Updated on 2024/05/01

写真a

 
Oda Toshiyuki
 
Organization
Graduate Faculty of Interdisciplinary Research Faculty of Medicine Basic Science for Clinical Medicine (Anatomy and Structural Biology) Professor
Title
Professor

Research History

  • University of Yamanashi   Professor

    2016.1

  • 東京大学大学院医学系研究科細胞生物学・解剖学講座生体構造学分野　助教

    2009.4

  • 日本学術振興会特別研究員(PD)

    2008.4

  • 日本学術振興会   特別研究員(PD)

    2008 - 2009

  • 京都大学大学院理学系研究科NEDO特別講座　委託研究生

    2007.4

  • University of Texas Southwestern Medical Center留学

    2005.4

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Education

  • The University of Tokyo

    2005.4 - 2009.3

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    Country： Japan

    Course： Doctor course

  • 京都大学大学院   理学系研究科

    2007 - 2009

  • University of Texas   Southwestern Medical Center

    2005 - 2007

  • The University of Tokyo

    2003.4 - 2005.3

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    Country： Japan

  • The University of Tokyo

    2001.4 - 2003.3

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    Country： Japan

Degree

  • Ph.D ( 2009.3   The University of Tokyo )

Research Areas

  • Life Science / Structural biochemistry

  • Life Science / Anatomy

  • Life Science / Structural biochemistry

Research Interests

  • Cryo-electron tomography

  • cilia and flagella

  • クライオ電子顕微鏡

  • 細胞生物学

  • 構造生物学

Research Projects

  • From disorder to order: mechanism of specialised assemblies formation essential for muscle function International coauthorship  Major achievement

    Grant number：RGP0062023  2023.12 - 2026.11

    Human Frontier Science Program  Human Frontier Science Program Research Grant 

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    Authorship：Coinvestigator(s)  Grant type：Competitive  Type of fund：：Others

  • クライオ電子トモグラフィーを用いたバーベック顆粒による免疫防御機構の解明

    Grant number：22H05538  2022.8 - 2024.3

    科学研究費助成事業 

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    Authorship：Principal investigator  Grant type：Competitive  Type of fund：：Science research expense

  • 肥満・糖尿病に伴う自律神経障害の病態形成メカニズムの解明と新規治療法の開発

    Grant number：22K11815  2022.4 - 2026.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    志茂 聡, 村松 憲, 大野 伸彦, 小田 賢幸

  • 公開 クライオ電子顕微鏡を用いた繊毛における分子ソート機構の解明

    2021.4 - 2024.3

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    Authorship：Principal investigator  Type of fund：：Science research expense

  • Elucidation of Molecular Sorting Mechanisms using Cryo-electron microscopy

    Grant number：21H02654  2021.4 - 2024.3

    Japan Society for the Promotion of Science  University of Yamanashi  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

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    Authorship：Principal investigator 

  • In vitro reconstitution of intraflagellar transport by cytoplasmic dynein-2

    Grant number：20K21378  2020.7 - 2022.3

    Japan Society for the Promotion of Science  Tohoku University  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    Niwa Shinsuke

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    Defects in retrograde intrafragellar transport(IFT) induces a series of diseases called ciliopathy. To understand the mechanism of ciliopathies, it is important to reconstitute intraflagellar transport in vitro. We purified recombinant cytospasmic dynein 2 from chlamydomonas. Recombinant dynein 2 was inactive because of autoinhibitory mechanisms as anticipated. To unlock the autoinhibition in vitro, we purified IFT complex from chlamydomonas as well.

