Updated on 2024/06/30

写真a

 
Takashi Ishiuchi
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Life and Environmental Sciences Bioagronomy (Biotechnology) Associate Professor
Title
Associate Professor

Research Projects

  • 染色体除去・可視化・顕微操作による染色体操作法の確立

    2024.1 - 2025.4

    上原記念生命科学財団  研究助成金

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    Authorship:Principal investigator  Grant type:Competitive  Type of fund::Donation

  • ヒストンH3.3による受精後エピゲノムリプログラミングの意義の解明

    2023.12 - 2025.9

    公益財団法人内藤記念科学振興財団  内藤記念科学奨励金・研究助成 

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    Grant type:Competitive  Type of fund::Donation

  • 卵子の恒常性維持およびその破綻に寄与するエピゲノム変化の解明

    2023.12 - 2024.12

    公益財団法人持田記念医学薬学振興財団  持田記念研究助成金 

  • 多能性の多階層的制御機構の解明

    2023.11 - 2026.3

    公益財団法人第一三共生命科学研究振興財団 

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    Authorship:Principal investigator 

  • 多能性の多階層制御機構の解明

    2023.11 - 2024.11

    住友財団  基礎科学研究助成

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    Authorship:Principal investigator  Grant type:Competitive  Type of fund::Donation

  • 卵子形成・維持に資する遺伝子群の網羅的同定とその分子機能の解明

    2023.6 - 2028.3

    公益財団法人 武田科学振興財団  ビジョナリーリサーチ助成 (スタート) 

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    Grant type:Competitive  Type of fund::Donation

  • 全能性プログラムにおけるエピゲノム再編成の理解とその人為的制御

    2019.6 - 2024.3

    新学術領域研究

  • 全能性プログラムにおけるエピゲノム再編成の理解とその人為的制御

    2019.6 - 2024.3

    新学術領域研究

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    Grant type:Competitive 

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Papers

  • YY1-dependent transcriptional regulation manifests at the morula stage Reviewed

    microPublication Biology   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Molecular mechanisms underlying totipotency Reviewed

    Life Science Alliance   2023.9

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Dynamic nucleosome remodeling mediated by YY1 underlies early mouse development Reviewed

    GENES & DEVELOPMENT   2023.8( ISSN:0890-9369 )

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Paternally inherited H3K27me3 affects chromatin accessibility in mouse embryos produced by round spermatid injection Reviewed

    DEVELOPMENT   2022.9( ISSN:0950-1991 )

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Histone H3K36me2 and H3K36me3 form a chromatin platform essential for DNMT3A-dependent DNA methylation in mouse oocytes Reviewed

    Seiichi Yano, Takashi Ishiuchi, Shusaku Abe, Satoshi H. Namekawa, Gang Huang, Yoshihiro Ogawa & Hiroyuki Sasaki

    Nature Communications   13 ( 1 )   4440 - 4440   2022.8( ISSN:2041-1723 )

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Establishment of the DNA methylation landscape of mammalian oocytes, mediated by the DNMT3A-DNMT3L complex, is crucial for reproduction and development. In mouse oocytes, high levels of DNA methylation occur exclusively in the transcriptionally active regions, with moderate to low levels of methylation in other regions. Histone H3K36me3 mediates the high levels of methylation in the transcribed regions; however, it is unknown which histone mark guides the methylation in the other regions. Here, we show that, in mouse oocytes, H3K36me2 is highly enriched in the X chromosome and is broadly distributed across all autosomes. Upon H3K36me2 depletion, DNA methylation in moderately methylated regions is selectively affected, and a methylation pattern unique to the X chromosome is switched to an autosome-like pattern. Furthermore, we find that simultaneous depletion of H3K36me2 and H3K36me3 results in global hypomethylation, comparable to that of DNMT3A depletion. Therefore, the two histone marks jointly provide the chromatin platform essential for guiding DNMT3A-dependent DNA methylation in mouse oocytes.

    DOI: 10.1038/s41467-022-32141-2

    PubMed

  • Healthy cloned offspring derived from freeze-dried somatic cells Reviewed

    Wakayama S, Ito D, Hayashi E, Ishiuchi T, Wakayama T.

