Updated on 2026/04/02

写真a

 
Shinohara Yoshiaki
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Medicine Basic Science for Clinical Medicine (Anatomy and System Biology) Professor
Title
Professor

Research History

  • Faculty of Medicine, University of Yamanashi   Department of Anatomy and Systems Biology, Faculty of Medicine   Professor

    2023.6

  • 山梨大学医学部   解剖学講座細胞生物学教室   教授

    2022.4 - 2023.5

  • 自治医科大学医学部   解剖学講座組織学部門   学内教授

    2019.4 - 2022.3

  • Graduate School of Medicine, Nagoya City University   Department of Integrative Anatomy   Senior Lecturer

    2017.10 - 2019.3

  • Graduate School of Medicine, Nagoya City University   Department of Integrative Anatomy   Assistant Professor

    2017.4 - 2017.9

  • RIKEN

    2009

  • RIKEN

    2008

  • RIKEN

    2007

  • RIKEN

    2006 - 2007

  • National Institute for Physiological Sciences

    2006

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Education

  • Kyoto University

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    Country： Japan

Research Areas

  • Life Science / Clinical pharmacy

  • Life Science / Physiology

  • Life Science / Anatomy

  • Life Science / Basic brain sciences

  • Life Science / Neuroscience-general

  • Life Science / Neuroscience-general

  • Life Science / Anatomy and histopathology of nervous system

  • Life Science / Physiology

  • Life Science / Clinical pharmacy

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Research Interests

  • Left-right asymmetry of the brain

  • 記憶

  • 脳の左右差

  • 海馬

  • マウス

  • シナプス

  • グルタミン酸受容体

Research Projects

  • 健康長寿を実現するための脳刺激実験を行う目的で、高齢ブタを用いた脳機能評価プラットフォームを創生する

    2023.4 - 2025.3

    山梨大学  大学発ベンチャー創成プロジェクト 

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    Authorship：Principal investigator 

  • Ultra-fast observation of cortico-hippocampal functional dynamics

    Grant number：20H03295  2020.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

  • Experience-induced hippocampal asymmetry in rodents

    Grant number：17H02221  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Shinohar Yoshiaki

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    We integrate our results of this research propose with KAKENHI 20H03295, which aims to investigate the cortico-hippocampal interactions modulated by postnatal stimuli. The reasons are as follows. 1) The comparisons between cortico-hippocampal and bilateral hippocampal interactions have greater scientific significance 2) The interactions between the cortex and hippocampus could cause left-right asymmetry of hippocampal functions. So, we submitted our results of cortico-hippocampal integration in bioRxiv, and now trying to publish the results. Then, we are seeking to publish our result of bilateral hippocampal asymmetry.

  • Modulatory effects of postnatal experience on rat hippocampal local field potentials

    Grant number：26282222  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Institute of Physical and Chemical Research  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Shinohara Yoshiaki

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    Postnatal environmental experience has significant effects on brain development. We reported that rat reared in enriched environment after weaning exbibits greater magnitudes and increased synchronized activities of hippocampal gamma oscillations than rats reared in isolated conditions. We found in longer periods, gamma oscillations and synchrony mature also in isolated rats, but much slower than those reared in enriched environment. Moreover, we found that astrocytic spontaneous calcium oscillations have modulatory effects on hippocampal local field potentials in environment-dependent manner.

  • In vivo study of left-right differences of the rodent hippocampus

    Grant number：23590273  2011 - 2013

    Japan Society for the Promotion of Science  The Institute of Physical and Chemical Research  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    SHINOHARA Yoshiaki

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    Though left-right asymmetry of the brain is well known among the general population, the physiological meanings remain unclear. To investigate how animal experience influence on brain asymmetry, we recorded local field potentials of rat hippocampi bilaterally from rat reared in enriched environment (ENR) and those reared in isolated condition (ISO). We found that the power of gamma oscillations tends to be greater in rats reared in ENR than those reared in an ISO. This experience-dependent gamma enhancement is consistently larger in the right hippocampus across subjects, coinciding with a lateralized increase of synaptic density in the right hippocampus. Moreover, interhemispheric coherence in ENR group is significantly elevated at the gamma frequency. These results suggest that ENR rearing sculpts the functional left-right asymmetry of hippocampal circuits by reorganization of synapses.

  • Left-right asymmetry of mouse hippocampal functions

    Grant number：21700440  2009 - 2010

    Japan Society for the Promotion of Science  The Institute of Physical and Chemical Research  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    SHINOHARA Yoshiaki

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    We reported better performance of right hippocampus than left in spatial memory task in mice. Next, we found that ipsilateral/ contralateral ratios of CA3-CA1 axonal innervations are dependent on the locations (i.e. layer in CA1) of the synapse (submitted). We are now analyzing left-right differences of hippocampal electrophysiological activities in vivo.

