Authorship：Last author,　Corresponding author   Language：English   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/s41467-021-21118-2.

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Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/bbb/zbab023

Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1038/s41598-019-42062-8

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Other Link： http://www.nature.com/articles/s41598-019-42062-8

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Membrane-associated RING-CH 8 (MARCH8) is one of 11 members of the MARCH family of RING finger E3 ubiquitin ligases and down-regulates several membrane proteins (e.g. major histocompatibility complex II [MHC-II], CD86, and transferrin receptor). We recently reported that MARCH8 also targets HIV-1 envelope glycoproteins and acts as an antiviral factor. However, it remains unclear whether other family members might have antiviral functions similar to those of MARCH8. Here we show that MARCH1 and MARCH2 are MARCH family members that reduce virion incorporation of envelope glycoproteins. Infectivity assays revealed that MARCH1 and MARCH2 dose-dependently suppress viral infection. Treatment with type I interferon enhanced endogenous expression levels of MARCH1 and MARCH2 in monocyte-derived macrophages. Expression of these proteins in virus-producing cells decreased the efficiency of viral entry and down-regulated HIV-1 envelope glycoproteins from the cell surface, resulting in reduced incorporation of envelope glycoproteins into virions, as observed in MARCH8 expression. With the demonstration that MARCH1 and MARCH2 are antiviral MARCH family members as presented here, these two proteins join a growing list of host factors that inhibit HIV-1 infection.

DOI： 10.1074/jbc.AC118.005907

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Language：English   Publishing type：(MISC) Introduction and explanation (others)   Publisher：Springer  

DOI： 10.1007/978-1-4939-8831-0_12

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DOI： 10.1038/s41598-018-33304-2

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier B.V.  

Transgenic animals provide attractive systems for the production of valuable recombinant proteins. Previous studies indicate that milk is a suitable source of secreted recombinant proteins. In the current study, we examine whether excrement can be another source of recombinant proteins by using transgenic mice ubiquitously-expressing green fluorescent protein (GFP) as a model. We found that the surface of excrement from GFP-transgenic mice was fluorescent, and the supernatant after centrifugation of an excrement suspension was rich in undegraded, actively fluorescing GFP. GFP was successfully purified from stool as a fluorescent 27 kDa protein by using a common procedure. Finally, we observed that the fluorescence of digested materials began in the ileum and persisted throughout the remainder of the digestive tract. Our results demonstrate that excrement, which is produced daily regardless of the sex or age of the animal, may be another feasible source of recombinant proteins. The preparation method is simple, economical, and noninvasive.

DOI： 10.1016/j.bbrc.2018.04.166

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Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PUBLISHING GROUP  

Inhibitors of Mek1/2 and Gsk3 beta, known as 2i, enhance the derivation of embryonic stem (ES) cells and promote ground-state pluripotency in rodents(1,2). Here we show that the derivation of female mouse ES cells in the presence of 2i and leukaemia inhibitory factor (2i/L ES cells) results in a widespread loss of DNA methylation, including a massive erasure of genomic imprints. Despite this global loss of DNA methylation, early-passage 2i/L ES cells efficiently differentiate into somatic cells, and this process requires genome-wide de novo DNA methylation. However, the majority of imprinting control regions (ICRs) remain unmethylated in 2i/L-ES-cell-derived differentiated cells. Consistently, 2i/L ES cells exhibit impaired autonomous embryonic and placental development by tetraploid embryo complementation or nuclear transplantation. We identified the derivation conditions of female ES cells that display 2i/L-ES-cell-like transcriptional signatures while preserving gamete-derived DNA methylation and autonomous developmental potential. Upon prolonged culture, however, female ES cells exhibited ICR demethylation regardless of culture conditions. Our results provide insights into the derivation of female ES cells reminiscent of the inner cell mass of preimplantation embryos.

