CLEC-2 stimulates IGF-1 secretion from podoplanin-positive stromal cells and positively regulates erythropoiesis in mice Reviewed

Journal of Thrombosis and Haemostasis   19 ( 6 )   1572 - 1584   2021.6

Heme activates platelets and exacerbates rhabdomyolysis-induced acute kidney injury via CLEC-2 and GPVI/FcRγ Reviewed

Heme activates platelets and exacerbates rhabdomyolysis-induced acute kidney injury via CLEC-2 and GPVI/FcRγ

Blood Advances   5 ( 7 )   2017 - 2026   2021.4

ヘミンはCLEC-2とGPVI/FcRγを介して血小板を活性化し、横紋筋融解による急性腎障害を悪化させる Reviewed

大竹 志門, 大石 沙織, 築地 長治, 大石 直輝, 佐々木 知幸, 白井 俊光, 高野 勝弘, 近藤 哲夫, 井上 克枝

日本透析医会雑誌   36 ( 1 )   141 - 145   2021.4( ISSN：0914-7136 )

Role of Platelet C‐Type Lectin‐Like Receptor 2 in Promoting Lung Metastasis in Osteosarcoma Reviewed

Jiro Ichikawa, Takashi Ando, Tomonori Kawasaki, Tomoyuki Sasaki, Toshiaki Shirai, Nagaharu Tsukiji, Yujiro Kimura, Kaoru Aoki, Keiko Hayakawa, Katsue Suzuki‐Inoue, Masao Saitoh, Hirotaka Haro

JOURNAL OF BONE AND MINERAL RESEARCH   35 ( 9 )   1738 - 1750   2020.9( ISSN：0884-0431 )

Platelet C-Type Lectin-Like Receptor 2 Reduces Cholestatic Liver Injury in Mice Reviewed

Suguru Maruyama,Hiroshi Kono, Shinji Furuya, Hiroki Shimizu, Ryo Saito, Katsutoshi Shoda, Hidenori Akaike, Naohiro Hosomura, Yoshihiko Kawaguchi, Hidetake Amemiya, Hiromichi Kawaida, Makoto Sudo, Shingo Inoue, Toshiaki Shirai, Katsue Suzuki-Inoue, Daisuke Ichikawa

AMERICAN JOURNAL OF PATHOLOGY   190 ( 9 )   1833 - 1842   2020.9( ISSN：0002-9440 )

Platelet CLEC-2 promotes hematogenous metastasis and thromboinflammation in tumor-bearing mice Reviewed

Toshiaki Shirai

　   31 ( 4 )   452 - 459   2020.6

Hepatic thrombopoietin gene silencing reduces platelet count and breast cancer progression in transgenic MMTV-PyMT mice. Reviewed Major achievement

Shirai T, Revenko AS, Tibbitts J, Ngo ATP, Mitrugno A, Healy LD, Johnson J, Tucker EI, Hinds MT, Coussens LM, McCarty OJT, Monia BP3, Gruber A

Blood Advances   3 ( 20 )   3080 - 3091   2019.10

Soluble CLEC-2 is generated independently of ADAM10 and is increased in plasma in acute coronary syndrome: comparison with soluble GPVI Reviewed

Osamu Inoue, Makoto Osada, Junya Nakamura, Fuminori Kazama, Toshiaki Shirai, Nagaharu Tsukiji, Tomoyuki Sasaki, Hiroshi Yokomichi, Tomotaka Dohi, Makoto Kaneko, Makoto Kurano, Mitsuru Oosawa, Shogo Tamura, Kaneo Satoh, Katsuhiro Takano, Katsumi Miyauchi, Hiroyuki Daida, Yutaka Yatomi, Yukio Ozaki, Katsue Suzuki-Inoue

INTERNATIONAL JOURNAL OF HEMATOLOGY   110 ( 3 )   285 - 294   2019.6( ISSN：0925-5710 )

Thrombotic microangiopathy as a cause of cardiovascular toxicity from the BCR-ABL1 tyrosine kinase inhibitor ponatinib. Reviewed

BLOOD   2019.1( ISSN：0006-4971 )

The role of coagulation and platelets in colon cancer-associated thrombosis. Reviewed

Mitrugno A, Tassi Yunga S, Sylman JL, Zilberman-Rudenko J, Shirai T, Hebert JF, Kayton R, Zhang Y, Nan X, Shatzel JJ, Esener S, Duvernay MT, Hamm HE, Gruber A, Williams CD, Takata Y, Armstrong R, Morgan TK, McCarty OJT.

AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY   2018.11( ISSN：0363-6143 )

Platelets play an essential role in murine lung development through Clec-2/podoplanin interaction Reviewed Major achievement

Nagaharu Tsukiji, Osamu Inoue, Mitsuru Morimoto, Norifumi Tatsumi, Hiroaki Nagatomo, Koji Ueta, Toshiaki　Shirai,　Tomoyuki　Sasaki,　Shimon　Otake　Shogo　Tamura, Toshiaki Tachibana, Masataka Okabe, Masanori　Hirashima,　Yukio　Ozaki,　Katsue　Suzuki-Inoue

BLOOD   132 ( 11 )   1167 - 1179   2018.9( ISSN：0006-4971 )

Cobalt hematoporphyrin inhibits CLEC-2-podoplanin interaction, tumor metastasis, and arterial/venous thrombosis in mice. Reviewed

Tsukiji N, Osada M, Sasaki T, Shirai T, Satoh K, Inoue O, Umetani N, Mochizuki C, Saito T, Kojima S, Shinmori H, Ozaki Y, Suzuki-Inoue K

Blood Advance   2 ( 17 )   2214 - 2225   2018.9

Functional characterization of recombinant snake verom rhodocytin: rhodocytin mutant blocks CLEC-2/podoplanin-dependent platelet aggregation and lung metastasis. Reviewed

Tomoyuki Sasaki, Toshiaki Shirai, Nagaharu Tsukiji, Shimon Otake, Shogo Tamura, Jiro Ichikawa, Makoto Osada, Kaneo Satoh, Yukio Ozaki, Katsue Suzuki-Inoue

JOURNAL OF THROMBOSIS AND HAEMOSTASIS   16 ( 5 )   960 - 972   2018.5( ISSN：1538-7933 )

Removal of the C-Terminal Domains of ADAMTS13 by Activated Coagulation Factor XI induces Platelet Adhesion on Endothelial Cells under Flow Conditions Reviewed

Kathleen S. Garland, Stephanie E. Reitsma, Toshiaki Shirai, Jevgenia Zilberman-Rudenko, Erik I. Tucker, David Gailani, Andras Gruber, Owen J.T.McCarty, Cristina Puy

Frontiers in Medicine   4   232   2017.12

ASK1 facilitates tumor metastasis through phosphorylation of an ADP receptor P2Y12 in platelets Reviewed

Miki Kamiyama, Toshiaki Shirai, Shogo Tamura, Katsue Suzuki-Inoue, Shogo Ehata, Kei Takahashi, Kohei Miyazono, Yoshihiro Hayakawa, Takehiro Sato, Kohsuke Takeda, Isao Naguro, Hidenori Ichijo

CELL DEATH AND DIFFERENTIATION   24 ( 12 )   2066 - 2076   2017.7( ISSN：1350-9047 )

The platelet-activating receptor C-type lectin receptor-2 plays an essential role in liver regeneration after partial hepatectomy in mice Reviewed

H Kono, H Fujii, K Suzuki-Inoue, O Inoue, S Furuya, K Hirayama, Y Akazawa, Y Nakata, C Sun, N Tsukiji, T Shirai, Y Ozaki

Journal of Thrombosis and Haemostasis   15   998 - 1008   2017.2

C-type lectin-like receptor 2 promotes hematogenous tumor metastasis and prothrombotic state in tumor-bearing mice Reviewed Major achievement

T Shirai, O Inoue, S Tamura, N Tsukiji, T Sasaki, H Endo, K Satoh, M Osada, H Sato-Uchida, H Fujii, Y Ozaki, K Suzuki-Inoue

