Language：English   Publishing type：Research paper (scientific journal)  

The Warthin tumor is a benign neoplasm of the salivary glands, histologically, the tumor has an oncocytic epithelial component forming uniform rows of cells surrounded by cystic spaces associated with a lymphoid stroma often showing the presence of germinal centers. The lymphoid stroma is a representative microscopic finding. If this lymphocytic accumulation is active, some sort of transmitter should exist between the Warthin tumor cells and lymphocytes. C-X-C motif chemokine ligand (CXCL12) 12, CXCL10 and C-C motif chemokine ligand 18 (CCL18) are a chemoattractant for lymphocytes in vivo. There is no report on the relationship between these chemokines and Warthin tumors. In this study, we investigated these chemokines expressions in 20 Warthin tumors using immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). For comparison, we also enrolled samples of pleomorphic adenoma, which is another benign salivary gland tumor type without prominent lymphocytic infiltration. All Warthin tumors were immunopositive for CXCL12 and CXCL10, and these reactivities were diffuse. Meanwhile, the majority of pleomorphic adenomas were immunonegative for CXCL12 (95%), CXCL10 (80%) and CCL18 (85%). Warthin tumor and pleomorphic adenoma cases were significantly different in these immunostaining expressions (CXCL12, p<0.001; CXCL10, p<0.001; CCL18, p=0.024). We examined CXCL12, CXCL10 and CCL18 mRNA expressions of 3 representative Warthin tumor samples, each having these chemokines immunopositive areas detected by RT-PCR. Finding CXCL12 and CXCL10 expressions indicate that these chemokines may play a part in the formation of a lymphoid stroma within Warthin tumors. In regards to this phenomenon, the participation of CCL18 might be restrictive compared to CXCL12 and CXCL10.

DOI： 10.14670/HH-18-352

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

In endometrioid carcinomas (ECs) of the uterine corpus, neutrophil accumulation within the carcinoma cell clusters is a representative microscopic finding. Because this accumulation is active, some sort of transmitter ought to exist between the EC cells and neutrophils. Interleukin-8 (IL-8) and C-X-C motif chemokine ligand 5 (CXCL5) is a cytokine that attracts neutrophils in vivo. In this study, we investigated IL-8, CXCL5 and C-X-C motif chemokine receptor 2 (CXCR2) (their chemokine receptor) expressions in ECs by immunohistochemistry and reverse transcription polymerase chain reaction (RT-PCR). There are few reports on the relationship between these chemokines and ECs. For comparison, we enrolled samples of colorectal adenocarcinoma (CRAC), it is another representative tumor with neutrophil infiltration. We analyzed 30 ECs and 30 CRACs. We confirmed IL-8 expression (H-score ≥50 points) in 40% of EC and 7% of CRAC samples; CXCL5 expression in 7% of EC and 10% of CRAC samples; CXCR2 expression in 83% of EC and 53% of CRAC samples by immunohistochemistry. We examined each mRNA (IL-8 and CXCL5) expression of 3 representative EC and 3 CRAC samples. Finding IL-8 expression might indicate that this cytokine is important for the process of neutrophil accumulation, particularly within ECs. The participation of CXCL5 regarding neutrophil accumulation within their carcinoma cell clusters might be restrictive compared to IL-8.

