンポリグルタミン酸化修飾による鞭毛構築機構の解明

2017.11

公益財団法人　武田科学振興財団 

久保智広

チューブリンポリグルタミン酸化修飾による鞭毛繊毛構築メカニズムの解明

2017.4 - 2019.3

若手研究B

久保智広

チューブリンポリグルタミン酸化修飾による鞭毛・繊毛運動機構の追究

2016.10 - 2017.10

公益財団法人金原一郎記念医学医療振興財団  基礎医学医療研究助成金報告書  基礎医学医療研究

久保智広

CFAP70 is a Novel Axoneme-Binding Protein that Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Reviewed Major achievement

Shamoto N, Narita K, Kubo T, Oda T, and Takeda S.

Cells   2018.8( ISSN：2073-4409 )

A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1). Reviewed Major achievement

MOLECULAR BIOLOGY OF THE CELL   2018.3( ISSN：1059-1524 )

Electrostatic interaction between polyglutamylated tubulin and the nexin-dynein regulatory complex regulates flagellar motility. Reviewed Major achievement

Tomohiro Kubo and Toshiyuki Oda

MOLECULAR BIOLOGY OF THE CELL   2017.6( ISSN：1059-1524 )

RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Reviewed Major achievement

Yuya Nishijima, Yohei Hagiya, Tomohiro Kubo, Ryota Takei, Yohei Katoh, and Kazuhisa Nakayama

MOLECULAR BIOLOGY OF THE CELL   28 ( 12 )   1652 - 1666   2017.6( ISSN：1059-1524 )

Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. Reviewed Major achievement

JOURNAL OF CELL SCIENCE   129 ( 10 )   2106 - 2119   2016.5( ISSN：0021-9533 )

Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Reviewed Major achievement

MOLECULAR BIOLOGY OF THE CELL   26 ( 15 )   2810 - 2822   2015.8( ISSN：1059-1524 )

Assembly of IFT Trains at the Ciliary Base Depends on IFT74. Reviewed

CURRENT BIOLOGY   25   1583 - 1593   2015.6( ISSN：0960-9822 )

A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9. Reviewed Major achievement

MOLECULAR BIOLOGY OF THE CELL   25   107 - 117   2014.1( ISSN：1059-1524 )

Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Reviewed Major achievement

Cytoskeleton   69 ( 12 )   1059 - 1068   2012.12( ISSN：1949-3584 )

Tubulin polyglutamylation regulates axonemal motility by modulating activities on inner-arm dyneins. Reviewed Major achievement

Current Biology   20 ( 5 )   441 - 445   2010.3( ISSN：0960-9822 )

Ca2+ signaling surpasses the suppression of flagellar assembly in the axonemal dynein-lacking mutant Major achievement

Natsumi Kanou, Tomohiro Kubo1, and Toshiyuki Oda1

2020.9 

Identification of polyglutamylation sites in Chlamydomonas alpha-tubulin Major achievement

Tomohiro Kubo and Toshiyuki Oda1

2020.9 

カルシウムイオン依存的な鞭毛構築機構の解明

久保智広、加納夏実、小田賢幸

繊毛研究会  2019.11 

A Microtubule-Dynein Tethering Complex Regulates the Axonemal Inner Dynein f (I1) International conference Major achievement

Tomohiro Kubo

18th International Conference on the Cell and Molecular Biology of Chlamydomonas  2018.6 

A microtubule-dynein tethering complex regulates the axonemal inner dynein (I1)

Tomohiro Kubo, Yuqing Hou, Deborah Cochran, George Witman and Toshiyuki Oda

70th Annual Meeting of Japan Society for Cell Biology  2018.6 

The IFT81 and IFT74 N-termini together form the main module for intraflagellar transport(IFT) of tubulin Invited Major achievement

Tomohiro Kubo、Jason Brown, Karl Bellve, Branch Craige, Julie Craft, Kevin Forgarty, Karl Lechtreck, George Witman

2017.6 

Structural biology of Chlamydomonas flagella Invited Major achievement

Tomohiro Kubo and Toshiyuki Oda

2017.5 

The IFT81 and IFT74 N-termini together form the main module for intraflagellar transport(IFT) of tubulin Major achievement

Tomohiro Kubo, Jason Brown, Karl Bellve, Branch Craige, Julie Craft, Kevin Forgarty, Karl Lechtreck, George Witman

2016.11 

Axonemal tubulin polyglutamylation deficiency suppresses flagellar shortness Major achievement

2015.9 

The IFT81 tubulin-binding domain and the IFT74 N-terminus individually are dispensable but　together are crucial for flagellar assembly International conference Major achievement

Tomohiro Kubo, Jason M. Brown, Branch Craige, and George B. Witman

FASEB (Biology of Cilia and Flagella)  2015.6 

A novel Chlamydomonas IFT81 mutant reveals that the IFT81 tubulin-binding domain is not crucial for tubulin transport and flagellar assembly International conference Major achievement

Tomohiro Kubo, Jason M. Brown, George B. Witman

The American Society for Cell Biology 2014  2014.12 

IFT74 is required for IFT-A/IFT-B interaction, but not for import of tubulin into flagella. International conference Major achievement

Jason M. Brown, Deborah A. Cochran, Tomohiro Kubo, and George B. Witman

16th International Conference on the Cell and Molecular Biology of　Chlamydomonas  2014.6 

Axonemal tubulin polyglutamylation modulates flagellar length by enhancing tubulin turnover at the flagellar tip International conference Major achievement

Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, Ritsu Kamiya, and George B. Witman

16th International Conference on the Cell and Molecular Biology of　Chlamydomonas  2014.6 

The N terminus of the IFT complex B protein IFT74 is required for normal flagellar import of complex A and BBS4 tracking, but not for tubulin import or flagellar assembly International conference Major achievement

Jason M. Brown, Deborah A. Cochran, Tomohiro Kubo, and George B. Witman.