Journal of Thrombosis and Haemostasis   15   513 - 525   2016.12

Regulation of biological responses by CLEC-2 Reviewed Major achievement

Clinical Immunology & Allergology   66 ( 2 )   122 - 128   2016.8

Podoplanin-positive periarteriolar stromal cells promote megakaryocyte growth and proplatelet formation in mice by CLEC-2 Reviewed Major achievement

Blood   127 ( 13 )   1701 - 1710   2016.3

Vascular Smooth Muscle Cells Stimulate Platelets and Facilitate Thrombus Formation through Platelet CLEC-2: Implications in Atherothrombosis. Reviewed Major achievement

PLoS One   10 ( 9 )   2015.9

Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells. Reviewed

The Journal of biological chemistry   287 ( 26 )   22241 - 52   2012.6

鉄によるCLEC-2依存性血小板活性化機構とその生理的意義

築地長治、横森良平、田草川一穂、大石沙織、白井俊光、佐々木知幸、髙野勝弘、井上克枝

第45回日本血栓止血学会学術集会  2023.6  一般社団法人　日本血栓止血学会

新型コロナウイルスによる血小板血栓形成メカニズムの解明

佐々木知幸、白井俊光、築地長治、大石沙織、横森良平、髙野勝弘、森石恆司、井上克枝

第45回日本血栓止血学会学術集会  2023.6  日本血栓止血学会

血小板活性化受容体CLEC-2と癌関連血栓症の病態

第54回日本動脈硬化学会総会・学術集会  2022.7 

Cancer-associated fibroblasts promote venous thrombosis through C-type lectin-like receptor 2/podoplanin in 3LL lung cancer mouse model International conference

Toshiaki Shirai, Tomoyuki Sasaki, Nagaharu Tsukiji, Saori Ohishi, Ryohei Yokomori, Katsuhiro Takano, Katsue Suzuki-Inoue

ISTH2022  2022.7  ISTH

癌関連線維芽細胞はCLEC-2/PDPNを介して癌関連血栓症を増悪させる

白井俊光、佐々木知幸、築地長治、大石沙織、横森良平、高野勝弘、井上克枝

第44回日本血栓止血学会学術集会  2022.6  一般社団法人　日本血栓止血学会

硫酸化多糖カラギーナンはCLEC-2依存症に血小板を活性化する

横森良平、白井俊光、佐々木知幸、築地長治、大石沙織、高野勝弘、井上克枝

第44回日本血栓止血学会学術集会  2022.6  一般社団法人　日本血栓止血学会

CLEC-2 promotes IGF-1 secretion from PDPN+ stromal cells and positively regulates erythropoiesis

2021.9 

横紋筋融解症に併発する腎障害における血小板CLEC-2及びGPVIの役割

大石沙織、築地長治、大竹志門、大石直輝、佐々木知幸、白井俊光、吉河佑莉、髙野勝弘、新森英之、犬飼岳史、近藤哲夫、井上克枝

第43回日本血栓止血学会学術集会  2021.5  一般社団法人　日本血栓止血学会

Using microfluidic devices to study blood-endothelium interface

Toshiaki Shirai

2020.7 

Heme Activates Platelets and Exacerbates Rhabdomyolysis-Induced Acute Kidney Injury via CLEC-2 and GPVI/FcRγ International conference

S. Oishi, N. Tsukiji, S. Otake, N. Oishi, T. Sasaki, T. Shirai, Y. Yoshikawa, K. Takano, T. Kondo, K. Suzuki-Inoue

ISTH 2020 Congress  2020.7  International Society on Thrombosis and Haemostasis

C-type lectin-like receptor 2 promotes hematogenous tumor metastasis and prothrombotic state in tumor-bearing mice