    Nature Communications   2022.7

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    Language:English   Publishing type:Research paper (scientific journal)  

  • TFB2M and POLRMT are essential for mammalian mitochondrial DNA replication Reviewed

    Teppei Inatomi, Shigeru Matsuda, Takashi Ishiuchi, Yura Do, Masunari Nakayama, Shusaku Abe, Kazutoshi Kasho, Sjoerd Wanrooij, Kazuto Nakada, Kenji Ichiyanagi, Hiroyuki Sasaki, Takehiro Yasukawa, Dongchon Kang

    BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH   2022.1( ISSN:0167-4889 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • A histone H3.3K36M mutation in mice causes an imbalance of histone modifications and defects in chondrocyte differentiation Reviewed

    Epigenetics   2021.10( ISSN:1559-2294 )

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Reprogramming of the histone H3.3 landscape in the early mouse embryo Reviewed

    Takashi Ishiuchi, Shusaku Abe, Kimiko Inoue, Wan Kin Au Yeung, Yuka Miki, Atsuo Ogura, Hiroyuki Sasaki

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   2021.1( ISSN:1545-9993 )

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • Zfp281 Shapes the Transcriptome of Trophoblast Stem Cells and Is Essential for Placental Development Reviewed

    Cell Reports   2019.5( ISSN:2211-1247 )

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  • A molecular roadmap for the emergence of early-embryonic-like cells in culture Reviewed

    NATURE GENETICS   2018.1( ISSN:1061-4036 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • CAMSAP3 orients the apical-to-basal polarity of microtubule arrays in epithelial cells Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   2016.1( ISSN:0027-8424 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Early embryonic-like cells are induced by downregulating replication-dependent chromatin assembly Reviewed

    NATURE STRUCTURAL & MOLECULAR BIOLOGY   2015.9( ISSN:1545-9993 )

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • LINEing germ and embryonic stem cells' silencing of retrotransposons Reviewed

    GENES & DEVELOPMENT   2014.7( ISSN:0890-9369 )

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • Higher chromatin mobility supports totipotency and precedes pluripotency in vivo Reviewed

    GENES & DEVELOPMENT   2014.5( ISSN:0890-9369 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Towards an understanding of the regulatory mechanisms of totipotency Reviewed

    CURRENT OPINION IN GENETICS & DEVELOPMENT   2013.10( ISSN:0959-437X )

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • Nectins localize Willin to cell-cell junctions Reviewed

    GENES TO CELLS   2012.5( ISSN:1356-9597 )

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • Mechanosensitive EPLIN-dependent remodeling of adherens junctions regulates epithelial reshaping Reviewed

    JOURNAL OF CELL BIOLOGY   2011.8( ISSN:0021-9525 )

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    Language:English   Publishing type:Research paper (scientific journal)  

  • Willin and Par3 cooperatively regulate epithelial apical constriction through aPKC-mediated ROCK phosphorylation Reviewed

    NATURE CELL BIOLOGY   2011.6( ISSN:1465-7392 )

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  • Mammalian Fat and Dachsous cadherins regulate apical membrane organization in the embryonic cerebral cortex Reviewed

    JOURNAL OF CELL BIOLOGY   2009.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

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Books and Other Publications

  • 初期胚における遺伝子発現・転写制御

    ( Role: Joint Work)

    医学書院  2023.6  ISSN:0370-9531

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    Language:Japanese   Book type:Textbook, survey, introduction

Presentations

  • 全能性を支えるエピゲノム・転写リプログラミング

    全能性プログラム 第5回公開シンポジウム  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Symposium workshop panel(nominated)  

  • Nucleosome organization in the early mouse embryos International conference

    The International Symposium “Totipotency and Germ Cell Development”  2022.11 

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    Event date: 2022.11

    Language:English   Presentation type:Oral presentation(invited, special)  

  • Nucleosome organization in the early mouse embryos International conference

    The International Symposium “Totipotency and Germ Cell Development”  2022.11 

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    Language:English   Presentation type:Oral presentation(invited, special)  

  • 受精後発生における転写ダイナミクス

    第74回日本細胞生物学会大会  2022.6 

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    Event date: 2022.6

    Language:Japanese   Presentation type:Symposium workshop panel(nominated)  