DOI： 10.1038/nature23286

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Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

P0-Cre and Wnt1-Cre mouse lines have been widely used in combination with loxP-flanked mice to label and genetically modify neural crest (NC) cells and their derivatives. Wnt1-Cre has been regarded as the gold standard and there have been concerns about the specificity of P0-Cre because it is not clear about the timing and spatial distribution of the P0-Cre transgene in labeling NC cells at early embryonic stages. We re-visited P0-Cre and Wnt1-Cre models in the labeling of NC cells in early mouse embryos with a focus on cranial NC. We found that R26-lacZ Cre reporter responded to Cre activity more reliably than CAAG-lacZ Cre reporter during early embryogenesis. Cre immunosignals in P0-Cre and reporter (lacZ and RFP) activity in P0-Cre/R26-lacZ and P0-Cre/R26-RFP embryos was detected in the cranial NC and notochord regions in E8.0-9.5 (4-19 somites) embryos. P0-Cre transgene expression was observed in migrating NC cells and was more extensive in the forebrain and hindbrain but not apparent in the midbrain. Differences in the Cre distribution patterns of P0-Cre and Wnt1-Cre were profound in the midbrain and hindbrain regions, that is, extensive in the midbrain of Wnt1-Cre and in the hindbrain of P0-Cre embryos. The difference between P0-Cre and Wnt1-Cre in labeling cranial NC may provide a better explanation of the differential distributions of their NC derivatives and of the phenotypes caused by Cre-driven genetic modifications.

DOI： 10.1002/dvg.23034

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Language：English   Publishing type：Research paper (scientific journal)  

Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PUBLISHING GROUP  

Cloning animals by nuclear transfer provides the opportunity to preserve endangered mammalian species. However, there are risks associated with the collection of donor cells from the body such as accidental injury to or death of the animal. Here, we report the production of cloned mice from urine-derived cells collected noninvasively. Most of the urine-derived cells survived and were available as donors for nuclear transfer without any pretreatment. After nuclear transfer, 38-77% of the reconstructed embryos developed to the morula/blastocyst, in which the cell numbers in the inner cell mass and trophectoderm were similar to those of controls. Male and female cloned mice were delivered from cloned embryos transferred to recipient females, and these cloned animals grew to adulthood and delivered pups naturally when mated with each other. The results suggest that these cloned mice had normal fertility. In additional experiments, 26 nuclear transfer embryonic stem cell lines were established from 108 cloned blastocysts derived from four mouse strains including inbreds and F1 hybrids with relatively high success rates. Thus, cells derived from urine, which can be collected noninvasively, may be used in the rescue of endangered mammalian species by using nuclear transfer without causing injury to the animal.

DOI： 10.1038/srep23808

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Authorship：Corresponding author   Language：Japanese   Publishing type：(MISC) Introduction and explanation (scientific journal)  

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Language：English   Publishing type：(MISC) Introduction and explanation (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English  

DOI： 10.1016/j.bbagrm.2014.05.020

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATL ACAD SCIENCES  

In mammals, VDJ recombination is responsible for the establishment of a highly diversified preimmune antibody repertoire. Acquisition of a functional Ig heavy (H) chain variable (V) gene rearrangement is thought to prevent further recombination at the IgH locus. Here, we describe V(H)Q52(NT); V kappa gr32(NT) Ig monoclonal mice reprogrammed from the nucleus of an intestinal IgA(+) plasma cell. In V(H)Q52(NT) mice, IgA replaced IgM to drive early B-cell development and peripheral B-cell maturation. In V(H)Q52(NT) animals, over 20% of mature B cells disrupted the single productive, non-autoimmune IgH rearrangement through VH replacement and exchanged it with a highly diversified pool of IgH specificities. VH replacement occurred in early pro-B cells, was independent of pre-B-cell receptor signaling, and involved predominantly one adjacent VH germ-line gene. VH replacement was also identified in 5% of peripheral B cells of mice inheriting a different productive VH rearrangement expressed in the form of an IgM H chain. In summary, editing of a productive IgH rearrangement through VH replacement can account for up to 20% of the IgH repertoire expressed by mature B cells.