Toshiaki Shirai

2020.7 

巨核球・血小板上C-type lectin-like receptor 2 (CLEC-2)の赤血球造血における役割

大竹志門、白井俊光、築地長治、佐々木知幸、田村彰吾、髙野勝弘、尾崎由基男、井上克枝

第42回日本血栓止血学会学術集会  2020.6  一般社団法人　日本血栓止血学会

横紋筋融解症に併発する腎障害における血小板CLEC-2およびGPVIの役割

大石沙織、築地長治、大竹志門、大石直輝、佐々木知幸、白井俊光、吉河佑莉、髙野勝弘、近藤哲夫、井上克枝

第42回日本血栓止血学会学術集会  2020.6  一般社団法人　日本血栓止血学会

血小板受容体CLEC-2による関節リウマチの病態形成への関与

佐々木知幸、白井俊光、築地長治、小山賢介、田村彰吾、大竹志門、長田誠、佐藤金夫、波呂浩孝、尾崎由基男、井上克枝

第42回日本血栓止血学会学術集会  2020.6  一般社団法人　日本血栓止血学会

Platelet C-type Lectin-Like Receptor 2 (CLEC-2) is Required for Optimal Regulation of Erythropoiesis International conference

S. Otake, T. Shirai, N. Tsukiji, T. Sasaki, K. Satoh, S. Tamura, K. Takano, Y. Ozaki, K. Suzuki-Inoue

ISTH2019  2019.7  International Society on Thrombosis and Haemostasis (ISTH)

Hepatic Thrombopoietin Gene Silencing Induces Platelet Count Reduction within the Hemostatic Range and Reduces Breast Cancer Progression in MMTV-PyMT Mice. International conference

T. Shirai, A. Revenko, J. Tibbits, L. Coussens, O. McCarty, B. Monia, A. Gruber

ISTH2019  2019.7  International Society on Thrombosis and Haemostasis (ISTH)

Hepatic thrombopoietin gene silencing reduces cancer progression in the mammary glands of mice

Toshiaki Shirai, Alexey Revenko, Justin Tibbits, Lisa M Coussens, Owen JT McCarty, Brett P Monia, Andras Gruber

2019.6 

血小板・巨核球上のC型レクチン様受容体（CLEC-2）が、赤血球造血に及ぼす役割

大竹志門、白井俊光、築地長治、佐々木知幸、田村彰吾、尾崎由基男、井上克枝

第41回日本血栓止血学会学術集会  2019.6  一般社団法人　日本血栓止血学会

Antisense Oligonucleotide Targeting of Thrombopoietin Synthesis Reduces Platelet Count within the Hemostatic Range and Slows Progression of De Novo Mammary Carcinogenesis in the Mmtv-Pymt Mouse. International conference

ASH annual meeting 2018  2018.12  American Society of Hematology

Essential role of platelet-expressed C-type lectin-like receptor 2 (CLEC-2) in erythropoiesis

Shimon Otake, Toshiaki Shirai, Nagaharu Tsukiji, Tomoyuki Sasaki, Kaneo Satoh, Shogo Tamura, Yukio Ozaki, Katsue Suzuki-Inoue

2018.10 

GPVI依存性血小板凝集を惹起する蛇毒コンバルキシンの遺伝子組換え体の作製と機能解析

佐々木知幸、白井俊光、築地長治、大竹志門、田村彰吾、長田誠、佐藤金夫、尾崎由基男、井上克枝

第91回日本生化学会大会  2018.9  公益社団法人　日本生化学会

Effect of partial platelet count reduction using antisense MGDF oligonucleotide (TPO ASO) treatment on the breast cancer progression in MMTV-PyMT mice International conference

Gordon Research Conference-Hemostasis  2018.7 

血小板・巨核球上のC型レクチン様受容体（CLEC-2）が、赤芽球の分化、成熟に及ぼす役割

大竹志門、白井俊光、築地長治、佐々木知幸、田村彰吾、佐藤金夫、尾崎由基男、井上克枝

第40回日本血栓止血学会学術集会  2018.6  一般社団法人　日本血栓止血学会

血小板受容体GPVIアゴニスト蛇毒コンバルキシンの遺伝組換体の作製とその機能解析

佐々木知幸、白井俊光、築地長治、大竹志門、田村彰吾、長田誠、佐藤金夫、尾崎由基男、井上克枝

第40回日本血栓止血学会学術集会  2018.6  一般社団法人　日本血栓止血学会

脳梗塞の増悪因子であるアクロレインの抗血小板作用

梅谷徳彦、白井俊光、築地長治、佐々木知幸、田村彰吾、佐藤金夫、大竹志門、高野勝弘、横道洋司、尾崎由基男、井上克枝

第40回日本血栓止血学会学術集会  2018.6  一般社団法人　日本血栓止血学会

Platelets as a novel key player in organogenesis: Clec-2/podoplanin interaction regulates fetal lung development

