Updated on 2024/06/30

写真a

 
Onuki Yoshitugu
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Life and Environmental Sciences Bioagronomy (Biotechnology) Assistant Professor
Title
Assistant Professor

Degree

  • 博士(医工学) ( 山梨大学 )

  • 修士(工学) ( 山梨大学 )

Research Interests

  • 胚様体

  • 培養工学

  • ヒトiPS細胞

  • 多能性幹細胞

Research Projects

  • ヒトiPS細胞の革新的な非凍結密閉系保存技術の開発

    2021.4 - 2024.3

Papers

  • Optimized conditions for the supplementation of human-induced pluripotent stem cell cultures with a GSK-3 inhibitor during embryoid body formation with the aim of inducing differentiation into mesodermal and cardiac lineage.

    Kiyomi Yoda, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   2020.3( ISSN:1389-1723 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions. Reviewed

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   2018.10( ISSN:1389-1723 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • ROCK INHIBITOR Y-27632 USED DURING CELL AGGREGATE FORMATION ADVERSELY AFFECTS THE EARLY CARDIAC DIFFERENTIATION OF HUMAN iPS CELLS Reviewed

        36 ( 2 )   21 - 29   2017.5( ISSN:1881-3704 )

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.11418/jtca.36.21

  • ヒトiPS細胞の分化誘導プロセスにおける細胞の構造構築段階の重要性 Reviewed

    大貫喜嗣, 黒澤尋

    THE CHEMICL TIMES   2016.7( ISSN:02852446 )

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  • Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation. Reviewed

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   111 ( 1 )   92 - 97   2011.1( ISSN:1389-1723 )

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    Language:English   Publishing type:Research paper (scientific journal)  

Presentations

  • 保存培地のpH がヒトiPS 細胞の新規非凍結保存安定性に及ぼす影響

    佐藤 佑菜, 徐 卓然, 大貫 喜嗣, 田中 佑治, 黒澤 尋

    第74回日本生物工学会大会  2022.10 

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation(general)  

  • Primitive Endoderm Cells Promote Proliferation And Differentiation Of Pluripotent Stem Cells International conference

    Takumi Iwama, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa

    Japanese Association for Animal Cell technology  2020.11 

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    Event date: 2020.11

    Language:English   Presentation type:Oral presentation(general)  

  • Screening Of Molecules That Facilitate Transition From Mouse Embryonic Stem Cells To Early-Embryonic-Like Cells International conference

    Aoi Murakami, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa

    Japanese Association for Animal Cell technology  2020.11 

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    Event date: 2020.11

    Language:English   Presentation type:Oral presentation(general)  

  • Novel Cyclic N-alkyl Peptides as BFGF Mimetics to Maintain the Pluripotency of Human Induced Pluripotent Stem Cells International conference

    Masashi Sato, Hiromi Tanaka, Yoshitsugu Ohnuki, Shinji Masui, Takashi Kawakami, Hiroshi Kurosawa

    Japanese Association for Animal Cell technology  2020.11 

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    Event date: 2020.11

    Language:English   Presentation type:Oral presentation(general)  

  • 細胞低接着性コラーゲンがヒトiPS細胞の未分化性および増殖性に及ぼす作用

    大貫 喜嗣, 米澤 優希, 升井 伸治, 國井 沙織, 平岡 陽介, 森本 康一, 黒澤 尋

    第19回日本再生医療学会総会  2020.3 

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    Event date: 2020.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:パシフィコ横浜  

  • ヒトiPS細胞の未分化増殖を可能にする新規ペプチド化合物の分子進化工学的スクリーニング法による探索及び人工FGFRアゴニストとしての機能解析

    佐藤 将, 大貫 喜嗣, 升井 伸治, 川上 隆史, 黒澤 尋

    第71回日本生物工学会大会  2019.9 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:岡山大学  

  • GSK-3β inhibitor treatment for human iPS cells to form mesoderm differentiationpromoted embryoid bodies

    The 92nd Annual Meeting of Japanese Tissue Culture Association  2019.7 

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    Event date: 2019.7

    Language:Japanese   Presentation type:Oral presentation(general)  