  • クライオ電子顕微鏡を用いたHIV経皮・経粘膜感染防御機構の解明

    2020.4 - 2022.3

    第一三共生命科学研究振興財団  研究助成 

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    Authorship：Principal investigator  Type of fund：：Donation

  • 金結合ペプチドを用いた新規タンパク質ラベル法の開発

    2019 - 2021

    山梨大学  分野横断的融合研究プロジェクト 

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    Authorship：Principal investigator 

  • 三次元構造解析に基づく繊毛微小管の構築と繊毛運動の分子メカニズム解明

    2017.4 - 2019.3

    若手研究(A)

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    Authorship：Principal investigator  Type of fund：：Science research expense

  • 三次元構造解析に基づく繊毛微小管の構築と繊毛運動の分子メカニズム解明 研究課題

    2017.4 - 2019.3

    日本学術振興会  科学研究費補助金・若手研究(A) 

    小田賢幸

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    Authorship：Principal investigator  Grant type：Competitive 

  • 電子顕微鏡を用いた繊毛構築の分子メカニズム解明

    2017.4 - 2018.4

    上原記念生命科学財団  平成 28 年度 研究推進特別奨励金 

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    Authorship：Principal investigator  Type of fund：：Donation

  • 電子顕微鏡を用いた繊毛構築の分子メカニズム解明

    2016.11 - 2019.3

    公益財団法人千里ライフサイエンス振興財団  ２０１６年度 岸本基金研究助成 

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    Authorship：Principal investigator  Type of fund：：Donation

  • 電子顕微鏡を用いた三次元構造解析による繊毛関連疾患の病態解明

    2016.10

    公益財団法人 内藤記念科学振興財団  第４８回　内藤記念科学奨励金　研究助成 

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    Authorship：Principal investigator  Type of fund：：Donation

  • クライオ電子トモグラフィーを用いた繊毛の運動と構築メカニズムの解明

    2016.8

    公益財団法人　武田科学振興財団  2016年度 武田報彰医学研究助成 

  • クライオ電子トモグラフィーによる繊毛運動制御機構の解明

    2015.4

    公益財団法人 武田科学振興財団  2014年度 医学系研究奨励 

  • 緑藻クラミドモナスを用いた繊毛運動制御機構の解明

    2015.4 - 2017.3

    公益財団法人発酵研究所  平成27年度（2015年度）一般研究助成 

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    Authorship：Principal investigator  Type of fund：：Donation

  • 分子定規による運動性シリア構築メカニズムの解明

    2015.4 - 2017.3

    新学術領域研究(研究領域提案型)

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    Authorship：Principal investigator  Type of fund：：Science research expense

  • クライオ電子顕微鏡による繊毛内メカノシグナリングの解析

    2014.4 - 2016.3

    公益財団法人　風戸研究奨励会  第七回（平成25年度）風戸研究奨励賞 

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    Authorship：Principal investigator  Type of fund：：Donation

  • 鞭毛ダイニン中間鎖の構造と機能の解析

    2011 - 2012

    若手研究(B)

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    Authorship：Principal investigator  Type of fund：：Science research expense

  • 鞭毛ダイニン中間鎖の構造と機能の解析 研究課題

    2011 - 2012

    日本学術振興会  科学研究費補助金・若手研究(B) 

    小田賢幸

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    Authorship：Principal investigator  Grant type：Competitive 

  • クライオ電子顕微鏡を用いたダイニン-微小管複合体の構造解析

    2008 - 2009

    特別研究員奨励費

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    Authorship：Principal investigator  Type of fund：：Science research expense

  • クライオ電子顕微鏡を用いたダイニン-微小管複合体の構造解析 研究課題

    2008 - 2009

    日本学術振興会  科学研究費補助金・特別研究員奨励費 

    小田賢幸

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    Authorship：Principal investigator  Grant type：Competitive 

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Papers

  • Cryo-EM elucidates the uroplakin complex structure within liquid-crystalline lipids in the porcine urothelial membrane Reviewed Major achievement

    Haruaki Yanagisawa, Yoshihiro Kita, Toshiyuki Oda, Masahide Kikkawa

    Communications Biology   2023.10

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s42003-023-05393-x

  • Biological and Medical Applications of Cross-scale Measurements Reviewed

    58 ( 2 )   60 - 65   2023.8

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    Language：Japanese   Publishing type：Research paper (scientific journal)  