DOI： 10.14670/HH-18-281

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

DOI： 10.1111/pin.12937

Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/pin.12937

Language：English   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

AIMS: CD10 is an endopeptidase that degrades various bioactive peptides in the extracellular matrix. In addition to enzymatic degradation, it affects multiple intracellular signal transduction pathways. CD10 expression has been extensively studied in human epithelial cancers of numerous organs and sites. However, its presence in thyroid carcinomas, especially in anaplastic thyroid carcinoma (ATC), has not been fully determined. An actual CD10 expression in thyroid lesions including a large series of ATC was evaluated. METHODS AND RESULTS: We examined CD10 by immunohistochemistry (IHC) in 152 thyroid lesions: nine adenomatous goitres (AGs) and 143 tumours, including 47 anaplastic carcinomas. IHC showed diffuse and strong positivity for CD10 in the epithelial components of almost all ATCs. However, epithelia with squamous metaplasia and oncocytic change from AGs, follicular adenomas and differentiated carcinomas had focal CD10 reactivity. Some papillary thyroid carcinomas (PTCs), along with the PTC components of some ATCs, showed CD10 positivity in fibroblast-like stromal cells and fibrous material. CONCLUSION: Our results imply that the CD10 expression pattern depended on the histotypes of thyroid lesions. When possible metastatic tumours and non-epithelial tumours are excluded, high CD10 expression may be useful in determining whether a primary thyroid carcinoma includes an anaplastic component.

DOI： 10.1111/his.13657

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：International Institute of Anticancer Research  

Background/Aim: The main objective of the present study was to evaluate the significance of apoptosis in the Fletcher’s risk classification for gastrointestinal stromal tumors (GISTs). Materials and Methods: Apoptotic cells were identified by immunostaining for single-stranded DNA (ssDNA). We assigned each GIST to one of four risk groups: very low risk, n=32
low risk, n=53
intermediate risk, n=15
high risk, n=6). Results: The mean ssDNA labeling index for each group was 8.0±44.2, 20.1±86.5, 18.7±38.6 and 5.7±5.7, respectively. Fletcher’s risk classification for GISTs correlated significantly with the ssDNA labeling index (p=0.002). Conclusion: The ssDNA labeling index and Ki-67 labeling index were the most significant factors corresponding to the risk grade of GISTs. These findings suggest that the ssDNA labeling index might be useful for predicting aggressive biological behavior of GISTs.

DOI： 10.21873/anticanres.12542

Scopus

PubMed

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier Ltd  

Introduction: Gastrointestinal duplication cyst is a congenital rare disease that may occur in any region from mouth to anus. Among them, gastric duplication cysts are very rare. Case report: Here we report A 23-year-old Japanese man who visited our hospital to evaluate an abdominal tumor. Abdominal computed tomography showed a well-circumscribed homogenous low-density mass measuring 6.2 × 6.0 cm between the pancreatic tail and the upper posterior wall on the gastric greater curvature, and the mass seemed to originate from the pancreatic tail. We found intraoperatively that the mass adhered to the stomach and pancreatic tail strongly, so we performed laparoscopic partial gastrectomy and spleen-preserving distal pancreatectomy. Pathological findings showed that the lining epithelium of the cystic mass consisted of the gastric foveolar epithelium with fundic glands. Furthermore, the pancreatic tissue of the pancreatic tail and the muscular layer of the cystic mass were intermingled. Discussion: GDCs are usually diagnosed at a younger age and in adults, they are very rare. Therefore, surgical resection is considered to be the best treatment due to the difficulty of diagnosis, and also that it mimics a pancreatic cystic tumor, and malignant transformation. Complete resection of the cyst is the ideal technique and laparoscopic surgery should be selected whenever possible. Conclusion: We experienced a case of GDC continuous to both stomach and pancreatic tail. Laparoscopic surgery is safety and useful even if GDC is continuous with both the stomach and the pancreas.

DOI： 10.1016/j.ijscr.2018.02.028

Scopus

Language：English   Publishing type：Research paper (scientific journal)   Publisher：INT INST ANTICANCER RESEARCH  

Background/Aim: Although differentiating squamous cell carcinoma (SCC) from adenocarcinoma (AC) at the esophagogastric junction (EGJ) is important for the choice of treatment, this can occasionally be difficult with small biopsy specimens. Therefore, the purpose of this study was to determine the most useful immunomarker panel for discriminating between SCC and AC of the EGJ. Materials and Methods: We analyzed 15 SCCs and 26 ACs of the EGJ obtained surgically using immunohistochemistry. Results: The sensitivities of p40, p63 and cytokeratin 5/6 were 100% with specificities of 88%, 46% and 81%, respectively, for SCC. The sensitivities of CAM5.2, caudal-type homeobox 2 (CDX2), mucin-5AC (MUC-5AC) and MUC-6 were 100%, 81%, 77% and 85% with specificities of 27%, 100%, 87% and 87% for AC. Conclusion: We demonstrated that a two-marker panel of p40 and CDX2 is highly sensitive and specific.

