Organization
Directors Executive Director
Title
Executive Director
Contact information
Kurosawa Hiroshi
Research History
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University of Yamanashi Professor
2012.4
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University of Yamanashi Professor, Graduate School of Medicine and Engineering, University of Yamanashi Professor
2009.10 - 2012.3
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University of Yamanashi Associate professor, Graduate School of Medicine and Engineering, University of Yamanashi Associate Professor
2007.4 - 2009.9
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University of Yamanashi
2003.4 - 2006.3
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University of Yamanashi
1995.12 - 2003.3
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University of Yamanashi Senior Assistant Professor
1992.4 - 1995.11
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University of Yamanashi Research Associate
1990.4 - 1992.3
Education
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University of Tsukuba Graduate School of Agriculture, University of Tsukuba
1986.4 - 1989.3
Country： Japan
Course： Doctor course
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University of Tsukuba
1984.4 - 1986.3
Country： Japan
Course： Master course
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University of Tsukuba Faculty of Agriculture, University of Tsukuba
1980.4 - 1984.3
Country： Japan
Degree
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ph.D. ( 1989.3 University of Tsukuba )
Current state of research and teaching activities
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「再生医療の実用化を目指した生物工学的研究」
iPS細胞やES細胞などの多能性幹細胞について培養工学の観点から研究を行っています。多能性幹細胞から効率よく目的細胞をつくり出すために、細胞の増やし方（増殖法）や心筋などの機能細胞に変化させる技術（分化誘導法）の開発を行っています。また、再生医療の実用化へ向けて細胞の品質評価に関する研究を行っています。
Research Areas
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Life Science / Applied microbiology / Applied Microbiology
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Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering / Cell culture engineering Biochemical engineering
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Life Science / Biomedical engineering / Tissue Engineering
Research Interests
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Embryonic stem cells
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Tissue Engineering
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Cell Culture Engineering
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Biochemical Engineering
Subject of research
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Standardization of embryoid body formation.
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Bioengineering study for practical application of regenerative medicine.
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Control of the differentiation of pluripotent stem cells.
Papers
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TMEM180 contributes to SW480 human colorectal cancer cell proliferation through intra-cellular metabolic pathways. Reviewed
Translational Oncology 14 2021.7( ISSN：1936-5233 )
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Optimized conditions for the supplementation of human-induced pluripotent stem cell cultures with a GSK-3 inhibitor during embryoid body formation with the aim of inducing differentiation into mesodermal and cardiac lineage. Reviewed Major achievement
Kiyomi Yoda, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 129 ( 3 ) 371 - 378 2020.3( ISSN：1389-1723 )
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Optimization of the treatment conditions with glycogen synthase kinase-3 inhibitor towards enhancing the proliferation of human induced pluripotent stem cells while maintaining an undifferentiated state under feeder-free conditions Reviewed Major achievement
Kiyomi Yoda, Yoshitsugu Ohnuki, Hiroshi Kurosawa
JOURNAL OF BIOSCIENCE AND BIOENGINEERING 127 ( 3 ) 381 - 387 2019.3( ISSN：1389-1723 )
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ROCK inhibitor Y-27632 used during cell aggregate formation adversely affects the early cardiac differentiation on human iPS cells. Reviewed
Yoshitsugu Ohnuki, Takumi Kondo, and Hiroshi Kurosawa.