    DOI： 10.11410/kenbikyo.58.2_60

  • α- and β-tubulin C-terminal tails with distinct modifications are crucial for ciliary motility and assembly Reviewed

    Tomohiro Kubo, Yuma Tani, Haru-Aki Yanagisawa, Masahide Kikkawa, Toshiyuki Oda

    Journal of Cell Science   2023.7( ISSN：0021-9533  eISSN：1477-9137 )

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Company of Biologists  

    α- and β-tubulin have an unstructured glutamate-rich region at their C-terminal tails (CTT). The function of this region in cilia/flagella is still unclear, except that glutamates in CTT act as the sites for posttranslational modifications that affect ciliary motility. A unicellular alga Chlamydomonas possesses only two a-tubulin genes and two b-tubulin genes, each pair encoding an identical protein. This simple gene organization may enable a complete replacement of the wild-type tubulin with its mutated version. Here, using CRISPR/Cas9, we generated mutants expressing tubulins with modified CTTs. We found that the mutant whose four glutamate residues in the α-tubulin CTT have been replaced by alanine almost completely lacked polyglutamylated tubulin and displayed paralyzed cilia. In contrast, the mutant lacking the glutamate-rich region of the β-tubulin CTT assembled short cilia without the central apparatus. This phenotype is similar to the mutants harboring a mutation in a subunit of katanin, whose function has been shown to depend on the b-tubulin CTT. Therefore, our study reveals distinct and important roles of α- and β-tubulin CTT in the formation and function of cilia.

    DOI： 10.1242/jcs.261070

  • Distinct roles of α- and β-tubulin C-terminal tails for ciliary function as revealed by a CRISPR/Cas9 mediated gene editing in Chlamydomonas Reviewed

    Tomohiro Kubo, Yuma Tani, Haruaki Yanagisawa, Masahide Kikkawa, Toshiyuki Oda

    2023.2

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    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1101/2023.02.14.528553

  • Cryo-electron tomography of Birbeck granules reveals the molecular mechanism of langerin lattice formation Reviewed Major achievement

    Toshiyuki Oda, Haruaki Yanagisawa, Hideyuki Shinmori, Youichi Ogawa, Tatsuyoshi Kawamura

    eLife   2022.6( ISSN：2050-084X )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.7554/eLife.79990

  • Puromycin is incorporated into regenerating flagella of Chlamydomonas reinhardtii as an indicator of nascent flagellar proteins

    Tomohiro Kubo, Natsumi Kanou, Toshiyuki Oda

    2022.1

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    Language：English   Publishing type：(MISC) Institution technical report and pre-print, etc.   Publisher：Cold Spring Harbor Laboratory  

    <title>Abstract</title>The unicellular alga <italic>Chlamydomonas reinhardtii</italic> assembles flagella after pH shock-induced deflagellation, in which process the cell vigorously synthesizes flagellar proteins. Previously, newly synthesized flagellar proteins were chased using radioisotopes. Here, we have developed an alternative, non-radioactive method using the surface sensing of translation (SUnSET) assay, an assay that takes advantage of the incorporation of the antibiotic puromycin into newly synthesized peptides. Just after deflagellation, puromycin-labeled proteins increased dramatically in the cytoplasm and newly assembled flagella. Axonemal incorporation of newly synthesized proteins occurred from the distal tip of the flagellum. Cycloheximide, a protein synthesis inhibitor, almost completely prevented the cellular and flagellar incorporation of puromycin, although, as reported previously, it allowed reassembly of half-length flagella from the cytoplasmic “flagellar precursor” proteins. In contrast to wild-type cells, a cycloheximide-resistant mutant <italic>act2</italic> produced nearly full-length flagella containing puromycin-labeled proteins even in the presence of cycloheximide. These and other results demonstrate that the SUnSET method serves as a powerful tool for visualizing flagellar incorporation of nascent proteins in <italic>Chlamydomonas.</italic>