Nagaharu Tsukiji, Osamu Inoue, Mitsuru Morimoto, Norifumi Tatsumi, Hiroaki Nagatomo, Koji Ueta, Toshiaki Shirai, Tomoyuki Sasaki, Shimon Otake, Shogo Tamura, Toshiaki Tachibana, Masataka Okabe, Masanori Hirashima, Yukio Ozaki, Katsue Suzuki-Inoue

The 40th Congress of the Japanese Society on Thrombosis and Hemostasis  2018.6 

Platelet Activation by CLEC-2-Podoplanin Interaction is Essential for Lung Development International conference

N.Tsukiji, O.Inoue, T.Shirai, S.Tamura, S.Otake, T.Sasaki, K.Satoh, Y.Ozaki, K.Suzuki-Inoue

XXVI ISTH Congress  2017.7  The International Society on Thrombosis and Hemostasis (ISTH)

An Inhibitory Mutant of Snake Venom Rhodocytin Blocks CLEC-2/Podoplanin Interaction-dependent Platelet Aggregation and Experimental Lung Metastasis International conference

T.Sasaki, T.Shirai, N.Tsukiji, S.Otake, S.Tamura, M.Osada, K.Satoh, Y.Ozaki, K.Suzuki-Inoue

XXVI ISTH Congress  2017.7  The International Society on Thrombosis and Hemostasis (ISTH)

A Small Molecule Ligand for CLEC-2 Blocking Podoplanin Binding Inhibits Experimental Tumor Metastasis and Thrombus Formation in Mice International conference

K.Suzuki-Inoue, H.Shinmori, M.Osada, T.Shirai, C.Mochizuki, S.Kojima, T.Saito, T.Sasaki, O.Inoue, Y.Ozaki

XXVI ISTH Congress  2017.7  The International Society on Thrombosis and Hemostasis (ISTH)

Regulation of Platelet String Formation on Endothelial Cells in the Presence of Coagulation under Shear International conference

T.Shirai, J.Zilberman-Rudenko, C.Puy, K.S.Garland, D.E.Sallee, S.E.Reitsma, A.Gruber, O.J.T.McCarty

XXVI ISTH Congress  2017.7  The International Society on Thrombosis and Hemostasis (ISTH)

C-Type Lectin-like Receptor 2 Promotes Hematogenous Tumor Metastasis and Prothrombotic State in Tumor-bearing Mice International conference

T.Shirai, O.Inoue, S.Tamura, N.Tsukiji, T.Sasaki, H.Endo, K.Satoh, M.Osada, H.Sato-Uchida, H.Fujii, Y.Ozaki, K.Suzuki-Inoue

XXVI ISTH Congress  2017.7  The International Society on Thrombosis and Hemostasis (ISTH)

血小板によるCLEC-2-Podoplaninシグナルを介した新たな肺胞形成メカニズム

築地　長治、井上　修、辰巳　徳史、岡部　正隆、森本　充、植田　康司、平島　正則、佐々木　知幸、白井　俊光、田村　彰吾、大竹　志門、佐藤　金夫、尾崎　由基男、井上　克枝