Tissue Culture Research Communications 36 21 - 29 2017.5
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Differential X chromosome inactivation patterns during the propagation of human induced pluripotent stem cells Reviewed
Tomoko Andoh-Noda, Wado Akamatsu, Kunio Miyake, Tetsuro Kobayashi, Manabu Ohyama, Hiroshi Kurosawa, Takeo Kubota, and Hideyuki Okano
The Keio Journal of Medicine 66 ( 1 ) 1 - 8 2017.3
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Functional Neurons Generated from T Cell-Derived Induced Pluripotent Stem Cells for Neurological Disease Modeling. Reviewed
Takuya Matsumoto, Koki Fujimori, Tomoko Andoh-Noda, Takayuki Ando, Naoko Kuzumaki, Manabu Toyoshima, Hirobumi Tada, Kent Imaizumi, Mitsuru Ishikawa, Ryo Yamaguchi, Miho Isoda, Zhi Zhou, Shigeto Sato, Tetsuro Kobayashi, Manami Ohtaka, Ken Nishimura, Hiroshi Kurosawa, Takeo Yoshikawa, Takuya Takahashi, Mahito Nakanishi, Manabu Ohyama, Nobutaka Hattori, Wado Akamatsu, and Hideyuki Okano
Stem Cell Reports 2016( ISSN：2213-6711 )
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Effective Rho-associated protein kinase inhibitor treatment to dissociate human iPS cells for suspension culture to form embryoid body-like cell aggregates. Reviewed
Ayumi Horiguchi, Koyuki Yazaki, Mami Aoyagi, Yoshitsugu Ohnuki, and Hiroshi Kurosawa
Journal of Bioscience and Bioengineering 2014( ISSN：1389-1723 )
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Comparison of Genomic and Epigenomic Expression in Monozygotic Twins Discordant for Rett Syndrome. Reviewed
Miyake K, Yang C, Minakuchi Y, Ohori K, Soutome M, Hirasawa T, Kazuki Y, Adachi N, Suzuki S, Itoh M, Goto YI, Andoh T, Kurosawa H, Akamatsu W, Ohyama M, Okano H, Oshimura M, Sasaki M, Toyoda A, Kubota T.
PLoS One 2013.6( ISSN：1932-6203 )
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Effects of hanging drop culture conditions on embryoid body formation and neuronal cell differentiation using mouse embryonic stem cells: Optimization of culture conditions for the formation of well-controlled embryoid bodies. Reviewed Major achievement
Y. Ohnuki and H. Kurosawa
Journal of Bioscience and Bioengineering 115 ( 5 ) 571 - 574 2013.5( ISSN：1389-1723 )
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Application of Rho-associated protein kinase (ROCK) inhibitor to human pluripotent stem cells Reviewed Major achievement
H. Kurosawa
Journal of Bioscience and Bioengineering 114 ( 6 ) 577 - 581 2012.12( ISSN：1389-1723 )
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Effect of glucose concentration during embryoid body (EB) formation from mouse embryonic stem cells on EB growth and cell differentiation. Reviewed Major achievement
H. Mochizuki, ,Y. Ohnuki, ,H. Kurosawa
Journal of Bioscience and Bioengineering 111 ( 1 ) 92 - 97 2011.1( ISSN：1389-1723 )
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低細胞付着性培養器材による胚様体形成の効率化 Reviewed Major achievement
黒澤尋
再生医療 8 ( 3 ) 345 - 351 2009.8
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Development of cystic embryoid bodies with visceral yolk-sac-like structures from mouse embryonic stem cells using low-adherence 96-well Reviewed Major achievement
E. Yasuda,Y. Seki,T. Higuchi,F. Nakashima,T. Noda,H. Kurosawa
Journal of Bioscience and Bioengineering 107 ( 4 ) 442 - 446 2009.4
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Characterization of Embryoid Bodies of Mouse Embryonic Stem Cells Formed under Various Culture Conditions and Estimation of Differentiation Status of Such Bodies Reviewed Major achievement
Mikiko Koike,Shujiro Sakaki,Yoshifumi Amano,Hiroshi Kurosawa
Journal of Bioscience and Bioengineering 104 ( 4 ) 294 - 299 2007.11
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Methods for inducing embryoid body formation: In vitro differentiation system of embryonic stem cells. Reviewed Major achievement
Hiroshi Kurosawa
Journal of Bioscience and Bioengineering 103 ( 5 ) 389 - 398 2007.5
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Effect of oxygen on in vitro differentiation of mouse embryonic stem cells Reviewed Major achievement
H. Kurosawa ,M. Kimura,T. Noda,Y. Amano
Journal of Bioscience and Bioengineerin 101 ( 1 ) 26 - 30 2006.1
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A round-bottom 96-well polystyrene plate coated with 2-methacryloyloxyethyl phosphorylcholine as an effective tool for embryoid body formation Reviewed Major achievement
M. Koike,H. Kurosawa,Y. Amano
Cytotechnology 47 ( 1-3 ) 3 - 10 2005.12