  • 哺乳類脳機能の左右差の意義解明のために、左右半球を連絡する原因分子を同定する

    Grant number：21650081  2009 - 2010

    日本学術振興会  科学研究費助成事業  挑戦的萌芽研究

    平瀬 肇, 篠原 良章, 眞鍋 理一郎

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    脳機能の左右差はヒトではよく知られているが、その分子的基盤は、ほとんど分かっていない。また、マウスを用いた左右差の分子基盤を探る実験でも、神経伝達物質受容体の定量等のボトム・アップ的アプローチによるものが殆どである。さらに、体軸を形成する遺伝子のスクリーニングは数多いが、それらはすべて動物の初期発生期に誘導される分子に限られており、成熟した個体に対する徹底した遺伝子の網羅的スクリーニング皆無であった。
    哺乳類左右脳の遺伝子発現の違いを解明するために、平成21年度に収集した成獣ラット海馬CA3領域サンプルを次世代DNAシークエンサーを利用して遺伝子解析を行った。まず、収集された左右の海馬CA3サンプルより、short RNAライブラリを調製した。ライブラリ調整には理化学研究所オミックス基盤領域(鶴見)で開発されたLNAを用いてアダプターダイマーを除去する最新式のプロトコルを採用した。その結果、1サンプルあたり、約1000万タグ、左右合計6サンプルのshort RNAのシークエンシングに成功した。シークエンスされたshort RNAの中、約9割がmiRBase15に登録されているmicro RNAであった。micmoRNAの発現で際立った左右差が認められるものは殆ど無かった。また残る1割においては、バイオインフォマティック的手法により新規microRNA解析を行ったところ十数種類の新規microRNA候補遺伝子が見付かった。新規microRNA候補遺伝子の中から発現(タグ数)が高い2つの遺伝子の発現をin situ hybridization法を用いて確認した。新規microRNA候補遺伝子でも左右発現差が顕著な物は皆無であった。

  • Physiological significance of left-right asymmetry of hippocampal synapse

    Grant number：19300114  2007 - 2008

    Japan Society for the Promotion of Science  National Institute for Physiological Sciences  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    SHIGEMOTO Ryuichi, SHINOHARA Yoshiaki

  • 海馬に左右非対称に分布する受容体のシナプスレベルでの定量的解析

    Grant number：18700344  2006 - 2007

    日本学術振興会  科学研究費助成事業  若手研究(B)

    篠原 良章

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    代表者は海馬のCA1錐体細胞のapical dendriteで、右と左の海馬で同側のCA3領域から受ける投射を比較した場合、左側のシナプスにはNR2Bサブユニットが、右側のシナプスにはGluR1サブユニットが、それぞれ逆側と比較して1.5倍多いことを見いだした。(但し、両側CA3からの投射を合わせるとその差は検出できない)当該研究の業績については現在投稿中であり、脳の左右差については心理学的な研究はたくさんあるが脳の分子から差を見いだそうという研究はほとんどないため、代表者の研究は分子と高次機能の差を埋める研究成果になると考えている。
    さらに、このNR2BとGluR1の量の相反関係は、左右の軸を比較したときにだけ見いだされるわけではなく、1個1個のシナプスでも、GluR1が多いシナプスではNR2Bが少なく、その逆も成り立つと考えられる。この仮説を検証するためにSDS freeze fracture replica labeling (SDS-FRL)法を用い、1つのシナプスからレプリカを2つ作成してGluR1とNR2Bの抗体で免疫染色を行った。すると、予想したように、単一シナプスでもGluR1とNR2Bに優位な逆相関が見られた。一方、他の主要なグルタミン酸受容体のNR2AサブユニットとNR2Bサブユニットでは相関はなく、NR2Aの左右の分布差も見られなかった。脳のグルタミン酸受容体同士の量的相関を示した研究としても、当研究は初のものであると考えている。

  • 代謝型グルタミン酸受容体の局在とその機能的意義の解析

    Grant number：02J03976  2002 - 2004

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    篠原 良章

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    代謝型グルタミン酸受容体のうち、プレシナプスの側にあるmGluR7は海馬では錐体細胞で発現しているが、この細胞の伸ばす軸索終末のポストシナプス側の細胞の種類によって、プレシナプスの末端にmGluR7が集積したり、しなかったりする。錐体細胞の同じ軸索が錐体細胞とソマトスタチン陽性の介在ニューロンとの両方にシナプスを作っている場合、錐体細胞側のシナプス終末にはmGluR7は集まってこないが、介在ニューロン側にはmGluR7は集まってくる。つまり、シナプスのポスト側からプレ側になんらかの情報がもたらされ、それでmGluR7を運ぶ機構があると考えられる。そこで、mGluR7をGFPと融合した分子を作成し、錐体細胞のみにmGluR7を発現するトランスジェニックマウスを作ることでこの分子の局在を観察したが、標識分子が過剰発現してしまい、錐体細胞の広い範囲にこの分子が広がってしまった。
    そこで、代謝型グルタミン酸受容体の海馬での左右の分布に焦点を当てることにした。海馬のグルタミン酸受容体のうち、イオン透過型の分子であるNMDA受容体は、海馬の左右で局在が異なることが代表者たちによって昨年度示された。更に、この左右の局在の差は、NMDA受容体のサブユニットによっても異なり、NR2Bサブユニットでは左右の分布に差があるが、他のNR2AサブユニットやNR1サブユニットでは差がないことが判明した。そこで、海馬の存在する代謝型グルタミン酸受容体でもこのサブユニットごとの局在差がないかどうか検討している。

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Papers

  • Organic cation transporter-3 mediates exogenous histamine uptake by primary mast cells. Reviewed

    Thuy-An Nguyen, Yuki Nakamura, Kentaro Yoshimura, Hiroaki Nagatomo, Keishi Narita, Nguyen Quoc Vuong Tran, Kayoko Ishimaru, Yoshiaki Kobayashi, Nobuhiro Nakano, Satoshi Tanaka, Yoshiaki Shinohara, Atsuhito Nakao

