Organization
Graduate Faculty of Interdisciplinary Research Faculty of Medicine Basic Science for Clinical Medicine (Anatomy and Structural Biology) Senior Assistant Professor
Title
Senior Assistant Professor
Contact information
Kubo Tomohiro
Research History
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University of Yamanashi Senior Assistant Professor
2019.4
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山梨大学 特任助教 助教
2016.4 - 2019.3
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Postdoctoral Research Associate (University of Massachusetts Medical School)
2013.4 - 2016.3
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日本学術振興会特別研究員（PD）
2012.4 - 2013.3
Notes：2012/04/01-2013/03/31
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日本学術振興会特別研究員(DC2)
2011.4 - 2012.3
Notes：2011/04/01-2012-3/31
Education
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The University of Tokyo
- 2012.3
Country： Japan
Course： Doctor course
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The University of Tokyo
- 2009.3
Country： Japan
Course： Master course
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The University of Tokyo
- 2007.3
Country： Japan
Degree
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Ph.D ( 2012.3 The University of Tokyo )
Current state of research and teaching activities
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クラミドモナスという単細胞緑藻類を用いて、真核生物の鞭毛（繊毛と同義）の構築機構と運動機構を研究している。
Research Areas
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Life Science / Cell biology
Research Interests
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Cilia/flagella, Axonemal dynein, Chlamydomonas reinhardtii, Posttranslational modification, Tubulin polyglutamylation, Intraflagellar transport
Subject of research
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Studies on the regulation of flagellar/ciliary motility by tubulin polyglutamylation
Research Projects
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真核生物の鞭毛で新規に見つかった蛋白質合成系の解明
2023.4 - 2026.3
基盤研究C
久保智広
Authorship：Principal investigator Type of fund：：Science research expense
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真核生物の鞭毛に局在する蛋白質合成系の機能解明
2023.4 - 2025.3
久保智広
Authorship：Principal investigator Type of fund：：Science research expense
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微細藻類の鞭毛に存在する蛋白質合成系の解明
2023.4 - 2025.3
久保智広
Authorship：Principal investigator Type of fund：：Science research expense
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鞭毛繊毛の構築に必須なチューブリン供給システムの解明
2022.4 - 2023.3
公益財団法人 小柳財団 研究助成金
久保智広
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鞭毛構築に必須な鞭毛前駆体の実体解明
2021.4 - 2022.3
上原記念生命科学財団 研究奨励金
久保智広
Authorship：Principal investigator Type of fund：：Science research expense
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カルシウムイオン依存的な鞭毛構築機構の解明
2019.4 - 2021.3
若手研究
久保智広
Authorship：Principal investigator Type of fund：：Science research expense
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チューブリンポリグルタミン酸化修飾による鞭毛構築機構の解明
2017.11
公益財団法人 武田科学振興財団
久保智広
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チューブリンポリグルタミン酸化修飾による鞭毛繊毛構築メカニズムの解明
2017.4 - 2019.3
若手研究B
久保智広
Authorship：Principal investigator Type of fund：：Science research expense
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チューブリンポリグルタミン酸化修飾による鞭毛・繊毛運動機構の追究
2016.10 - 2017.10
公益財団法人金原一郎記念医学医療振興財団 基礎医学医療研究助成金報告書 基礎医学医療研究
久保智広
Authorship：Principal investigator Type of fund：：Others
Papers
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Tubulin glycylation controls ciliary motility through modulation of outer-arm dyneins Reviewed Major achievement
Tomohiro Kubo, Rinka Sasaki, Toshiyuki Oda
Molecular Biology of the Cell 2024.5
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α- and β-tubulin C-terminal tails with distinct modifications are crucial for ciliary motility and assembly Reviewed Major achievement
Tomohiro Kubo, Yuma Tani , Haru-Aki Yanagisawa, Masahide Kikkawa, Toshiyuki Oda
Journal of Cell Science 2023.7
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Chlamydomonas FAP70 is a component of the previously uncharacterized ciliary central apparatus projection C2a Reviewed Major achievement
Yuqing Hou, Lei Zhao, Tomohiro Kubo, Xi Cheng, Nathan McNeill, Toshiyuki Oda, George B. Witman
JOURNAL OF CELL SCIENCE 2021.5( ISSN：0021-9533 )
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Diffusion rather than IFT likely provides most of the tubulin required for axonemal assembly Reviewed Major achievement
Julie Craft Van De Weghe, J. Aaron Harris, Tomohiro Kubo, George B. Witman, Karl F. Lechtreck
JOURNAL OF CELL SCIENCE 2020.8( ISSN：0021-9533 )
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TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii Reviewed Major achievement
Picariello T, Hou Y, Kubo T, McNeill NA, Yanagisawa HA, Oda T, Witman GB
PLoS One 2020.5( ISSN：1932-6203 )
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Chlamydomonas as a tool to study tubulin polyglutamylation. Invited Reviewed Major achievement
Kubo T and Oda T.