DOI： 10.21873/anticanres.12147

Web of Science

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：HUMANA PRESS INC  

Pediatric papillary thyroid carcinoma (PTC) has unique features but requires further genetic investigation. Moreover, there has been increasing concern about the risk for pediatric PTC in Japan after the Fukushima accident. This study aims to evaluate the frequencies of BRAF and TERT promoter mutations and to examine their significance in non-radiation-associated pediatric PTCs in Japan. We enrolled 81 pediatric PTC patients aged &lt;= 20 years. The control group included 91 adult PTCs from patients &gt; 20 years old. BRAF and TERT mutations were analyzed by allele-specific-PCR and/or Sanger sequencing. Compared with adult PTCs, pediatric PTCs exhibited larger tumor size, more frequent lymph node metastasis, and less classical histology. The prevalence of BRAF(V600E) in pediatric PTCs was 54% and significantly lower than that in adults of 85%. In the pediatric PTCs, BRAF(V600E) was positively associated with older age, classical histology, and the lymph node metastasis but independent from other clinicopathological factors. TERT mutations were identified in 13% of adults and in none of the pediatric PTCs. In conclusion, pediatric PTCs are characterized by more advanced clinicopathological features, lower BRAF(V600E) frequency, and absence of TERT mutation. The BRAF(V600E) frequency in this study is similar to the reported BRAF(V600E) frequency in the ultrasonographically screened pediatric PTCs in Fukushima.

DOI： 10.1007/s12022-017-9470-y

Web of Science

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Authorship：Lead author   Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：日本内分泌外科学会・日本甲状腺外科学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

BRAF V600E mutation, RET rearrangements, and RAS mutations are the common genetic alterations in differentiated thyroid carcinomas derived from follicular thyroid cells. However, the relationship between these alterations and iodine intake is still controversial. To clarify the influence of iodine intake on the occurrence of differentiated thyroid carcinomas, we performed molecular analyses for two differentiated carcinomas, papillary thyroid carcinomas (PTCs) and follicular thyroid carcinomas (FTCs), from an iodine-rich country (Japan) and an iodine-deficient country (Vietnam). We examined 120 PTCs (67 Japanese and 53 Vietnamese) and 74 FTCs (51 Japanese and 23 Vietnamese). We carried out allele-specific polymerase chain reaction (AS-PCR) for BRAF V600E, PCR and direct sequencing for RAS mutations (codon 12, 13, and 61 in NRAS, HRAS, and KRAS), and RT-PCR for RET/PTC1 and RET/PTC3. BRAF V600E was present in 55/67 (82.1%) Japanese PTCs and 44/53 (83%) Vietnamese PTCs. RET/PTC1 was identified in only one PTC from each country, and no samples had RET/PTC3. NRAS mutation was found in 17/51 (33.3%) Japanese FTCs and 4/23 (17.4%) Vietnamese FTCs. NRAS mutation was cited in codon 61 (20 cases) and codon 12 (one case). None of FTCs had KRAS or HRAS mutations. There were no significant differences in the prevalence of BRAF V600E, RET/PTC, or RAS mutations between the two countries. Our study showed no differences in genetic alterations of thyroid cancers from iodine-rich and iodine-deficient countries, possibly suggesting that iodine intake might not affect the genetic alterations of differentiated thyroid cancer.