    The journal of allergy and clinical immunology. Global   4 ( 3 )   100499 - 100499   2025.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    BACKGROUND: Mast cells exhibit uptake of histamine from the extracellular microenvironment, which can affect the extent of histamine release following their activation. How mast cells take up exogenous histamine, however, is not fully understood. Organic cation transporter-3 (OCT3), which is encoded by Slc22a3, is a bidirectional plasma membrane transporter for small cation molecules (including histamine), which is expressed in many types of cells, including mast cells. However, its precise roles in mast cells remain uncertain. OBJECTIVE: This study examined whether OCT3 plays a role in histamine uptake in mast cells. METHODS: Bone marrow-derived mast cells (BMMCs) obtained from OCT3-deficient (Slc22a3-knockout [Slc22a3-KO]) or wild-type (WT) mice were analyzed by microscopic examination, quantitative PCR, and flow cytometry to assess morphology and differentiation. Intracellular monoamine levels were evaluated by using HPLC and histamine enzyme immunoassay. The FcεRI-mediated activation of BMMCs was evaluated by β-hexosaminidase assay, ELISA, Western blot, and calcium flux assay. Passive cutaneous anaphylaxis reaction was used for in vivo analysis. RESULTS: The WT and Slc22a3-KO mouse BMMCs were comparable in morphology and differentiation. The Slc22a3-KO mouse BMMCs exhibited low intracellular histamine levels associated with low uptake of extracellular histamine and showed a reduction of histamine release following FcεRI activation when compared with WT BMMCs. Compared with WT mice Slc22a3-KO mice exhibited a reduction in passive cutaneous anaphylaxis reaction. CONCLUSION: These findings suggest that OCT3 mediates exogenous histamine uptake by primary mast cells and that deficiency of OCT3 reduces intracellular histamine levels and downregulates IgE-mediated histamine release in mast cells.

  • Brain state-dependent neocortico-hippocampal network dynamics are modulated by postnatal stimuli. Reviewed Major achievement

    Yoshiaki Shinohara, Shinnosuke Koketsu, Nobuhiko Ohno, Hajime Hirase, Takatoshi Ueki

    The Journal of neuroscience : the official journal of the Society for Neuroscience   2025.1

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Neurons in the cerebral cortex and hippocampus discharge synchronously in brain state-dependent manner to transfer information. Published studies have highlighted the temporal coordination of neuronal activities between the hippocampus and a neocortical area, however, how the spatial extent of neocortical activity relates to hippocampal activity remains partially unknown. We imaged mesoscopic neocortical activity while recording hippocampal local field potentials in anesthetized and unanesthetized GCaMP-expressing transgenic mice. We found that neocortical activity elevates around hippocampal sharp wave ripples (SWR). SWR-associated neocortical activities occurred predominantly in vision-related regions including visual, retrosplenial and frontal cortex. While pre-SWR neocortical activities were frequently observed in awake and natural sleeping states, post-SWR neocortical activity decreased significantly in the latter. Urethane anesthetized mice also exhibited SWR-correlated calcium elevation, but in longer time scale than observed in natural sleeping mice. During hippocampal theta oscillation states, phase-locked oscillations of calcium activity were observed throughout the entire neocortical areas. In addition, possible environmental effects on neocortico-hippocampal dynamics were assessed in this study by comparing mice reared in ISO (isolated condition) and ENR (enriched environment). In both SWR and theta oscillations, mice reared in ISO exhibited clearer brain state-dependent dynamics than those reared in ENR. Our data demonstrate that the neocortex and hippocampus exhibit heterogeneous activity patterns that characterize brain states, and postnatal experience plays a significant role in modulating these patterns.Significant Statement The hippocampus is a center for memory formation. However, the memory formed in the hippocampus is not stored forever, but gradually transferred into the cerebral cortex synchronized activities between the neocortex and hippocampus has been hypothesized (for hippocampus-independent memory see (Sutherland and Rudy, 1989)). However, spatio-temporal dynamics between hippocampus and whole neocortical areas remains partially unexplored. We measured cortical calcium activities with hippocampal electroencephalogram (EEG) simultaneously and found that the activities of widespread neocortical areas are temporally associated with hippocampal EEG. The neocortico-hippocampal dynamics is primarily regulated by animal awake/sleep state. Even if similar EEG patters were observed, temporal dynamics between the neocortex and hippocampus exhibit distinct patterns between awake and sleep period. In addition, animals' postnatal experience modulates the dynamics.

  • Housing environment bilaterally alters transcriptomic profile in the rat hippocampal CA1 region. Reviewed Major achievement

    Azusa Kubota, Kentaro Kojima, Shinnosuke Koketsu, Takayuki Kannon, Takehiro Sato, Kazuyoshi Hosomichi, Yoshiaki Shinohara, Atsushi Tajima

    PloS one   20 ( 12 )   e0338190   2025

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Brain asymmetry is a fundamental feature of neural organization. However, the molecular basis of hippocampal lateralization in response to environmental stimuli remains poorly understood. Here, we examined the transcriptomic profiles of the left and right hippocampal CA1 regions in rats reared under isolated or enriched housing conditions to elucidate hemisphere-specific responses and shared molecular adaptations. RNA-sequencing analysis revealed lateralized differences in the number and identity of differentially expressed genes, accompanied by distinct biological themes, as indicated by overrepresentation and gene set enrichment analysis. The left CA1 region was prominently engaged in pathways related to synaptic organization and mitochondrial function, whereas the right CA1 region exhibited enrichment in transcriptional regulation and RNA metabolic processes. Despite these asymmetries, co-expression and protein-protein interaction network analyses revealed shared molecular architectures. Immediate early genes formed consistent central hubs across both hemispheres, and a common Mecp2-Grin2b-Cdkl5-Tet3 protein interaction cluster was identified as a potential integrative regulatory module. Additional enrichment analysis of differentially expressed genes shared between hemispheres further highlighted conserved responses, particularly in synaptic plasticity and cell-cell communication. Together, these findings demonstrate that the left and right CA1 regions employ distinct yet partially convergent transcriptional programs to adapt to environmental stimuli. This coordinated molecular asymmetry provides novel insights into hippocampal lateralization and its role in experience-dependent brain plasticity.