DOI： 10.1073/pnas.1417988112

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE  

During organogenesis, Sonic hedgehog (Shh) possesses dual functions: Shh emanating from midline structures regulates the positioning of bilateral structures at early stages, whereas organ-specific Shh locally regulates organ morphogenesis at later stages. The mesonephros is a transient embryonic kidney in amniote, whereas it becomes definitive adult kidney in some anamniotes. Thus, elucidating the regulation of mesonephros formation has important implications for our understanding of kidney development and evolution. In Shh knockout (KO) mutant mice, the mesonephros was displaced towards the midline and ectopic mesonephric tubules (MTs) were present in the caudal mesonephros. Mesonephros-specific ablation of Shh in Hoxb7-Cre;Shh(flox/-) and Sall1(CreERT2/+);shh(flox/-) mice embryos indicated that Shh expressed in the mesonephros was not required for either the development of the mesonephros or the differentiation of the male reproductive tract. Moreover, stage-specific ablation of Shh in Shh(CreERr2/flox) mice showed that notochord- and/or floor plate-derived Shh were essential for the regulation of the number and position of MTs. Lineage analysis of hedgehog (Hh)-responsive cells, and analysis of gene expression in Shh MO embryos suggested that Shh regulated nephrogenic gene expression indirectly, possibly through effects on the paraxial mesoderm. These data demonstrate the essential role of midline-derived Shh in local tissue morphogenesis and differentiation. (C) 2013 The Authors. Published by Elsevier Inc. All rights reserved.

DOI： 10.1016/j.ydbio.2013.12.026

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Authorship：Corresponding author   Language：English   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：John Wiley and Sons Ltd  

Background: A postovulatory mammalian oocyte decreases developmental potential with in vivo aging in the oviduct or in vitro aging in the culture dish. The mechanism underlying oocyte aging still largely remains an enigma. Accumulating data suggest that the epigenetic alterations such as histone acetylation are also associated with postovulatory aging. Objective: To perform a review evaluating a new aspect of oocyte aging in terms of the epigenetic alterations focusing on lysine acetylation. Methods: In addition to a search of the literature in Pubmed, we introduced our recent published data. Results: Histone acetylation in the mouse oocyte increases during aging, potentially impacting gene regulation in the subsequent embryonic development. Oocyte aging results in increased acetylation of alpha-tubulin, a non-histone protein, and nicotinamide, an inhibitor of class III HDAC, partially prevents some of oocyte aging phenotypes. Conclusion: Abnormal regulation of protein acetylation itself is suggested in oocyte aging and could contribute to the aging phenotypes. © 2013 Japan Society for Reproductive Medicine.

DOI： 10.1007/s12522-013-0172-y

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：Humana Press Inc.  

In situ hybridization is a powerful method for detecting endogenous mRNA sequences in morphologically preserved samples. We provide in situ hybridization methods, which are specifically optimized for mouse embryonic samples as whole mounts and section tissues. Additionally, β-Galactosidase (β-gal) is a popular reporter for detecting the expression of endogenous or exogenous genes. We reveal that 6-chloro-3-indoxyl-β-d-galactopyranoside (S-gal) is a more sensitive substrate for β-gal activity than 5-bromo-4-chloro-3-indolyl- β-d-galactoside (X-gal). S-gal is advantageous where β-gal activity is limited including early stage mouse embryos. As a result of the increased sensitivity as well as the color compatibility of S-gal, we successfully combined β-gal staining using S-gal with in situ hybridization using DIG-labeled probes in both whole mounts and sections. © 2014 Springer Science+Business Media, New York.

DOI： 10.1007/978-1-60327-292-6_1

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Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

Postovulatory mammalian oocyte developmental potential decreases with aging in vivo and in vitro. Aging oocytes typically show cellular fragmentation and chromosome scattering with an abnormally shaped spindle over time. Previously, it was shown that histone acetylation in the mouse oocyte increased during aging and that treatment with trichostatin A (TSA), an inhibitor for class I and II histone deacetylases (HDACs), enhanced the acetylation, that is, aging. In this study, we examined the effect of nicotinamide (NAM), an inhibitor for class III HDACs, on in vitro aging of mouse oocytes as well as TSA. We found that treatment with NAM significantly inhibited cellular fragmentation, spindle elongation and astral microtubules up to 48 h of culture. Although presence of TSA partially inhibited cellular fragmentation and spindle elongation up to 36 h of culture, treatment with TSA induced chromosome scattering at 24 h of culture and more severe cellular fragmentation at 48 h of culture. Further, we found that a-tubulin, a nonhistone protein, increased acetylation during aging, suggesting that not only histone but nonhistone protein acetylation may also increase with oocyte aging. Thus, these data indicate that protein acetylation is abnormally regulated in aging oocytes, which are associated with a variety of aging phenotypes, and that class I/II and class III HDACs may play distinct roles in aging oocytes.