  • 細胞低接着性コラーゲンが未分化維持培養におけるヒトiPS細胞の形態および未分化性に及ぼす影響

    米澤 優希, 大貫 喜嗣, 國井 沙織, 伊田 寛之, 平岡 陽介, 森本 康一, 黒澤 尋

    第18回日本再生医療学会総会  2019.3  日本再生医療学会

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    Event date: 2019.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:神戸国際展示場 2号館1F コンベンションホール北  

  • 胚様体形成期間中のCHIR99021処理濃度がヒトiPS細胞の心筋細胞分化誘導に与える影響

    依田 聖未, 大貫 喜嗣, 黒澤 尋

    第70回 日本生物工学会大会  2018.9  日本生物工学会

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation(general)  

    Venue:関西大学 千里山キャンパス  

  • Variation in the expression levels of cardiac differentiation-related genes during inducing cardiomyocytes from human iPS cells.

    Minami Kasai, Miku Sawanobori, Yoshitsugu Ohnuki, Tomoya Sawaguchi, Takehiro Watanabe, Hiroyuki Yamaguchi, Hiroshi Kurosawa

    2018.6 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Poster presentation  

  • 細胞低接着性コラーゲン培養により形成するES/iPS細胞の胚様体の解析

    森本康一,國井沙織,伊田寛之,平岡陽介,大貫喜嗣

    日本再生医療学会  2018.3 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Poster presentation  

  • New approach to forming embryoid bodies of ES cells by using Low Adhesive Scaffold Collagen. International conference

    S.Kunii, Y. Ohnuki, K. Morimoto

    2017 ASCB | EMBO Meeting  2017.12 

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    Event date: 2017.12

    Language:English   Presentation type:Poster presentation  

  • 細胞低接着型コラーゲンを用いた多能性幹細胞からの接着性凝集体の形成と分化への影響

    入江真純、國井沙織、伊田寛之、大貫喜嗣、黒澤尋、平岡陽介、森本康一

    第5回細胞凝集研究会  2017.11 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Poster presentation  

  • 胚様体形成段階におけるGSK3β阻害剤添加が ヒトiPS細胞の心筋細胞分化に及ぼす影響

    澤登 美空, 大貫 喜嗣, 黒澤 尋

    第69回日本生物工学会大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Poster presentation  

  • Effect of CHIR99021 on the proliferation and differentiation status of human iPS cells in the maintenance culture

    2017.6 

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    Event date: 2017.6

    Language:Japanese   Presentation type:Oral presentation(general)  

  • ヒトiPS細胞由来脂肪細胞を用いた没食子酸エピガロカテキンの機能性評価

    ○清水 康子1, 大貫 喜嗣1, 河野 高徳2, 卯川 裕一2, 提坂 裕子2, 望月 和樹1, 黒澤 尋1

    第68回日本生物工学会大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

  • ノコダゾール処理によるヒトiPS細胞の心筋分化誘導

    ○山口 千尋, 大貫 喜嗣, 黒澤 尋

    第68回日本生物工学会大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

  • 維持培養および胚様体形成期間中の培地がヒトiPS細胞の心筋分化誘導に与える影響

    第68回日本生物工学会大会  2016.9  日本生物工学会

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

  • Effect of Y-27632 concentration on EB formation and differentiation into cardiomyocytes from human iPS cells

    2016.5 

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    Event date: 2016.5

    Language:Japanese   Presentation type:Poster presentation  

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The total number of announcement other than the above

  • 2016

    Total number of non-preannounced events:1  Number of main presenters:1  Number of presenters other than main person:0

Awards

  • 奨励賞

    2017.7   日本組織培養学会   CHIR99021が維持培養期間中のヒトiPS細胞の増殖性および分化方向性に及ぼす影響

    大貫 喜嗣

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    Award type:Award from Japanese society, conference, symposium, etc. 