    DOI： 10.1371/journal.pone.0338190

    PubMed

  • Axonal Selectivity of Myelination by Single Oligodendrocytes Established During Development in Mouse Cerebellar White Matter. Reviewed

    Batpurev Battulga, Yasuyuki Osanai, Reiji Yamazaki, Yoshiaki Shinohara, Nobuhiko Ohno

    Glia   2024.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Myelin formation by oligodendrocytes regulates the conduction velocity and functional integrity of neuronal axons. While individual oligodendrocytes form myelin sheaths around multiple axons and control the functions of neural circuits where the axons are involved, it remains unclear if oligodendrocytes selectively form myelin sheaths around specific subtypes of axons. Using the combination of rabies virus-mediated single oligodendrocyte labeling and immunostaining with tissue clearing, we revealed that approximately half of the oligodendrocytes preferentially myelinate axons originating from Purkinje cells in the white matter of adult mouse cerebella. The preference for Purkinje cell axons was more pronounced during development when the process of myelination within cerebellar white matter was initiated; over 90% of oligodendrocytes preferentially myelinated Purkinje cell axons. Preferential myelination of Purkinje cell axons was further confirmed by immuno-electron microscopy and transgenic mice that label early-born oligodendrocytes. Transgenic mice that label oligodendrocytes differentiated at the early development showed that early-born oligodendrocytes preferentially myelinate Purkinje cell axons in the matured cerebellar white matter. In contrast, transgenic mice that label oligodendrocytes differentiated after the peak of cerebellar myelination showed that the later-differentiated oligodendrocytes dominantly myelinated non-Purkinje cell axons. These results demonstrate that a significant proportion of oligodendrocytes preferentially myelinate functionally distinct axons in the cerebellar white matter, and the axonal preference of myelination by individual oligodendrocytes is established depending on the timing of their differentiation during development. Our data provide the evidence that there is a critical time window of myelination that a specific subtype of axons are dominantly myelinated by the oligodendrocytes.

    DOI： 10.1002/glia.24660

    PubMed

  • The defects of the hippocampal ripples and theta rhythm in depression, and the effects of physical exercise on their amelioration Major achievement

    Koketsu S, Matsubara K, Ueki Y, Shinohara Y, Inoue K, Murakami S, Ueki T

    Heliyon   10 ( 1 )   2024.1

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    Language：English   Publishing type：Research paper (scientific journal)  

  • 乏突起膠細胞の調査 マウス小脳におけるニューロン相互作用(Investigation of oligodendrocyte - neuron interaction in the mice cerebellum)

    バッツルガ・バトブレブ, 長内 康幸, 山崎 礼二, 矢田部 恵, 篠原 良章, 大野 伸彦

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集   64回   38 - 38   2023.10

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    Language：English   Publisher：日本組織細胞化学会  

  • Projections of hippocampal CA2 pyramidal neurons: Distinct innervation patterns of CA2 compared to CA3 in rodents. Invited Reviewed Major achievement

    Yoshiaki Shinohara, Keigo Kohara

    Hippocampus   33 ( 6 )   691 - 699   2023.6

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The hippocampus is a center for spatial and episodic memory formation in rodents. Understanding the composition of subregions and circuitry maps of the hippocampus is essential for elucidating the mechanism of memory formation and recall. For decades, the trisynaptic circuit (entorhinal cortex layer II-dentate gyrus - CA3-CA1) has been considered the neural network substrate responsible for learning and memory. Recently, CA2 has emerged as an important area in the hippocampal circuitry, with distinct functions from those of CA3. In this article, we review the historical definition of the hippocampal area CA2 and the differential projection patterns between CA2 and CA3 pyramidal neurons. We provide a concise and comprehensive map of CA2 outputs by comparing (1) ipsi versus contra projections, (2) septal versus temporal projections, and (3) lamellar structures of CA2 and CA3 pyramidal neurons.

    DOI： 10.1002/hipo.23519

    PubMed

  • Dark Rearing in the Visual Critical Period Causes Structural Changes in Myelinated Axons in the Adult Mouse Visual Pathway. Reviewed

    Yasuyuki Osanai, Batpurev Battulga, Reiji Yamazaki, Tom Kouki, Megumi Yatabe, Hiroaki Mizukami, Kenta Kobayashi, Yoshiaki Shinohara, Yumiko Yoshimura, Nobuhiko Ohno

    Neurochemical research   47 ( 9 )   2815 - 2825   2022.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    An appropriate sensory experience during the early developmental period is important for brain maturation. Dark rearing during the visual critical period delays the maturation of neuronal circuits in the visual cortex. Although the formation and structural plasticity of the myelin sheaths on retinal ganglion cell axons modulate the visual function, the effects of dark rearing during the visual critical period on the structure of the retinal ganglion cell axons and their myelin sheaths are still unclear. To address this question, mice were reared in a dark box during the visual critical period and then normally reared to adulthood. We found that myelin sheaths on the retinal ganglion cell axons of dark-reared mice were thicker than those of normally reared mice in both the optic chiasm and optic nerve. Furthermore, whole-mount immunostaining with fluorescent axonal labeling and tissue clearing revealed that the myelin internodal length in dark-reared mice was shorter than that in normally reared mice in both the optic chiasm and optic nerve. These findings demonstrate that dark rearing during the visual critical period affects the morphology of myelin sheaths, shortens and thickens myelin sheaths in the visual pathway, despite the mice being reared in normal light/dark conditions after the dark rearing.