Microscopy 2018.10( ISSN：2050-5698 )
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CFAP70 is a Novel Axoneme-Binding Protein that Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Reviewed Major achievement
Shamoto N, Narita K, Kubo T, Oda T, and Takeda S.
Cells 2018.8( ISSN：2073-4409 )
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A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1). Reviewed Major achievement
MOLECULAR BIOLOGY OF THE CELL 2018.3( ISSN：1059-1524 )
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Electrostatic interaction between polyglutamylated tubulin and the nexin-dynein regulatory complex regulates flagellar motility. Reviewed Major achievement
Tomohiro Kubo and Toshiyuki Oda
MOLECULAR BIOLOGY OF THE CELL 2017.6( ISSN：1059-1524 )
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RABL2 interacts with the intraflagellar transport-B complex and CEP19 and participates in ciliary assembly. Reviewed Major achievement
Yuya Nishijima, Yohei Hagiya, Tomohiro Kubo, Ryota Takei, Yohei Katoh, and Kazuhisa Nakayama
MOLECULAR BIOLOGY OF THE CELL 28 ( 12 ) 1652 - 1666 2017.6( ISSN：1059-1524 )
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Together, the IFT81 and IFT74 N-termini form the main module for intraflagellar transport of tubulin. Reviewed Major achievement
JOURNAL OF CELL SCIENCE 129 ( 10 ) 2106 - 2119 2016.5( ISSN：0021-9533 )
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Reduced tubulin polyglutamylation suppresses flagellar shortness in Chlamydomonas. Reviewed Major achievement
MOLECULAR BIOLOGY OF THE CELL 26 ( 15 ) 2810 - 2822 2015.8( ISSN：1059-1524 )
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Assembly of IFT Trains at the Ciliary Base Depends on IFT74. Reviewed
CURRENT BIOLOGY 25 1583 - 1593 2015.6( ISSN：0960-9822 )
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A conserved flagella-associated protein in Chlamydomonas, FAP234, is essential for axonemal localization of tubulin polyglutamylase TTLL9. Reviewed Major achievement
MOLECULAR BIOLOGY OF THE CELL 25 107 - 117 2014.1( ISSN：1059-1524 )
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Tubulin polyglutamylation regulates flagellar motility by controlling a specific inner-arm dynein that interacts with the dynein regulatory complex. Reviewed Major achievement
Cytoskeleton 69 ( 12 ) 1059 - 1068 2012.12( ISSN：1949-3584 )
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Tubulin polyglutamylation regulates axonemal motility by modulating activities on inner-arm dyneins. Reviewed Major achievement
Current Biology 20 ( 5 ) 441 - 445 2010.3( ISSN：0960-9822 )
Books and Other Publications
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Regulation of eukaryotic flagellar motility by tubulin polyglutamylation
Tomohiro Kubo（ Role： Joint Work）
2020.3
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Regulation of eukaryotic flagellar motility by tubulin polyglutamylation
（ Role： Joint Work）
2020.1
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Regulation of eukaryotic flagellar motility by tubulin polyglutamylation Major achievement
Tomohiro Kubo（ Role： Joint Work）
2019.11
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Regulation of eukaryotic flagellar motility by tubulin polyglutamylation Major achievement
Tomohiro Kubo（ Role： Joint Work）
2019.10
Presentations
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Tubulin glycylation controls ciliary motility through modulation of outer-arm dyneins Major achievement
Tomohiro Kubo, Rinka Sasaki, and Toshiyuki Oda
2024.7
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クラミドモナスの鞭毛内で見つかった 蛋白質合成系の解明 Major achievement
久保智広
第13回繊毛研究会 2023.10
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Distinct roles of a- and b-tubulin C-terminal tails for ciliary function as revealed by a CRISPR/Cas9 mediated gene editing in Chlamydomonas Major achievement
Tomohiro Kubo, Yuma Tani, Haru-Aki Yanagisawa, Masahide Kikkawa, Toshiyuki Oda
2023.7
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単細胞緑藻類クラミドモナスの繊毛で新規に見つかった蛋白質合成系の解明 Major achievement
久保智広, 小田賢幸
第75回日本細胞生物学会 2023.6