    DOI： 10.1101/2022.01.27.478094

  • Chlamydomonas FAP70 is a component of the previously uncharacterized ciliary central apparatus projection C2a. Reviewed

    Yuqing Hou, Lei Zhao, Tomohiro Kubo, Xi Cheng, Nathan McNeill, Toshiyuki Oda, George B Witman

    JOURNAL OF CELL SCIENCE   134 ( 12 )   2021.6( ISSN：0021-9533 )

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/jcs.258540

  • Neural and behavioral control in Caenorhabditis elegans by a yellow-light-activatable caged compound Reviewed Major achievement

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   2021.2( ISSN：0027-8424 )

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.2009634118

    PubMed

  • Cryo-electron tomography of cardiac myofibrils reveals a 3D lattice spring within the Z-discs Reviewed

    Toshiyuki Oda, Haruaki Yanagisawa

    Communications Biology   2020.10

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s42003-020-01321-5

    PubMed

  • TIM, a targeted insertional mutagenesis method utilizing CRISPR/Cas9 in Chlamydomonas reinhardtii. Reviewed

    PLoS One   2020.5( ISSN：1932-6203 )

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin Reviewed

    JOURNAL OF STRUCTURAL BIOLOGY   2020.3( ISSN：1047-8477 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  • 光子と電子による神経機能と構造の解析

    高橋光規, 神谷真子, 河谷稔, 梅澤啓太郎, 浦野泰照, 丹羽伸介, 小田賢幸

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   125th   2020

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    Authorship：Corresponding author   Language：Japanese   Publishing type：Research paper (international conference proceedings)  

    J-GLOBAL

  • Disease-associated mutations hyperactivate KIF1A motility and anterograde axonal transport of synaptic vesicle precursors. Reviewed

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   2019.9( ISSN：0027-8424 )

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    Language：English   Publishing type：Research paper (scientific journal)  

    PubMed

  • 新規ケージド化合物による線虫神経活動の光制御

    高橋光規, 神谷真子, 河谷稔, 梅澤啓太郎, 丹羽伸介, 小田賢幸, 浦野泰照

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   124th   145   2019

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    Authorship：Corresponding author   Language：Japanese   Publishing type：Research paper (international conference proceedings)  

    J-GLOBAL

  • Interactions between mitochondria and endoplasmic reticulum in demyelinated axons Reviewed

    Medical Molecular Morphology   2018.11( ISSN：1860-1480 )

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Chlamydomonas as a tool to study tubulin polyglutamylation. Invited Reviewed

    Microscopy   2018.10( ISSN：2050-5698 )

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    Language：English   Publishing type：Research paper (scientific journal)  

  • CFAP70 Is a Novel Axoneme-Binding Protein That Localizes at the Base of the Outer Dynein Arm and Regulates Ciliary Motility. Reviewed

    Cells   2018.8( ISSN：2073-4409 )

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Decreased number and increased volume with mitochondrial enlargement of cerebellar synaptic terminals in a mouse model of chronic demyelination Reviewed

    Medical Molecular Morphology   2018.5( ISSN：1860-1480 )

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    Language：English   Publishing type：Research paper (scientific journal)  

  • Systematic studies of all PIH proteins in zebrafish reveal their distinct roles in axonemal dynein assembly. Reviewed

    eLife   2018.5( ISSN：2050-084X )

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    Language：English   Publishing type：Research paper (scientific journal)  

  • A microtubule-dynein tethering complex regulates the axonemal inner dynein f (I1) Reviewed

    Tomohiro Kubo, Yuqing Hou, Deborah A. Cochran, George B. Witman, and Toshiyuki Oda

    MOLECULAR BIOLOGY OF THE CELL   2018( ISSN：1059-1524 )