DOI： 10.1262/jrd.2012-171

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ACADEMIC PRESS INC ELSEVIER SCIENCE  

Previous studies show that treatment of zygotes with trichostatin A (TSA), a histone deacetylase inhibitor (HDACi), impacts the subsequent development to a blastocyst as well as full-term development. To reveal the dynamics of protein acetylation, with and without TSA treatment during one-cell stage, we examined oocytes and zygotes by immunofluorescence and Western Blot analyses using anti-acetylated lysine and acetylated alpha-tubulin antibodies. In unfertilized oocytes, lysine acetylation level was extremely low over all but faintly detected in the spindle. Once oocyte activation occurs, a dramatic increase of lysine acetylation signal was observed mostly in the pronuclei and a fiber-like structure, the so called midbody, suggesting activation coupled up-regulation of lysine acetylation presumably in histones and alpha-tubulin. TSA treatment resulted in significantly more hyperacetylation not only in the midbody structure and pronuclei but also in the whole cytoplasm. Consistently, Western Blot analysis revealed that acetylation of proteins about 53 kDa and 11 kDa in size, corresponding to alpha-tubulin and histone H4 sizes respectively, were increased mainly after oocyte activation and exclusively enhanced by TSA treatment in zygotes. To confirm this behavior of acetylated nonhistone proteins, acetylated alpha-tubulin was examined and found to be faintly detected in the spindle of MII oocytes but later in whole in the cell of zygotes including the midbody, which was enhanced by TSA treatment. To elucidate the mechanism underlying up-regulation of lysine acetylation following oocyte activation, we assayed the HDAC activity, and found significant reduction of HDAC activity from MII to zygotic stages. Taken together, our data indicate that HDACs play an important role in maintaining low acetylated status in a MII oocyte. However, once an oocyte has been activated, histone and nonhistone proteins including alpha-tubulin are hyperacetylated partly due to a reduction of HDAC activity. TSA treatment of zygotes enhances their acetylation, which could affect subsequent embryonic development. (C) 2013 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2013.03.083

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：CELL PRESS  

Previous studies of serial cloning in animals showed a decrease in efficiency over repeated iterations and a failure in all species after a few generations. This limitation led to the suggestion that repeated recloning might be inherently impossible because of the accumulation of lethal genetic or epigenetic abnormalities. However, we have now succeeded in carrying out repeated recloning in the mouse through a somatic cell nuclear transfer method that includes a histone deacetylase inhibitor. The cloning efficiency did not decrease over 25 generations, and, to date, we have obtained more than 500 viable offspring from a single original donor mouse. The reprogramming efficiency also did not increase over repeated rounds of nuclear transfer, and we did not see the accumulation of reprogramming errors or clone-specific abnormalities. Therefore, our results show that repeated iterative recloning is possible and suggest that, with adequately efficient techniques, it may be possible to reclone animals indefinitely.

DOI： 10.1016/j.stem.2013.01.005

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：(MISC) Introduction and explanation (scientific journal)   Publisher：Humana Press Inc.  