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Distinct roles of a- and b-tubulin C-terminal tails for ciliary function as revealed by a CRISPR/Cas9 mediated gene editing in Chlamydomonas International conference Major achievement
Tomohiro Kubo, Yuma Tani, Haru-Aki Yanagisawa, Masahide Kikkawa, Toshiyuki Oda
20th International Conference on the Cell and Molecular Biology of Chlamydomonas 2023.6
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クラミドモナス鞭毛で新規に見つかった蛋白質合成系の解明 Major achievement
久保智広
繊毛研究会 2022.10
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SUnSET法による鞭毛構築中の新規合成蛋白質のモニタリング Major achievement
久保智広
第74回日本細胞生物学会 2022.6
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SUnSET法による鞭毛構築中の新規合成蛋白質のモニタリング Major achievement
久保智広
繊毛研究会 2021.11
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単細胞緑藻類クラミドモナスを用いた鞭毛内輸送系蛋白質IFT38の機能解析 Major achievement
久保智広
第73回日本細胞生物学会大会 2021.6
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チューブリンポリグルタミン酸化修飾による鞭毛繊毛の運動制御：αチューブリン改変株を用いた解析 Major achievement
久保智広
第14回クラミドモナス研究会 2021.3
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Ca2+ signaling surpasses the suppression of flagellar assembly in the axonemal dynein-lacking mutant Major achievement
Natsumi Kanou, Tomohiro Kubo1, and Toshiyuki Oda1
2020.9
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Identification of polyglutamylation sites in Chlamydomonas alpha-tubulin Major achievement
Tomohiro Kubo and Toshiyuki Oda1
2020.9
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カルシウムイオン依存的な鞭毛構築機構の解明
久保智広、加納夏実、小田賢幸
繊毛研究会 2019.11
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A Microtubule-Dynein Tethering Complex Regulates the Axonemal Inner Dynein f (I1) International conference Major achievement
Tomohiro Kubo
18th International Conference on the Cell and Molecular Biology of Chlamydomonas 2018.6
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A microtubule-dynein tethering complex regulates the axonemal inner dynein (I1)
Tomohiro Kubo, Yuqing Hou, Deborah Cochran, George Witman and Toshiyuki Oda
70th Annual Meeting of Japan Society for Cell Biology 2018.6
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The IFT81 and IFT74 N-termini together form the main module for intraflagellar transport(IFT) of tubulin Invited Major achievement
Tomohiro Kubo、Jason Brown, Karl Bellve, Branch Craige, Julie Craft, Kevin Forgarty, Karl Lechtreck, George Witman
2017.6
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Structural biology of Chlamydomonas flagella Invited Major achievement
Tomohiro Kubo and Toshiyuki Oda
2017.5
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The IFT81 and IFT74 N-termini together form the main module for intraflagellar transport(IFT) of tubulin Major achievement
Tomohiro Kubo, Jason Brown, Karl Bellve, Branch Craige, Julie Craft, Kevin Forgarty, Karl Lechtreck, George Witman
2016.11
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Axonemal tubulin polyglutamylation deficiency suppresses flagellar shortness Major achievement
2015.9
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The IFT81 tubulin-binding domain and the IFT74 N-terminus individually are dispensable but together are crucial for flagellar assembly International conference Major achievement
Tomohiro Kubo, Jason M. Brown, Branch Craige, and George B. Witman
FASEB (Biology of Cilia and Flagella) 2015.6
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A novel Chlamydomonas IFT81 mutant reveals that the IFT81 tubulin-binding domain is not crucial for tubulin transport and flagellar assembly International conference Major achievement
Tomohiro Kubo, Jason M. Brown, George B. Witman
The American Society for Cell Biology 2014 2014.12
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IFT74 is required for IFT-A/IFT-B interaction, but not for import of tubulin into flagella. International conference Major achievement
Jason M. Brown, Deborah A. Cochran, Tomohiro Kubo, and George B. Witman
16th International Conference on the Cell and Molecular Biology of Chlamydomonas 2014.6