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1091/mbc.E17-11-0689

    PubMed

  • クライオ電子トモグラフィーの実用技術 Invited Reviewed

    小田賢幸

    顕微鏡   53 ( 1 )   2018

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    Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese  

  • 線虫神経シナプスの電子顕微鏡観察

    高橋光規, 丹羽伸介, 小田賢幸

    日本解剖学会総会・全国学術集会講演プログラム・抄録集   123rd   183   2018

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    Authorship：Corresponding author   Language：Japanese   Publishing type：Research paper (international conference proceedings)  

    J-GLOBAL

  • Electrostatic interaction between polyglutamylated tubulin and the nexin-dynein regulatory complex regulates flagellar motility. Reviewed

    Tomohiro Kubo, Toshiyuki Oda

    MOLECULAR BIOLOGY OF THE CELL   2017.6( ISSN：1059-1524 )

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    Authorship：Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1091/mbc.E17-05-0285

    PubMed

  • Three-dimensional structural labeling microscopy of cilia and flagella Reviewed

    Toshiyuki Oda

    Microscopy   2017.5( ISSN：0022-0744 )

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    Authorship：Lead author,　Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/jmicro/dfx018

  • Structure and function of outer dynein arm intermediate and light chain complex. Reviewed

    Oda T, Abe T, Yanagisawa H, Kikkawa M

    MOLECULAR BIOLOGY OF THE CELL   27   1051 - 1059   2016( ISSN：1059-1524 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  • Docking-complex-independent alignment of Chlamydomonas outer dynein arms with 24-nm periodicity in vitro. Reviewed

    Oda T, Abe T, Yanagisawa H, Kikkawa M

    JOURNAL OF CELL SCIENCE   129   1547 - 1551   2016( ISSN：0021-9533 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  • Detailed structural and biochemical characterization of the nexin-dynein regulatory complex. Reviewed

    Oda T, Yanagisawa H, Kikkawa M

    MOLECULAR BIOLOGY OF THE CELL   26   294 - 304   2015( ISSN：1059-1524 )

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

  • How Do Cells Measure Length? Reviewed

    Masahide KIKKAWA, Toshiyuki ODA, Haruaki YANAGISAWA

    Seibutsu Butsuri   55 ( 5 )   250 - 254   2015( ISSN：0582-4052 )

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    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Biophysical Society of Japan  

    Existence of cellular structures with specific size and length raises a fundamental question in biology: how do cells measure length? One conceptual answer to this question is a molecular ruler, but examples of such rulers in eukaryotes have been lacking. We recently identified a molecular ruler in eukaryotic cilia and flagella using genetic screening and cryo-electron tomography. We demonstrated that FAP59 and FAP172 form a 96-nm-long complex in Chlamydomonas flagella and the absence of the complex disrupted 96-nm repeats of axonemes. Furthermore, lengthening of the FAP59/172 complex resulted in extension of the repeats up to 128 nm.

    DOI： 10.2142/biophys.55.250

    CiNii Books

  • The conserved ciliary protein Bug22 controls planar beating of Chlamydomonas flagella Reviewed International coauthorship

    Dan Meng, Muqing Cao, Toshiyuki Oda, Junmin Pan

    JOURNAL OF CELL SCIENCE   127 ( 2 )   281 - 287   2014.1( ISSN：0021-9533  eISSN：1477-9137 )

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：COMPANY OF BIOLOGISTS LTD  

    Eukaryotic flagella and cilia can exhibit planar and non-planar beating, and the mechanism controlling these beating patterns is not well understood. Chlamydomonas reinhardtii flagella beat in approximately the same plane with either an asymmetric ciliary-type or symmetric flagellar-type waveform. Each B-tubule of the number 1, 5 and 6 doublets of the flagellar axoneme possesses a beak-like structure. The number 5 and 6 beak structures are implicated in conversion of ciliary motion into flagellar motion. Here, we show that in a null mutant of Bug22, the asymmetric ciliary waveform is converted into a three-dimensional (non-planar) symmetric flagellar waveform. Bug22 is localized to approximately the proximal half to two-thirds of the flagellum, similar to localization of beak-like structures. However, as shown by immunogold labeling, Bug22 associates with axonemal microtubules without apparent preference for any particular doublets. Interestingly, bug22 mutants lack all beaklike structures. We propose that one function of Bug22 is to regulate the anchoring of the beak-like structures to the doublet microtubules and confine flagellar beating to a plane.