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Enhancement of albumin secretion by short-term hypothermic incubation of primary rat hepatocyte Reviewed
M. Katsuki,H. Kurosawa,Y. Amano
Biochemical Engineering Journal 20 137 - 141 2004.8
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摘出肝臓を用いた高酸素親和性人工酸素運搬体（Neo Red Cell）の酸素運搬能の評価 Reviewed
木村哲寛,黒澤 尋,緒方嘉貴
人工血液 11 ( 3 ) 179 - 184 2003.11
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A simple method for forming embryoid body from mouse embryonic stem cells Reviewed Major achievement
H. Kurosawa,T. Imamura,M. Koike,K. Sasaki, Y. Amano
Journal of Bioscience and Bioengineering 96 ( 4 ) 409 - 411 2003.10
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Morphology and albumin secretion of adult rat hepatocytes cultured on a hydrophobic porous expanded polytetrafluoroethylene membrane Reviewed
H. Kurosawa,E. Yuminamochi,R. Yasuda,Y. Amano
Journal of Bioscience and Bioengineering 95 ( 1 ) 59 - 64 2003.1
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Adult rat hepatocytes cultured on an oxygen-permeable film increases the activity of albumin secretion Reviewed
H. Kurosawa,R. Yasuda,Y. Osano,Y. Amano
Cytotechnology 36 ( 1-3 ) 85 - 92 2001.12
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Polyurethane membrane as an efficient immobilization carrier for high-density culture of rat hepatocytes in the fixed-bed reactor. Reviewed
H. Kurosawa,K. Yasumoto,T. Kimura,Y. Amano
Biotechnology and Bioengineering 70 ( 2 ) 160 - 166 2000.10
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Cultivation of rat hepatocytes in a medium based on commercially available infusion solutions Reviewed
H. Kurosawa,M. Chino,Y. Amano
Journal of Bioscience and Bioengineering 89 ( 6 ) 615 - 616 2000.6
Books and Other Publications
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第9章 動物細胞培養系における酸素供給と酸素消費 Major achievement
黒澤 尋（ Role： Joint Work）
シーエムシー出版 2021.11 （ ISBN:978-4-7813-1619-2 ）
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酸素供給の重要性 Major achievement
黒澤 尋（-）
株式会社エヌ・ティー・エス 2014.6
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培養細胞への酸素供給 Major achievement
黒澤 尋（91-11）
日本生物工学会 2013.12
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さまざまな培養条件下で形成したマウスES細胞胚様体の分化状態の特徴付け Major achievement
小池 美紀子,榊 秀次郎,天野 義文,黒澤 尋（-）
（社）日本生物工学会 2009.2
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環太平洋生物化学工学国際会議における医工・産官学連携シンポジウム開催に関する調査 国内および国外におけるセル＆ティッシュエンジニアリング研究の現状
研究代表者 高木 睦（-）
2007.3
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バイオミメティック組織デバイス 開発の現状と将来展望 Major achievement
社団法人 日本生物工学会,セル＆ティッシュエンジニアリング研究部会 編（-）
三恵社 2004.3
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再生医療実用化にむけた生物工学研究 米英および国内生物工学者の活動 Major achievement
社団法人 日本生物工学会,セル＆ティッシュエンジニアリング研究部会 編（-）
三恵社 2003.3
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生物工学実験書 改訂版 Major achievement
日本生物工学会編（-）
培風館 2002.4
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Primary culture of rat hepatocytes on an oxygen permeable membrane Major achievement
H. Kurosawa,R. Yasuda,Y. Amano（-）
Kluwer Academic 2002.4
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ティッシュエンジニアリングの展望ー再生医療関連の産業化動向・日米比較
社団法人 日本生物工学会,セル＆ティッシュエンジニアリング研究部会編（-）
三恵社 2002.3
Presentations
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Novel Cyclic N-alkyl Peptide Dimers as bFGF Mimetics to Maintain thePluripotency of Human Induced Pluripotent Stem Cells
Masashi Sato, Yoshitsugu Ohnuki, Shinji Masui, Takashi Kawakami and Hiroshi Kurosawa
2021.9
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Screening of molecule that facilitate transition from mouse embryonic stem cells to early-embryonic-like cells. International conference
Aoi Murakami, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa
JAACT2020 Fuchu 2020.11
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Primitive endoderm cells promote proliferation and differentiation of pluripotent stem cells. International conference
Takumi Iwama, Yoshitsugu Ohnuki, Shinji Masui, Hiroshi Kurosawa
JAACT2020 Fuchu 2020.11
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Novel cyclic N-alkyl peptides as bFGF mimetics to maintain the pluripotency of human induced pluripotent stem cells. International conference
M. Sato, H. Tanaka, Y. Ohnuki, S. Masui, T. Kawakami, H. Kurosawa
JAACT2020 Fuchu 2020.11
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GSK-3b inhibitor treatment for human iPS cells to form mesoderm differentiation-promoted embryoid bodies.