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Axonemal tubulin polyglutamylation modulates flagellar length by enhancing tubulin turnover at the flagellar tip International conference Major achievement
Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, Ritsu Kamiya, and George B. Witman
16th International Conference on the Cell and Molecular Biology of Chlamydomonas 2014.6
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The N terminus of the IFT complex B protein IFT74 is required for normal flagellar import of complex A and BBS4 tracking, but not for tubulin import or flagellar assembly International conference Major achievement
Jason M. Brown, Deborah A. Cochran, Tomohiro Kubo, and George B. Witman.
The American Society for Cell Biology 2013 2013.12 The American Society for Cell Biology
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A suppressor mutant, ssh1, of flagellar shortness in dynein-deficient Chlamydomonas strains is deficient in the gene encoding FAP234, a protein involved in tubulin polyglutamylation International conference Major achievement
Tomohiro Kubo, Masafumi Hirono, Takumi Aikawa, George B. Witman, and Ritsu Kamiya
The American Society for Cell Biology 2013 2013.12 The American Society for Cell Biology
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Regulation of Axonemal Dynein Activity by a Conserved Tubulin Polyglutamylation System International conference Major achievement
Tomohiro Kubo, Zhongmei Liu, Takumi Aikawa, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya
Dynein 2013 International Workshop 2013.11
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Regulation of Axonemal Dynein Activity by a Conserved Tubulin Polyglutamylation System Major achievement
Tomohiro Kubo, Zhongmei Liu, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya
The 25th CDB Meeting; Cilia and Centrosomes: from Fertilization to Cancer 2013.6
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鞭毛運動のチューブリン・ポリグルタミン酸化修飾による調節 Major achievement
久保智広、八木俊樹、神谷律
生体運動班会議 2013.1
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A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein crucial for the localization of TTLL9, an enzyme that catalyzes tubulin polyglutamylation in the axoneme International conference Major achievement
omohiro Kubo, Yanagisawa Haru-aki, Zhongmei Liu, Rie Shibuya, Masafumi Hirono, and Ritsu Kamiya
The American Society for Cell Biology 2012 2012.12 The American Society for Cell Biology
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Tubulin polyglutamylation regulates axonemal motility by modulating the function of a particular inner-arm dynein species (dynein e) Major achievement
Tomohiro Kubo, Toshiki Yagi, and Ritsu Kamiya
2012.9
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Tubulin polyglutamylation regulates axonemal motility by modulating the function of a particular inner-arm dynein species (dynein e). International conference Major achievement
Tomohiro Kubo, Toshiki Yagi, and Ritsu Kamiya
15th International Conference on the Cell and Molecular Biology of Chlamydomonas 2012.6
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Tubulin polyglutamylation regulates flagellar motility by modulating the function of inner arm dynein “e” International conference Major achievement
Tomohiro Kubo, Toshiki Yagi, and Ritsu Kamiya
The American Society for Cell Biology 2011 2011.12 The American Society for Cell Biology
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軸糸チューブリン・ポリグルタミン酸化修飾に関与する新規鞭毛蛋白質の同定 Major achievement
久保智広、柳澤春明、劉中美、廣野雅文、神谷律
第81回日本動物学会 2011.9 日本動物学会
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Tubulin Polyglutamylation Regulates Flagellar Motility Through the Function of Dynein Regulatory Complex Major achievement
Tomohiro Kubo and Ritsu Kamiya
2011.9
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A novel Chlamydomonas mutant, tpg2, reveals a conserved 177-kDa protein associated with TTLL9, an enzyme that catalyzes tubulin polyglutamylation in the axoneme Major achievement
Tomohiro Kubo, Haru-aki Yanagisawa, Zhongmei Liu, Masafumi Hirono, and Ritsu Kamiya
2011.6
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チューブリン・ポリグルタミン酸化修飾による軸糸ダイニンと鞭毛運動の調節 Major achievement