第39回日本血栓止血学会学術集会  2017.6  一般社団法人　日本血栓止血学会

関節リウマチにおける血小板受容体CLEC-2の役割 Invited

佐々木　知幸、白井　俊光、築地　長治、小山　賢介、田村　彰吾、大竹　志門、長田　誠、佐藤　金夫、波呂　浩孝、尾崎　由基男、井上　克枝

第39回日本血栓止血学会学術集会  2017.6  一般社団法人　日本血栓止血学会

血小板CLEC-2は肺内皮細胞分化を介して呼吸機能獲得に寄与する Major achievement

築地　長治,井上　修,田村　彰吾,白井　俊光,佐々木　知幸,佐藤　金夫,尾崎　由基男,井上　克枝

第38回日本血栓止血学会学術集会  2016.6 

遺伝子組換えロドサイチンを用いたCLEC-2依存性血小板凝集の制御解析 Major achievement

佐々木　知幸,白井　俊光,築地　長治,田村　彰吾,長田　誠,佐藤　金夫,尾崎　由基男,井上　克枝

第38回日本血栓止血学会学術集会  2016.6 

ポドプラニン結合阻害能を持つCLEC-2結合分子化合物リガンド Major achievement

井上　克枝,新森　英之,長田　誠,白井　俊光,望月　ちひろ,小嶋　聡一,斎藤　臣雄,佐々木　知幸,井上　修,尾崎　由基男

第38回日本血栓止血学会学術集会  2016.6 

A Novel Function of CLEC-2 in Megakaryopoiesis: CLEC-2/PDPN Microenvironment facilitates Expansion and Maturation of Megakaryocytes Major achievement

XXV Congress of the International Society of Thrombosis and Haemostasis  2015.6 

CLEC-2 Facilitates Hematogenous Tumor Metastasis and in vitro Tumor Growth but not in vivo Tumor Growth Major achievement

XXV Congress of the International Society of Thrombosis and Haemostasis  2015.6 

CONSTRUCTION OF EXXPRESSION SYSTEM FOR BIOLOGICALLY FUNCTIONAL RECOMBINANT RHODOCYTIN Major achievement

XXV Congress of the International Society of Thrombosis and Haemostasis  2015.6 

CLEC-2/PDPN axis を介した巨核球造血微小環境 Major achievement

田村　彰吾,井上　克枝,築地　長治,白井　俊光,佐々木　知幸,佐藤　金夫,尾崎　由基男

第16回日本検査血液学会  2015.6 

血小板活性化受容体CLEC-2結合蛇毒ロドサイチンの機能を保持したリコンビナント体発現系の構築 Major achievement

佐々木　知幸,田村　彰吾,白井　俊光,築地　長治,佐藤　金夫,井上　克枝,尾崎　由基男

第37回日本血栓止血学会学術集会  2015.5 

血小板活性化受容体CLEC-2のポドプラニン発現腫瘍の増殖における役割 Major achievement

白井　俊光,井上　修,平山　和義,遠藤　弘史,佐藤　一美,藤井　秀樹,井上　克枝,尾崎　由基男

第37回日本血栓止血学会学術集会  2015.5 

血小板は肺発生に必須である:血小板CLEC-2の機能解析 Major achievement

築地　長治,井上　修,田村　彰吾,白井　俊光,佐々木　知幸,佐藤　金夫,井上　克枝,尾崎　由基男

第37回日本血栓止血学会学術集会  2015.5 

CLEC-2/PDPN axisによる骨髄内微小環境の可能性:CLEC-2は巨核球造血を促進させる Major achievement

田村　彰吾,井上　克枝,白井　俊光,築地　長治,佐々木　知幸,佐藤　金夫,尾崎　由基男

第37回日本血栓止血学会学術集会  2015.5 

毒性の高いアルデヒドであるアクロレインの血小板に対する作用 Major achievement

梅谷　徳彦,白井　俊光,山田　舜介,田村　彰吾,築地　長治,佐々木　知幸,井上　克枝,尾崎　由基男

第37回日本血栓止血学会学術集会  2015.5 

A platelet activation receptor, C-type lectin-like receptor 2 (CLEC-2): from discovery to recent advances Major achievement

The 37th Congress of the Japanese Society on Thrombosis and Hemostasis  2015.5 

A role of CLEC-2 in tumor growth and metastasis Major achievement

XXIV Congress of the international society on thrombosis and haemostasis  2013.6 

血小板活性化受容体CLEC-2の癌の血行性転移、リンパ行性転移、増殖における役割 Major achievement

白井　俊光,井上　修,平山　和義,遠藤　弘史,佐藤　一美,藤井　秀樹,井上　克枝,尾崎　由基男

第35回日本血栓止血学会  2013.5