    DOI： 10.1242/jcs.140723

    Web of Science

    PubMed

  • Mechanosignaling between central apparatus and radial spokes controls axonemal dynein activity. Reviewed Major achievement

    Oda T, Yanagisawa H, Yagi T, Kikkawa M

    JOURNAL OF CELL BIOLOGY   204   807 - 819   2014( ISSN：0021-9525 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  • A molecular ruler determines the repeat length in eukaryotic cilia and flagella. Reviewed Major achievement

    Oda T, Yanagisawa H, Kamiya R, Kikkawa M

    SCIENCE   346   857 - 860   2014( ISSN：0036-8075 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  • Identification of the outer-inner dynein linker as a hub controller for axonemal dynein activities. Reviewed Major achievement

    Oda T, Yagi T, Yanagisawa H, Kikkawa M

    CURRENT BIOLOGY   2013( ISSN：0960-9822 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  • Novel structural labeling method using cryo-electron tomography and biotin-streptavidin system. Reviewed

    Oda T, Kikkawa M

    JOURNAL OF STRUCTURAL BIOLOGY   183   305 - 311   2013( ISSN：1047-8477 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  • クラミドモナスを用いた鞭毛運動の多角的解析 –電子顕微鏡から細胞生物学まで- Invited Reviewed

    小田賢幸

    顕微鏡   48   94 - 99   2013

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    Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

  • Three-dimensional structures of the flagellar dynein–microtubule complex by cryoelectron microscopy Reviewed

    Toshiyuki Oda, Nobutaka Hirokawa, Masahide Kikkawa

    The Journal of Cell Biology   177 ( 2 )   243 - 252   2007.4( ISSN：0021-9525 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Rockefeller University Press  

    DOI： 10.1083/jcb.200609038

    PubMed

  • Three-dimensional structures of the flagellar dynein-microtubule complex by cryoelectron microscopy. Reviewed Major achievement

    Oda T, Hirokawa N, Kikkawa M

    JOURNAL OF CELL BIOLOGY   177   243 - 252   2007( ISSN：0021-9525 )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  • Ligand stimulation of CD155alpha inhibits cell adhesion and enhances cell migration in fibroblasts. Reviewed

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   319 ( 4 )   1253 - 1264   2004.7( ISSN：0006-291X )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    PubMed

  • Receptor (CD155)-dependent endocytosis of poliovirus and retrograde axonal transport of the endosome Reviewed

    S Ohka, N Matsuda, K Tohyama, T Oda, M Morikawa, S Kuge, A Nomoto

    JOURNAL OF VIROLOGY   78 ( 13 )   7186 - 7198   2004.7( ISSN：0022-538X )

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC MICROBIOLOGY  

    Poliovirus (PV), when injected intramuscularly into the calf, is incorporated into the sciatic nerve and causes an initial paralysis of the inoculated limb in transgenic mice carrying the human PV receptor (hPVR/CD155) gene. Here, we demonstrated by using an immunoelectron microscope that PV particles exist on vesicle structures in nerve terminals of neuromuscular junctions. We also demonstrated in glutathione S-transferase pull-down experiments that the dynein light chain, Tctex-1, interacts directly with the cytoplasmic domain of hPVR. In the axons of differentiated rat PC12 cells transfected with expression vectors for hPVRs, vesicles composed of PV and hPVRalpha, as well as a mutant hPVRalpha (hPVRMalpha) that had a reduced ability to bind Tctex-1, co-localized with Tctex-1. However, vesicles containing PV, dextran, and hPVRalpha had only retrograde motion, while those containing PV, dextran, and hPVRMalpha had anterograde or retrograde motion. Topical application of the antimicrotubule agent vinblastine to the sciatic nerve reduced the amount of virus transported from the calf to the spinal cord. These results suggest that direct efficient interaction between the cytoplasmic domain and Tctex-1 is essential for the efficient retrograde transport of PV-containing vesicles along microtubules in vivo.