久保智広、柳澤春明、八木俊樹、廣野雅文、神谷律
生体運動合同班会議 2010.11
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Tubulin Polyglutamylation Regulates Flagellar Motility by Affecting Functions of Inner-Arm Dyneins Major achievement
2010.9
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Tubulin Polyglutamylation Regulates Flagellar Motility by Affecting Functions of Inner-Arm Dyneins Major achievement
Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, Ritsu Kamiya
第62回日本細胞生物学会 2010.5 日本細胞生物学会
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Importance of Tubulin Polyglutamylation for Axonemal Dynein Function as Revealed by a Novel Chlamydomonas Mutant International conference Major achievement
Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, Ritsu Kamiya
2009.11
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クラミドモナス鞭毛運動におけるチューブリン・ポリグルタミル化修飾の重要性 Major achievement
久保智広、柳澤春明、八木俊樹、廣野雅文、神谷律
第61回日本細胞生物学会 2009.6 日本細胞生物学会
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Importance of tubulin polyglutamylation for cilia/flagella motility as revealed by a novel a Chlamydomonas mutant International conference Major achievement
Tomohiro Kubo, Haru-aki Yanagisawa, Toshiki Yagi, Masafumi Hirono, and Ritsu Kamiya
Gordon Research Conference 2009 (Cilia, Mucus & Mucociliary Interactions) 2009.2
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クラミドモナス変異株で明らかになった鞭毛チューブリン翻訳後修飾の機能的重要性 Major achievement
久保智広、柳澤春明、八木俊樹、神谷律
日本動物学会第79回大会 2008.9 日本動物学会
Awards
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Poster Award for Excellence Major achievement
2023.7
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16th International Conference on the Cell and Molecular Biology of Chlamydomonas. Poster award (1st place) Major achievement
2014.6 International Conference on the Cell and Molecular Biology of Chlamydomonas
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15th International Conference on the Cell and Molecular Biology of Chlamydomonas. Poster Award (3rd place) Major achievement
2012.6 International Conference on the Cell and Molecular Biology of Chlamydomonas
Teaching Experience (On-campus)
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Introduction to Human Morphology and Function Major achievement
2023Year
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Life Science of the Human Body
2023Year
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人体形態・機能学概論
2022Year Type of subject：Master's (Graduate School)
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全学共通教育科目「人体の生命科学」
2022Year Type of subject：Common education (undergraduate)
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解剖学A Major achievement
2019Year Type of subject：Common education (undergraduate)
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解剖学A
2018Year Type of subject：Common education (undergraduate)
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解剖学B
2018Year
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解剖学A Major achievement
2017Year Type of subject：Common education (undergraduate)
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分子組織学 Major achievement
2017Year Type of subject：Common education (undergraduate)
Guidance results
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2022
Type：Master's （Major B course）dissertations guidance Period：24months Year guidance time：500hours
Number of people receiving guidance ：1people
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2021
Type：Master's （Major B course）dissertations guidance Period：24months Year guidance time：500hours
Number of people receiving guidance ：1people
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2020
Type：Master's （Major B course）dissertations guidance Period：12months
Number of people receiving guidance ：1people
Teaching achievements
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FD研修会への参加（特進コース冬季合宿の際）
2019.02.21
Review of master's and doctoral thesis
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2022
Examiner classification：Second reader
Doctoral ：1people
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2021
Examiner classification：Second reader
Doctoral ：1people