    DOI： 10.1007/s11064-022-03689-8

    PubMed

  • 組織細胞化学を応用したボリューム電子顕微鏡イメージング

    大野 伸彦, 齊藤 百合花, 長内 康幸, 山崎 礼二, 篠原 良章

    組織細胞化学   2022   165 - 176   2022.7

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    Language：Japanese   Publisher：日本組織細胞化学会  

  • Heterogeneity and regulation of oligodendrocyte morphology. Reviewed

    Yasuyuki Osanai, Reiji Yamazaki, Yoshiaki Shinohara, Nobuhiko Ohno

    Frontiers in cell and developmental biology   10   1030486 - 1030486   2022

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    Language：English   Publishing type：Research paper (scientific journal)  

    Oligodendrocytes form multiple myelin sheaths in the central nervous system (CNS), which increase nerve conduction velocity and are necessary for basic and higher brain functions such as sensory function, motor control, and learning. Structures of the myelin sheath such as myelin internodal length and myelin thickness regulate nerve conduction. Various parts of the central nervous system exhibit different myelin structures and oligodendrocyte morphologies. Recent studies supported that oligodendrocytes are a heterogenous population of cells and myelin sheaths formed by some oligodendrocytes can be biased to particular groups of axons, and myelin structures are dynamically modulated in certain classes of neurons by specific experiences. Structures of oligodendrocyte/myelin are also affected in pathological conditions such as demyelinating and neuropsychiatric disorders. This review summarizes our understanding of heterogeneity and regulation of oligodendrocyte morphology concerning central nervous system regions, neuronal classes, experiences, diseases, and how oligodendrocytes are optimized to execute central nervous system functions.

    DOI： 10.3389/fcell.2022.1030486

    PubMed

  • Myelination in visual pathway is modulated by relative neuronal activity between eyes.

    長内康幸, バッツルガ バトプレブ, 山崎礼二, 山本真理子, 幸喜富, 矢田部恵, 小林憲太, 中村由香, 上野将紀, 水上浩明, 篠原良章, 吉村由美子, 吉村由美子, 大野伸彦, 大野伸彦

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   127th   2022

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    Language：Japanese  

    J-GLOBAL

  • 視覚伝導路における神経活動に依存した髄鞘形成機構の解明

    長内 康幸, バッツルガ・バツプレブ, 山崎 礼二, 山本 真理子, 幸喜 富, 矢田部 恵, 水上 浩明, 上野 将紀, 小林 憲太, 吉村 由美子, 篠原 良章, 大野 伸彦

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集   62回   68 - 68   2021.9

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    Language：Japanese   Publisher：日本組織細胞化学会  

  • Macroscopic detection of demyelinated lesions in mouse PNS with neutral red dye. Reviewed

    Reiji Yamazaki, Yasuyuki Osanai, Tom Kouki, Yoshiaki Shinohara, Jeffrey K Huang, Nobuhiko Ohno

    Scientific reports   11 ( 1 )   16906 - 16906   2021.8

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    Language：English   Publishing type：Research paper (scientific journal)  

    Lysophosphatidylcholine (LPC)-induced demyelination is a versatile animal model that is frequently used to identify and examine molecular pathways of demyelination and remyelination in the central (CNS) and peripheral nervous system (PNS). However, identification of focally demyelinated lesion had been difficult and usually required tissue fixation, sectioning and histological analysis. Recently, a method for labeling and identification of demyelinated lesions in the CNS by intraperitoneal injection of neutral red (NR) dye was developed. However, it remained unknown whether NR can be used to label demyelinated lesions in PNS. In this study, we generated LPC-induced demyelination in sciatic nerve of mice, and demonstrated that the demyelinated lesions at the site of LPC injection were readily detectable at 7 days postlesion (dpl) by macroscopic observation of NR labeling. Moreover, NR staining gradually decreased from 7 to 21 dpl over the course of remyelination. Electron microscopy analysis of NR-labeled sciatic nerves at 7 dpl confirmed demyelination and myelin debris in lesions. Furthermore, fluorescence microscopy showed NR co-labeling with activated macrophages and Schwann cells in the PNS lesions. Together, NR labeling is a straightforward method that allows the macroscopic detection of demyelinated lesions in sciatic nerves after LPC injection.

    DOI： 10.1038/s41598-021-96395-4

    PubMed

  • ボリューム電子顕微鏡イメージングにおける組織細胞化学の特徴

    大野 伸彦, 齊藤 百合花, 長内 康幸, 山崎 礼二, 篠原 良章

    組織細胞化学   2021   163 - 174   2021.8

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    Language：Japanese   Publisher：日本組織細胞化学会  

  • Adrenergic inhibition facilitates normalization of extracellular potassium after cortical spreading depolarization. Reviewed

    Hiromu Monai, Shinnosuke Koketsu, Yoshiaki Shinohara, Takatoshi Ueki, Peter Kusk, Natalie L Hauglund, Andrew J Samson, Maiken Nedergaard, Hajime Hirase

    Scientific reports   11 ( 1 )   8150 - 8150   2021.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    Cortical spreading depolarization (CSD) is a propagating wave of tissue depolarization characterized by a large increase of extracellular potassium concentration and prolonged subsequent electrical silencing of neurons. Waves of CSD arise spontaneously in various acute neurological settings, including migraine aura and ischemic stroke. Recently, we have reported that pan-inhibition of adrenergic receptors (AdRs) facilitates the normalization of extracellular potassium after acute photothrombotic stroke in mice. Here, we have extended that mechanistic study to ask whether AdR antagonists also modify the dynamics of KCl-induced CSD and post-CSD recovery in vivo. Spontaneous neural activity and KCl-induced CSD were visualized by cortex-wide transcranial Ca2+ imaging in G-CaMP7 transgenic mice. AdR antagonism decreased the recurrence of CSD waves and accelerated the post-CSD recovery of neural activity. Two-photon imaging revealed that astrocytes exhibited aberrant Ca2+ signaling after passage of the CSD wave. This astrocytic Ca2+ activity was diminished by the AdR antagonists. Furthermore, AdR pan-antagonism facilitated the normalization of the extracellular potassium level after CSD, which paralleled the recovery of neural activity. These observations add support to the proposal that neuroprotective effects of AdR pan-antagonism arise from accelerated normalization of extracellular K+ levels in the setting of acute brain injury.