In mammals, a diploid genome following fertilization of haploid cells, an egg, and a spermatozoon is unique and irreproducible. This implies that the generated unique diploid genome is doomed with the individual's inevitable demise. Since it was first reported in 1997 that Dolly the sheep had been cloned, many mammalian species have been cloned successfully using somatic-cell nuclear transfer (SCNT). The success of SCNT in mammals enables us not only to reproduce offspring without germ cells, that is, to "passage" a unique diploid genome, but also to address valuable biological questions on development, nuclear reprogramming, and epigenetic memory. Successful cloning can also support epigenetic reprogramming where the aging clock is reset or reversed. Recent work using iPS cell technology has explored the practicality and led to the recapitulation of premature aging with iPSCs from progeroid laminopathies. As a result, reprogramming tools are also expected to contribute to studying biological age. However, the efficiency of animal cloning is still low in most cases and the mechanism of reprogramming in cloned embryos is still largely unclear. Here, based on recent advances, we describe an improved, more efficient mouse cloning protocol using histone deacetylase inhibitors (HDACis) and latrunculin A, which increases the success rates of producing cloned mice or establishing ES cells fivefold. This improved method of cloning will provide a strong tool to address many issues including biological aging more easily and with lower cost. © Springer Science+Business Media, New York 2013.

DOI： 10.1007/978-1-62703-556-9_9

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：MARY ANN LIEBERT INC  

Cloning mammals by somatic cell nuclear transfer (SCNT) has become an established procedure, but the success rate remains low and gene expression abnormalities are also observed. In addition, SCNT pups exhibited an abnormal gene expression profile with a high degree of heterogeneity among individuals. Recently, we reported that somatic clones treated with trichostatin A (TSA) exhibited a significantly improved success rate, probably due to its effects on chromatin remodeling and histone modification in early embryos. Here we show that the TSA treatment also improves the long-term consistency of genome-wide gene expression regulation: the total number of genes commonly exhibiting up- or downregulation in the TSA clone pups decreased to half of the conventional SCNT pups, and the variation among individuals observed in the SCNT pups was also reduced to the level of the pups produced by the intracytoplasmic sperm injection (ICSI) method. Interestingly, the total gene expression profile of the TSA Clones came to resemble that of the ICSI pups.

DOI： 10.1089/cell.2011.0062

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Event date： 2024.10

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：秋田市にぎわい交流館AU（あう）   Country：Japan  

Event date： 2024.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：名古屋大学 野依記念学術交流館   Country：Japan  

Event date： 2024.6

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：国際医療福祉大学東京赤坂キャンパス（東京）   Country：Japan  

Event date： 2023.12

Language：Japanese   Presentation type：Poster presentation  

Venue：神戸ポートアイランド （神戸国際会議場、神戸国際展示場、神戸ポートピアホテル）   Country：Japan  

Event date： 2023.11

Language：Japanese   Presentation type：Symposium workshop panel(nominated)  

Venue：石川県立音楽堂、金沢   Country：Japan  

Event date： 2023.11

Language：English   Presentation type：Oral presentation(general)  

Venue：大阪府立国際会議場、大阪   Country：Japan  

Event date： 2023.10 - 2023.11

Language：Japanese   Presentation type：Poster presentation  

Venue：福岡国際会議場・マリンメッセ福岡   Country：Japan  

Event date： 2023.10 - 2023.11

Language：Japanese   Presentation type：Poster presentation  

Venue：福岡国際会議場・マリンメッセ福岡   Country：Japan  

Event date： 2023.10 - 2023.11

Language：Japanese   Presentation type：Poster presentation  

Venue：福岡国際会議場・マリンメッセ福岡   Country：Japan  

Event date： 2023.10

Language：English   Presentation type：Oral presentation(general)  

Venue：University of Malaya, Kuala Lumpur, Malaysia   Country：Malaysia  

Event date： 2023.9

Language：English   Presentation type：Oral presentation(general)  

Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：神戸大学　農学部学舎   Country：Japan  

Event date： 2023.9

Language：English   Presentation type：Oral presentation(general)  

Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：神戸大学　農学部学舎   Country：Japan  

Event date： 2023.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：神戸大学　農学部学舎   Country：Japan  

Event date： 2023.9

Language：English   Presentation type：Oral presentation(general)  