DOI： 10.1002/cam4.781

Web of Science

Authorship：Lead author   Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：日本病理学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：W B SAUNDERS CO-ELSEVIER INC  

The NRAS(A182G) mutation, which results in the NRAS(Q61R) protein, is a major driver mutation in follicular-patterned thyroid neoplasms. Although new immunohistochemistry (IHC) for NRAS(Q61R) is now available, its sensitivity, specificity, and diagnostic utility for thyroid tumors are not yet established. We performed IHC for NRAS(Q61R) and direct sequencing for NRAS codon 61 in 4 thyroid cancer derived cell lines and 98 follicular-patterned thyroid tumors that included 22 follicular thyroid adenomas (FTAs), 35 follicular thyroid carcinomas (FTCs), and 41 cases of nodular hyperplasia (NH). In the tumors with NRAS(Q61R), the expression of BRAF(V600E) was further evaluated immunohistochemically. Two cell lines with NRAS(A182G) showed selective immunoreactivity for NRAS(Q61R). In tumor tissues, NRAS(Q61R) IHC was positive in 18% (4/22), 29% (10/35), and 2% (1/41) of FTAs, FTCs, and NH samples, respectively. The frequencies of the NRASQ61R in FTAs and FTCs were significantly higher than that in NH (P = .046 and P = .001, respectively). All tumors with NRAS(Q61R) expression exhibited uniform cytoplasmic positivity with or without accumulation in their cell membranes. Of the 15 tumors with NRAS(Q61R) expression, 13 cases showed NRAS(A182G) in direct sequencing, whereas all of the tumors without NRAS(Q61R) expression were negative for the mutation. There were no tumors with overlapping expression of NRAS(Q61R) and BRAF(V600E). In reference to the direct sequencing, sensitivity and specificity of the NRAS(Q61R) IHC were 100% and 98%, respectively. In conclusion, NRAS(Q61R) IHC is a highly sensitive and specific tool that is useful for differentiating follicular-patterned thyroid tumors. (C) 2016 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.humpath.2016.02.008

Web of Science

Language：English   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Authorship：Lead author   Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PUBLISHING GROUP  

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：NATURE PUBLISHING GROUP  

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

We present a case of spindle cell oncocytoma (SCO) of the adenohypophysis in a 70-year-old Vietnamese male. The patient was admitted to Cho Ray Hospital after suffering from headache and visual disturbance for 6 months. Clinicians detected a 60 x 55 x 45 mm(3) mass located in the suprasellar-sellar region. Histopathologically, the resected tumor was composed of spindle cells with oncocytic appearance. Immunohistochemical examination revealed expression of anti -mitochondria antibody (AMA), vimentin, thyroid transcription factor 1 (ITF-1), epithelial membrane antigen (EMA) and galectin-3. These histologic and immunohistochemical findings are suggestive of SCO. (C) 2015 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.prp.2015.07.014

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：LIPPINCOTT WILLIAMS & WILKINS  

Branchial cleft cysts (BCCs) are also named lateral cervical cysts and widely acknowledged as being derived from embryonic remnants. Lymphoepithelial cysts (LECs) generally show microscopic features that are identical to those of BCCs, and rarely occur at unusual sites or organs.
A case of multiple cysts arising in both lobes of the thyroid gland, thymus, and right parotid gland in a 41-year-old man is reported. Clinically, the patient presented with Hashimoto's thyroiditis for about 20 years and had past histories of idiopathic thrombocytopenic purpura and severe respiratory infection.
This case is unusual in that multiple cysts arose synchronously and/or heterochronously and grew, increasing their sizes in these different organs. Microscopic examinations revealed that all of the cysts were composed of squamous epithelium, dense lymphoid tissue with germinal centers, and a fibrous capsule. These findings corresponded to those of BCCs or LECs. It is notable that the histopathological features were nearly the same in the individual organs. A review of the literature disclosed no previous such reported cases.
The etiology is unknown. However, based upon the similar histopathological features of all the excised specimens, common immune and/or hematopoietic disorders may have contributed to their occurrence and development in association with putative genetic abnormalities.