    DOI： 10.1038/s41598-021-87609-w

    PubMed

  • Perivascular macrophages produce type I collagen around cerebral small vessels under prolonged hypertension in rats Reviewed

    Takeshi Inagaki, Ken Fujiwara, Yoshiaki Shinohara, Morio Azuma, Reiji Yamazaki, Kiyomi Mashima, Atsushi Sakamoto, Takashi Yashiro, Nobuhiko Ohno

    Histochemistry and Cell Biology   2021.1( ISSN：0948-6143  eISSN：1432-119X )

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    DOI： 10.1007/s00418-020-01948-9

    Other Link： http://link.springer.com/article/10.1007/s00418-020-01948-9/fulltext.html

  • Oligodendrocytes form normal myelin in the visual pathway when all axons are derived from deprived eyes

    OSANAI Yasuyuki, BATTULGA Batpurev, YAMAZAKI Reiji, YAMAMOTO Mariko, KOUKI Tom, YATABE Megumi, MIZUKAMI Hiroaki, UENO Masaki, KOBAYASHI Kenta, YOSHIMURA Yumiko, YOSHIMURA Yumiko, SHINOHARA Yoshiaki, OHNO Nobuhiko, OHNO Nobuhiko

    日本神経化学会大会抄録集(Web)   64th   2021

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    Language：Japanese  

    J-GLOBAL

  • 脳細動脈硬化における血管線維化と脳血管周囲マクロファージの関連

    稲垣健志, 山崎礼二, 長内康幸, 篠原良章, 坂本敦司, 大野伸彦

    日本臨床分子形態学会総会・学術集会講演プログラム・要旨集   53rd   2021

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    Language：Japanese  

    J-GLOBAL

  • 末梢神経系脱髄に対する新たな病変部位標識法の開発

    山崎 礼二, 長内 康幸, 幸喜 富, 篠原 良章, 大野 伸彦

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集   61回   55 - 55   2020.12

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    Language：Japanese   Publisher：日本組織細胞化学会  

  • 視覚臨界期における視覚欠如がオリゴデンドロサイトの形態に与える影響の解析

    長内 康幸, 山崎 礼二, 幸喜 富, 矢田部 恵, 山本 真理子, 吉村 由美子, 篠原 良章, 大野 伸彦

    日本組織細胞化学会総会・学術集会講演プログラム・予稿集   61回   47 - 47   2020.12

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    Language：Japanese   Publisher：日本組織細胞化学会  

  • Potential implication of SGK1-dependent activity change in BV-2 microglial cells. Reviewed

    Hayato Asai, Koichi Inoue, Eisuke Sakuma, Yoshiaki Shinohara, Takatoshi Ueki

    International journal of physiology, pathophysiology and pharmacology   10 ( 2 )   115 - 123   2018( ISSN：1944-8171 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    It has recently been established that microglial activation is involved in the pathophysiology of various neurological and psychiatric disorders such as amyotrophic lateral sclerosis and schizophrenia. The pathological molecular machineries underlying microglial activation and its accelerating molecules have been precisely described in the diseased central nervous system (CNS). However, to date, the details of physiological mechanism, which represses microglial activation, are still to be elucidated. Our latest report demonstrated that serum- and glucocorticoid-inducible kinases (SGK1 and SGK3) were expressed in multiple microglial cell lines, and their inhibitor enhanced the toxic effect of lipopolysaccharide on microglial production of inflammatory substances such as TNFα and iNOS. In the present report, we prepared SGK1-lacked microglial cell line (BV-2) and demonstrated that deficiency of SGK1 in microglia induced its toxic conversion, in which it took amoeboid morphology characteristic of reactive microglia, increased CD68 expression, quickened its proliferation, and showed higher susceptibility to ATP and subsequent cell death. Our data indicate that SGK1 plays pivotal roles in inhibiting its pathological activation, and suggest its potential function as a therapeutic target for the treatment of various disorders related to the inflammation in the CNS.

    PubMed

  • Rearing-environment-dependent hippocampal local field potential differences in wild-type and inositol trisphosphate receptor type 2 knockout mice Reviewed Major achievement

    Mika Tanaka, Xiaowen Wang, Katsuhiko Mikoshiba, Hajime Hirase, Yoshiaki Shinohara

    JOURNAL OF PHYSIOLOGY-LONDON   595 ( 20 )   6557 - 6568   2017.10( ISSN：0022-3751  eISSN：1469-7793 )

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    Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1113/JP274573

    Web of Science

  • Network mechanisms of hippocampal laterality, place coding, and goal-directed navigation. Invited Reviewed

    Takuma Kitanishi, Hiroshi T Ito, Yuichiro Hayashi, Yoshiaki Shinohara, Kenji Mizuseki, Takatoshi Hikida

    The journal of physiological sciences : JPS   67 ( 2 )   247 - 258   2017.3( ISSN：1880-6546 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s12576-016-0502-z

    Web of Science

    PubMed

  • Adaptive changes of extracellular amino acid concentrations in mouse dorsal striatum by 4-AP-induced cortical seizures. Reviewed

    Nagai T, Takata N, Shinohara Y, Hirase H

    Neuroscience   295   229 - 236   2015.6( ISSN：0306-4522 )

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  • TRANSFORMATION OF CORTICAL AND HIPPOCAMPAL NEURAL CIRCUIT BY ENVIRONMENTAL ENRICHMENT Major achievement

    H. Hirase, Y. Shinohara

    NEUROSCIENCE   280   282 - 298   2014.11( ISSN：0306-4522  eISSN：1873-7544 )

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    Language：English   Publishing type：(MISC) Book review and document introduction, etc.  