Teaching Experience (On-campus)

  • Biochemical Engineering Major achievement

    2023Year

  • 教職履修カルテ

    2023Year

  • Research on Bioscience A

    2023Year

  • Seminar on Bioscience A

    2023Year

  • 教職履修カルテ

    2017Year  Type of subject:Professional education (undergraduate)

  • 研究発表B

    2017Year  Type of subject:Professional education (undergraduate)

  • 研究発表A

    2017Year  Type of subject:Professional education (undergraduate)

  • 生命を科学する

    2017Year  Type of subject:Common education (undergraduate)

  • 細胞培養工学

    2017Year  Type of subject:Professional education (undergraduate)

  • バイオサイエンス研究B

    2017Year  Type of subject:Professional education (undergraduate)

  • バイオサイエンス演習B

    2017Year  Type of subject:Professional education (undergraduate)

  • 生命研究倫理学

    2017Year  Type of subject:Professional education (undergraduate)

  • 生物工学実験II

    2017Year  Type of subject:Professional education (undergraduate)

  • バイオサイエンス研究A

    2017Year  Type of subject:Professional education (undergraduate)

  • バイオサイエンス演習A

    2017Year

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Guidance results

  • 2022

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :3people 

  • 2022

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :2people 

  • 2021

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :5people 

  • 2021

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :4people 

  • 2020

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :3people  (Overseas students):1people

  • 2020

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :4people 

  • 2019

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :4people 

  • 2019

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :3people 

  • 2018

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :2people 

    Graduation / pass / number of people awarded degrees :2people 

    Number of teachers:2people

  • 2018

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :5people 

    Graduation / pass / number of people awarded degrees :5people 

    Number of teachers:2people

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Teaching achievements

  • 第1回学域FD研修会 「全国ならびに山梨大学におけるデータサイエンス教育の現状と課題」

    2022.12.01

  • 融合研究に培われる先進的大学院教育の実践

    2021.12.17

  • 生命工学科FD 「大学教職員向け 新課程入試情報セミナー
      ~ステークホルダーから共感され、効果を実感できる入試改革を考える~

    2021.12.15

  • 生命環境学域第1回FD研修会「学生のSOSをどう受け止めるか」

    2021.08.04

Other educational achievements

  • 生物工学実験IIにて実習用のテキストを作成した。
    日本の標準的な動物細胞培養の方法について学べるように実習内容を改めた。
    発生工学研究および創薬分野の基礎となるヒトiPS細胞からの心筋分化誘導を実施した。
    また、動画による反転授業を行い高評価を得た。

    2018

Review of master's and doctoral thesis

  • 2022

    Examiner classification:Second reader

    Master :5people 

  • 2021

    Examiner classification:Second reader

    Master :5people 

Social Activities

  • わかりやすい再生医療

    愛知県立豊丘高等学校  2021.10

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    Audience: High school students

    Type:Visiting lecture

  • 動物細胞のDNAを見てみよう

    Role(s): Lecturer

    県立日川高等学校  サイエンスラボⅢ  2021.10

  • わかりやすい再生医療

    山梨県立韮崎高等学校  2021.9

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    Audience: High school students

    Type:Visiting lecture

  • わかりやすい再生医療

    山梨県立豊田南高等学校  2020.11

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    Audience: High school students

    Type:Visiting lecture

  • わかりやすい再生医療

    山梨県立豊田南高等学校  2019.11

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    Audience: High school students

    Type:Visiting lecture

  • わかりやすい再生医療

    山梨県立巨摩高等学校  2019.11

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    Audience: High school students

    Type:Visiting lecture

  • わかりやすい再生医療

    山梨県立韮崎高等学校  2019.9

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    Audience: High school students

    Type:Visiting lecture

  • 動物細胞のDNAを見てみよう

    Role(s): Lecturer

    県立日川高等学校  サイエンスラボⅢ  2019.8

  • わかりやすい再生医療

    愛知県立豊田南高校  2018.11

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    出前授業での講演

  • わかりやすい再生医療

    県立韮崎高等学校  SSHアドバンス講座  2018.11

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    出前授業での講演

  • 動物細胞のDNAを見てみよう

    Role(s): Lecturer

    県立日川高等学校  サイエンスラボⅢ  2018.8

  • わかりやすい再生医療

    県立韮崎高等学校  県立韮崎高等学校  2017.10

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    出前授業での講演

  • 動物細胞のDNAを見てみよう

    Role(s): Lecturer

    県立日川高等学校  2017.8

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