DOI： 10.1097/MD.0000000000001758

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

Papillary thyroid carcinomas (PTCs) occasionally form multiple tumor foci in different sites of the same thyroid gland. However, it is controversial whether discrete nodules of PTC arise independently (multicentric occurrence) or are seeded from a single tumor via lymphatic channels (intraglandular metastasis). In order to determine the clonal origin of multiple PTCs, we examined X-chromosome inactivation patterns using a human androgen receptor gene-based assay (HUMARA) and the BRAF mutation using allele-specific PCR (AS-PCR) in 32 microdissected cancerous tissues from 14 Japanese women with multifocal PTC. All tumor foci were greater than 3 mm in size and met the criteria for microscopic classical PTC. Samples from 13 of the 14 patients were informative based on HUMARA. Tumor foci from two cases (15.4%) displayed a discordant X-chromosome inactivation pattern. Foci from the other 11 cases (84.6%) showed a concordant inactivation pattern of the X-chromosome. AS-PCR indicated that BRAF mutational status between the tumor foci was discordant in three (25%) and concordant in nine (75%) of 12 available cases. When the results of these two molecular analyses were combined, 28.6% of the cases were discordant in X-chromosome inactivation pattern and/or BRAF mutation, suggesting multicentric origin. Some of the remaining concordant cases also may be of multicentric origin. These results support a hypothesis that multicentric occurrence in multiple PTCs may be common, possibly greater than 30%. Although the exact mechanism of multicentric occurrence is still unclear, our findings contribute to the understanding the histogenesis of papillary thyroid carcinoma.

DOI： 10.1002/cam4.466

Web of Science

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY  

Paired-box gene 8 (PAX8)-peroxisome proliferator-activated receptor- (PPAR) gene fusion has been identified at significant frequency in follicular thyroid carcinomas (FTCs) with cytogenetically detectable translocation t(2;3)(q13;p25). This represents a possible specific molecular marker for follicular carcinoma. In this study, we examined PAX8-PPAR rearrangement in 24 FTC samples from Japanese patients by reverse transcribed-polymerase chain reaction (RT-PCR) using two upstream PAX8 primers located in exons 7 and 8 and a downstream primer in exon 1 of PPAR. The fusion gene was detected in only one of 24 FTCs (4%). The FTC with PAX8-PPAR rearrangement from a 56-year-old man showed a product consistent with fusion between exon 8 of PAX8 and exon 1 of PPAR. It was confirmed by direct sequencing. This FTC was histologically encapsulated, composed of trabeculae and small follicles and had complete penetration of the capsule by tumor tissues (minimally invasive type). The frequency of the fusion gene in this study was much lower than the 29-63% noted in reports from other countries suggesting that FTCs in Japanese patients may have a special genetic background, and that the high iodine intake from a typical Japanese diet might influence the frequency of the fusion gene in FTCs.

DOI： 10.1111/pin.12270

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

DOI： 10.1111/pin.12241

Web of Science

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：(MISC) Summary of the papers read (international conference)   Publisher：NATURE PUBLISHING GROUP  

Web of Science

Language：English   Publishing type：(MISC) Summary of the papers read (international conference)   Publisher：NATURE PUBLISHING GROUP  