    DOI： 10.1128/JVI.78.13.7186-7198.2004

    Web of Science

    PubMed

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Presentations

  • クライオ電子トモグラフィーによるバーベック顆粒の三次元構造解析 International conference

    顕微鏡学会第78回学術講演会  2022.5 

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    Event date： 2022.5

    Language：Japanese   Presentation type：Oral presentation(general)  

  • Cryo-electron tomography of cardiac myofibrils reveals a 3D lattice spring within the Z-discs Invited International conference

    2021.6 

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    Event date： 2021.6

    Language：English   Presentation type：Oral presentation(invited, special)  

  • Molecular basis of periodicity within macromolecular complexes Invited

    2021.3 

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    Event date： 2021.3

    Language：English   Presentation type：Oral presentation(invited, special)  

  • Cryo-electron tomography of cardiac Z-disc Invited

    2020.5 

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    Event date： 2020.5

    Language：English   Presentation type：Oral presentation(invited, special)  

  • Cryo-EM structures of cardiac thin filament revealed by calcium-dependent mechanism of tropomyosin shift by troponin Invited

    Toshiyuki Oda, Haruaki Yanagisawa, TakeyukWakabayashi

    2020.3 

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    Language：Japanese  

  • クライオ電顕による心筋収縮装置の三次元構造解析 Invited International conference

    第42回日本分子生物学会年会  2019.12 

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    Event date： 2019.12

    Language：Japanese   Presentation type：Oral presentation(invited, special)  

  • Single particle analysis of cardiac thin filament using Volta phase plate Invited

    2019.6 

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    Event date： 2019.6

    Language：English   Presentation type：Oral presentation(invited, special)  

  • Application of Cryo-electron Tomography to Histology Invited International conference

    The 75th Annual Meeting of the Japanese Society of Microscopy  2019.6 

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    Event date： 2019.6

    Language：English   Presentation type：Oral presentation(invited, special)  

  • 3D structural analysis of cilia using cryo-electron tomography Invited International conference

    8th Asia Pacific International Congress of Anatomists  2018.11 

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    Event date： 2018.11

    Language：English   Presentation type：Oral presentation(invited, special)  

  • クライオ電顕の試料作成とデータ解析 Invited

    電顕サマースクール  2018.8 

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    Event date： 2018.8

    Language：Japanese   Presentation type：Oral presentation(invited, special)  

    Venue：信州大学  

  • クライオ電顕の試料作製とデータ解析 Invited

    小田賢幸

    平成３０年度電顕サマースクール  2018.8 

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    Language：Japanese   Presentation type：Oral presentation(invited, special)  

  • クライオ電子トモグラフィーによる繊毛モーターおよびチューブリン修飾の構造解析 Invited

    生理学研究所プロジェクト「機能タンパク質の構造と機能のダイナミクスと、 それに基づく細胞・生体システム作動機構の研究拠点の形成」平成 29 年度末 シンポジウム  2018.3 

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    Event date： 2018.3

    Language：Japanese   Presentation type：Oral presentation(invited, special)  

  • クライオ電子トモグラフィーによる繊毛モーターおよびチューブリン修飾の構造解析 Invited

    小田賢幸

    機能タンパク質の構造と機能のダイナミクスと、それに基づくく細胞・生体システム作動機構の研究拠点の形成 平成29年度末 シンポジウム  2018 

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    Language：Japanese  

  • Electrostatic interaction between polyglutamylated tubulin and the nexin-dynein regulatory complex regulates flagellar motility Invited International conference