    DOI： 10.1016/j.neuroscience.2014.09.031

    Web of Science

  • Volume transmission signalling via astrocytes Reviewed

    Hajime Hirase, Youichi Iwai, Norio Takata, Yoshiaki Shinohara, Tsuneko Mishima

    PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES   369 ( 1654 )   2014.10( ISSN：0962-8436  eISSN：1471-2970 )

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    Language：English  

    DOI： 10.1098/rstb.2013.0604

    Web of Science

  • Experience enhances gamma oscillations and interhemispheric asymmetry in the hippocampus (vol 4, 1652, 2013) Reviewed Major achievement

    Yoshiaki Shinohara, Aki Hosoya, Hajime Hirase

    NATURE COMMUNICATIONS   5   2014.5( ISSN：2041-1723 )

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    Language：English  

    DOI： 10.1038/ncomms4693

    Web of Science

  • Imaging of Astrocytic Activity in Living Rodents. Reviewed

    Takata N, Shinohara Y, Ohkura M, Mishima T, Nakai J, Hirase H

    Neuromethods   85   191 - 207   2014

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    Language：English  

    DOI： 10.1007/978-1-62703-785-3_12

  • ULTRASTRUCTURAL INVESTIGATION OF LATERALIZED EXPERIENCE-DEPENDENT SYNAPTIC PLASTICITY IN RAT HIPPOCAMPAL CA1 STRATUM RADIATUM Major achievement

    Y. Shinohara, A. Hosoya, H. Hirase

    GLIA   61   S114 - S114   2013.7( ISSN：0894-1491 )

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    Language：English   Publishing type：(MISC) Summary of the papers read (international conference)  

    Web of Science

  • Experience enhances gamma oscillations and interhemispheric asymmetry in the hippocampus Reviewed Major achievement

    Yoshiaki Shinohara, Aki Hosoya, Hajime Hirase

    NATURE COMMUNICATIONS   4   2013.4( ISSN：2041-1723 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/ncomms2658

    Web of Science

  • Regulation of synaptic currents by mGluR2 at reciprocal synapses in the mouse accessory olfactory bulb Reviewed

    Mutsuo Taniguchi, Mineto Yokoi, Yoshiaki Shinohara, Fumino Okutani, Yoshihiro Murata, Shigetada Nakanishi, Hideto Kaba

    EUROPEAN JOURNAL OF NEUROSCIENCE   37 ( 3 )   351 - 358   2013.2( ISSN：0953-816X )

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/ejn.12059

    Web of Science

  • Quantification of postsynaptic density proteins: Glutamate receptor subunits and scaffolding proteins Invited Reviewed Major achievement

    Yoshiaki Shinohara

    HIPPOCAMPUS   22 ( 5 )   942 - 953   2012.5( ISSN：1050-9631 )

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    Authorship：Lead author,　Corresponding author   Language：English  

    DOI： 10.1002/hipo.20950

    Web of Science

  • Hippocampal CA3 and CA2 have distinct bilateral innervation patterns to CA1 in rodents Invited Reviewed Major achievement

    Yoshiaki Shinohara, Aki Hosoya, Kazuko Yahagi, Alex S. Ferecsko, Kunio Yaguchi, Attila Sik, Makoto Itakura, Masami Takahashi, Hajime Hirase

    EUROPEAN JOURNAL OF NEUROSCIENCE   35 ( 5 )   702 - 710   2012.3( ISSN：0953-816X )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/j.1460-9568.2012.07993.x

    Web of Science

  • Right-hemispheric dominance of spatial memory in split-brain mice Reviewed Major achievement

    Yoshiaki Shinohara, Aki Hosoya, Nobuyuki Yamasaki, Hassan Ahmed, Satoko Hattori, Megumi Eguchi, Shun Yamaguchi, Tsuyoshi Miyakawa, Hajime Hirase, Ryuichi Shigemoto

    HIPPOCAMPUS   22 ( 2 )   117 - 121   2012.2( ISSN：1050-9631 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1002/hipo.20886

    Web of Science

    PubMed

  • miRNA profiling of bilateral rat hippocampal CA3 by deep sequencing Reviewed Major achievement

    Yoshiaki Shinohara, Kazuko Yahagi, Mitsuoki Kawano, Hiromi Nishiyori, Chika Kawazu, Naoko Suzuki, Ri-ichiroh Manabe, Hajime Hirase

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   409 ( 2 )   293 - 298   2011.6( ISSN：0006-291X )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.bbrc.2011.05.004

    Web of Science

  • Enriched environment enhances left-right asymmetry of the hippocampus Major achievement

    Yoshiaki Shinohara, Aki Hosoya, Hajime Hirase

    NEUROSCIENCE RESEARCH   71   E176 - E176   2011( ISSN：0168-0102 )

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    Language：English   Publishing type：(MISC) Summary of the papers read (international conference)  

    DOI： 10.1016/j.neures.2011.07.762

    Web of Science

  • Size and receptor density of glutamatergic synapses: a viewpoint from left-right asymmetry of CA3-CA1 connections Reviewed Major achievement

    Yoshiaki Shinohara, Hajime Hirase

    FRONTIERS IN NEUROANATOMY   3   2009.7( ISSN：1662-5129 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.3389/neuro.05.010.2009

    Web of Science

  • Left-right asymmetry of the hippocampal synapses with differential subunit allocation of glutamate receptors Reviewed Major achievement

    Yoshiaki Shinohara, Hajime Hirase, Masahiko Watanabe, Makoto Itakura, Masami Takahashi, Ryuichi Shigemoto