2019.7
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胚様体形成期間中のCHIR99021処置濃度がヒトiPS細胞の心筋細胞分化誘導に与える影響
第70回日本生物工学会大会 2018.9
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GSK-3β阻害剤CHIR99021がヒトiPS細胞の増殖性及び分化方向性に及ぼす影響
日本組織培養学会 第91回大会 2018.6
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ヒトiPS細胞から心筋細胞を分化誘導する過程における心筋分化関連遺伝子の発現量の変動
日本組織培養学会 第94回大会 2018.6
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ヒトiPS細胞の胚様体を構成する細胞数を求める方法の検討
三浦汐織,大貫喜嗣,黒澤尋
第65回日本生物工学会大会講演要旨集 2013.9
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ヒトiPS細胞から神経系細胞を経由して脂肪細胞を分化誘導する条件の検討
藤田紗代,大貫喜嗣,望月和樹,黒澤尋
第65回日本生物工学会大会講演要旨集 2013.9
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Effective use of ROCK inhibitor (Y-27632) for reproducible manipulations in the research of human iPS cells. Major achievement
The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT 2012) Program & Abstracts 2012.11
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ROCK inhibitor (Y-27632)をヒトiPS細胞の分散処理時に添加することの有効性
堀口あゆみ,大貫喜嗣,青柳満味,黒澤 尋
第64回日本生物工学会大会講演要旨集 2012.10 日本生物工学会
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ROCK inhibitor (Y-27632) の存在がヒトiPS細胞の胚様体形成に及ぼす影響
矢崎こゆき,青柳満味,大貫喜嗣,黒澤 尋
再生医療 2012.6
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シングルセル化したヒトiPS細胞からの胚様体形成とその分化状態
矢崎こゆき,安藤友子,黒澤尋
第63回日本生物工学会大会講演要旨集 2011.9 日本生物工学会
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ハンギングドロップ法における培養条件がマウスES細胞胚様体の分化状態に及ぼす影響
大貫喜嗣,黒澤尋
第63回日本生物工学会大会講演要旨集 2011.9 日本生物工学会
Industrial Property Rights
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胚様体形成容器及び胚様体形成方法
黒澤 尋,榊 秀次郎
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胚性幹細胞（ES細胞）の胚様体（EB）形成のための培養容器及び培養方法
黒澤 尋,今村哲也,佐々木克典
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細胞の培養容器及び細胞の培養方法
黒澤 尋
Awards
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The 16th Award of Journal of Bioscience and Bioengineering
2008.8
Teaching Experience (On-campus)
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Advanced Cell Culture Engineering Major achievement
2005Year Type of subject：Dr. (Graduate School)
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生物反応工学
2005Year Type of subject：Professional education (undergraduate)
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微分方程式I
2005Year Type of subject：Professional education (undergraduate)
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バイオリアクター特論
2005Year Type of subject：Master's (Graduate School)
Other educational achievements
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発生工学技術開発・実践特別教育プログラム
2018
Social Activities
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教育セミナー「培養技術勉強会」
Role(s)： Lecturer
日本生物工学会 2022.3
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教育セミナー「培養技術勉強会」
Role(s)： Lecturer
日本生物工学会 2021.8
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The Society for Biotechnology, Japan Editor of Journal of Bioscience and Bioengineering
2009.6 - 2013.5
Professional Memberships
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日本組織培養学会
2018.4
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Japanese Society for Regenerative Medicine
2002.4
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Japanese Association for Animal Cell Technology
1996.9
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Society of Chemical Engineers, Japan
1988.9
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Society for Biotechnology, Japan
1985.9