  • 受精後発生における転写ダイナミクス

    第74回日本細胞生物学会大会  2022.6 

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    Language:Japanese   Presentation type:Symposium workshop panel(nominated)  

  • 受精後発生を支えるエピゲノム・転写ネットワーク解明にむけた試み Invited

    バイオDX キックオフシンポジウム  2021.12 

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    Event date: 2021.12

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • マウス初期発生における転写ダイナミクス Invited

    2021年度幹細胞研究会  2021.12  新学術領域研究「植物多能性幹細胞」

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    Event date: 2021.12

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:オンライン  

  • 受精後発生における転写ダイナミクス

    第44回日本分子生物学会年会  2021.12 

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    Event date: 2021.12

    Language:Japanese   Presentation type:Symposium workshop panel(nominated)  

  • マウス初期発生における転写ダイナミクス Invited

    2021年度幹細胞研究会  2021.12  新学術領域研究「植物多能性幹細胞」

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    Language:Japanese   Presentation type:Oral presentation(invited, special)  

    Venue:オンライン  

  • 受精後発生を支えるエピゲノム・転写ネットワーク解明にむけた試み Invited

    バイオDX キックオフシンポジウム  2021.12 

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    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • 受精後発生における転写ダイナミクス

    第44回日本分子生物学会年会  2021.12 

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    Language:Japanese   Presentation type:Symposium workshop panel(nominated)  

  • 受精卵に秘められる謎を紐解く ―ゲノム、エピゲノム、遺伝子発現制御の観点から― Invited

    第52回 発生工学研究センターセミナー 発生工学技術開発・実践特別教育プログラム (山梨大学)  2021.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • 受精卵に秘められる謎を紐解く ―ゲノム、エピゲノム、遺伝子発現制御の観点から― Invited

    第52回 発生工学研究センターセミナー 発生工学技術開発・実践特別教育プログラム (山梨大学)  2021.9 

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    Language:Japanese   Presentation type:Oral presentation(invited, special)  

  • eprogramming of the histone H3.3 landscape in the early mouse embryo International conference

    2020.12 

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    Event date: 2020.12

    Language:English   Presentation type:Symposium workshop panel(nominated)  

  • Reprogramming of the histone H3.3 landscape in the early mouse embryo International conference

    第43回 日本分子生物学会  2020.12 

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    Language:English   Presentation type:Symposium workshop panel(nominated)  

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Awards

  • 日本エピジェネティクス研究会奨励賞

    2021.3   日本エピジェネティクス研究会   哺乳類の初期発生の基盤となるエピゲノム制御機構

    石内崇士

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    Award type:Award from Japanese society, conference, symposium, etc. 

  • 日本エピジェネティクス研究会奨励賞

    2021.3   日本エピジェネティクス研究会   哺乳類の初期発生の基盤となるエピゲノム制御機構

    石内崇士

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    Award type:Award from Japanese society, conference, symposium, etc. 

Teaching Experience (On-campus)

  • Basic Data Analysis in Life Science and Medical Research Major achievement

    2023Year

  • Advanced Lecture on Biological Data Sciences Major achievement

    2023Year

  • Seminar in Life and Environmental Sciences

    2023Year

  • Seminar on Bioscience A

    2023Year

  • Research on Bioscience A

    2023Year

Guidance results

  • 2023

    Type:Ph.D. dissertations guidance

    Number of people receiving guidance :2people 

  • 2023

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :3people 

  • 2023

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :3people 

  • 2022

    Type:Ph.D. dissertations guidance

    Number of people receiving guidance :1people 

  • 2022

    Type:Master's (Major B course)dissertations guidance

    Number of people receiving guidance :1people 

  • 2022

    Type:Undergraduate (Major A course)graduation thesis guidance

    Number of people receiving guidance :3people 

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Review of master's and doctoral thesis

  • 2023

    Examiner classification:Second reader

    Master :2people 

    Doctoral :2people 

  • 2022

    Examiner classification:Second reader

    Master :3people  (Overseas student):0people

    Doctoral :1people  (Overseas student):0people

    Thesis Dr. :0people  (Overseas student):0people

  • 2022

    Examiner classification:Chief examiner

    Master :1people  (Overseas student):0people

    Doctoral :0people  (Overseas student):0people

    Thesis Dr. :0people  (Overseas student):0people