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   105 ( 49 )   19498 - 19503   2008.12( ISSN：0027-8424 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.0807461105

    Web of Science

  • Neural-Activity-Dependent Release of S100B from Astrocytes Enhances Kainate-Induced Gamma Oscillations In Vivo Reviewed Major achievement

    Seiichi Sakatani, Akiko Seto-Ohshima, Yoshiaki Shinohara, Yasuhiko Yamamoto, Hiroshi Yamamoto, Shigeyoshi Itohara, Hajime Hirase

    JOURNAL OF NEUROSCIENCE   28 ( 43 )   10928 - 10936   2008.10( ISSN：0270-6474 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1523/JNEUROSCI.3693-08.2008

    Web of Science

  • Left-right asymmetry of mouse hippocampal pyramidal neurons Reviewed Major achievement

    Yoshiaki Shinohara, Ryuichi Shigemoto

    NEUROSCIENCE RESEARCH   58   S188 - S188   2007( ISSN：0168-0102 )

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  • Target-cell-specific left-right asymmetry of NMDA receptor content in Schaffer collateral synapses in is an element of 1/NR2A knock-out mice Reviewed Major achievement

    Y Wu, R Kawakami, Y Shinohara, M Fukaya, K Sakimura, M Mishina, M Watanabe, Ito, I, R Shigemoto

    JOURNAL OF NEUROSCIENCE   25 ( 40 )   9213 - 9226   2005.10( ISSN：0270-6474 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1523/JNEUROSCI.2134-05.2005

    Web of Science

    PubMed

  • Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC Reviewed

    M Uchino, N Sakai, K Kashiwagi, Y Shirai, Y Shinohara, K Hirose, M Iino, T Yamamura, N Saito

    JOURNAL OF BIOLOGICAL CHEMISTRY   279 ( 3 )   2254 - 2261   2004.1( ISSN：0021-9258 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M309894200

    Web of Science

    PubMed

  • Asymmetrical allocation of NMDA receptor epsilon 2 subunits in hippocampal circuitry Reviewed

    R Kawakami, Y Shinohara, Y Kato, H Sugiyama, R Shigemoto, Ito, I

    SCIENCE   300 ( 5621 )   990 - 994   2003.5( ISSN：0036-8075 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1082609

    Web of Science

    PubMed

  • Oscillatory translocation of gamma PKC by the activation of metabotropic glutamate receptor and its regulation by delta PKC in a subtype specific manner.

    M Uchino, N Sakai, Y Shirai, K Hirose, M Iino, Y Shinohara, S Nakanishi, N Saito

    JAPANESE JOURNAL OF PHARMACOLOGY   88   175P - 175P   2002( ISSN：0021-5198 )

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    Language：English   Publishing type：(MISC) Summary of the papers read (international conference)  

    Web of Science

  • Glutamate induces focal adhesion kinase tyrosine phosphorylation and actin rearrangement in heterologous mGluR1-expressing CHO cells via calcium/calmodulin signaling Reviewed Major achievement

    Yoshiaki Shinohara, Yoshiaki Nakajima, Shigetada Nakanishi

    Journal of Neurochemistry   78 ( 2 )   365 - 373   2001( ISSN：0022-3042 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1046/j.1471-4159.2001.00415.x

    Scopus

    PubMed

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Presentations

  • マイクロミニブタ脳の全脳マッピング解析 Major achievement

    篠原 良章

    第11回日本先進医工学ブタ研究会  2025.11 

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    Event date： 2025.11

    Language：Japanese   Presentation type：Symposium workshop panel(public)  

  • Brain state-dependent neocortico-hippocampal network dynamics are modulated by postnatal stimuli

    Yoshiaki Shinohara

    The 48th Annual Meeting of the Japan Neuroscience Society  2025.7 

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    Event date： 2025.7

    Language：English   Presentation type：Oral presentation(general)  

Teaching Experience (On-campus)

  • Life Science of the Human Body Major achievement

    2025Year

  • Advanced lecture on Neuroscien ce

    2025Year

  • Histology (Anatomy A) Major achievement

    2025Year

  • Life Science I (Basic Medical Sciences: Physiological Regulation & Function)

    2025Year

  • Nerve-cell Biology (Lecture) Major achievement

    2025Year

  • Nerve-cell Biology (Seminar) Major achievement

    2025Year

  • Histology (Anatomy A) Major achievement

    2024Year

  • Neuroscience

    2024Year

  • Life Science I (Basic Medical Sciences: Physiological Regulation & Function)

    2024Year

  • Advanced lecture on Neuroscien ce

    2024Year

  • Introduction to Human Diseases Major achievement

    2023Year

  • Life Science I (Basic Medical Sciences: Physiological Regulation & Function)

    2023Year

  • Advanced lecture on Neuroscien ce

    2023Year

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Teaching Experience

  • 肉眼解剖学

    2025.4
    Institution：名古屋市立大学

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    Level：Undergraduate (specialized) 

  • Histology and Neuroanatomy

    2024.4

  • 肉眼解剖学及び発生学

    2023.4
    -
    2024.3

  • Histology and Neuroanatomy

    2022.4
    -
    2023.3

  • Histology

    2019.4
    -
    2022.3

  • Physiology

    2017.4
    -
    2022.3

  • Gross Anatomy

    2017.4
    -
    2020.3

  • 生物学

    2015.4
    -
    2017.3

  • 神経生理学

    Institution：大阪市立大学

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Guidance results

  • 2025

    Type：Ph.D. dissertations guidance

    Number of people receiving guidance ：1people  (Overseas students)：1people

Teaching achievements

  • 解剖学実習・組織学講義

    2023.04.01 - 2024.03.31

Review of master's and doctoral thesis

  • 2022

    Examiner classification：Second reader

    Thesis Dr. ：1people