Country：Japan  

Event date： 2023.7

Language：English   Presentation type：Poster presentation  

Venue：Shaw Centre, Ottawa   Country：Canada  

Event date： 2023.7

Language：English   Presentation type：Poster presentation  

Venue：Shaw Centre, Ottawa   Country：Canada  

Event date： 2022.11

Language：Japanese   Presentation type：Poster presentation  

Venue：パシフィコ横浜会議センター  

Event date： 2022.10

Language：Japanese   Presentation type：Poster presentation  

Venue：艮陵（ごんりょう）会館　仙台市  

Event date： 2022.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学　農学部弥生講堂  

Event date： 2022.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学　農学部弥生講堂  

Event date： 2022.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学　農学部弥生講堂  

Event date： 2022.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学　農学部弥生講堂  

Event date： 2022.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学　農学部弥生講堂  

Event date： 2022.5

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：メルパルク京都(京都)  

Event date： 2021.9

Language：Japanese   Presentation type：Poster presentation  

Venue：京都大学農学部総合館  

Event date： 2021.9

Language：Japanese   Presentation type：Poster presentation  

Venue：京都大学農学部総合館  

Event date： 2021.9

Language：Japanese   Presentation type：Poster presentation  

Venue：京都大学農学部総合館  

Event date： 2021.9

Language：Japanese   Presentation type：Poster presentation  

Venue：京都大学農学部総合館  

Event date： 2021.9

Language：Japanese   Presentation type：Poster presentation  

Venue：京都大学農学部総合館  

Event date： 2021.8

Language：Japanese   Presentation type：Public discourse, seminar, tutorial, course, lecture and others  

Venue：県立和歌山医科大学  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東北大学の予定（オンラインに変更）  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東北大学の予定（オンラインに変更）  

Event date： 2020.9

Language：Japanese   Presentation type：Poster presentation  

Venue：東北大学の予定（オンラインに変更）  

Event date： 2020.7

Language：English   Presentation type：Poster presentation  

Event date： 2020.7

Language：English   Presentation type：Poster presentation  

Venue：カナダの予定（オンラインに変更）  

Event date： 2020.7

Language：English   Presentation type：Poster presentation  

Event date： 2020.1

Language：English   Presentation type：Poster presentation  

Event date： 2020.1

Language：English   Presentation type：Poster presentation  

Event date： 2019.10

Language：English   Presentation type：Oral presentation(invited, special)  

Event date： 2019.10

Language：English   Presentation type：Poster presentation  

Event date： 2019.10

Language：English   Presentation type：Poster presentation  

Event date： 2019.10

Language：English   Presentation type：Poster presentation  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：北海道大学  

Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation(general)  

Venue：北海道大学  

Event date： 2019.9

Language：Japanese   Presentation type：Poster presentation  

Venue：北海道大学  

Event date： 2019.9

Language：Japanese   Presentation type：Poster presentation  

Venue：北海道大学  

Event date： 2019.9

Language：Japanese   Presentation type：Poster presentation  

Venue：北海道大学  

Event date： 2019.9

Language：Japanese   Presentation type：Poster presentation  

Venue：北海道大学  

Event date： 2019.9

Language：Japanese   Presentation type：Poster presentation  

Venue：北海道大学  

Event date： 2018.9

Language：Japanese   Presentation type：Poster presentation  

Venue：信州大学上田キャンパス  

Event date： 2018.9

Language：Japanese   Presentation type：Poster presentation  

Venue：信州大学上田キャンパス  

Event date： 2018.9

Language：Japanese   Presentation type：Poster presentation  

Venue：信州大学上田キャンパス  

Event date： 2018.9

Language：Japanese   Presentation type：Poster presentation  

Venue：信州大学上田キャンパス  

Event date： 2018.9

Language：Japanese   Presentation type：Poster presentation  

Venue：信州大学上田キャンパス  

Event date： 2018.6

Language：English   Presentation type：Poster presentation  

Event date： 2018.6

Language：English   Presentation type：Poster presentation  

Event date： 2017.9

Language：English   Presentation type：Poster presentation  

Event date： 2017.9

Language：English   Presentation type：Poster presentation  

Event date： 2017.9

Language：English   Presentation type：Poster presentation  

Event date： 2016.9

Language：Japanese  

Event date： 2016.9

Language：Japanese   Presentation type：Oral presentation(invited, special)  

Event date： 2016.9

Language：Japanese