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

Little is known about the squamous morular component (SMC) in colorectal neoplasms because of its rarity. The aim of the present study is to elucidate the morphological, immunohistochemical and genetic characteristics of SMCs in colorectal adenomas. Five colorectal adenomas having SMCs were resected from five patients endoscopically. On immunohistochemical examination (four cases), all SMCs were positive for cytokeratin 5/6 in their cytoplasm and positive for P-catenin in their cytoplasm and nuclei. A nuclear positivity of p63 was detected in one SMC. All SMCs were negative for p53, chromogranin A, synaptophysin and NCAM. There was no Ki-67 expression in any of the SMCs. We detected none of mutations of beta-catenin, KRAS and BRAFby microdissection and polymerase chain reaction-direct sequence in any of the four examined SMCs. SMCs are a rare but problematic finding in colorectal adenomas. Using immunohistochemistry for beta-catenin, cytokeratin 5/6, Ki-67, p53, chromogranin A, synaptophysin and NCAM can facilitate the diagnosis of these peculiar cell nests. (C) 2015 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.prp.2015.05.002

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

We describe a case of signet ring cell carcinoma of the non-ampullary duodenum in an 86-year-old woman. Endoscopic examination revealed a fungating lesion (Borrmann classification; type 2) on the anterior wall of the descending duodenum (second part). Pylorus-preserving pancreaticoduodenectomy and lymphadenectomy were performed. The tumor (pT3 and pN0) was predominantly composed of signet ring cells, characterized by a central, optically clear, globoid droplet of cytoplasmic mucin with an eccentrically placed nucleus. The cells were positive for periodic acid Schiff with diastase and Alcian blue pH 2.5. On immunohistochemical examination, MUC-5AC, MUC-2 and CDX2 were diffusely positive, and MUC-6 was negative in the tumor cells, which consisted of mixed gastric foveolar and intestinal phenotypes. Cytokeratin 7 was focally positive, but cytokeratin 20 was negative. Nuclear staining of p53 was diffusely and weakly positive. Signet ring cell carcinoma occurring in the non-ampullary duodenum might derive from duodenal goblet cells with MUC-5AC expression. (C) 2015 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.prp.2015.04.013

Web of Science

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(公社)日本臨床細胞学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：W B SAUNDERS CO-ELSEVIER INC  

We report a case of localized Langerhans cell histiocytosis characterized by clonal aggregation of Langerhans cells in the thymus and identified with molecular genetic study. A 43-year-old Japanese woman was found to have an anterior mediastinal mass by radiologic studies. Laparoscopy-assisted biopsy was subsequently performed. Histologically, we found subtle nodules scattered in the thymus consisting of aggregated Langerhans cells, which caused destruction of Hassall corpuscles. These Langerhans cells were immunohistochemically positive for S-100, CD1a, and CD207/langerin. Using allele-specific polymerase chain reaction and immunohistochemistry with mutation-specific antibody VE1, the BRAF V600E mutation was identified in aggregated Langerhans cells. At the medical follow-up, the thymic tumor had spontaneously regressed; however, identification of oncogenic BRAF mutation supports the neoplastic nature of the current case. (C) 2014 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.humpath.2013.12.018

Web of Science

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER GMBH, URBAN & FISCHER VERLAG  

Thyroid-like low-grade nasopharyngeal papillary adenocarcinoma (TL-LGNPPA) is an extremely rare neoplasm characterized by morphological analogy to papillary thyroid carcinoma and abnormal expression of thyroid transcription factor-1 (TTF-1). Here we report a novel case of TL-LGNPPA with literature review. The patient was a 43-year-old woman complaining of nasal obstruction. Laryngoscopic study and computed tomography identified a pedunculated mass located on the posterior edge of the left nasal septum. Histologically, the tumor consisted of papillary growth of cuboidal or columnar epithelium. Tubular architecture and a spindle cell component were also observed focally. Some tumor cells exhibited intra-nuclear cytoplasmic inclusions. Immunohistochemically, the neoplastic cells were positive for pancytokeratin (AE1/AE3), CK7, CK19, TTF-1, vimentin and HBME1, but negative for thyroglobulin, Pax8 and CK5/6. Ki67-labeling index reached 5% in the most concentrated spot. Despite the morphological and immunohistochemical similarity to papillary thyroid carcinoma, no BRAF V600E mutation was detected by mutation-specific immunohistochemistry. The patient had neither local recurrence nor distant metastasis 19 months after removal of the tumor. (C) 2014 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.prp.2014.04.020