    Dynein 2017  2017.11 

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    Event date： 2017.11

    Language：English   Presentation type：Oral presentation(invited, special)  

  • クライオ電子トモグラフィーが明らかにした繊毛の運動と構築の分子メカニズム Invited

    小田賢幸

    日本細胞生物学会第６７回大会  2015.7 

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    Language：Japanese  

  • Identification of the molecular ruler that defines the 96-nm repeats of cilia/flagella Invited International conference

    Toshiyuki Oda

    American Society of Cell Biology Annual Meeting  2014.12 

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    Language：English   Presentation type：Oral presentation(invited, special)  

  • A molecular ruler determines the 96-nm repeats of cilia/flagella. Invited International conference

    小田賢幸

    7th International Electron Tomography Conference  2014.11 

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    Language：English  

  • 10. Discovery of a molecular ruler in eukaryotic cilia/flagella Invited

    小田賢幸

    Opening International Symposium Next-Generation Microscopic Science  2014.11 

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    Language：English  

  • 10. クライオ電子トモグラフィーを用いた構造ラベリング Invited

    小田賢幸

    日本顕微鏡学会第７０回学術記念講演会  2014.5 

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    Language：Japanese  

  • クライオ電子顕微鏡で迫る繊毛運動制御機構 Invited

    小田賢幸

    日本顕微鏡学会第５７回シンポジウム  2013.11 

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    Language：Japanese  

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Awards

  • 風戸研究奨励賞

    2014.3   公益財団法人　風戸研究奨励会  

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    Award type：Award from publisher, newspaper, foundation, etc. 

  • 第七回（平成25年度）風戸研究奨励賞

    公益財団法人 風戸研究奨励会  

    小田賢幸

Teaching Experience (On-campus)

  • Histology (Anatomy A) Major achievement

    2023Year

  • Introduction to Human Morphology and Function

    2023Year

  • Life Science of the Human Body Major achievement

    2023Year

  • Advanced lecture on Neuroscien ce

    2023Year

  • Introduction to Human Diseases

    2023Year

Teaching Experience

  • 人体発生学

    2016
    Institution：山梨大学医学部

  • 骨学

  • 神経解剖学

Guidance results

  • 2021

    Type：Master's （Major B course）dissertations guidance

    Number of people receiving guidance ：1people 

  • 2020

    Type：Achievement of student guidance (graduate school)

    Number of people receiving guidance ：1people 

  • 2018

    Type：Ph.D. dissertations guidance

    Number of people receiving guidance ：2people  (Overseas students)：2people

    Graduation / pass / number of people awarded degrees ：2people  (Overseas students)：2people

Social Activities

  • ＪＳＴ事業「女子中高生の理系進路選択支援 プログラム」附属中学校 出前講義

    Role(s)： Lecturer

    山梨大学男女共同参画推進室  2018.10

  • 高校生向けサイエンスフォーラム

    Role(s)： Lecturer

    山梨大学医学部リエゾンアカデミー  2018.8

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    Audience： High school students

    Type：Lecture

  • Acceptance of students from Yamanashi prefectural School for the Visually Impaired (Dissection practice tour)

    2018.6

  • 未来の科学者訪問セミナー

    山梨科学アカデミー事務局　山梨県私学・科学振興課  2017.11

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    Audience： Junior students

    Type：Lecture

  • 未来の科学者訪問セミナー

    Role(s)： Lecturer

    笛吹市立一宮中学校  2017.11

  • Acceptance of students from Yamanashi prefectural School for the Visually Impaired (Dissection practice tour)

    2017.6

  • Acceptance of students from Yamanashi prefectural School for the Visually Impaired (Dissection practice tour)

    2016.6

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Professional Memberships

  • THE JAPANESE SOCIETY OF MICROSCOPY

  • THE JAPANESE ASSOCIATION OF ANATOMISTS