The American Society for Cell Biology 2013  2013.12  The American Society for Cell Biology

A suppressor mutant, ssh1, of flagellar shortness in dynein-deficient Chlamydomonas strains is　 deficient in the gene encoding FAP234, a protein involved in tubulin polyglutamylation International conference Major achievement

Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, George B. Witman, and Ritsu Kamiya

The American Society for Cell Biology 2013  2013.12  The American Society for Cell Biology

Regulation of Axonemal Dynein Activity by a Conserved Tubulin Polyglutamylation System International conference Major achievement

Tomohiro Kubo, Zhongmei Liu, Takumi Aikawa, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya

Dynein 2013 International Workshop  2013.11 

Regulation of Axonemal Dynein Activity by a Conserved Tubulin Polyglutamylation System Major achievement

Tomohiro Kubo, Zhongmei Liu, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya

The 25th CDB Meeting; Cilia and Centrosomes: from Fertilization to Cancer  2013.6 

鞭毛運動のチューブリン・ポリグルタミン酸化修飾による調節 Major achievement

久保智広、八木俊樹、神谷律

生体運動班会議  2013.1 

A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein crucial for the localization of TTLL9, an enzyme that catalyzes tubulin polyglutamylation in the axoneme International conference Major achievement

omohiro Kubo, Yanagisawa Haru-aki, Zhongmei Liu, Rie Shibuya, Masafumi Hirono, and Ritsu Kamiya

The American Society for Cell Biology 2012  2012.12  The American Society for Cell Biology

Tubulin polyglutamylation regulates axonemal motility by modulating the function of a particular inner-arm dynein species (dynein e) Major achievement

Tomohiro Kubo, Toshiki Yagi, and Ritsu Kamiya

2012.9 

Tubulin polyglutamylation regulates axonemal motility by modulating the function of a particular inner-arm dynein species (dynein e). International conference Major achievement

Tomohiro Kubo, Toshiki Yagi, and Ritsu Kamiya

15th International Conference on the Cell and Molecular Biology of Chlamydomonas  2012.6 

Tubulin polyglutamylation regulates flagellar motility by modulating the function of inner arm dynein “e” International conference Major achievement

Tomohiro Kubo, Toshiki Yagi, and Ritsu Kamiya

The American Society for Cell Biology 2011  2011.12  The American Society for Cell Biology

軸糸チューブリン・ポリグルタミン酸化修飾に関与する新規鞭毛蛋白質の同定 Major achievement

久保智広、柳澤春明、劉中美、廣野雅文、神谷律

第81回日本動物学会  2011.9  日本動物学会

Tubulin Polyglutamylation Regulates Flagellar Motility Through the Function of Dynein Regulatory Complex Major achievement

Tomohiro Kubo and Ritsu Kamiya

2011.9 

A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein associated with TTLL9, an enzyme that catalyzes tubulin polyglutamylation in the axoneme Major achievement

Tomohiro Kubo, Haru-aki Yanagisawa, Zhongmei Liu, Masafumi Hirono, and Ritsu Kamiya

2011.6 

チューブリン・ポリグルタミン酸化修飾による軸糸ダイニンと鞭毛運動の調節 Major achievement

久保智広、柳澤春明、八木俊樹、廣野雅文、神谷律

生体運動合同班会議  2010.11 

Tubulin Polyglutamylation Regulates Flagellar Motility by Affecting Functions of Inner-Arm Dyneins Major achievement

2010.9 

Tubulin Polyglutamylation Regulates Flagellar Motility by Affecting Functions of Inner-Arm Dyneins Major achievement

Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, Ritsu Kamiya

第62回日本細胞生物学会  2010.5  日本細胞生物学会

Importance of Tubulin Polyglutamylation for Axonemal Dynein Function as Revealed by a Novel Chlamydomonas Mutant International conference Major achievement

Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, Ritsu Kamiya

2009.11 

クラミドモナス鞭毛運動におけるチューブリン・ポリグルタミル化修飾の重要性 Major achievement

久保智広、柳澤春明、八木俊樹、廣野雅文、神谷律

第61回日本細胞生物学会  2009.6  日本細胞生物学会

Importance of tubulin polyglutamylation for cilia/flagella motility as revealed by a novel a Chlamydomonas mutant International conference Major achievement

Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya

Gordon Research Conference 2009 (Cilia, Mucus & Mucociliary Interactions)  2009.2 

クラミドモナス変異株で明らかになった鞭毛チューブリン翻訳後修飾の機能的重要性 Major achievement

久保智広、柳澤春明、八木俊樹、神谷律

日本動物学会第79回大会  2008.9  日本動物学会

解剖学A

2018Year  Type of subject：Common education (undergraduate)

解剖学B

2018Year

解剖学A Major achievement

2017Year  Type of subject：Common education (undergraduate)

分子組織学 Major achievement

2017Year  Type of subject：Common education (undergraduate)