Web of Science

Language：Japanese   Publishing type：(MISC) Summary of the papers read (national conference and other science council)   Publisher：(一社)日本病理学会  

Language：English   Publishing type：Research paper (scientific journal)   Publisher：LIPPINCOTT WILLIAMS & WILKINS  

Objective-Apolipoprotein (apo) A-II is the second major apo of high-density lipoproteins, yet its pathophysiological roles in the development of atherosclerosis remain unknown. We aimed to examine whether apo A-II plays any role in atherogenesis and, if so, to elucidate the mechanism involved.
Methods and Results-We compared the susceptibility of human apo A-II transgenic (Tg) rabbits to cholesterol diet-induced atherosclerosis with non-Tg littermate rabbits. Tg rabbits developed significantly less aortic and coronary atherosclerosis than their non-Tg littermates, while total plasma cholesterol levels were similar. Atherosclerotic lesions of Tg rabbits were characterized by reduced macrophages and smooth muscle cells, and apo A-II immunoreactive proteins were frequently detected in the lesions. Tg rabbits exhibited low levels of plasma C-reactive protein and blood leukocytes compared with non-Tg rabbits, and high-density lipoproteins of Tg rabbit plasma exerted stronger cholesterol efflux activity and inhibitory effects on the inflammatory cytokine expression by macrophages in vitro than high-density lipoproteins isolated from non-Tg rabbits. In addition, beta-very-low-density lipoproteins of Tg rabbits were less sensitive to copper-induced oxidation than beta-very-low-density lipoproteins of non-Tg rabbits.
Conclusion-These results suggest that enrichment of apo A-II in high-density lipoprotein particles has atheroprotective effects and apo A-II may become a target for the treatment of atherosclerosis. (Arterioscler Thromb Vasc Biol. 2013; 33: 224-231.)

DOI： 10.1161/ATVBAHA.112.300445

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：ELSEVIER IRELAND LTD  

Objective: Excess intake of a high-fat diet (HFD) is associated with obesity and metabolic syndrome, which are major risk factors for cardiovascular disease. However, it is unclear whether consumption of an HFD at a normal calorific range would be detrimental to metabolism or affect the development of atherosclerosis. Here, we tested the hypothesis that consumption of a normal-calorie HFD would impair lipid metabolism, insulin sensitivity, and blood pressure.
Methods and results: Rabbits fed with an HFD containing either 3% (15% kcal from fat) or 10% (25.8% kcal from fat) coconut oil were compared with control rabbits fed with a standard chow diet (9.3% kcal from fat). All rabbits consumed an equal amount of calories of their respective food. However, HFD feeding induced marked metabolic disorders including increased plasma levels of free fatty acids, insulin resistance, and hypertension compared with control rabbits. Metabolic disorders were more pronounced in 10%-HFD-fed rabbits than 3%-HFD-fed rabbits. To examine whether these disorders affected the development of atherosclerosis, two HFD groups were further fed with a diet containing 0.3% cholesterol for another 18 weeks. We found that 10%-HFD group showed a prominent accumulation of adipose tissue and developed 2-fold greater aortic atherosclerosis than 3%-HFD group.
Conclusions: These results suggest that consuming an HFD containing even a normal number of calories can cause insulin resistance, hypertension, and adipose accumulation even without obesity. High amounts of fat in diets apparently accelerate the development of atherosclerosis. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

DOI： 10.1016/j.atherosclerosis.2010.07.051

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Language：Japanese   Presentation type：Poster presentation  

Language：Japanese   Presentation type：Poster presentation  

Language：Japanese   Presentation type：Oral presentation(general)  

Language：Japanese   Presentation type：Oral presentation(general)  

Language：Japanese   Presentation type：Poster presentation